T. Hohsaka

发表

Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs

Hiroshi Murakami, Takahiro Hohsaka, Masahiko Sisido, 2002, Nature Biotechnology.

Synthesis of Novel BRET/FRET Protein Probes Containing Light-Emitting Proteins and Fluorescent Nonnatural Amino Acids

Takahiro Hohsaka, Atsushi Yamaguchi, T. Hohsaka, 2012 .

Different protein localizations on the inner and outer leaflet of cell-sized liposomes using cell-free protein synthesis

T. Matsuura, Takayoshi Watanabe, T. Hohsaka, 2018, Synthetic biology.

Liposome‐Based in Vitro Evolution of Aminoacyl‐tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation

T. Yomo, T. Matsuura, Yasuhiko Kato, 2015, Chembiochem : a European journal of chemical biology.

Antigen-responsive fluorescent antibody probes generated by selective N-terminal modification of IgGs.

Ryoji Abe, Takayoshi Watanabe, T. Hohsaka, 2018, Chemical communications.

Preparation of glycosylated amino acid derivatives for glycoprotein synthesis by in vitro translation system.

M. Sisido, Yukishige Ito, S. Manabe, 2002, Bioorganic & medicinal chemistry.

Freezing-Assisted Gene Delivery Combined with Polyampholyte Nanocarriers.

K. Matsumura, Takayoshi Watanabe, T. Hohsaka, 2017, ACS biomaterials science & engineering.

A Cell-Free Translocation System Using Extracts of Cultured Insect Cells to Yield Functional Membrane Proteins

Yoko Sato, T. Utsumi, N. Uozumi, 2014, PloS one.

Incorporation of Two Different Nonnatural Amino Acids Independently into a Single Protein through Extension of the Genetic Code

M. Sisido, T. Hohsaka, H. Sasaki, 1999 .

Facile aminoacylation of pdCpA dinucleotide with a nonnatural amino acid in cationic micelle.

M. Sisido, K. Ninomiya, T. Hohsaka, 2003, Chemical communications.

Cell-Free Synthesis of Human Endothelin Receptors and Its Application to Ribosome Display.

O. Nureki, Yoshihiro Shimizu, T. Yamaguchi, 2022, Analytical chemistry.

Novel biosensor system model based on fluorescence quenching by a fluorescent streptavidin and carbazole‐labeled biotin

R. Miyatake, H. Shinohara, T. Hohsaka, 2016, Journal of molecular recognition : JMR.

A novel molecular biosensor system model designed by using a couple composed of a fluorescent mutant binding protein and fluorophore-labeled ligand analogue based on the FRET mechanism

H. Shinohara, T. Hohsaka, Xianwei Zhu, 2015 .

Antibody-based fluorescent and fluorescent ratiometric indicators for detection of phosphotyrosine.

Takayoshi Watanabe, T. Hohsaka, K. P. Huynh Nhat, 2016, Journal of bioscience and bioengineering.

A non‐natural amino acid for efficient incorporation into proteins as a sensitive fluorescent probe

M. Sisido, H. Murakami, K. Taira, 2001, FEBS letters.

Adaptability of nonnatural aromatic amino acids to the active center of the E. coli ribosomal A site

S. Yokoyama, M. Sisido, T. Hohsaka, 1993, FEBS letters.

Molecular design of glycoprotein mimetics: glycoblotting by engineered proteins with an oxylamino-functionalized amino acid residue.

H. Kondo, K. Niikura, S. Nishimura, 2005, Chemistry.

Incorporation of Non-Natural Amino Acids with Two Labeling Groups into the N-Terminus of Proteins

T. Chiba, T. Hohsaka, I. Iijima, 2010 .

FRET analysis of protein conformational change through position-specific incorporation of fluorescent amino acids

M. Sisido, Ryoji Abe, T. Hohsaka, 2006, Nature Methods.

Efficient Incorporation of Nonnatural Amino Acids with Large Aromatic Groups into Streptavidin in In Vitro Protein Synthesizing Systems

M. Sisido, T. Hohsaka, Y. Ashizuka, 1999 .

Five-base codons for incorporation of nonnatural amino acids into proteins.

M. Sisido, H. Murakami, T. Hohsaka, 2001, Nucleic acids research.

Incorporation of nonnatural amino acids into proteins by using various four-base codons in an Escherichia coli in vitro translation system.

M. Sisido, H. Murakami, T. Hohsaka, 2001, Biochemistry.

Incorporation of Nonnatural Amino Acids into Streptavidin through In Vitro Frame-Shift Suppression

M. Sisido, H. Murakami, T. Hohsaka, 1996 .

Position-specific incorporation of biotinylated non-natural amino acids into a protein in a cell-free translation system.

Takayoshi Watanabe, T. Hohsaka, I. Iijima, 2007, Biochemical and biophysical research communications.

Four-base codon/anticodon strategy and non-enzymatic aminoacylation for protein engineering with non-natural amino acids.

T. Ohtsuki, M. Sisido, K. Ninomiya, 2005, Methods.

Photoswitching of NAD+-mediated enzyme reaction through photoreversible antigen-antibody reaction

M. Sisido, T. Hohsaka, Kazuo Kawashima, 1994 .

Sense codon-dependent introduction of unnatural amino acids into multiple sites of a protein.

M. Sisido, H. Takaku, T. Hohsaka, 2000, Biochemical and Biophysical Research Communications - BBRC.

Extension of Protein Functions by the Incorporation of Nonnatural Amino Acids

M. Sisido, T. Hohsaka, 1999 .

Site-Directed Incorporation of p-Nitrophenylalanine into Streptavidin and Site-to-Site Photoinduced Electron Transfer from a Pyrenyl Group to a Nitrophenyl Group on the Protein Framework

M. Sisido, H. Murakami, T. Hohsaka, 1998 .

Site-specific incorporation of PEGylated amino acids into proteins using nonnatural amino acid mutagenesis.

T. Hohsaka, I. Iijima, Naoki Shozen, 2009, Bioorganic & medicinal chemistry letters.

In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system

M. Sisido, T. Hohsaka, H. Taira, 2006, Nucleic acids research.

Farnesyl pyrophosphate regulates adipocyte functions as an endogenous PPARγ agonist

T. Kawada, Hiroyuki Nagai, S. Ebisu, 2011, The Biochemical journal.

Statistical description of the denatured structure of a single protein, staphylococcal nuclease, by FRET analysis

H. Kamikubo, M. Kataoka, Takayoshi Watanabe, 2017, Biophysical Reviews.

Four-base codon-mediated saturation mutagenesis in a cell-free translation system.

Takayoshi Watanabe, T. Hohsaka, Norihito Muranaka, 2008, Journal of bioscience and bioengineering.

Incorporation of non-natural amino acids into proteins.

Takahiro Hohsaka, Masahiko Sisido, M. Sisido, 2002, Current opinion in chemical biology.

pH-sensitive locomotion of cyclodextrins in a block-selective mobile polyrotaxane.

H. Choi, N. Yui, T. Ooya, 2006, Chemphyschem : a European journal of chemical physics and physical chemistry.

Random insertion and deletion mutagenesis.

Hiroshi Murakami, Takahiro Hohsaka, Masahiko Sisido, 2003, Methods in molecular biology.

Position‐specific incorporation of dansylated non‐natural amino acids into streptavidin by using a four‐base codon

M. Sisido, H. Murakami, Ryoji Abe, 2004, FEBS letters.

Site-directed incorporation of fluorescent nonnatural amino acids into streptavidin for highly sensitive detection of biotin.

M. Sisido, H. Murakami, T. Hohsaka, 2000, Biomacromolecules.

Chemical Synthesis and Cell-Free Expression of Thiazoline Ring-Bridged Cyclic Peptides and Their Properties on Biomembrane Permeability

T. Tamura, T. Hohsaka, Yuji Yoshimitsu, 2022, Bulletin of the Chemical Society of Japan.

"Quenchbodies": quench-based antibody probes that show antigen-dependent fluorescence.

Takahiro Hohsaka, Issei Iijima, Hiroshi Ueda, 2011, Journal of the American Chemical Society.

Site-Specific Incorporation of Fluorescent Nonnatural Amino Acids into Proteins and Its Application to Fluorescence Analysis of Proteins

T. Hohsaka, 2016 .

Position‐Specific Incorporation of Fluorescent Non‐natural Amino Acids into Maltose‐Binding Protein for Detection of Ligand Binding by FRET and Fluorescence Quenching

T. Hohsaka, I. Iijima, 2009, Chembiochem : a European journal of chemical biology.

Incorporation of fluorescent non-natural amino acids into N-terminal tag of proteins in cell-free translation and its dependence on position and neighboring codons.

S. Ebisu, Ryoji Abe, T. Hohsaka, 2010, Journal of bioscience and bioengineering.

Incorporation of glycosylated amino acid into protein by an in vitro translation system.

Toshinori Sato, T. Matsubara, Kazutoshi Iijima, 2013, Bioorganic & medicinal chemistry letters.

Photoswitching of peroxidase activity by position‐specific incorporation of a photoisomerizable non‐natural amino acid into horseradish peroxidase

M. Sisido, T. Hohsaka, Norihito Muranaka, 2002, FEBS letters.

Position-specific incorporation of a fluorophore-quencher pair into a single streptavidin through orthogonal four-base codon/anticodon pairs.

M. Sisido, H. Murakami, K. Taira, 2002, Journal of the American Chemical Society.

Introduction of specialty functions by the position-specific incorporation of nonnatural amino acids into proteins through four-base codon/anticodon pairs

M. Sisido, T. Hohsaka, 2001, Applied Microbiology and Biotechnology.

Site‐specific incorporation of photofunctional nonnatural amino acids into a polypeptide through in vitro protein biosynthesis

S. Yokoyama, M. Sisido, T. Hohsaka, 1994, FEBS letters.

Synthesis of Nonnatural mutants of λ-Cro repressor protein that contain an electron-accepting amino acid

M. Sisido, T. Hohsaka, S. Tokunaga, 2000 .

Bottom-up creation of an artificial cell covered with the adhesive bacterionanofiber protein AtaA.

T. Matsuura, Masahito Ishikawa, K. Hori, 2019, Journal of the American Chemical Society.

Incorporation of Fluorescent-Labeled Non-α-Amino Carboxylic Acids into the N-Terminus of Proteins in Response to Amber Initiation Codon

Ryoji Abe, T. Hohsaka, Norihito Muranaka, 2010 .

Double-Fluorescent-Labeled Single-Chain Antibodies Showing Antigen-Dependent Fluorescence Ratio Change

Takayoshi Watanabe, T. Hohsaka, Kensuke Yoshikoshi, 2016 .

Comprehensive screening of amber suppressor tRNAs suitable for incorporation of non-natural amino acids in a cell-free translation system.

K. Kojima, T. Hohsaka, Y. Matsushita, 2008, Biochemical and biophysical research communications.

Binding efficiency of elongation factor Tu to tRNAs charged with nonnatural fluorescent amino acids.

T. Ohtsuki, M. Sisido, Ryoji Abe, 2006, Analytical Biochemistry.

Incorporation of fluorescently labeled nonnatural amino acids into proteins in an E. coli in vitro translation system.

M. Sisido, Ryoji Abe, T. Hohsaka, 2003, Nucleic acids research. Supplement.

Incorporation of Fluorescent Nonnatural Amino Acid into Sialic Acid-Binding Lectin for Fluorescence Detection of Ligand-Binding

T. Hohsaka, Yasutaka Ito, 2013 .

Amber codon-mediated expanded saturation mutagenesis of proteins using a cell-free translation system.

Takayoshi Watanabe, T. Hohsaka, Naoki Shozen, 2012, Journal of bioscience and bioengineering.

Biosynthesis of proteins containing modified lysines and fluorescent labels using non-natural amino acid mutagenesis.

Ryoji Abe, Takayoshi Watanabe, T. Hohsaka, 2011, Journal of bioscience and bioengineering.

N‐Terminal Specific Fluorescence Labeling of Proteins through Incorporation of Fluorescent Hydroxy Acid and Subsequent Ester Cleavage

Ryoji Abe, Takayoshi Watanabe, T. Hohsaka, 2008, Chembiochem : a European journal of chemical biology.

Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates.

Takayoshi Watanabe, Shigeyuki Yokoyama, Yoshihisa Fujiwara, 2016, Bioconjugate chemistry.

Incorporation of glycosylated amino acid into protein by an in vitro translation system.

Toshinori Sato, T. Matsubara, Kazutoshi Iijima, 2013, Bioorganic & medicinal chemistry letters.

Four-base codon mediated mRNA display to construct peptide libraries that contain multiple nonnatural amino acids

M. Sisido, T. Hohsaka, Norihito Muranaka, 2006, Nucleic acids research.

Four-base codon-mediated incorporation of non-natural amino acids into proteins in a eukaryotic cell-free translation system.

M. Sisido, T. Hohsaka, M. Fukushima, 2005, Journal of bioscience and bioengineering.

Nonnatural mutagenesis in E. coli and rabbit reticulocyte lysates by using four-base codons.

M. Sisido, T. Hohsaka, M. Fukushima, 2002, Nucleic acids research. Supplement.

Synthesis and sequence optimization of GFP mutants containing aromatic non-natural amino acids at the Tyr66 position.

Takahiro Hohsaka, Masahiko Sisido, M. Sisido, 2005, Protein engineering, design & selection : PEDS.

Efficient synthesis of nonnatural mutants in Escherichia coli S30 in vitro protein synthesizing system.

M. Sisido, T. Hohsaka, Hidetaka Nakata, 2004, Journal of bioscience and bioengineering.

Incorporation of Unnatural Non‐α‐Amino Acids into the N Terminus of Proteins in a Cell‐Free Translation System

T. Hohsaka, Norihito Muranaka, M. Miura, 2007, Chembiochem : a European journal of chemical biology.

Incorporation of unnatural non-α-amino acids into the N-terminus of proteins in a cell-free translation system.

T. Hohsaka, 2012, Methods in molecular biology.