J. Mancheño

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Production and physicochemical properties of recombinant Lactobacillus plantarum tannase.

J. Curiel, B. de Las Rivas, R. Muñoz, 2009, Journal of agricultural and food chemistry.

Two-dimensional crystallization on lipid monolayers and three-dimensional structure of sticholysin II, a cytolysin from the sea anemone Stichodactyla helianthus.

J. J. Fernández, J. Gavilanes, J. Martín-Benito, 2000, Biophysical journal.

RNase U2 and alpha-sarcin: a study of relationships.

J. Gavilanes, J. Davies, J. Mancheño, 2001, Methods in enzymology.

Ribonuclease U2: cloning, production in Pichia pastoris and affinity chromatography purification of the active recombinant protein.

J. Gavilanes, J. Davies, J. Mancheño, 2000, FEMS microbiology letters.

Oligomerization of the cytotoxin alpha-sarcin associated with phospholipid membranes.

J. Gavilanes, J. Mancheño, V. de los Ríos, 1998, Molecular membrane biology.

Overproduction and purification of biologically active native fungal alpha-sarcin in Escherichia coli.

C. López-Otín, J. Gavilanes, J. Barbero, 1994, Gene.

Biochemical and structural studies of two tetrameric nucleoside 2'-deoxyribosyltransferases from psychrophilic and mesophilic bacteria: Insights into cold-adaptation.

A. Joachimiak, B. Nocek, J. Fernández‐Lucas, 2021, International journal of biological macromolecules.

2′-deoxyribosyltransferase from bacillus psychrosaccharolyticus: A mesophilic-like biocatalyst for the synthesis of modified nucleosides from a psychrotolerant bacterium

A. Fresco-Taboada, J. Fernández‐Lucas, M. Arroyo, 2018 .

2′-Deoxyribosyltransferase from Leishmania mexicana, an efficient biocatalyst for one-pot, one-step synthesis of nucleosides from poorly soluble purine bases

F. Gago, J. Fernández‐Lucas, P. A. Sánchez‐Murcia, 2017, Applied Microbiology and Biotechnology.

Bovine seminal ribonuclease destabilizes negatively charged membranes.

J. Gavilanes, G. D'alessio, J. Mancheño, 1994, Biochemical and biophysical research communications.

Food mustard allergen interaction with phospholipid vesicles.

J. Gavilanes, J. Mancheño, M. Villalba, 1994, European journal of biochemistry.

Partially folded states of the cytolytic protein sticholysin II.

J. Gavilanes, J. Mancheño, M. E. Lanio, 2001, Biochimica et biophysica acta.

Thermodynamic profiles of penicillin G hydrolysis catalyzed by wild-type and Met----Ala168 mutant penicillin acylases from Kluyvera citrophila.

J. Barbero, J. Pérez-Gil, J. Martín, 1990, Biochimica et biophysica acta.

Sticholysins, two pore-forming toxins produced by the Caribbean Sea anemone Stichodactyla helianthus: their interaction with membranes.

J. Mancheño, M. E. Lanio, C. Álvarez, 2009, Toxicon : official journal of the International Society on Toxinology.

A preliminary crystallographic study of recombinant NicX, an Fe(2+)-dependent 2,5-dihydroxypyridine dioxygenase from Pseudomonas putida KT2440.

J. García, E. Díaz, José I. Jiménez, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

1 1 2 3 Gene cloning , expression , and characterization of phenolic acid 4 decarboxylase from Lactobacillus brevis RM 84 5 6 7

J. Curiel, R. Muñoz, J. M. Landete, 2016 .

Characterization of the first feruloyl esterase from 3 Lactobacillus plantarum 4 5 6 7

R. Muñoz, J. Mancheño, M. Esteban-Torres, 2013 .

Characterization of a Feruloyl Esterase from Lactobacillus plantarum

B. de Las Rivas, R. Muñoz, J. Mancheño, 2013, Applied and Environmental Microbiology.

Gene cloning, expression, and characterization of phenolic acid decarboxylase from Lactobacillus brevis RM84

J. Curiel, R. Muñoz, J. M. Landete, 2010, Journal of Industrial Microbiology & Biotechnology.

The pURI family of expression vectors: a versatile set of ligation independent cloning plasmids for producing recombinant His-fusion proteins.

J. Curiel, B. de Las Rivas, R. Muñoz, 2011, Protein expression and purification.

A complementary microscopy analysis of Sticholysin II crystals on lipid films: Atomic force and transmission electron characterizations.

J. Gavilanes, J. Martín-Benito, L. Vázquez, 2006, Biophysical chemistry.

Mechanism of the leakage induced on lipid model membranes by the hemolytic protein sticholysin II from the sea anemone Stichodactyla helianthus.

J. Gavilanes, J. Mancheño, M. E. Lanio, 1998, European journal of biochemistry.

Crystallization and preliminary crystallographic analysis of the catalytic module of endolysin from Cp-7, a phage infecting Streptococcus pneumoniae.

R. Molina, J. Hermoso, I. Angulo, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

BzdR, a Repressor That Controls the Anaerobic Catabolism of Benzoate in Azoarcus sp. CIB, Is the First Member of a New Subfamily of Transcriptional Regulators*

J. García, E. Díaz, M. Carmona, 2005, Journal of Biological Chemistry.

Rational Design of a Thermostable 2′-Deoxyribosyltransferase for Nelarabine Production by Prediction of Disulfide Bond Engineering Sites

J. Fernández‐Lucas, J. Mancheño, J. Acosta, 2022, International journal of molecular sciences.

Characterization of an atypical, thermostable, organic solvent- and acid-tolerant 2′-deoxyribosyltransferase from Chroococcidiopsis thermalis

F. Gago, J. Fernández‐Lucas, P. A. Sánchez‐Murcia, 2018, Applied Microbiology and Biotechnology.

Kinetic study of the aggregation and lipid mixing produced by alpha-sarcin on phosphatidylglycerol and phosphatidylserine vesicles: stopped-flow light scattering and fluorescence energy transfer measurements.

J. Gavilanes, J. Mancheño, A. Martı́nez del Pozo, 1994, Biophysical journal.

Crystallization and preliminary crystallographic analysis of a novel haemolytic lectin from the mushroom Laetiporus sulphureus.

I. Goldstein, J. Hermoso, J. Mancheño, 2004, Acta crystallographica. Section D, Biological crystallography.

Two-Photon Fluorescence Anisotropy Imaging to Elucidate the Dynamics and the Stability of Immobilized Proteins.

M. Lillo, F. López‐Gallego, J. Mancheño, 2016, The journal of physical chemistry. B.

Characterization of a halotolerant lipase from the lactic acid bacteria Lactobacillus plantarum useful in food fermentations

R. Muñoz, B. Rivas, J. Mancheño, 2015 .

Production and characterization of a tributyrin esterase from Lactobacillus plantarum suitable for cheese lipolysis.

B. de Las Rivas, R. Muñoz, J. Mancheño, 2014, Journal of dairy science.

Characterization of a cold-active esterase from Lactobacillus plantarum suitable for food fermentations.

B. de Las Rivas, R. Muñoz, J. Mancheño, 2014, Journal of agricultural and food chemistry.

Food phenolics and lactic acid bacteria.

J. Curiel, B. de Las Rivas, R. Muñoz, 2009, International journal of food microbiology.

Cloning, production, purification and preliminary crystallographic analysis of a glycosidase from the food lactic acid bacterium Lactobacillus plantarum CECT 748(T).

J. Curiel, B. de Las Rivas, R. Muñoz, 2009, Protein expression and purification.

Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum

B. de Las Rivas, R. Muñoz, J. L. Lavín, 2018, Microbial Cell Factories.

Tannin Degradation by a Novel Tannase Enzyme Present in Some Lactobacillus plantarum Strains

B. de Las Rivas, R. Muñoz, J. Mancheño, 2014, Applied and Environmental Microbiology.

Characterization of the p-coumaric acid decarboxylase from Lactobacillus plantarum CECT 748(T).

J. Curiel, B. de Las Rivas, R. Muñoz, 2008, Journal of agricultural and food chemistry.

The Lp_3561 and Lp_3562 Enzymes Support a Functional Divergence Process in the Lipase/Esterase Toolkit from Lactobacillus plantarum

B. de Las Rivas, R. Muñoz, J. Mancheño, 2016, Front. Microbiol..

Tannin degradation by a novel tannase enzyme present in 3 some Lactobacillus plantarum strains 4 5 6 7

R. Muñoz, B. Rivas, J. Mancheño, 2014 .

The role of electrostatic interactions in the antitumor activity of dimeric RNases

J. Gavilanes, G. D'alessio, E. Notomista, 2006, The FEBS journal.

Enzymatic Synthesis of Therapeutic Nucleosides using a Highly Versatile Purine Nucleoside 2’‐DeoxyribosylTransferase from Trypanosoma brucei

F. Gago, J. Fernández‐Lucas, M. D. Blanco, 2018, ChemCatChem.

p‐Coumaric acid decarboxylase from Lactobacillus plantarum: Structural insights into the active site and decarboxylation catalytic mechanism

N. Campillo, B. de Las Rivas, R. Muñoz, 2010, Proteins.

Involvement of the amino‐terminal β‐hairpin of the Aspergillus ribotoxins on the interaction with membrances and nonspecific ribonuclease activity

N. Olmo, J. Gavilanes, J. Davies, 2001, Protein science : a publication of the Protein Society.

Characterization of the antifungal protein secreted by the mould Aspergillus giganteus.

J. Gavilanes, C. Vázquez, R. Campos-Olivas, 1995, Archives of biochemistry and biophysics.

Characterization of a natural larger form of the antifungal protein (AFP) from Aspergillus giganteus.

J. Gavilanes, J. Mancheño, A. Martínez-Ruíz, 1997, Biochimica et biophysica acta.

The Antifungal Protein AFP of Aspergillus giganteusIs an Oligonucleotide/Oligosaccharide Binding (OB) Fold-containing Protein That Produces Condensation of DNA*

N. Olmo, J. Gavilanes, J. Mancheño, 2002, The Journal of Biological Chemistry.

Sequence determination and molecular characterization of gigantin, a cytotoxic protein produced by the mould Aspergillus giganteus IFO 5818.

J. Gavilanes, J. Mancheño, A. Martínez-Ruíz, 1997, Archives of biochemistry and biophysics.

Directed, strong, and reversible immobilization of proteins tagged with a β-trefoil lectin domain: a simple method to immobilize biomolecules on plain agarose matrixes.

M. Lillo, F. López‐Gallego, J. Mancheño, 2012, Bioconjugate chemistry.

Characterization of a versatile arylesterase from Lactobacillus plantarum active on wine esters.

B. de Las Rivas, R. Muñoz, J. Mancheño, 2014, Journal of Agricultural and Food Chemistry.

Esterase LpEst1 from Lactobacillus plantarum: A Novel and Atypical Member of the αβ Hydrolase Superfamily of Enzymes

F. Gago, B. de Las Rivas, R. Muñoz, 2014, PloS one.

Characterization of a halotolerant lipase from the lactic acid 3 bacteria Lactobacillus plantarum useful in food fermentations 4 5 6

R. Muñoz, B. Rivas, J. Mancheño, 2014 .

Structure, biochemical characterization and analysis of the pleomorphism of carboxylesterase Cest‐2923 from Lactobacillus plantarum WCFS1

R. Muñoz, J. Mancheño, Rocío Benavente, 2013, The FEBS journal.

The crystal structure of galactitol‐1‐phosphate 5‐dehydrogenase from Escherichia coli K12 provides insights into its anomalous behavior on IMAC processes

B. de Las Rivas, R. Muñoz, G. Kohring, 2012, FEBS letters.

Structural Analysis of the Laetiporus sulphureus Hemolytic Pore-forming Lectin in Complex with Sugars*

I. Goldstein, J. Hermoso, J. Mancheño, 2005, Journal of Biological Chemistry.

Crystallization and preliminary X-ray diffraction studies of the water-soluble state of the pore-forming toxin sticholysin II from the sea anemone Stichodactyla helianthus.

J. Gavilanes, J. Hermoso, J. Mancheño, 2002, Acta crystallographica. Section D, Biological crystallography.

Laetiporus sulphureus lectin and aerolysin protein family.

I. Goldstein, D. Sher, J. Mancheño, 2010, Advances in experimental medicine and biology.

Hydralysins, a New Category of β-Pore-forming Toxins in Cnidaria*

D. Sher, E. Zlotkin, J. Mancheño, 2005, Journal of Biological Chemistry.

Hydralysins, a new category of beta-pore-forming toxins in cnidaria.

D. Sher, E. Zlotkin, J. Mancheño, 2005, The Journal of biological chemistry.

2′-Deoxyribosyltransferase from Leishmania mexicana, an efficient biocatalyst for one-pot, one-step synthesis of nucleosides from poorly soluble purine bases

F. Gago, J. Fernández‐Lucas, P. A. Sánchez‐Murcia, 2017, Applied Microbiology and Biotechnology.

High-resolution structural insights on the sugar-recognition and fusion tag properties of a versatile β-trefoil lectin domain from the mushroom Laetiporus sulphureus.

I. Goldstein, B. de Las Rivas, R. Muñoz, 2011, Glycobiology.

Membrane interaction of a beta-structure-forming synthetic peptide comprising the 116-139th sequence region of the cytotoxic protein alpha-sarcin.

M. Gasset, J. Gavilanes, J. Albar, 1995, Biophysical journal.

Predictive study of the conformation of the cytotoxic protein α-sarcin: a structural model to explain α-sarcin-membrane interaction

M. Gasset, J. Gavilanes, J. Mancheño, 1995 .

Translocation of alpha-sarcin across the lipid bilayer of asolectin vesicles.

J. Gavilanes, J. Mancheño, A. Martı́nez del Pozo, 1993, The Biochemical journal.

Identification of a Missing Link in the Evolution of an Enzyme into a Transcriptional Regulator

Eduardo Díaz, Gonzalo Durante-Rodríguez, Carlos Alfonso, 2013, PloS one.

Atomic crystal structure and sugar specificity of a β-trefoil lectin domain from the ectomycorrhizal basidiomycete Laccaria bicolor.

M. Menéndez, M. Lillo, J. Mancheño, 2023, International journal of biological macromolecules.

A peptide of nine amino acid residues from α‐sarcin cytotoxin is a membrane‐perturbing structure

J. Gavilanes, J. Albar, J. Mancheño, 2009 .

Deletion of the NH2-terminal β-Hairpin of the Ribotoxin α-Sarcin Produces a Nontoxic but Active Ribonuclease*

J. Gavilanes, M. Lizarbe, M. García-Mayoral, 2002, The Journal of Biological Chemistry.

Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin α‐sarcin

J. Gavilanes, J. Mancheño, A. Martínez-Ruíz, 2001 .

Assignment of the contribution of the tryptophan residues to the spectroscopic and functional properties of the ribotoxin α‐sarcin

J. Gavilanes, M. Bruix, J. Mancheño, 2000, Proteins.

Thermal unfolding of the cytotoxin alpha-sarcin: phospholipid binding induces destabilization of the protein structure.

J. Gavilanes, G. Fernández-Ballester, J. Mancheño, 1995, Biochimica et biophysica acta.

Spectroscopic characterization of the alkylated alpha-sarcin cytotoxin: analysis of the structural requirements for the protein-lipid bilayer hydrophobic interaction.

J. Gavilanes, J. Mancheño, A. Martı́nez del Pozo, 1995, Biochimica et biophysica acta.

Molecular Interactions Involved in the Passage of the Cytotoxic Protein α-Sarcin Across Membranes

M. Gasset, J. Gavilanes, J. Mancheño, 1994 .

Role of histidine‐50, glutamic acid‐96, and histidine‐137 in the ribonucleolytic mechanism of the ribotoxin α‐sarcin

J. Gavilanes, M. Bruix, J. Mancheño, 1999, Proteins.

The cytotoxin α‐sarcin behaves as a cyclizing ribonuclease

J. Gavilanes, J. Mancheño, A. Martínez-Ruíz, 1998 .

GSE4, a Small Dyskerin- and GSE24.2-Related Peptide, Induces Telomerase Activity, Cell Proliferation and Reduces DNA Damage, Oxidative Stress and Cell Senescence in Dyskerin Mutant Cells

R. Perona, L. Sastre, J. Mancheño, 2015, PloS one.

Crystal and electron microscopy structures of sticholysin II actinoporin reveal insights into the mechanism of membrane pore formation.

Juan A Hermoso, J. Gavilanes, J. Hermoso, 2003, Structure.

Insights into the activation of brain serine racemase by the multi‐PDZ domain glutamate receptor interacting protein, divalent cations and ATP

F. Baumgart, I. Rodrı́guez-Crespo, J. Mancheño, 2007, The FEBS journal.

Biochemical Characterization of the Transcriptional Regulator BzdR from Azoarcus sp. CIB*

J. García, G. Rivas, E. Díaz, 2010, The Journal of Biological Chemistry.

Refactoring the λ phage lytic/lysogenic decision with a synthetic regulator

E. Díaz, Gonzalo Durante-Rodríguez, M. Carmona, 2016, MicrobiologyOpen.

Overexpression, purification, crystallization and preliminary structural studies of catabolic ornithine transcarbamylase from Lactobacillus hilgardii.

B. de Las Rivas, R. Muñoz, I. Angulo, 2007, Acta crystallographica. Section F, Structural biology and crystallization communications.

Sticholysin II, a cytolysin from the sea anemone Stichodactyla helianthus, is a monomer‐tetramer associating protein

G. Rivas, J. Gavilanes, C. Alfonso, 1999, FEBS letters.

Secretion of recombinant pro- and mature fungal alpha-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: the Lys-Arg motif is required for maturation.

C. López-Otín, J. Gavilanes, J. Mancheño, 1998, Protein expression and purification.

Improving Properties of a Novel β-Galactosidase from Lactobacillus plantarum by Covalent Immobilization

J. Curiel, B. de Las Rivas, R. Muñoz, 2015, Molecules.

X-ray and Neutron Diffraction Approaches to the Structural Analysis of Protein-Lipid Interactions

J. Hermoso, J. Mancheño, E. Pebay‐Peyroula, 2006 .

Structural insights into the lipase/esterase behavior in the Candida rugosa lipases family: crystal structure of the lipase 2 isoenzyme at 1.97A resolution.

J. Hermoso, M. Martínez, B. Ochoa, 2003, Journal of molecular biology.

Preliminary X-ray analysis of twinned crystals of the Q88Y25_Lacpl esterase from Lactobacillus plantarum WCFS1.

B. de Las Rivas, R. Muñoz, J. Mancheño, 2011, Acta crystallographica. Section F, Structural biology and crystallization communications.

Overexpression, purification, crystallization and preliminary structural studies of p-coumaric acid decarboxylase from Lactobacillus plantarum.

B. de Las Rivas, R. Muñoz, J. Mancheño, 2007, Acta crystallographica. Section F, Structural biology and crystallization communications.

Crystallization of a proteolyzed form of the horse pancreatic lipase-related protein 2: structural basis for the specific detergent requirement.

J. Hermoso, J. Mancheño, S. Jayne, 2004, Acta crystallographica. Section D, Biological crystallography.

A structurally unique Fusobacterium nucleatum tannase provides detoxicant activity against gallotannins and pathogen resistance

S. Delgado, B. de Las Rivas, R. Muñoz, 2020, Microbial biotechnology.

Structural basis of the substrate specificity and instability in solution of a glycosidase from Lactobacillus plantarum.

B. de Las Rivas, R. Muñoz, J. Cumella, 2017, Biochimica et biophysica acta. Proteins and proteomics.

Substitution of histidine-137 by glutamine abolishes the catalytic activity of the ribosome-inactivating protein alpha-sarcin.

J. Gavilanes, J. Mancheño, A. Martínez-Ruíz, 1995, The Biochemical journal.

Overproduction in Escherichia coli and purification of the hemolytic protein sticholysin II from the sea anemone Stichodactyla helianthus.

J. Gavilanes, J. Mancheño, A. Martínez-Ruíz, 2000, Protein expression and purification.

Release of lipid vesicle contents by an antibacterial cecropin A-melittin hybrid peptide.

J. Gavilanes, D. Andreu, L. Rivas, 1996, Biochemistry.

Enantioselective oxidation of galactitol 1-phosphate by galactitol-1-phosphate 5-dehydrogenase from Escherichia coli.

F. Gago, B. de Las Rivas, R. Muñoz, 2015, Acta crystallographica. Section D, Biological crystallography.

Oriented Attachment of Recombinant Proteins to Agarose-Coated Magnetic Nanoparticles by Means of a β-Trefoil Lectin Domain.

M. Morales, Y. Luengo, J. Mancheño, 2016, Bioconjugate chemistry.

Phenotypic selection and characterization of randomly produced non‐haemolytic mutants of the toxic sea anemone protein sticholysin II

J. Gavilanes, J. Alegre-Cebollada, J. Mancheño, 2004, FEBS letters.

Crystal structure of the hexameric catabolic ornithine transcarbamylase from Lactobacillus hilgardii: Structural insights into the oligomeric assembly and metal binding.

B. de Las Rivas, R. Muñoz, G. Fox, 2009, Journal of molecular biology.

The pURI family of expression vectors: a versatile set of ligation independent cloning plasmids for producing recombinant His-fusion proteins.

J. Curiel, B. de Las Rivas, R. Muñoz, 2011, Protein expression and purification.

A new and promiscuous α/β hydrolase from Acinetobacter tandoii DSM 14970 T inactivates the mycotoxin ochratoxin A

B. de Las Rivas, J. Mancheño, M. C. Abeijón-Mukdsi, 2024, Applied Microbiology and Biotechnology.

Aspergillus niger Ochratoxinase Is a Highly Specific, Metal-Dependent Amidohydrolase Suitable for OTA Biodetoxification in Food and Feed

J. Mancheño, Laura Plaza-Vinuesa, Ana Sánchez-Arroyo, 2024, Journal of agricultural and food chemistry.

The salicylate 1,2-dioxygenase from Pseudaminobacter salicylatoxidans DSM 6986T is a bifunctional enzyme that inactivates the mycotoxin ochratoxin A by a novel amidohydrolase activity.

R. Muñoz, B. Rivas, J. Mancheño, 2023, International journal of biological macromolecules.

Deletion of the NH2-terminal beta-hairpin of the ribotoxin alpha-sarcin produces a nontoxic but active ribonuclease.

J. Gavilanes, M. Lizarbe, M. Bruix, 2002, The Journal of biological chemistry.