S. Tsunasawa

发表

Selective isolation of N-terminal peptides from proteins and their de novo sequencing by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry without regard to unblocking or blocking of N-terminal amino acids.

T. Nakazawa, O. Nishimura, H. Kuyama, 2008, Rapid communications in mass spectrometry : RCM.

Comparative amino acid sequence analysis of hemolysins produced by Vibrio hollisae and Vibrio parahaemolyticus

T. Honda, T. Miwatani, M. Yoh, 1989, Journal of bacteriology.

Novel Catalytic Mechanism of Nucleophilic Substitution by Asparagine Residue Involving Cyanoalanine Intermediate Revealed by Mass Spectrometric Monitoring of an Enzyme Reaction*

N. Esaki, T. Kurihara, S. Tsunasawa, 2000, The Journal of Biological Chemistry.

Identification of HS1 protein as a major substrate of protein-tyrosine kinase(s) upon B-cell antigen receptor-mediated signaling.

T. Yamamoto, T. Yamori, Y. Yamanashi, 1993, Proceedings of the National Academy of Sciences of the United States of America.

The primary structure of porcine liver acylamino acid-releasing enzyme deduced from cDNA sequences.

K. Asada, S. Tsunasawa, F. Sakiyama, 1989, Journal of biochemistry.

N‐Terminal protein modifications in an insect cell‐free protein synthesis system and their identification by mass spectrometry

O. Nishimura, T. Utsumi, Masaaki Ito, 2006, Proteomics.

Protein prenylation in an insect cell‐free protein synthesis system and identification of products by mass spectrometry

O. Nishimura, T. Utsumi, Masaaki Ito, 2007, Proteomics.

cDNA molecular cloning of Geotrichum candidum lipase.

Y. Shimada, Y. Tominaga, A. Sugihara, 1989, Journal of biochemistry.

Characterization of Geotrichum candidum lipase III with some preference for the inside ester bond of triglyceride

Y. Shimada, A. Sugihara, Y. Tominaga, 1993, Applied Microbiology and Biotechnology.

The specific isolation of C-terminal peptides of proteins through a transamination reaction and its advantage for introducing functional groups into the peptide.

Ei-ichi Matsuo, O. Nishimura, H. Kuyama, 2009, Rapid communications in mass spectrometry : RCM.

Cadherin cell adhesion molecules with distinct binding specificities share a common structure.

M. Takeichi, Y. Shirayoshi, S. Tsunasawa, 1986, The EMBO journal.

Human B-cell differentiation factor defined by an anti-peptide antibody and its possible role in autoantibody production.

T. Hirano, K. Yasukawa, K. Nakajima, 1987, Proceedings of the National Academy of Sciences of the United States of America.

N-terminal deblocking aminopeptidase (Pyrococcus furiosus)

S. Tsunasawa, 2004 .

High-resolution crystals of methionine aminopeptidase from Pyrococcus furiosus obtained by water-mediated transformation.

H. Oki, C. P. Libeu, T. Tsukihara, 1998, Journal of structural biology.

The single proline‐glutamine substitution at position 5 enhances the potency of amyloid fibril formation of murine apo A‐II

K. Higuchi, S. Tsunasawa, F. Sakiyama, 1986, FEBS letters.

Characterization of novel cholesterol esterase from Trichoderma sp. AS59 with high ability to synthesize steryl esters.

A. Sugihara, D. Nakayama, S. Tsunasawa, 2008, Journal of bioscience and bioengineering.

A method for terminus proteomics: selective isolation and labeling of N-terminal peptide from protein through transamination reaction.

Ei-ichi Matsuo, O. Nishimura, H. Kuyama, 2009, Bioorganic & medicinal chemistry letters.

Improved 2-nitrobenzenesulfenyl method: optimization of the protocol and improved enrichment for labeled peptides.

Ei-ichi Matsuo, O. Nishimura, E. Ando, 2006, Rapid communications in mass spectrometry : RCM.

A method for N-terminal de novo sequencing of Nα-blocked proteins by mass spectrometry.

T. Nakazawa, O. Nishimura, H. Kuyama, 2011, The Analyst.

Primary structure of nuclease P1 from Penicillium citrinum.

G. Dibó, K. Maekawa, S. Tsunasawa, 1991, European journal of biochemistry.

Micro-identification of amino-terminal acetylamino acids in proteins.

S. Tsunasawa, K. Narita, 1982, Journal of biochemistry.

Isocratic separation of PTH-amino acids at picomole level by reverse-phase HPLC in the presence of sodium dodecylsulfate.

J. Kondo, S. Tsunasawa, F. Sakiyama, 1985, Journal of biochemistry.

Reactivity of asparagine residue at the active site of the D105N mutant of fluoroacetate dehalogenase from Moraxella sp. B.

N. Esaki, T. Kurihara, S. Tsunasawa, 2004, Biochimica et biophysica acta.

dl-2-Haloacid Dehalogenase fromPseudomonas sp. 113 Is a New Class of Dehalogenase Catalyzing Hydrolytic Dehalogenation Not Involving Enzyme-Substrate Ester Intermediate*

M. Miyagi, K. Soda, V. Nardi-Dei, 1999, The Journal of Biological Chemistry.

Paracatalytic Inactivation of L-2-Haloacid Dehalogenase from Pseudomonas sp. YL by Hydroxylamine

M. Miyagi, K. Soda, N. Esaki, 1997, The Journal of Biological Chemistry.

[46] Acylamino acid-releasing enzyme from rat liver

S. Tsunasawa, K. Narita, 1976 .

Expression and activity of a gene encoding rat cytochrome c in the yeast Saccharomyces cerevisiae.

F. Sherman, J. Clements, S. Tsunasawa, 1989, Gene.

Reexamination of the primary structure of an antitumor protein, neocarzinostatin.

S. Tsunasawa, F. Sakiyama, H. Maeda, 1986, Archives of biochemistry and biophysics.

Characterization of superoxide dismutases purified from either anaerobically maintained or aerated Bacteroides gingivalis

S. Shizukuishi, A. Amano, S. Tsunasawa, 1990, Journal of bacteriology.

[14] Amino-terminal acetylation of proteins: An overview

S. Tsunasawa, F. Sakiyama, 1984 .

Preparation of N-Acylated Proteins Modified with Fatty Acids Having a Specific Chain Length Using an Insect Cell-Free Protein Synthesis System

O. Nishimura, T. Utsumi, Masaaki Ito, 2007, Bioscience, biotechnology, and biochemistry.

Complete amino acid sequence of bovine colostrum low‐M r cysteine proteinase inhibitor

M. Niinobe, S. Tsunasawa, F. Sakiyama, 1985, FEBS letters.

Bacterial 2-haloacid dehalogenases: structures and catalytic properties

M. Miyagi, K. Soda, V. Nardi-Dei, 1996 .

Two hevein homologs isolated from the seed of Pharbitis nil L. exhibit potent antifungal activity.

S. Y. Lee, M. Miyagi, K. Ha, 1998, Biochimica et biophysica acta.

Regulation of Human B Cell Differentiation: Molecular Structure and Immunological Functions of Human B Cell Differentiation Factor (BSF-2)

T. Hirano, R. Hardy, T. Matsuda, 1986 .

Expression of human Cu, Zn-superoxide dismutase in an insect cell-free system and its structural analysis by MALDI-TOF MS.

O. Nishimura, E. Ando, S. Tsunasawa, 2009, Journal of biotechnology.

Inhibition of Achromobacter protease I by lysinal derivatives.

T. Tanaka, S. Tsunasawa, F. Sakiyama, 1992, Bioscience, biotechnology, and biochemistry.

Transformation of yeast with synthetic oligonucleotides.

F. Sherman, S. Tsunasawa, R. Moerschell, 1988, Proceedings of the National Academy of Sciences of the United States of America.

Nucleotide sequence of the phoS gene, the structural gene for the phosphate-binding protein of Escherichia coli

H. Shinagawa, M. Amemura, J. Kondo, 1984, Journal of bacteriology.

A cysteine protease inhibitor stored in the large granules of horseshoe crab hemocytes: purification, characterization, cDNA cloning and tissue localization.

K. Agarwala, M. Miyagi, S. Kawabata, 1996, Journal of biochemistry.

The process of amyloid-like fibril formation by methionine aminopeptidase from a hyperthermophile, Pyrococcus furiosus.

K. Namba, Y. Yamagata, K. Yutani, 2000, Biochemistry.

Application of new deblocking aminopeptidase from Pyrococcus furiosis for microsequence analysis of blocked proteins.

H. Hirano, S. Tsunasawa, R. Kamp, 1998, Journal of protein chemistry.

Isolation and characterization of a cDNA coding for human myeloperoxidase.

K. Hashinaka, M. Yamada, S. Tsunasawa, 1987, Archives of biochemistry and biophysics.

Molecular structure of human lymphocyte receptor for immunoglobulin E

T. Hirano, T. Kaisho, R. Hardy, 1986, Cell.

High homology is present in the primary structures between murine senile amyloid protein (ASSAM) and human apolipoprotein A‐II

K. Higuchi, S. Tsunasawa, T. Takeda, 1986, FEBS letters.

A mutant hemolysin with lower biological activity produced by a mutant Vibrio parahaemolyticus.

T. Honda, T. Miwatani, S. Tsunasawa, 1989, FEMS microbiology letters.

Renotropic activity in ovine luteinizing hormone isoform(s).

S. Tsunasawa, F. Sakiyama, K. Shizume, 1988, Endocrinology.

Strategy for comprehensive identification of human N‐myristoylated proteins using an insect cell‐free protein synthesis system

Yoshinobu Ota, T. Utsumi, E. Ando, 2010, Proteomics.

The inhibitory properties and primary structure of a novel serine proteinase inhibitor from the fruiting body of the basidiomycete, Lentinus edodes.

M. Isemura, T. Koide, S. Tsunasawa, 1999, European journal of biochemistry.

The amino acid sequence of the amino terminal region of soybean trypsin inhibitor (Kunitz).

T. Koide, S. Tsunasawa, T. Ikenaka, 1971, Journal of Biochemistry (Tokyo).

Purification and characterization of a novel solvent-tolerant lipase from Fusarium heterosporum

Y. Shimada, A. Sugihara, Y. Tominaga, 1993 .

Purification and sequencing of a trypsin-sensitive cholecystokinin-releasing peptide from rat pancreatic juice. Its homology with pancreatic secretory trypsin inhibitor.

M. Hirose, K. Iwai, T. Fushiki, 1987, The Journal of biological chemistry.

Identification and characterization of genes and mutants for an N‐terminal acetyltransferase from yeast.

Susumu, M. Gribskov, M. Grunstein, 1989, The EMBO journal.

Purification and characterization of a novel thermostable lipase from Pseudomonas cepacia.

Y. Shimada, Y. Tominaga, A. Sugihara, 1992, Journal of biochemistry.

Overexpression and divalent metal binding properties of the methionyl aminopeptidase from Pyrococcus furiosus.

A. Copik, V. D'souza, R. Holz, 2002, Biochemistry.

Methionine aminopeptidase from the hyperthermophilic Archaeon Pyrococcus furiosus: molecular cloning and overexpression in Escherichia coli of the gene, and characteristics of the enzyme.

M. Miyagi, S. Tsunasawa, I. Kato, 1997, Journal of biochemistry.

Amino-terminal processing of mutant forms of yeast iso-1-cytochrome c. The specificities of methionine aminopeptidase and acetyltransferase.

J. Stewart, F. Sherman, S. Tsunasawa, 1985, The Journal of biological chemistry.

The primary structure of porcine aminoacylase 1 deduced from cDNA sequence.

S. Tsunasawa, F. Sakiyama, M. Mitta, 1992, Journal of biochemistry.

Studies of disulfide bond location in ovine lutropin β subunit

B. D. Burleigh, S. Tsunasawa, D. N. Ward, 1977 .

Purification, characterization and amino-acid sequencing of two pancreatic secretory trypsin inhibitors in rat pancreatic juice.

N. Yoshida, T. Mori, S. Tsunasawa, 1988, Biological chemistry Hoppe-Seyler.

Amino acid sequence of thermostable direct hemolysin produced by Vibrio parahaemolyticus.

A. Sugihara, T. Miwatani, S. Tsunasawa, 1987, Journal of biochemistry.

Purification and characterization of the spoOA protein of Bacillus subtilis from an overproducing strain of Escherichia coli.

T. Ikeuchi, S. Tsunasawa, F. Sakiyama, 1987, European journal of biochemistry.

The nucleotide sequence of human aminoacylase-1.

S. Tsunasawa, M. Mitta, I. Kato, 1993, Biochimica et biophysica acta.

Reaction Mechanism of Fluoroacetate Dehalogenase from Moraxella sp. B*

M. Miyagi, K. Soda, N. Esaki, 1998, The Journal of Biological Chemistry.

Microsequence analysis of the N‐terminally blocked proteins immobilized on polyvinylidene difluoride membrane by Western blotting

S. Komatsu, S. Tsunasawa, Y. Takagi, 1993, Electrophoresis.

A molecular-pathologic approach to murine senile amyloidosis. Serum precursor-apolipoprotein A-II variant (Pro5----Gln) presents only in the senile amyloidosis-prone SAM-P/1 and SAM-P/2 mice.

K. Higuchi, H. Naiki, S. Tsunasawa, 1987, Laboratory investigation; a journal of technical methods and pathology.

Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin

T. Taniguchi, T. Hirano, T. Matsuda, 1986, Nature.

The N-terminal residue of bovine rhodopsin is acetylmethionine.

S. Tsunasawa, H. Shichi, K. Narita, 1980, Biochimica et biophysica acta.

Methionine or not methionine at the beginning of a protein

F Sherman, J. Stewart, F. Sherman, 1985, BioEssays : news and reviews in molecular, cellular and developmental biology.

M. Asashima, A. Kurisaki, H. Kido, 2005, Biochemical and biophysical research communications.

The nucleotide sequence of human acylamino acid-releasing enzyme.

S. Tsunasawa, F. Sakiyama, M. Mitta, 1996, DNA research : an international journal for rapid publication of reports on genes and genomes.

A method for N-terminal de novo sequence analysis of proteins by matrix-assisted laser desorption/ionization mass spectrometry.

O. Nishimura, H. Kuyama, K. Sonomura, 2008, Analytical biochemistry.

Selective isolation of N-blocked peptide by combining AspN digestion, transamination, and tosylhydrazine glass treatment.

S. Futaki, Ei-ichi Matsuo, O. Nishimura, 2011, Analytical biochemistry.

Complete covalent structure of porcine liver acylamino acid-releasing enzyme and identification of its active site serine residue.

M. Miyagi, S. Tsunasawa, F. Sakiyama, 1995, Journal of biochemistry.

Purification and properties of acylamino acid-releasing enzyme from rat liver.

S. Tsunasawa, K. Narita, K. Ogata, 1975, Journal of biochemistry.

The amino acid sequence of soybean trypsin inhibitor (Kunitz).

T. Koide, S. Tsunasawa, T. Ikenaka, 1972, Journal of biochemistry.

Nigexine, a phospholipase A2 from cobra venom with cytotoxic properties not related to esterase activity. Purification, amino acid sequence, and biological properties.

A. Ménez, S. Tsunasawa, F. Sakiyama, 1989, The Journal of biological chemistry.

Properties of a hemolysin related to the thermostable direct hemolysin produced by a Kanagawa phenomenon negative, clinical isolate of Vibrio parahaemolyticus.

K. Goshima, T. Honda, T. Miwatani, 1990, Canadian journal of microbiology.

Antibodies against 70-kD heat shock cognate protein inhibit mediated nuclear import of karyophilic proteins

M. Miyagi, N. Imamoto, Y. Yoneda, 1992, The Journal of cell biology.

Overproduction of Thermus aquaticus DNA polymerase and its structural analysis by ion-spray mass spectrometry.

M. Miyagi, Y. Ishino, I. Kato, 1994, Journal of biochemistry.

Complete amino acid sequence of mouse prolactin.

S. Tsunasawa, F. Sakiyama, K. Kohmoto, 1984, European journal of biochemistry.

Multiple species of myeloperoxidase messenger RNAs produced by alternative splicing and differential polyadenylation.

K. Hashinaka, M. Yamada, S. Tsunasawa, 1988, Biochemistry.

The unusually slow unfolding rate causes the high stability of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus: equilibrium and kinetic studies of guanidine hydrochloride-induced unfolding and refolding.

K. Yutani, K. Ogasahara, M. Nakamura, 1998, Biochemistry.

Indication of possible post-translational formation of disulphide bonds in the beta-sheet domain of human lysozyme.

E. Kanaya, M. Kikuchi, K. Nokihara, 1993, The Biochemical journal.

Direct on-membrane peptide mass fingerprinting with MALDI-MS of tyrosine-phosphorylated proteins detected by immunostaining.

Eiji Ando, Masaru Furuta, Osamu Nishimura, 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Terminal proteomics: N‐ and C‐terminal analyses for high‐fidelity identification of proteins using MS

T. Nakazawa, O. Nishimura, T. Okamura, 2008, Proteomics.

On-membrane deblocking of proteins.

S. Komatsu, H. Hirano, S. Tsunasawa, 1997, Methods in molecular biology.

N-Terminal Acetylation of Mutationally Altered Form of Iso-1-Cytochromes c in Normal and nat1 - Strains Deficient in the Major N-Terminal Acetyl Transferase of the Yeast Saccharomyces Cerevisiae

R. Sternglanz, F. Sherman, S. Tsunasawa, 1993 .

Co- and Posttranslational Processes and Mitochondrial Import of Yeast Cytochrome c

R. Sternglanz, F. Sherman, M. Dumont, 1993 .

Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli

K. Makino, Y. Ishino, H. Shinagawa, 1986, Molecular and General Genetics MGG.

Microheterogeneity in the major phenobarbital-inducible forms of rabbit liver microsomal cytochrome P-450 as revealed by nucleotide sequencing of cloned cDNAs.

S. Tsunasawa, M. Komori, Y. Imai, 1988, Biochemistry.

Identification on membrane and characterization of phosphoproteins using an alkoxide-bridged dinuclear metal complex as a phosphate-binding tag molecule.

Eiji Ando, Masaru Furuta, Emiko Kinoshita-Kikuta, 2007, Journal of biomolecular techniques : JBT.

Structural homology between semidwarfism-related proteins and glutelin seed protein in rice (Oryza sativa L.)

S. Komatsu, H. Hirano, A. Nakamura, 1991, Theoretical and Applied Genetics.

Interisland Mutation of a Novel Phospholipase A2 from Trimeresurus flavoviridis Venom and Evolution of Crotalinae Group II Phospholipases A2

M. Ohno, T. Chijiwa, N. Oda-Ueda, 2003, Journal of Molecular Evolution.

NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae.

M. Miyagi, H Takakura, S Tsunasawa, 1992, The Journal of biological chemistry.

Complete amino acid sequence of a zinc metalloendoprotease from Streptomyces caespitosus.

N. Kasai, T. Kinoshita, S. Tsunasawa, 1995, European journal of biochemistry.

An improved method for de novo sequencing of arginine-containing, Nalpha-tris(2,4,6-trimethoxyphenyl)phosphonium-acetylated peptides.

O. Nishimura, H. Kuyama, K. Sonomura, 2008, Rapid communications in mass spectrometry : RCM.

A simple and highly successful C‐terminal sequence analysis of proteins by mass spectrometry

O. Nishimura, H. Kuyama, K. Sonomura, 2008, Proteomics.

Identification of mK1, a true tissue (glandular) kallikrein of mouse submandibular gland: tissue distribution and a comparison of kinin-releasing activity with other submandibular kallikreins.

S. Tsunasawa, K. Hosoi, F. Sakiyama, 1994, Journal of biochemistry.

The specificities of yeast methionine aminopeptidase and acetylation of amino-terminal methionine in vivo. Processing of altered iso-1-cytochromes c created by oligonucleotide transformation.

F Sherman, F. Sherman, Y. Hosokawa, 1990, The Journal of biological chemistry.

Synthesis and expression of genes encoding tuna, pigeon, and horse cytochromes c in the yeast Saccharomyces cerevisiae.

K. Jayaraman, S. A. Fingar, J. Shah, 1991, Gene.

A peptide that stimulates phosphorylation of the plant insulin-binding protein. Isolation, primary structure and cDNA cloning.

M. Miyagi, S. Komatsu, H. Hirano, 1994, European journal of biochemistry.

The amino acid sequence of S-antigen: N-terminus and uveitogenic peptides.

S. Tsunasawa, H. Shichi, 1989, Biochimica et biophysica acta.

Crystal structure of methionine aminopeptidase from hyperthermophile, Pyrococcus furiosus.

H. Oki, T. Tsukihara, T. Tahirov, 1998, Journal of molecular biology.

Pyrrolidone carboxyl peptidase from the hyperthermophilic Archaeon Pyrococcus furiosus: cloning and overexpression in Escherichia coli of the gene, and its application to protein sequence analysis.

S. Tsunasawa, S. Nakura, I. Kato, 1998, Journal of biochemistry.

Specific isolation of N-terminal fragments from proteins and their high-fidelity de novo sequencing.

T. Nakazawa, O. Nishimura, H. Kuyama, 2007, Rapid communications in mass spectrometry : RCM.

High sequence-coverage detection of proteolytic peptides using a bis(terpyridine)ruthenium(II) complex.

T. Nakazawa, Hitoshi Yamamoto, R. Masui, 2007, The Analyst.

Simultaneous detection of N-terminal fragment ions in a protein mixture using a ruthenium(II) complex.

T. Nakazawa, Hitoshi Yamamoto, R. Masui, 2007, Rapid Communications in Mass Spectrometry.

Selective detection of 2‐nitrobenzenesulfenyl‐labeled peptides by matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry using a novel matrix

Ei-ichi Matsuo, O. Nishimura, S. Izumi, 2006, Proteomics.

Studies on soybean trypsin inhibitors. 2. Amino-acid sequence around the reactive site of soybean trypsin inhibitor (Kunitz).

T. Koide, S. Tsunasawa, T. Ikenaka, 1973, European journal of biochemistry.

Identity of amino acid sequences of superoxide dismutase purified from both anaerobically maintained and aerated Porphyromonas gingivalis.

S. Shizukuishi, A. Amano, S. Tsunasawa, 1992, Oral microbiology and immunology.

[11] Micro-identification of amino-terminal acetylamino acids in proteins by using high-performance liquid chromatography

S. Tsunasawa, K. Narita, 1983 .

Amino acid sequence of nuclease S1 from Aspergillus oryzae.

A. Iwamatsu, G. Dibó, S. Tsunasawa, 1991, Journal of biochemistry.

Deblocking and subsequent microsequence analysis of N alpha-blocked proteins electroblotted onto PVDF membrane.

S. Komatsu, H. Hirano, S. Tsunasawa, 1992, Journal of Biochemistry (Tokyo).

Characterization and primary structure of a fatty acid-binding protein and its isoforms from the liver of the Amphibia, Rana catesbeiana.

K. Baba, S. Tsunasawa, S. Odani, 1999, Journal of biochemistry.

Deblocking and Subsequent Microsequence Analysis of N -Terminally Blocked Proteins Immobilized on PVDF Membrane

H. Hirano, S. Tsunasawa, 1993 .

Preparation of ubiquitin-conjugated proteins using an insect cell-free protein synthesis system.

O. Nishimura, T. Utsumi, E. Ando, 2010, Journal of biotechnology.

Amino acid sequence of copper,zinc-superoxide dismutase from spinach leaves.

K. Asada, Y. Katsube, N. Tanaka, 1986, Journal of biochemistry.

Amino-terminal acetylation of altered form of yeast iso-1-cytochromesc in normal andnat1− strains of yeast

R. Sternglanz, F. Sherman, S. Tsunasawa, 1992 .

Electrostatic stabilization in methionine aminopeptidase from hyperthermophile Pyrococcus furiosus.

K. Yutani, K. Ogasahara, S. Tsunasawa, 1998, Biochemistry.

Apparent dipeptidyl peptidase activities of acylamino acid-releasing enzymes.

T. Nakazawa, S. Tsunasawa, T. Imanaka, 1983, Journal of biochemistry.

Identification of the catalytic triad residues of porcine liver acylamino acid-releasing enzyme.

M. Miyagi, S. Tsunasawa, M. Mitta, 1998, Journal of biochemistry.

Distinction of Leu and Ile Using a Ruthenium(II) complex by MALDI-LIFT-TOF/TOF-MS analysis.

Hitoshi Yamamoto, R. Masui, S. Kuramitsu, 2005, Analytical chemistry.

Development of an insect cell-free system.

O. Nishimura, T. Utsumi, Masaaki Ito, 2010, Current Pharmaceutical Biotechnology.

Localization of the covalent C3b-binding site on C4b within the complement classical pathway C5 convertase, C4b2a3b.

H. Kozono, T. Kinoshita, J. Takeda, 1990, The Journal of biological chemistry.

Hydrolysis of S-2-aminoethylcysteinyl peptide bond by Achromobacter protease I.

S. Tsunasawa, F. Sakiyama, S. Kuwahara, 1994, Bioscience, biotechnology, and biochemistry.

Purification and application of a novel N-terminal deblocking aminopeptidase (DAP) from Pyrococcus furiosus.

S. Tsunasawa, 1998, Journal of Protein Chemistry.

Selective isolation of N-blocked peptide by combining AspN digestion, transamination, and tosylhydrazine glass treatment.

S. Futaki, Ei-ichi Matsuo, O. Nishimura, 2011, Analytical biochemistry.

Mass spectrometric analysis of the reactions catalyzed by l-2-haloacid dehalogenase mutants and implications for the roles of the catalytic amino acid residues

Masaru Miyagi, Tatsuo Kurihara, Nobuyoshi Esaki, 2003 .

Catalysis-linked inactivation of fluoroacetate dehalogenase by ammonia: a novel approach to probe the active-site environment.

M. Miyagi, N. Esaki, T. Kurihara, 2002, Journal of biochemistry.

Identification of the N-terminal residue of rhodopsin.

S. Tsunasawa, H. Shichi, K. Narita, 1982, Methods in enzymology.

Amino-terminally formylated tryptophan synthase alpha-subunit produced by the trp operon cloned in a plasmid vector.

K. Yutani, K. Ogasahara, Y. Sugino, 1980, Journal of Biochemistry (Tokyo).

Effect of single amino acid substitutions on the protease susceptibility of tryptophan synthase a subunit

K. Yutani, K. Ogasahara, Y. Sugino, 1985 .

Multiple gamma-glutamylation: a novel type of post-translational modification in a diapausing Artemia cyst protein.

T. Uchiumi, S. Tsunasawa, S. Odani, 2010, Biochemical and biophysical research communications.

Characterization of novel cholesterol esterase from Trichoderma sp. AS59 with high ability to synthesize steryl esters.

A. Sugihara, D. Nakayama, S. Tsunasawa, 2008, Journal of bioscience and bioengineering.

Lysine 190 is a covalent bilirubin-binding amino acid in human delta bilirubin

Y. Adachi, S. Tsunasawa, T. Kamisako, 1996 .

Acylamino acid-releasing enzyme from rat liver.

S. Tsunasawa, K. Narita, 1976, Methods in enzymology.

Expression of proteins containing disulfide bonds in an insect cell‐free system and confirmation of their arrangements by MALDI‐TOF MS

O. Nishimura, Masaaki Ito, E. Ando, 2007, Proteomics.

Posttranslational Modifications in an Insect Cell- Free Protein Synthesis System and Their Identification by MALDI-TOF MS

T. Utsumi, E. Ando, S. Tsunasawa, 2008 .