Y. Kawarasaki

发表

Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.

Stephen J Benkovic, George Georgiou, Karl E Griswold, 2003, Nucleic acids research.

Evolution of highly active enzymes by homology-independent recombination.

Stephen J Benkovic, George Georgiou, Karl E Griswold, 2005, Proceedings of the National Academy of Sciences of the United States of America.

PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets

Takaaki Kojima, Hideo Nakano, Tsuneo Yamane, 2005, Nucleic acids research.

Identification of soybean peptide leginsulin variants in different cultivars and their insulin-like activities

Keisuke Ito, S. Ito, N. Miyoshi, 2018, Scientific Reports.

Identification and distinct regulation of three di/tripeptide transporters in Aspergillus oryzae.

Keisuke Ito, K. Gomi, Y. Kawarasaki, 2020, Bioscience, biotechnology, and biochemistry.

High cell-density expression system: a novel method for extracellular production of difficult-to-express proteins.

T. Yamane, Keisuke Ito, Y. Kawarasaki, 2012, Journal of bioscience and bioengineering.

Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.

S. Iwata, T. Misaka, K. Abe, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Purification and some properties of wheat germ acid phosphatases

T. Yamane, H. Nakano, Y. Kawarasaki, 1996 .

Stabilization of affinity-tagged recombinant protein during/after its production in a cell-free system using wheat-germ extract.

T. Yamane, H. Nakano, Yasuhiro Yamada, 2003, Journal of bioscience and bioengineering.

Highly productive cell-free protein synthesis system using condensed wheat-germ extract

T. Yamane, H. Nakano, Y. Kawarasaki, 1996 .

A long-lived batch reaction system of cell-free protein synthesis.

T. Yamane, H. Nakano, Y. Kawarasaki, 1995, Analytical biochemistry.

Prolonged cell-free protein synthesis in a batch system using wheat germ extract.

T. Yamane, H. Nakano, Y. Kawarasaki, 1994, Bioscience, biotechnology, and biochemistry.

An increased rate of cell-free protein synthesis by condensing wheat-germ extract with ultrafiltration membranes.

T. Yamane, H. Nakano, Y. Kawarasaki, 1994, Bioscience, biotechnology, and biochemistry.

Signal peptide optimization tool for the secretion of recombinant protein from Saccharomyces cerevisiae.

H. Nakano, T. Sahara, S. Ohgiya, 2015, Journal of bioscience and bioengineering.

Cloning and in vitro and in vivo expression of plant glutathione S-transferase zeta class genes.

T. Yamane, H. Nakano, Y. Kawarasaki, 2003, Journal of bioscience and bioengineering.

Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans.

S. Iwata, A. Cameron, T. Misaka, 2011, Journal of molecular biology.

Export Pathway Selectivity of Escherichia coli Twin Arginine Translocation Signal Peptides*

George Georgiou, Danielle Tullman-Ercek, Tracy Palmer, 2007, Journal of Biological Chemistry.

Soy Peptides Enhance Heterologous Membrane Protein Productivity during the Exponential Growth Phase of Saccharomyces cerevisiae

T. Misaka, K. Abe, Keisuke Ito, 2012, Bioscience, biotechnology, and biochemistry.

Analysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library

Ryuji Kato, Keisuke Ito, Y. Kawarasaki, 2013, Nature Communications.

Analyzing a dipeptide library to identify human dipeptidyl peptidase IV inhibitor.

Keisuke Ito, S. Ito, Y. Kawarasaki, 2015, Food chemistry.

Regulation of RraA, a Protein Inhibitor of RNase E-Mediated RNA Decay

G. Georgiou, Li Zhou, Y. Kawarasaki, 2006, Journal of bacteriology.

High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression.

Hideo Nakano, Tsuneo Yamane, Suang Rungpragayphan, 2002, Journal of molecular biology.

Emulsion culture: a miniaturized library screening system based on micro-droplets in an emulsified medium.

M. Nakajima, H. Nakano, Teruyo Ojima, 2011, Journal of bioscience and bioengineering.

A method for reverse interactome analysis: High‐resolution mapping of interdomain interaction network in Dam1 complex and its specific disorganization based on the interaction domain expression

T. Yamane, A. Ikeuchi, H. Nakano, 2010, Biotechnology progress.

High cell-density expression system: yeast cells in a phalanx efficiently produce a certain range of "difficult-to-express" secretory recombinant proteins.

Keisuke Ito, Y. Kawarasaki, Takeshi Kurose, 2015, Methods in molecular biology.

A periplasmic fluorescent reporter protein and its application in high-throughput membrane protein topology analysis.

George Georgiou, G. Georgiou, B. Iverson, 2004, Journal of molecular biology.

Identification of soybean peptide leginsulin variants in different cultivars and their insulin-like activities

Keisuke Ito, S. Ito, N. Miyoshi, 2018, Scientific Reports.

Chimeric Gene Library Construction by a Simple and Highly Versatile Method Using Recombination‐Dependent Exponential Amplification

Tsuneo Yamane, T. Yamane, A. Ikeuchi, 2003, Biotechnology progress.

Phosphatase-immunodepleted cell-free protein synthesis system.

T. Yamane, H. Nakano, Y. Kawarasaki, 1998, Journal of biotechnology.

Synergistic inhibition of human dipeptidyl peptidase IV by combinations of peptides

Keisuke Ito, M. Kusubata, S. Ito, 2015, Peptides.

Systematic analysis of a dipeptide library for inhibitor development using human dipeptidyl peptidase IV produced by a Saccharomyces cerevisiae expression system.

Ryuji Kato, Keisuke Ito, Y. Kawarasaki, 2013, Biochemical and biophysical research communications.

A Trimmed Viral Cap‐Independent Translation Enhancing Sequence for Rapid in Vitro Gene Expression

T. Yamane, H. Nakano, Y. Kawarasaki, 2000, Biotechnology progress.

Recent Advances in Cell‐free Protein Synthesis towards a Protein Biosynthesizer a

T. Yamane, H. Nakano, Y. Kawarasaki, 1996 .

Insight of diagnostic performance using B-cell epitope antigens derived from triple P44-related proteins of Anaplasma phagocytophilum.

Keisuke Ito, N. Ohashi, S. Ando, 2019, Diagnostic microbiology and infectious disease.

Secretory expression of Lentinula edodes intracellular laccase by yeast high-cell-density system: sub-milligram production of difficult-to-express secretory protein.

Keisuke Ito, Y. Kawarasaki, Y. Nakagawa, 2014, Journal of bioscience and bioengineering.

High cell-density expression system: a novel method for extracellular production of difficult-to-express proteins.

T. Yamane, Keisuke Ito, Y. Kawarasaki, 2012, Journal of bioscience and bioengineering.

Exhaustive identification of interaction domains using a high-throughput method based on two-hybrid screening and PCR-convergence: molecular dissection of a kinetochore subunit Spc34p.

Tsuneo Yamane, T. Yamane, A. Ikeuchi, 2003, Nucleic acids research.

Trp-Arg-Xaa tripeptides act as uncompetitive-type inhibitors of human dipeptidyl peptidase IV

Keisuke Ito, S. Ito, Y. Kawarasaki, 2014, Peptides.

Quantitative Y2H screening: cloning and signal peptide engineering of a fungal secretory LacA gene and its application to yeast two-hybrid system as a quantitative reporter.

H. Nakano, Teruyo Ojima, Y. Kawarasaki, 2010, Journal of biotechnology.