B. Seelig

发表

Selection and evolution of enzymes from a partially randomized non-catalytic scaffold

Burckhard Seelig, Jack W. Szostak, J. Szostak, 2007, Nature.

Genetic Code Evolution Investigated through the Synthesis and Characterisation of Proteins from Reduced‐Alphabet Libraries

Dana J Morrone, M. Newton, B. Seelig, 2019, Chembiochem : a European journal of chemical biology.

ZFN, TALEN and CRISPR-Cas9 mediated homology directed gene insertion in Arabidopsis: A disconnect between somatic and germinal cells.

D. Voytas, J. Baller, Qi-wei Shan, 2018, Journal of genetics and genomics = Yi chuan xue bao.

Extensive libraries of gene truncation variants generated by in vitro transposition

B. Seelig, L. Mills, Aleardo Morelli, 2017, Nucleic acids research.

Structure and dynamics of a primordial catalytic fold generated by in vitro evolution

Fa-An Chao, Gianluigi Veglia, Burckhard Seelig, 2012, Nature chemical biology.

In vitro selection of peptides and proteins - advantages of mRNA display.

M. Newton, B. Seelig, Yari Cabezas-Perusse, 2019, ACS synthetic biology.

Highly Diverse Protein Library Based on the Ubiquitous (β/α)8 Enzyme Fold Yields Well‐Structured Proteins through in Vitro Folding Selection

B. Seelig, John C. Haugner, Misha V. Golynskiy, 2013, Chembiochem : a European journal of chemical biology.

Heme-binding enables allosteric modulation in an ancient TIM-barrel glycosidase

J. A. Gavira, A. Romero‐Rivera, J. M. Sanchez-Ruiz, 2021, Nature communications.

Heme-binding enables allosteric modulation in an ancient TIM-barrel glycosidase

J. A. Gavira, A. Romero‐Rivera, J. M. Sanchez-Ruiz, 2020, Nature Communications.

High throughput sequencing of in vitro selections of mRNA-displayed peptides: data analysis and applications

I. Chen, C. Blanco, B. Seelig, 2020, Physical chemistry chemical physics : PCCP.

Cell Survival Enabled by Leakage of a Labile Metabolic Intermediate

J. M. Sanchez-Ruiz, B. Ibarra-Molero, L. Gutierrez-Rus, 2022, bioRxiv.

Nature-inspired engineering of an artificial ligase enzyme by domain fusion

Dana J Morrone, B. Seelig, Nisha Kanwar, 2022, Nucleic acids research.

Synthesis, incorporation efficiency, and stability of disulfide bridged functional groups at RNA 5'-ends.

M. Famulok, J. Nowick, P. Arora, 2000, Bioorganic & medicinal chemistry.

PacBio sequencing output increased through uniform and directional fivefold concatenation

I. Chen, C. Blanco, B. Seelig, 2021, Scientific Reports.

One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag.

Anthony D. Keefe, J. Szostak, A. Keefe, 2001, Protein expression and purification.

Site-specific modification of enzymatically synthesized RNA: Transcription initiation and Diels-Alder reaction

A. Jäschke, B. Seelig, 1997 .

mRNA display for the selection and evolution of enzymes from in vitro-translated protein libraries

Burckhard Seelig, B. Seelig, 2011, Nature Protocols.

In vitro evolution of enzymes.

Dana J Morrone, B. Seelig, John C. Haugner, 2013, Methods in molecular biology.

Multifunctional enzymes from reduced genomes – model proteins for simple primordial metabolism?

B. Seelig, 2017, Molecular microbiology.

Heteronuclear Adiabatic Relaxation Dispersion (HARD) for quantitative analysis of conformational dynamics in proteins.

S. Mangia, S. Michaeli, M. Garwood, 2012, Journal of magnetic resonance.

Thermostable Artificial Enzyme Isolated by In Vitro Selection

B. Seelig, John C. Haugner, Aleardo Morelli, 2014, PloS one.

Universal labeling of 5'-triphosphate RNAs by artificial RNA ligase enzyme with broad substrate specificity.

B. Seelig, John C. Haugner, 2013, Chemical communications.

Advances in the directed evolution of proteins.

Burckhard Seelig, B. Seelig, Michael D Lane, 2014, Current opinion in chemical biology.

Highly efficient recombinant production and purification of streptococcal cysteine protease streptopain with increased enzymatic activity.

B. Seelig, M. D. Lane, 2016, Protein expression and purification.

Enantioselective Ribozyme Catalysis of a Bimolecular Cycloaddition Reaction

Andres Jäschke, Burckhard Seelig, A. Jäschke, 2000 .

Architecture of a Diels-Alderase ribozyme with a preformed catalytic pocket.

E. Westhof, A. Jäschke, B. Seelig, 2004, Chemistry & biology.

Enantioselektive Ribozymkatalyse einer bimolekularen Cycloadditionsreaktion

A. Jäschke, B. Seelig, F. Stuhlmann, 2000 .

EasyDIVER: A Pipeline for Assembling and Counting High-Throughput Sequencing Data from In Vitro Evolution of Nucleic Acids or Peptides

I. Chen, C. Blanco, B. Seelig, 2020, Journal of Molecular Evolution.

High throughput sequencing of in vitro selections of mRNA-displayed peptides: data analysis and applications

I. Chen, C. Blanco, B. Seelig, 2020, Physical chemistry chemical physics : PCCP.

De novo enzymes: from computational design to mRNA display.

Burckhard Seelig, Misha V Golynskiy, B. Seelig, 2010, Trends in biotechnology.

In vitro evolution of artificial enzymes: method development and applications

B. Seelig, Misha V. Golynskiy, Haugner, 2014 .

An autocatalytic network for ribozyme self-construction.

B. Seelig, 2008, Nature chemical biology.

Nanodroplet-Based Reagent Delivery into Water-in-Fluorinated-Oil Droplets

S. Behrens, B. Seelig, T. Kitaguchi, 2023, Biosensors.

De novo proteins from random sequences through in vitro evolution.

B. Seelig, Kun-Hwa Lee, C. Tong, 2021, Current opinion in structural biology.