H. Dirr

发表

The Lyssavirus glycoprotein: A key to cross-immunity.

L. Martens, E. Vandermarliere, S. Buthelezi, 2016, Virology.

Structure determination and refinement of human alpha class glutathione transferase A1-1, and a comparison with the Mu and Pi class enzymes.

G J Kleywegt, G L Gilliland, R. Huber, 1993, Journal of molecular biology.

The effects of mutating Tyr9 and Arg15 on the structure, stability, conformational dynamics and mechanism of GSTA3-3.

Y. Sayed, S. Stoychev, H. Dirr, 2017, Biophysical chemistry.

Porcine class pi glutathione S-transferase: anionic ligand binding and conformational analysis.

H. Dirr, J. Erhardt, P. Bico, 1995, Biochimica et biophysica acta.

Recognition of the tripeptide glutathione by cytosolic glutathione S-transferases: An X-ray crystallographic analysis

R. Huber, P. Reinemer, H. Dirr, 1994 .

The kinetics of the substitution of coordinated H2O on Co(III) by cyanide in aquacobalamin (vitamin B12a) and in a corrole analogue

H. Marques, M. Fernandes, H. Dirr, 2015 .

Glutamate 85 and glutamate 228 contribute to the pH-response of the soluble form of chloride intracellular channel 1

M. Fernandes, H. Dirr, M. Cross, 2014, Molecular and Cellular Biochemistry.

Role of arginine 29 and glutamic acid 81 interactions in the conformational stability of human chloride intracellular channel 1.

S. Stoychev, M. Fernandes, H. Dirr, 2012, Biochemistry.

Plasmodium falciparum Hsp70-z, an Hsp110 homologue, exhibits independent chaperone activity and interacts with Hsp70-1 in a nucleotide-dependent fashion

H. Dirr, Ikechukwu Achilonu, A. Shonhai, 2016, Cell Stress and Chaperones.

Structural and biochemical characterization of Plasmodium falciparum Hsp70‐x reveals functional versatility of its C‐terminal EEVN motif

S. Makumire, H. Dirr, Ikechukwu Achilonu, 2018, Proteins.

Polymyxin B inhibits the chaperone activity of Plasmodium falciparum Hsp70

H. Dirr, O. Pooe, Ikechukwu Achilonu, 2017, Cell Stress and Chaperones.

A conserved interdomain interaction is a determinant of folding cooperativity in the GST fold.

N. Parbhoo, S. Stoychev, R. Adamson, 2011, Biochemistry.

The FOXP2 forkhead domain binds to a variety of DNA sequences with different rates and affinities

P. Machanick, S. Aron, L. Rotherham, 2017, Journal of biochemistry.

Refined crystal structure of porcine class Pi glutathione S-transferase (pGST P1-1) at 2.1 A resolution.

R. Huber, P. Reinemer, H. Dirr, 1994, Journal of molecular biology.

Mutational substitution of residues implicated by crystal structure in binding the substrate glutathione to human glutathione S-transferase pi.

R. Huber, P. Reinemer, W. E. Fahl, 1992, Journal of molecular biology.

An update on the biophysical character of the human eukaryotic elongation factor 1 beta: Perspectives from interaction with elongation factor 1 gamma

M. Papathanasopoulos, H. Dirr, Ikechukwu Achilonu, 2018, Journal of molecular recognition : JMR.

S-Nitrosation of Glutathione Transferase P1-1 Is Controlled by the Conformation of a Dynamic Active Site Helix*

L. Wallace, D. Balchin, H. Dirr, 2013, The Journal of Biological Chemistry.

Active-Site Mutations in the South African Human Immunodeficiency Virus Type 1 Subtype C Protease Have a Significant Impact on Clinical Inhibitor Binding: Kinetic and Thermodynamic Study

L. Morris, Y. Sayed, H. Dirr, 2008, Journal of Virology.

An empirical and theoretical description of Schistosoma japonicum glutathione transferase inhibition by bromosulfophthalein and indanyloxyacetic acid 94

Emmanuel A. Iwuchukwu, H. Dirr, Ikechukwu Achilonu, 2021 .

Characterization of the aflatoxin B1-binding site of rat albumin.

J. C. Schabort, H. Dirr, 1987, Biochimica et biophysica acta.

A Phosphomimetic Study Implicates Ser557 in Regulation of FOXP2 DNA Binding

H. Dirr, S. Fanucchi, Ashleigh Blane, 2018, The Protein Journal.

Conformational stability of Cys45‐alkylated and hydrogen peroxide‐oxidised glutathione S‐transferase

N. Sluis-Cremer, H. Dirr, 1995, FEBS letters.

The role of an evolutionarily conserved cis-proline in the thioredoxin-like domain of human class Alpha glutathione transferase A1-1.

L. Wallace, H. Dirr, J. Burke, 2003, The Biochemical journal.

Class-pi glutathione S-transferase is unable to regain its native conformation after oxidative inactivation by hydrogen peroxide.

N. Naidoo, N. Sluis-Cremer, H. Dirr, 1996, European journal of biochemistry.

Purification and characterisation of recombinant human eukaryotic elongation factor 1 gamma.

H. Dirr, Ikechukwu Achilonu, Thendo P Siganunu, 2014, Protein expression and purification.

S-Nitrosation destabilizes glutathione transferase P1-1.

D. Balchin, S. Stoychev, H. Dirr, 2013, Biochemistry.

Equilibrium unfolding of class pi glutathione S-transferase.

P. Reinemer, H. Dirr, 1991, Biochemical and biophysical research communications.

Purification and partial characterization of the glutathione S-transferase of rat erythrocytes.

J. C. Schabort, H. Dirr, 1988, Biochimica et biophysica acta.

The forkhead domain hinge-loop plays a pivotal role in DNA binding and transcriptional activity of FOXP2

S. Stoychev, H. Dirr, P. Naicker, 2018, Biological chemistry.

The in Vitro Phosphorylation of the Co-Chaperone mSTI1 by Cell Cycle Kinases Substantiates a Predicted Casein Kinase II-p34cdc2-NLS (CcN) Motif

G. Blatch, H. Dirr, M. Lässle, 2000, Biological chemistry.

Formation of an unfolding intermediate state of soluble chloride intracellular channel protein CLIC1 at acidic pH.

R. Adamson, H. Dirr, S. Fanucchi, 2008, Biochemistry.

Recombinant expression and purification of a functional bacterial metallo-chaperone PbrD-fusion construct as a potential biosorbent for Pb(II).

Keshav, H. Dirr, Ikechukwu Achilonu, 2019, Protein expression and purification.

The cochaperone murine stress-inducible protein 1: overexpression, purification, and characterization.

G. Blatch, M. Cheetham, J. van der Spuy, 2001, Protein expression and purification.

Heat shock cognate protein 70 chaperone-binding site in the co-chaperone murine stress-inducible protein 1 maps to within three consecutive tetratricopeptide repeat motifs.

G. Blatch, B. Kana, J. van der Spuy, 2000, The Biochemical journal.

Molecular recognition at the dimer interface of a class mu glutathione transferase: role of a hydrophobic interaction motif in dimer stability and protein function.

R. Armstrong, S. Codreanu, H. Dirr, 2002, Biochemistry.

Folding and assembly of dimeric human glutathione transferase A1-1.

L. Wallace, H. Dirr, 1999, Biochemistry.

Equilibrium folding of dimeric class mu glutathione transferases involves a stable monomeric intermediate.

L. Wallace, J. Luo, R. Armstrong, 2000, Biochemistry.

Structural dynamics of soluble chloride intracellular channel protein CLIC1 examined by amide hydrogen-deuterium exchange mass spectrometry.

Virgil L. Woods, Sheng Li, S. Stoychev, 2009, Biochemistry.

Glutamate 85 and glutamate 228 contribute to the pH-response of the soluble form of chloride intracellular channel 1

M. Fernandes, H. Dirr, M. Cross, 2014, Molecular and Cellular Biochemistry.

A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis

B. Pletschke, R. Krause, K. Rashamuse, 2020, Molecules.

Effect of aflatoxin B1 on human platelet protein kinase C.

H. Dirr, L. van den Heever, 1991, The International journal of biochemistry.

Probing the nature of the Co(III) ion in corrins: a comparison of the thermodynamics and kinetics of the ligand substitution reactions of aquacyanocobyrinic acid heptamethyl ester and stable yellow aquacyanocobyrinic acid heptamethyl ester.

H. Marques, H. Dirr, S. M. Chemaly, 2011, Inorganic chemistry.

X-ray crystal structures of cytosolic glutathione S-transferases. Implications for protein architecture, substrate recognition and catalytic function.

R. Huber, P. Reinemer, H. Dirr, 1994, European journal of biochemistry.

A Lys-Trp cation-π interaction mediates the dimerization and function of the chloride intracellular channel protein 1 transmembrane domain.

H. Dirr, A. Polyansky, S. Fanucchi, 2014, Biochemistry.

Aflatoxin B1 transport in rat blood plasma. Binding to albumin in vivo and in vitro and spectrofluorimetric studies into the nature of the interaction.

J. C. Schabort, H. Dirr, 1986, Biochimica et biophysica acta.

Overexpression, Purification and Functional Characterisation of Wild-Type HIV-1 Subtype C Protease and Two Variants Using a Thioredoxin and His-Tag Protein Fusion System

H. Dirr, Ikechukwu Achilonu, Yasien Sayed, 2018, The Protein Journal.

The study of degradation mechanisms of glyco-engineered plant produced anti-rabies monoclonal antibodies E559 and 62-71-3

L. Martens, E. Vandermarliere, S. Buthelezi, 2018, PloS one.

Polymorphic Diversity: N-Phenylbenzamide as a Possible Polymorphophore

H. Marques, M. Fernandes, H. Dirr, 2013 .

High yield purification of JNK1β1 and activation by in vitro reconstitution of the MEKK1→MKK4→JNK MAPK phosphorylation cascade.

H. Dirr, Ikechukwu Achilonu, Gavin R. Owen, 2013, Protein expression and purification.

The three‐dimensional structure of class pi glutathione S‐transferase in complex with glutathione sulfonate at 2.3 A resolution.

R. Huber, P. Reinemer, R. Ladenstein, 1991, The EMBO journal.

Molecular basis of inhibition of Schistosoma japonicum glutathione transferase by ellagic acid: insights into biophysical and structural studies.

H. Dirr, R. Pandian, Ikechukwu Achilonu, 2020, Molecular and biochemical parasitology.

Native dimer stabilizes the subunit tertiary structure of porcine class pi glutathione S-transferase.

H. Dirr, J. Erhardt, 1995, European journal of biochemistry.

V2-Directed Vaccine-like Antibodies from HIV-1 Infection Identify an Additional K169-Binding Light Chain Motif with Broad ADCC Activity

B. Haynes, S. Karim, L. Morris, 2018, Cell reports.

Phosphorylation- and nucleotide-binding-induced changes to the stability and hydrogen exchange patterns of JNK1β1 provide insight into its mechanisms of activation.

S. Stoychev, H. Dirr, Ikechukwu Achilonu, 2014, Journal of molecular biology.

Purification and properties of an esterase from Cucurbita maxima fruit tissue

J. C. Schabort, A. Nourse, I. Dubery, 1989 .

Electrostatic interactions affecting the active site of class sigma glutathione S-transferase.

R. Armstrong, H. Dirr, J. Stevens, 2000, The Biochemical journal.

Overexpression, Purification and Characterisation of the Plasmodium falciparum Hsp70-z (PfHsp70-z) Protein

H. Dirr, Ikechukwu Achilonu, A. Shonhai, 2015, PloS one.

Three-dimensional structure of class pi glutathione S-transferase from human placenta in complex with S-hexylglutathione at 2.8 A resolution.

M W Parker, R. Huber, P. Reinemer, 1992, Journal of molecular biology.

Solvent effects on the spectroscopic properties of aflatoxin B1.

H. Dirr, 1987, The International journal of biochemistry.

Protease inhibition in African subtypes of HIV-1.

A. Velázquez‐Campoy, E. Freire, S. Vega, 2003, AIDS reviews.

A conserved cation binding site in the DNA binding domain of forkhead box transcription factors regulates DNA binding by FOXP2.

H. Dirr, S. Fanucchi, G. Morris, 2018, Archives of biochemistry and biophysics.

[22] X-ray structure methods for glutathione binding

R. Huber, P. Reinemer, H. Dirr, 1995 .

A conserved cationic motif enhances membrane binding and insertion of the chloride intracellular channel protein 1 transmembrane domain

H. Dirr, S. Fanucchi, Bradley Peter, 2014, European Biophysics Journal.

JNK1β1 is phosphorylated during expression in E. coli and in vitro by MKK4 at three identical novel sites.

S. Stoychev, H. Dirr, Ikechukwu Achilonu, 2013, Biochemical and biophysical research communications.

A Single Amino Acid in the Hinge Loop Region of the FOXP Forkhead Domain is Significant for Dimerisation

K. Perumal, H. Dirr, S. Fanucchi, 2015, The Protein Journal.

(−)-Epigallocatechin-3-Gallate Inhibits the Chaperone Activity of Plasmodium falciparum Hsp70 Chaperones and Abrogates Their Association with Functional Partners

S. Makumire, H. Dirr, A. Shonhai, 2017, Molecules.

Domain-domain interface packing at conserved Trp-20 in class alpha glutathione transferase impacts on protein stability.

L. Wallace, H. Dirr, J. Burke, 2000, Biochimica et biophysica acta.

Class sigma glutathione transferase unfolds via a dimeric and a monomeric intermediate: impact of subunit interface on conformational stability in the superfamily.

R. Armstrong, H. Dirr, J. Stevens, 1998, Biochemistry.

F99 is Critical for Dimerization and Activation of South African HIV-1 Subtype C Protease

H. Dirr, P. Naicker, P. Seele, 2013, The Protein Journal.

Molecular dynamics and ligand docking of a hinge region variant of South African HIV-1 subtype C protease.

H. Dirr, Ikechukwu Achilonu, Vijayakumar Balakrishnan, 2018, Journal of molecular graphics & modelling.

A Conserved N-capping Motif Contributes Significantly to the Stabilization and Dynamics of the C-terminal Region of Class Alpha Glutathione S-Transferases*

H. Dirr, Yasien Sayed, D. Kuhnert, 2005, Journal of Biological Chemistry.

Conformational stability of pGEX‐expressed Schistosoma japonicum glutathione S‐transferase: A detoxification enzyme and fusion‐protein affinity tag

H. Klump, N. Sluis-Cremer, H. Dirr, 1997, Protein science : a publication of the Protein Society.

Drug Susceptibility and Replication Capacity of a Rare HIV-1 Subtype C Protease Hinge Region Variant

L. Morris, A. Basson, H. Dirr, 2019, Antiviral therapy.

A topologically conserved aliphatic residue in alpha-helix 6 stabilizes the hydrophobic core in domain II of glutathione transferases and is a structural determinant for the unfolding pathway.

L. Wallace, G. Blatch, H. Dirr, 1998, The Biochemical journal.

Effect of glutathione, glutathione sulphonate and S‐hexylglutathione on the conformational stability of class pi glutathione S‐transferase

H. Dirr, J. Erhardt, 1996, FEBS letters.

Arginine 15 stabilizes an S(N)Ar reaction transition state and the binding of anionic ligands at the active site of human glutathione transferase A1-1.

T. Sewell, S. Pelly, H. Dirr, 2010, Biophysical chemistry.

Role of individual histidines in the pH-dependent global stability of human chloride intracellular channel 1.

M. Fernandes, H. Dirr, Ikechukwu Achilonu, 2012, Biochemistry.

The Isomerization of Δ5-Androstene-3,17-dione by the Human Glutathione Transferase A3-3 Proceeds via a Conjugated Heteroannular Diene Intermediate*

H. Dirr, Ikechukwu Achilonu, J. L. Daka, 2014, The Journal of Biological Chemistry.

Determination of a binding site for a non-substrate ligand in mammalian cytosolic glutathione S-transferases by means of fluorescence-resonance energy transfer.

N. Naidoo, T. Manoharan, N. Sluis-Cremer, 1996, European journal of biochemistry.

Class π glutathione S-transferase from pig lung

R. Huber, P. Reinemer, R. Ladenstein, 1991 .

Stability and unfolding of reduced Escherichia coli glutaredoxin 2: a monomeric structural homologue of the glutathione transferase family.

L. Wallace, H. Dirr, S. Gildenhuys, 2008, Biochemistry.

The intersubunit lock-and-key motif in human glutathione transferase A1-1: role of the key residues Met51 and Phe52 in function and dimer stability.

H. Dirr, Carla S. Alves, Yasien Sayed, 2006, The Biochemical journal.

The hydrophobic lock‐and‐key intersubunit motif of glutathione transferase A1‐1: implications for catalysis, ligandin function and stability

L. Wallace, H. Dirr, Yasien Sayed, 2000, FEBS letters.

Membrane mimetics induce helix formation and oligomerization of the chloride intracellular channel protein 1 transmembrane domain.

H. Dirr, S. Fanucchi, Bradley Peter, 2013, Biochemistry.

Tertiary interactions stabilise the C-terminal region of human glutathione transferase A1-1: a crystallographic and calorimetric study.

T. Sewell, H. Dirr, Salerwe Mosebi, 2005, Journal of molecular biology.

Amide hydrogen exchange in HIV–1 subtype B and C proteases – insights into reduced drug susceptibility and dimer stability

S. Stoychev, H. Dirr, P. Naicker, 2014, The FEBS journal.

Role of the C-terminal helix 9 in the stability and ligandin function of class alpha glutathione transferase A1-1.

L. Wallace, H. Dirr, 1999, Biochemistry.

Stability of the domain interface contributes towards the catalytic function at the H-site of class alpha glutathione transferase A1-1.

D. Balchin, R. Adamson, M. Fernandes, 2010, Biochimica et biophysica acta.

Equilibrium and kinetic unfolding properties of dimeric human glutathione transferase A1-1.

L. Wallace, N. Sluis-Cremer, H. Dirr, 1998, Biochemistry.

Nuclear localisation sequences of chloride intracellular channels 1 and 4 facilitate nuclear import via interactions with import mediator importin‐α: An empirical and theoretical perspective

H. Dirr, Ikechukwu Achilonu, R. Worth, 2022, Journal of molecular recognition : JMR.

Structural insights into the South African HIV-1 subtype C protease: impact of hinge region dynamics and flap flexibility in drug resistance

M. Soliman, Mahmoud A. A. Ibrahim, M. Fernandes, 2013, Journal of biomolecular structure & dynamics.

Class Pi glutathione transferase unfolds via a dimeric and not monomeric intermediate: functional implications for an unstable monomer.

L. Wallace, D. Balchin, H. Dirr, 2010, Biochemistry.

Residue 219 impacts on the dynamics of the C-terminal region in glutathione transferase A1-1: implications for stability and catalytic and ligandin functions.

H. Dirr, J. Burke, Salerwe Mosebi, 2003, Biochemistry.

Energetics of Glutathione Binding to Human Eukaryotic Elongation Factor 1 Gamma: Isothermal Titration Calorimetry and Molecular Dynamics Studies

M. Tomescu, H. Dirr, Ikechukwu Achilonu, 2016, The Protein Journal.

Energetics of ligand binding to human glutathione transferase A1-1: Tyr-9 associated localisation of the C-terminal helix is ligand-dependent.

D. Balchin, H. Dirr, Yasien Sayed, 2011, Biophysical chemistry.

Characterization of the binding of 8-anilinonaphthalene sulfonate to rat class Mu GST M1-1.

R. Armstrong, H. Dirr, Salerwe Mosebi, 2008, Biophysical chemistry.

Analysis of the levels of conservation of the J domain among the various types of DnaJ-like proteins

G. Blatch, M. Cheetham, M E Cheetham, 2000, Cell stress & chaperones.

Characterization of bromosulphophthalein binding to human glutathione S-transferase A1-1: thermodynamics and inhibition kinetics.

H. Dirr, Yasien Sayed, Doris Kolobe, 2004, The Biochemical journal.

Thermodynamics of the ligandin function of human class Alpha glutathione transferase A1-1: energetics of organic anion ligand binding.

H. Dirr, J. Hornby, Yasien Sayed, 2002, The Biochemical journal.

The role of a topologically conserved isoleucine in glutathione transferase structure, stability and function.

B. Sewell, M. Fernandes, H. Dirr, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Effect of macromolecular crowding on the stability of monomeric glutaredoxin 2 and dimeric glutathione transferase A1-1

H. Dirr, S. Gildenhuys, D. Kuhnert, 2008 .

Double trouble? Gag in conjunction with double insert in HIV protease contributes to reduced DRV susceptibility.

L. Morris, A. Basson, H. Dirr, 2019, The Biochemical journal.

Impact of domain interchange on conformational stability and equilibrium folding of chimeric class μ glutathione transferases

L. Wallace, R. Armstrong, H. Dirr, 2002, Protein science : a publication of the Protein Society.

Structure determination and refinement of human alpha class glutathione transferase A1-1, and a comparison with the Mu and Pi class enzymes.

P. Reinemer, G. Kleywegt, G. Gilliland, 1993, Journal of molecular biology.

Double mutation at the subunit interface of glutathione transferase rGSTM1-1 results in a stable, folded monomer.

R. Armstrong, Lawrence C. Thompson, H. Dirr, 2006, Biochemistry.

Accessibility of the tryptophan residues and flavin prosthetic group of β-cyclopiazonate oxidocyclase to solvent studied by fluorescence quenching

J. C. Schabort, H. Dirr, G. L. Erasmus, 1988 .