S. Chong

发表

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element.

F. Perler, Jack Benner, L. Vence, 1997, Gene.

Cysteine 265 Is in the Active Site of, But Is Not Essential for Catalysis by tRNA-Guanine Transglycosylase (TGT) from Escherichia coli

S. Chong, G. Garcia, 1997, Journal of protein chemistry.

Protein Synthesis Using a Reconstituted Cell‐Free System

S. Chong, H. Asahara, C. Tuckey, 2014, Current protocols in molecular biology.

Reconstitution of Protein Translation of Mycobacterium Reveals Functional Conservation and Divergence with the Gram-Negative Bacterium Escherichia coli

Q. Jin, Haiying Liu, Aashish Srivastava, 2016, PloS one.

Protein Splicing of the Saccharomyces cerevisiae VMA Intein without the Endonuclease Motifs*

Ming-Qun Xu, S. Chong, M. Q. Xu, 1997, The Journal of Biological Chemistry.

Fusions to self-splicing inteins for protein purification.

S. Chong, H. Paulus, M. Q. Xu, 2000, Methods in enzymology.

Engineered Cas12i2 is a versatile high-efficiency platform for therapeutic genome editing

David A. Scott, Lauren E. Alfonse, Winston X. Yan, 2022, Nature Communications.

Harnessing Inteins for Protein Purification and Characterization

Ming-Qun Xu, S. Chong, 2005 .

Intein-mediated Protein Purification

F. Perler, S. Chong, 2003 .

Single-process expression and purification of multiple recombinant proteins through cocultivation and affinity purification.

S. Chong, M. Porte, 2008, Analytical biochemistry.

Protein Splicing Involving the Saccharomyces cerevisiae VMA Intein

F. Perler, Jack Benner, S. Chong, 1996, The Journal of Biological Chemistry.

Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step.

Jack Benner, W. Liao, S. Chong, 1998, Nucleic acids research.

Cell-free protein synthesis of CRISPR ribonucleoproteins (RNP).

S. Chong, Colin Mcgaw, 2021, Methods in enzymology.

Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation.

T. C. Evans, Jack Benner, S. Chong, 1999, Gene.

Reconstitution of translation from Thermus thermophilus reveals a minimal set of components sufficient for protein synthesis at high temperatures and functional conservation of modern and ancient translation components

E. Gaucher, S. Chong, H. Asahara, 2012, Nucleic acids research.

One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil–water interfaces

Corey R Landry, D. Weitz, Ilke Akartuna, 2015, Proceedings of the National Academy of Sciences.

In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme

Haruichi Asahara, Shaorong Chong, S. Chong, 2010, Nucleic acids research.

Functionally diverse type V CRISPR-Cas systems

David A. Scott, Lauren E. Alfonse, E. Koonin, 2019, Science.

Cas13d is a compact RNA-targeting type VI CRISPR effector positively modulated by a WYL domain-containing accessory protein

Kira S. Makarova, Eugene V. Koonin, David A. Scott, 2018, Molecular cell.

Mass Spectrometry and Florescence Analysis of Snap-Nappa Arrays Expressed Using E. coli Cell_Free Expression System

Joshua LaBaer, Eugenia Pechkova, Claudio Nicolini, 2013 .

Overview of Cell‐Free Protein Synthesis: Historic Landmarks, Commercial Systems, and Expanding Applications

Shaorong Chong, S. Chong, 2014, Current protocols in molecular biology.

Quantifying elongation rhythm during full-length protein synthesis.

Jaskiran Kaur, Yale E Goldman, B. Cooperman, 2013, Journal of the American Chemical Society.

A mix-and-read drop-based in vitro two-hybrid method for screening high-affinity peptide binders

D. Weitz, S. Koehler, Yamei Cai, 2016, Scientific Reports.

Visualization of coupled protein folding and binding in bacteria and purification of the heterodimeric complex

Haoyong Wang, S. Chong, 2003, Proceedings of the National Academy of Sciences of the United States of America.

Intein-mediated protein purification of fusion proteins expressed under high-cell density conditions in E. coli.

S. Harcum, S. Chong, Shamik S. Sharma, 2006, Journal of biotechnology.

Simulation of large-scale production of a soluble recombinant protein expressed in Escherichia coli using an intein-mediated purification system

S. Harcum, S. Chong, Shamik S. Sharma, 2005, Applied biochemistry and biotechnology.

Modulation of Protein Splicing of the Saccharomyces cerevisiae Vacuolar Membrane ATPase Intein*

Ming-Qun Xu, S. Chong, C. Wotkowicz, 1998, The Journal of Biological Chemistry.

Productive interaction of chaperones with substrate protein domains allows correct folding of the downstream GFP domain.

S. Chong, E. Cantor, A. Zhang, 2005, Gene.

Rapid isolation of antigen-specific B-cells using droplet microfluidics

D. Weitz, L. Mazutis, J. Heyman, 2020, RSC advances.

A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based

J. Hillebrecht, Shaorong Chong, S. Chong, 2008, BMC biotechnology.

Study of Protein Splicing and Intein‐Mediated Peptide Bond Cleavage under High‐Cell‐Density Conditions

Haoyong Wang, S. Harcum, Shaorong Chong, 2003, Biotechnology progress.

Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins.

S. Chong, E. Cantor, A. Zhang, 2001, Gene.

Intein-mediated rapid purification of Cre recombinase.

S. Chong, E. Cantor, 2001, Protein expression and purification.

Proteins: Affinity Tags

S. Chong, 2001 .

Label-free single-cell protein quantification using a drop-based mix-and-read system

David A. Weitz, D. Weitz, J. Heyman, 2015, Scientific Reports.

Engineering Bacterial Transcription Regulation To Create a Synthetic in Vitro Two-Hybrid System for Protein Interaction Assays

James Inglese, James Inglese, P. Dranchak, 2014, Journal of the American Chemical Society.

Bottom-up single-molecule strategy for understanding subunit function of tetrameric β-galactosidase

D. Walt, S. Chong, Yu Jiang, 2018, Proceedings of the National Academy of Sciences.

Determining the lipid specificity of insoluble protein transmembrane domains.

L. R. Arriaga, S. Chong, R. Ziblat, 2018, Lab on a chip.