A. Gilep

发表

Direct Molecular Fishing of Protein Partners for Proteins Encoded by Genes of Human Chromosome 18 in HepG2 Cell Lysate

A. Ivanov, V. Zgoda, O. Gnedenko, 2018, Russian journal of bioorganic chemistry.

Development of CYB5-fusion monitoring system for efficient periplasmic expression of multimeric proteins in Escherichia coli.

A. Gilep, S. Usanov, D. Dormeshkin, 2016, Protein expression and purification.

Human Lanosterol 14-Alpha Demethylase (CYP51A1) Is a Putative Target for Natural Flavonoid Luteolin 7,3′-Disulfate

A. Ivanov, A. Popov, O. Gnedenko, 2021, Molecules.

Assessment of electrocatalytic hydroxylase activity of cytochrome P450 3A4 (CYP3A4) by means of derivatization of 6β-hydroxycortisol by sulfuric acid for fluorimetric assay.

A. Archakov, A. Kuzikov, V. Shumyantseva, 2019, Talanta.

Interaction of Isatin with Cytochrome P450 Isoenzymes: Investigation by Means of Spectral and Electrochemical Methods The role of Isatin in Cytochromes P450 Ligand-Protein Binding Events

A. Ivanov, A. Kuzikov, Y. Mezentsev, 2020, BioNanoScience.

Hydroxylation of Antitubercular Drug Candidate, SQ109, by Mycobacterial Cytochrome P450

Sergei Grudinin, A. Gilep, N. Strushkevich, 2020, International journal of molecular sciences.

Application of the SPR Biosensor for the Analysis of Protein–Protein Interactions in Aqueous Environment and Bilayer Lipid Membrane As Exemplified by P450scc (CYP11A1)

A. Ivanov, O. Gnedenko, P. Ershov, 2021 .

Computer-aided design of aptamers for cytochrome p450.

A. Archakov, A. Veselovsky, A. Ivanov, 2015, Journal of structural biology.

Electrochemical oxidation of estrogens as a method for CYP19A1 (aromatase) electrocatalytic activity determination

A. Kuzikov, V. Shumyantseva, A. Gilep, 2020 .

Electrochemical characterization of mutant forms of rubredoxin B from Mycobacterium tuberculosis.

F. Schacher, D. Pergushov, A. Kuzikov, 2021, Biochimica et biophysica acta. Proteins and proteomics.

Interaction of 17α-hydroxylase, 17(20)-lyase (CYP17A1) inhibitors - abiraterone and galeterone - with human sterol 14α-demethylase (CYP51A1).

A. Archakov, A. Veselovsky, A. Kuzikov, 2018, Journal of inorganic biochemistry.

Substrate-induced modulation of protein-protein interactions within human mitochondrial cytochrome P450-dependent system

A. Ivanov, Y. Mezentsev, P. Ershov, 2020, The Journal of Steroid Biochemistry and Molecular Biology.

Proteomic analysis of contaminants in recombinant membrane hemeproteins expressed in E. coli and isolated by metal affinity chromatography

A. Gilep, S. Usanov, M. Shapiro, 2017, Applied Biochemistry and Microbiology.

A new twist of rubredoxin function in M. tuberculosis.

K. Tsumoto, A. Kuzikov, V. Shumyantseva, 2021, Bioorganic chemistry.

A new twist of rubredoxin function in M. tuberculosis

K. Tsumoto, A. Kuzikov, V. Shumyantseva, 2020 .

Bioelectrochemical Systems as Technologies for Studying Drug Interactions Related to Cytochrome P450

A. Archakov, A. Kuzikov, T. Bulko, 2018, BioNanoScience.

Identification of Mycobacterium tuberculosis enzyme involved in vitamin D and 7-dehydrocholesterol metabolism

A. Vasilevskaya, A. Gilep, S. Usanov, 2017, The Journal of Steroid Biochemistry and Molecular Biology.

Adrenodoxin supports reactions catalyzed by microsomal steroidogenic cytochrome P450s.

A. Gilep, S. Usanov, I. Grabovec, 2007, Biochemical and biophysical research communications.

Engineering of proteolytically stable NADPH-cytochrome P450 reductase

R. Estabrook, A. Gilep, S. Usanov, 2005, Biochemistry (Moscow).

Site-Directed Mutagenesis of Cytochrome P450scc. II. Effect of Replacement of the Arg425 and Arg426 Residues on the Structural and Functional Properties of the Cytochrome P450scc

G. Lepesheva, A. Gilep, S. Usanov, 2001, Biochemistry (Moscow).

Chimerogenesis in Estimation of Specificity and Pathway Directions for Cytochrome P45017α Catalyzed Reactions

R. Estabrook, A. Gilep, S. Usanov, 2004, Biochemistry (Moscow).

Human Cytochrome P450 2C9 and Its Polymorphic Modifications: Electroanalysis, Catalytic Properties, and Approaches to the Regulation of Enzymatic Activity

T. Bulko, V. Shumyantseva, A. Gilep, 2022, Processes.

Specificity of Isatin Interaction with Cytochromes P450

A. Ivanov, Y. Mezentsev, P. Ershov, 2018, Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry.

[The effect of isatin on protein-protein interactions between cytochrome b5 and cytochromes P450].

A. Veselovsky, A. Ivanov, O. Gnedenko, 2017, Biomeditsinskaia khimiia.

THE COMPARATIVE EFFICACY OF PERSONALIZING THERAPY WITH ANTICONVULSANTS AND ANTIPSYCHOTICS ON THE BASIS OF PHARMACOGENETIC TESTING

A. I. Startsev, T. Golubeva, A. Gilep, 2016 .

Molecular modeling of human lanosterol 14α-demethylase complexes with substrates and their derivatives

S. Feranchuk, A. Gilep, S. Usanov, 2011, Biochemistry (Moscow).

Screening of Potential Non-Azole Inhibitors of Lanosterol 14-Alpha Demethylase (CYP51) of the Сandida Fungi

A. Ivanov, O. Gnedenko, Y. Mezentsev, 2021, Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry.

Reconstitution of the enzymatic activities of cytochrome P450s using recombinant flavocytochromes containing rat cytochrome b(5) fused to NADPH--cytochrome P450 reductase with various membrane-binding segments.

R. Estabrook, A. Gilep, S. Usanov, 2001, Archives of biochemistry and biophysics.

In vitro interactions of abiraterone, erythromycin, and CYP3A4: implications for drug–drug interactions

D. Filimonov, A. Archakov, A. Veselovsky, 2020, Fundamental & clinical pharmacology.

Human Cytochrome P450 2C9 and its Polymorphic Modifications: Electroanalysis, Catalytic Properties and Approaches to the Regulation of Enzymatic Activity

T. Bulko, V. Shumyantseva, A. Gilep, 2022, SSRN Electronic Journal.

Facile bulk preparation and structural characterization of agglomerated γ-Fe2O3/SiO2 nanocomposite particles for nucleic acids isolation and analysis

D. Kotsikau, A. Gilep, V. Pankov, 2018, Materials Chemistry and Physics.

Structural insights into 3Fe–4S ferredoxins diversity in M. tuberculosis highlighted by a first redox complex with P450

K. Tsumoto, V. Gordeliy, A. Ivanov, 2022, bioRxiv.

Probing the interaction of bovine cytochrome P450scc (CYP11A1) with adrenodoxin: evaluating site-directed mutations by molecular modeling.

R. Estabrook, J. Peterson, G. Lepesheva, 2002, Biochemistry.

Interaction of apo-cytochrome b5 with cytochromes P4503A4 and P45017A: relevance of heme transfer reactions.

R. Estabrook, A. Gilep, S. Usanov, 2001, Biochemistry.

All-electrochemical nanocomposite two-electrode setup for quantification of drugs and study of their electrocatalytical conversion by cytochromes P450

I. Romanenko, F. Schacher, D. Pergushov, 2020 .

A new insight into subinteractomes of functional antagonists: Thromboxane (CYP5A1) and prostacyclin (CYP8A1) synthases

V. Zgoda, O. Gnedenko, P. Ershov, 2021, Cell biology international.

An effective scheme to produce recombinant uracil-DNA glycosylase of Escherichia coli for PCR diagnostics

A. Gilep, S. Usanov, A. Yantsevich, 2014, Applied Biochemistry and Microbiology.

PharmVar GeneFocus: CYP3A5

M. Whirl‐Carrillo, K. Sangkuhl, T. Klein, 2022, Clinical pharmacology and therapeutics.

Expression of outer mitochondrial membrane cytochrome b5 in Escherichia coli. Purification of the recombinant protein and studies of its interaction with electron-transfer partners

R. Estabrook, A. Gilep, S. Usanov, 2006, Biochemistry (Moscow).

Different inhibitory effects of azole-containing drugs and pesticides on CYP2C9 polymorphic forms: An in vitro study.

A. Gilep, S. Usanov, I. Grabovec, 2018, Toxicology in vitro : an international journal published in association with BIBRA.

Screening of human cytochrome P450(51) (CYP51A1) inhibitors: Structural lanosterol analogues of plant and animal origin

A. Lisitsa, A. Ivanov, V. Stonik, 2014, Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry.

Structural Analogues of Lanosterol from Marine Organisms of the Class Asteroidea as Potential Inhibitors of Human and Candida albicans Lanosterol 14α-demethylases

A. Ivanov, L. Kaluzhskiy, A. Gilep, 2017 .

The SPR-based biosensor test system for analysis of small compounds interaction with human cytochrome P450 51A1 (CYP51A1)

A. Lisitsa, A. Archakov, A. Ivanov, 2013, Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry.

Bioelectrochemical Systems as Technologies for Studying Drug Interactions Related to Cytochrome P450

A. Archakov, A. Kuzikov, T. Bulko, 2018, BioNanoScience.

Molecular cloning, heterological expression and production of the human growth hormone

A. Gilep, G. Sergeev, A. Gorkavaya, 2019, Proceedings of the National Academy of Sciences of Belarus, Chemical Series.

Direct molecular fishing in molecular partners investigation in protein–protein and protein–peptide interactions

A. Makarov, V. Mitkevich, A. Ivanov, 2016, Russian Journal of Bioorganic Chemistry.

The development of an efficient system for heterologous expression of cytochrome P450s in Escherichia coli using hemA gene co-expression.

A. Gilep, S. Usanov, Andrei A Gilep, 2006, Protein expression and purification.

A large-scale comparative analysis of affinity, thermodynamics and functional characteristics of interactions of twelve cytochrome P450 isoforms and their redox partners.

A. Ivanov, O. Gnedenko, P. Ershov, 2019, Biochimie.

Thermodynamics of interactions between mammalian cytochromes P450 and b5.

A. Ivanov, A. Florinskaya, A. Gilep, 2017, Archives of biochemistry and biophysics.

The role of cytochrome b5 structural domains in interaction with cytochromes P450

A. Gilep, S. Usanov, G. Sergeev, 2014, Biochemistry (Moscow).

At the crossroads of steroid hormone biosynthesis: the role, substrate specificity and evolutionary development of CYP17.

A. Gilep, S. Usanov, T. Sushko, 2011, Biochimica et biophysica acta.

Structural insights into aldosterone synthase substrate specificity and targeted inhibition.

C. Arrowsmith, Hee-won Park, A. Gilep, 2013, Molecular endocrinology.

Molecular Cloning and Heterologous Expression in E. coli of Cytochrome P45017α. Comparison of Structural and Functional Properties of Substrate-Specific Cytochromes P450 from Different Species

R. Estabrook, A. Gilep, S. Usanov, 2004, Biochemistry (Moscow).

The stimulatory role of human cytochrome b5 in the bioactivation activities of human CYP1A2, 2A6 and 2E1: a new cell expression system to study cytochrome P450-mediated biotransformation (a corrigendum report on Duarte et al. (2005) Mutagenesis 20, 93-100).

José Rueff, M. P. Duarte, A. Gilep, 2006, Mutagenesis.

Engineering, expression, purification, and physicochemical characterization of a chimeric protein, full-length cytochrome b5-green fluorescence protein (HMWb5-EGFP)

A. Gilep, S. Usanov, A. Yantsevich, 2007, Biochemistry (Moscow).

Site-Directed Mutagenesis of Cytochrome P450scc (CYP11A1). Effect of Lysine Residue Substitution on Its Structural and Functional Properties

G. Lepesheva, A. Gilep, S. Usanov, 2000, Biochemistry (Moscow).

Molecular identification of adrenal inner zone antigen as a heme‐binding protein

G. Vinson, H. Hori, M. Okamoto, 2005, The FEBS journal.

Mechanism of intermolecular interactions of microsomal cytochrome P450s CYP17 and CYP21 involved in steroid hormone biosynthesis

A. Gilep, S. Usanov, T. Sushko, 2012, Biochemistry (Moscow).

Mechanism of the Affinity-Enhancing Effect of Isatin on Human Ferrochelatase and Adrenodoxin Reductase Complex Formation: Implication for Protein Interactome Regulation

A. Veselovsky, A. Ivanov, Y. Mezentsev, 2020, International journal of molecular sciences.

Electron transfer properties and catalytic competence of cytochrome b5 in the fusion protein Hmwb5-EGFP in reactions catalyzed by cytochrome P450 3A4

A. Gilep, S. Usanov, A. Yantsevich, 2009, Biochemistry (Moscow).

Role of microsomal steroid hydroxylases in Δ7-steroid biosynthesis

A. Gilep, S. Usanov, T. Sushko, 2013, Biochemistry (Moscow).

Hydroxylation of Antitubercular Drug Candidate, SQ109, by Mycobacterial Cytochrome P450

Sergei Grudinin, A. Gilep, N. Strushkevich, 2020, bioRxiv.

Affinity Isolation and Mass Spectrometry Identification of Prostacyclin Synthase (PTGIS) Subinteractome

A. Ivanov, A. Kopylov, Y. Mezentsev, 2019, Biology.

Human steroid and oxysterol 7α‐hydroxylase CYP7B1: substrate specificity, azole binding and misfolding of clinically relevant mutants

F. Mackenzie, A. Gilep, S. Usanov, 2014, The FEBS journal.

Involvement of HxuC Outer Membrane Protein in Utilization of Hemoglobin by Haemophilus influenzae

R. Estabrook, E. Hansen, L. Cope, 2001, Infection and Immunity.

Metabolic fate of human immunoactive sterols in Mycobacterium tuberculosis.

A. Gabrielian, A. Rosenthal, V. Gordeliy, 2020, Journal of molecular biology.

Metabolic fate of human immunoactive sterols in Mycobacterium tuberculosis

A. Gabrielian, A. Rosenthal, V. Gordeliy, 2020, bioRxiv.

Mechanism of electron transfer in fusion protein cytochrome b5—NADH-cytochrome b5 reductase

A. Gilep, S. Usanov, A. Yantsevich, 2008, Biochemistry (Moscow).

Increasing the Efficiency of Cytochrome P450 3A4 Electrocatalysis Using Electrode Modification with Spatially Ordered Anodic Aluminum Oxide-Based Nanostructures for Investigation of Metabolic Transformations of Drugs

Y. Ivanov, K. Napolskii, V. Shumyantseva, 2022, Doklady Biochemistry and Biophysics.

No effect of lipoic acid on catalytic activity of cytochrome P450 3A4

T. Bulko, V. Shumyantseva, A. Gilep, 2020, Drug metabolism and personalized therapy.

Apo-cytochrome b5 as an indicator of changes in heme accessability: preliminary studies with cytochrome P450 3A4.

R. Estabrook, A. Gilep, S. Usanov, 2001, Journal of inorganic biochemistry.

Structural characterization of human cholesterol 7α-hydroxylase

F. Mackenzie, Hee-won Park, A. Gilep, 2014, Journal of Lipid Research.

Improving the Efficiency of Electrocatalysis of Cytochrome P450 3A4 by Modifying the Electrode with Membrane Protein Streptolysin O for Studying the Metabolic Transformations of Drugs

V. Shumyantseva, A. Gilep, S. V. Kraevsky, 2023, Biosensors.

Approaches for increasing the electrocatalitic efficiency of cytochrome P450 3A4.

A. Veselovsky, T. Bulko, V. Shumyantseva, 2022, Bioelectrochemistry.

Cytochrome P450 3A4 as a Drug Metabolizing Enzyme: the Role of Sensor System Modifications in Electocatalysis and Electroanalysis

S. Babkina, A. Kuzikov, T. Bulko, 2020, Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry.

Modeling of Drug-Drug Interactions between Omeprazole and Erythromycin in the Cytochrome P450-Dependent System In vitro

D. Filimonov, A. Kuzikov, A. Dmitriev, 2021, Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry.

[Electroanalytical and electrocatalytical characteristics of cytochrome P450 3A4 using electrodes modified with nanocomposite carbon nanomaterials].

S. Babkina, A. Kuzikov, T. Bulko, 2020, Biomeditsinskaia khimiia.

Engineering, expression, and purification of “soluble” human cytochrome P45017α and its functional characterization

A. Gilep, S. Usanov, T. A. Pechurskaya, 2008, Biochemistry (Moscow).

Site-Directed Mutagenesis of Cytochrome b5 for Studies of Its Interaction with Cytochrome P450

A. Gilep, S. Usanov, M. Chudaev, 2001, Biochemistry (Moscow).

[Study specificity of isatin interactions with P450 cytochromes].

A. Ivanov, Y. Mezentsev, P. Ershov, 2018, Biomeditsinskaia khimiia.

The stimulatory role of human cytochrome b5 in the bioactivation activities of human CYP1A2, 2A6 and 2E1: a new cell expression system to study cytochrome P450 mediated biotransformation.

José Rueff, M. P. Duarte, A. Gilep, 2005, Mutagenesis.

Combining of synthetic VHH and immune scFv libraries for pregnancy-associated glycoproteins ELISA development

A. Gilep, D. Dormeshkin, A. Kavaleuski, 2022, Applied Microbiology and Biotechnology.

Conformational stability of cytochrome b5, enhanced green fluorescent protein, and their fusion protein Hmwb5-EGFP

A. Gilep, S. Usanov, A. Yantsevich, 2009, Biochemistry (Moscow).

Direct Molecular Fishing of Protein Partners for Proteins Encoded by Genes of Human Chromosome 18 in HepG2 Cell Lysate

A. Ivanov, V. Zgoda, O. Gnedenko, 2018, Russian Journal of Bioorganic Chemistry.

New azole derivatives of [17(20)E]-21-norpregnene: Synthesis and inhibition of prostate carcinoma cell growth

A. Gilep, G. Morozevich, A. Misharin, 2019, Steroids.

A pathway for the metabolism of vitamin D3: Unique hydroxylated metabolites formed during catalysis with cytochrome P450scc (CYP11A1)

R. Estabrook, A. Gilep, S. Usanov, 2003, Proceedings of the National Academy of Sciences of the United States of America.

Biosensor selection of small compounds, modulating a complex formation between cytochrome P450s and NADPH-dependent P450 oxidoreductase

Y. Mezentsev, P. Ershov, E. Yablokov, 2020 .

At the crossroads of steroid hormone biosynthesis: the role, substrate specificity and evolutionary development of CYP17.

A. Gilep, S. Usanov, T. Sushko, 2011, Biochimica et biophysica acta.

Engineering, expression, and purification of “soluble” human cytochrome P45017α and its functional characterization

A. Gilep, S. Usanov, T. A. Pechurskaya, 2008, Biochemistry (Moscow).

An enzymatically active chimeric protein containing the hydrophilic form of NADPH-cytochrome P450 reductase fused to the membrane-binding domain of cytochrome b5.

R. Estabrook, A. Gilep, S. Usanov, 2001, Biochemical and biophysical research communications.

Expression, purification, and physical properties of recombinant flavocytochrome fusion proteins containing rat cytochrome b(5) linked to NADPH-cytochrome P450 reductase by different membrane-binding segments.

R. Estabrook, A. Gilep, S. Usanov, 2001, Archives of biochemistry and biophysics.