K. Niefind

发表

Structural basis for signaling by exclusive EDS1 heteromeric complexes with SAG101 or PAD4 in plant innate immunity.

R. Guérois, J. Parker, Stephan Wagner, 2013, Cell host & microbe.

Design of CK2β‐Mimicking Peptides as Tools To Study the CK2α/CK2β Interaction in Cancer Cells

C. Götz, M. Pietsch, K. Niefind, 2019, ChemMedChem.

Crystal structure and snapshots along the reaction pathway of a family 51 α‐L‐arabinofuranosidase

Dietmar Schomburg, D. Schomburg, G. Shoham, 2003 .

The interaction of CK2α and CK2β, the subunits of protein kinase CK2, requires CK2β in a preformed conformation and is enthalpically driven

O. Issinger, E. Brunstein, K. Niefind, 2008, Protein science : a publication of the Protein Society.

Inter- and Supramolecular Interactions of Protein Kinase CK2 and their Relevance for Genome Integrity

O. Issinger, B. Olsen, K. Niefind, 2005 .

Crystal structure of creatininase from Pseudomonas putida: a novel fold and a case of convergent evolution.

D. Schomburg, B. Beuth, K. Niefind, 2003, Journal of molecular biology.

Unexpected CK2 beta-antagonistic functionality of bisubstrate inhibitors targeting protein kinase CK2.

J. Jose, M. Pietsch, K. Niefind, 2020 .

Unexpected CK2β-antagonistic functionality of bisubstrate inhibitors targeting protein kinase CK2.

J. Jose, M. Pietsch, K. Niefind, 2020, Bioorganic chemistry.

A Note of Caution on the Role of Halogen Bonds for Protein Kinase/Inhibitor Recognition Suggested by High- And Low-Salt CK2α Complex Structures.

O. Issinger, S. Yarmoluk, B. Guerra, 2015, ACS chemical biology.

Structural Bases of Protein Kinase CK2 Function and Inhibition

R. Battistutta, K. Niefind, 2013 .

Protein Kinase CK2 in Health and Disease

O. Issinger, K. Niefind, J. Raaf, 2009, Cellular and Molecular Life Sciences.

Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.

J. Parker, K. Niefind, C. Toelzer, 2019, Journal of structural biology.

Atomic resolution structures of R-specific alcohol dehydrogenase from Lactobacillus brevis provide the structural bases of its substrate and cosubstrate specificity.

Dietmar Schomburg, D. Schomburg, Jörg P. Müller, 2005, Journal of molecular biology.

Crystallization and preliminary characterization of crystals of R-alcohol dehydrogenase from Lactobacillus brevis.

D. Schomburg, W. Hummel, K. Niefind, 2000, Acta crystallographica. Section D, Biological crystallography.

Crystallization and preliminary crystallographic analysis of Gre2p, an NADP(+)-dependent alcohol dehydrogenase from Saccharomyces cerevisiae.

W. Hummel, K. Niefind, Marion Müller, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Enzyme-substrate complexes of the quinate/shikimate dehydrogenase from Corynebacterium glutamicum enable new insights in substrate and cofactor binding, specificity, and discrimination

D. Schomburg, K. Niefind, A. Höppner, 2013, Biological chemistry.

Crystallization and preliminary X-ray analysis of a family 51 glycoside hydrolase, the alpha-l-arabinofuranosidase from Geobacillus stearothermophilus T-6.

D. Schomburg, G. Shoham, Y. Shoham, 2003, Acta crystallographica. Section D, Biological crystallography.

Protein kinase CK2: from structures to insights

O. Issinger, K. Niefind, J. Raaf, 2009, Cellular and Molecular Life Sciences.

De novo variants of CSNK2B cause a new intellectual disability-craniodigital syndrome by disrupting the canonical Wnt signaling pathway

Tzung-Chien Hsieh, N. Fleischer, P. Krawitz, 2022, HGG advances.

Biological properties and structural study of new aminoalkyl derivatives of benzimidazole and benzotriazole, dual inhibitors of CK2 and PIM1 kinases.

K. Niefind, M. Wielechowska, M. Bretner, 2018, Bioorganic chemistry.

Exploring the intramolecular phosphorylation sites in human Chk2.

M. Larsen, O. Issinger, B. Boldyreff, 2008, Mutation research.

The ‘regulatory’ β-subunit of protein kinase CK2 negatively influences p53-mediated allosteric effects on Chk2 activation

L. Wiesmüller, D. Meek, O. Issinger, 2005, Oncogene.

Structural and mechanistic basis of the inhibitory potency of selected 2-aminothiazole compounds on protein kinase CK2.

J. Jose, K. Niefind, D. Lindenblatt, 2020, Journal of medicinal chemistry.

First inactive conformation of CK 2 , the catalytic subunit of protein kinase CK 2

O. Issinger, K. Niefind, J. Raaf, 2018 .

Development of a high-throughput screening-compatible assay to identify inhibitors of the CK2α/CK2β interaction.

C. Götz, O. Issinger, V. Helms, 2015, Analytical biochemistry.

Biochemical characterization of CK2α and α′ paralogues and their derived holoenzymes: evidence for the existence of a heterotrimeric CK2α′-holoenzyme forming trimeric complexes

O. Issinger, B. Olsen, K. Niefind, 2008, Molecular and Cellular Biochemistry.

Crystallization and preliminary crystallographic analysis of Arabidopsis thaliana EDS1, a key component of plant immunity, in complex with its signalling partner SAG101.

J. Parker, Stephan Wagner, Steffen Rietz, 2011, Acta crystallographica. Section F, Structural biology and crystallization communications.

Crystallization and preliminary X-ray analysis of a bacterial L-amino-acid oxidase from Rhodococcus opacus.

D. Schomburg, W. Hummel, B. Geueke, 2006, Acta crystallographica. Section F, Structural biology and crystallization communications.

Crystallization and preliminary crystallographic analysis of a family 43 beta-D-xylosidase from Geobacillus stearothermophilus T-6.

D. Schomburg, G. Shoham, Y. Shoham, 2005, Acta crystallographica. Section F, Structural biology and crystallization communications.

Crystallization and preliminary crystallographic analysis of a flavoprotein NADH oxidase from Lactobacillus brevis.

D. Schomburg, W. Hummel, K. Niefind, 2005, Acta crystallographica. Section F, Structural biology and crystallization communications.

Crystallization and preliminary crystallographic analysis of creatininase from Pseudomonas putida.

D. Schomburg, B. Beuth, K. Niefind, 2002, Acta crystallographica. Section D, Biological crystallography.

Crystallization, preliminary X-ray analysis of a native and selenomethionine D-hydantoinase from Thermus sp.

D. Schomburg, K. Niefind, J. Abendroth, 2000, Acta crystallographica. Section D, Biological crystallography.

Expression, purification and crystallization of the catalytic subunit of protein kinase CK2 from Zea mays.

D. Schomburg, O. Issinger, L. Pinna, 1998, Acta crystallographica. Section D, Biological crystallography.

and their derived holoenzymes: evidence for the existence of a heterotrimeric CK2a 0 -holoenzyme forming trimeric complexes

O. Issinger, B. Olsen, K. Niefind, 2008 .

P 5 Calpain-mediated activation of cyclin dependent kinase 5 is critical for alpha-synuclein toxicity in vitro

O. Issinger, Lawrence W. Miller, K. Niefind, 2012 .

Structural basis of the constitutive activity of protein kinase CK2.

O. Issinger, B. Guerra, B. Olsen, 2010, Methods in enzymology.

X-ray structure of a dihydropyrimidinase from Thermus sp. at 1.3 A resolution.

Dietmar Schomburg, Jan Abendroth, D. Schomburg, 2002, Journal of molecular biology.

Crystal structure of D-hydantoinase from Bacillus stearothermophilus: insight into the stereochemistry of enantioselectivity.

D. Schomburg, Hak-Sung Kim, Jimin Wang, 2002, Biochemistry.

Unexpected Binding Mode of a Potent Indeno[1,2-b]indole-Type Inhibitor of Protein Kinase CK2 Revealed by Complex Structures with the Catalytic Subunit CK2α and Its Paralog CK2α′

J. Jose, K. Niefind, D. Lindenblatt, 2017, Pharmaceuticals.

D Schomburg, D. Schomburg, K. Niefind, 1991, Journal of molecular biology.

Expanding the Application Range of Microbial Oxidoreductases by an Alcohol Dehydrogenase from Comamonas testosteroni with a Broad Substrate Spectrum and pH Profile

H. Gröger, W. Hummel, K. Niefind, 2020, Catalysts.

The crystal structure of R-specific alcohol dehydrogenase from Lactobacillus brevis suggests the structural basis of its metal dependency.

D. Schomburg, Jörg P. Müller, W. Hummel, 2003, Journal of molecular biology.

Expression, purification, and aggregation studies of His-tagged thermoalkalophilic lipase from Bacillus thermocatenulatus.

D. Schomburg, K. Niefind, Nils Helge Schlieben, 2004, Protein expression and purification.

Crystal structure of a ternary complex of D-2-hydroxyisocaproate dehydrogenase from Lactobacillus casei, NAD+ and 2-oxoisocaproate at 1.9 A resolution.

D. Schomburg, M. Kiess, U. Dengler, 1997, Journal of molecular biology.

The structure of a bacterial L-amino acid oxidase from Rhodococcus opacus gives new evidence for the hydride mechanism for dehydrogenation.

D. Schomburg, W. Hummel, K. Niefind, 2007, Journal of molecular biology.

Purification and characterization of the CK2α'-based holoenzyme, an isozyme of CK2α : A comparative analysis

O. Issinger, B. Boldyreff, B. Olsen, 2006 .

Primary and secondary interactions between CK2α and CK2β lead to ring-like structures in the crystals of the CK2 holoenzyme

O. Issinger, K. Niefind, 2005, Molecular and Cellular Biochemistry.

A π-Halogen Bond of Dibenzofuranones with the Gatekeeper Phe113 in Human Protein Kinase CK2 Leads to Potent Tight Binding Inhibitors

C. Götz, B. Wünsch, J. Jose, 2018, Pharmaceuticals.

An EDS1 EP-domain surface mediating timely transcriptional reprogramming of immunity genes

Barbara Kracher, J. Parker, Patrick von Born, 2018, bioRxiv.

Crystal structure of human protein kinase CK2: insights into basic properties of the CK2 holoenzyme

O. Issinger, B. Guerra, K. Niefind, 2001, The EMBO journal.

Crystal structure of a C-terminal deletion mutant of human protein kinase CK2 catalytic subunit.

O. Issinger, B. Boldyreff, K. Niefind, 2003, Journal of molecular biology.

The CK2 alpha/CK2 beta interface of human protein kinase CK2 harbors a binding pocket for small molecules.

O. Issinger, E. Brunstein, K. Niefind, 2008, Chemistry & biology.

Molecular Plasticity of Crystalline CK2α' Leads to KN2, a Bivalent Inhibitor of Protein Kinase CK2 with Extraordinary Selectivity.

C. Götz, J. Jose, K. Niefind, 2021, Journal of medicinal chemistry.

Diacritic Binding of an Indenoindole Inhibitor by CK2α Paralogs Explored by a Reliable Path to Atomic Resolution CK2α′ Structures

J. Jose, K. Niefind, D. Lindenblatt, 2019, ACS omega.

First structure of protein kinase CK2 catalytic subunit with an effective CK2β-competitive ligand.

O. Issinger, B. Guerra, J. Jose, 2013, ACS chemical biology.

The catalytic subunit of human protein kinase CK2 structurally deviates from its maize homologue in complex with the nucleotide competitive inhibitor emodin.

O. Issinger, Karsten Klopffleisch, Karsten Klopffleisch, 2008, Journal of molecular biology.

Crystal structure of the catalytic subunit of protein kinase CK2 from Zea mays at 2.1 Å resolution

D. Schomburg, O. Issinger, L. Pinna, 1998, The EMBO journal.

Synthesis, biological activity and structural study of new benzotriazole-based protein kinase CK2 inhibitors

R. Panchuk, M. Masłyk, A. Ramos, 2015 .

Insights from soft X-rays: the chlorine and sulfur sub-structures of a CK2α/DRB complex

O. Issinger, K. Niefind, J. Raaf, 2008, Molecular and Cellular Biochemistry.

A subnanomolar fluorescent probe for protein kinase CK2 interaction studies.

O. Issinger, K. Niefind, E. Enkvist, 2012, Organic & biomolecular chemistry.

Analyzing the interactome of human CK2β in prostate carcinoma cells reveals HSP70‐1 and Rho guanin nucleotide exchange factor 12 as novel interaction partners

C. Götz, J. Jose, K. Niefind, 2023, FASEB bioAdvances.

Structural Hypervariability of the Two Human Protein Kinase CK2 Catalytic Subunit Paralogs Revealed by Complex Structures with a Flavonol- and a Thieno[2,3-d]pyrimidine-Based Inhibitor †

S. Yarmoluk, A. Balanda, K. Niefind, 2017, Pharmaceuticals.

Impressions from the Conformational and Configurational Space Captured by Protein Kinase CK2

O. Issinger, K. Niefind, A. Schnitzler, 2015 .

The protein kinase CK2(Andante) holoenzyme structure supports proposed models of autoregulation and trans-autophosphorylation.

O. Issinger, B. Olsen, K. Niefind, 2014, Journal of molecular biology.

Enzymatic activity with an incomplete catalytic spine: insights from a comparative structural analysis of human CK2α and its paralogous isoform CK2α′

O. Issinger, B. Olsen, K. Niefind, 2011, Molecular and Cellular Biochemistry.

Conformational plasticity of the catalytic subunit of protein kinase CK2 and its consequences for regulation and drug design.

O. Issinger, K. Niefind, 2010, Biochimica et biophysica acta.

First inactive conformation of CK2 alpha, the catalytic subunit of protein kinase CK2.

O. Issinger, K. Niefind, J. Raaf, 2009, Journal of molecular biology.

Evolved to be active: sulfate ions define substrate recognition sites of CK2alpha and emphasise its exceptional role within the CMGC family of eukaryotic protein kinases.

O. Issinger, Olaf-Georg Issinger, K. Niefind, 2007, Journal of molecular biology.

Low-density crystal packing of human protein kinase CK2 catalytic subunit in complex with resorufin or other ligands: a tool to study the unique hinge-region plasticity of the enzyme without packing bias.

O. Issinger, Karsten Klopffleisch, K. Niefind, 2012, Acta crystallographica. Section D, Biological crystallography.

Structure of the human protein kinase CK2 catalytic subunit CK2α' and interaction thermodynamics with the regulatory subunit CK2β.

O. Issinger, B. Olsen, K. Niefind, 2011, Journal of molecular biology.

Inclining the purine base binding plane in protein kinase CK2 by exchanging the flanking side-chains generates a preference for ATP as a cosubstrate.

O. Issinger, Olaf-Georg Issinger, K. Niefind, 2005, Journal of molecular biology.

GTP plus water mimic ATP in the active site of protein kinase CK2

Dietmar Schomburg, Barbara Guerra, D. Schomburg, 1999, Nature Structural Biology.

Unexpected active-site flexibility in the structure of human neutrophil elastase in complex with a new dihydropyrimidone inhibitor.

Dietmar Schomburg, P. Reinemer, D. Schomburg, 2011, Journal of molecular biology.

The three-dimensional structure of AKR11B4, a glycerol dehydrogenase from Gluconobacter oxydans, reveals a tryptophan residue as an accelerator of reaction turnover.

W. Hummel, Nina Richter, K. Niefind, 2010, Journal of molecular biology.

The structure of an inverting GH43 beta-xylosidase from Geobacillus stearothermophilus with its substrate reveals the role of the three catalytic residues.

D. Schomburg, G. Shoham, Y. Shoham, 2006, Journal of molecular biology.

Crystal structure of L-2-hydroxyisocaproate dehydrogenase from Lactobacillus confusus at 2.2 A resolution. An example of strong asymmetry between subunits.

D. Schomburg, H. Hecht, K. Niefind, 1995, Journal of molecular biology.

Different roles of Enhanced Disease Susceptibility1 (EDS1) bound to and dissociated from Phytoalexin Deficient4 (PAD4) in Arabidopsis immunity.

J. Parker, B. Feys, N. Medina-Escobar, 2011, The New phytologist.

An EDS1 heterodimer signalling surface enforces timely reprogramming of immunity genes in Arabidopsis

Barbara Kracher, J. Parker, Patrick von Born, 2019, Nature Communications.

Structural and Enzymological Evidence for an Altered Substrate Specificity in Okur-Chung Neurodevelopmental Syndrome Mutant CK2αLys198Arg

J. Jose, K. Niefind, D. Lindenblatt, 2022, Frontiers in Molecular Biosciences.

Synthesis, biological properties and structural study of new halogenated azolo[4,5-b]pyridines as inhibitors of CK2 kinase.

K. Niefind, D. Lindenblatt, C. Toelzer, 2020, Bioorganic chemistry.

Mutation of recombinant catalytic subunit alpha of the protein kinase CK2 that affects catalytic efficiency and specificity.

D. Schomburg, N. Declerck, T. Chardot, 2000, Protein engineering.

A splicing variant of EDS1 from Vitis vinifera forms homodimers but no heterodimers with PAD4

L. Cseke, W. Gassmann, K. Niefind, 2023, Protein science : a publication of the Protein Society.

Crystal structure and snapshots along the reaction pathway of a family 51 alpha-L-arabinofuranosidase.

Dietmar Schomburg, D. Schomburg, G. Shoham, 2003, The EMBO journal.

Structural basis for the design of bisubstrate inhibitors of protein kinase CK2 provided by complex structures with the substrate-competitive inhibitor heparin.

K. Niefind, A. Schnitzler, 2021, European journal of medicinal chemistry.

PATTERNS OF SEQUENCE VARIATION IN FAMILIES OF HOMOLOGOUS PROTEINS

John P. Overington, Andrej Sali, Tom L. Blundell, 1991 .

Protein kinase CK2 inhibition is associated with the destabilization of HIF-1α in human cancer cells.

Y. Miyata, O. Issinger, N. Marcussen, 2015, Cancer letters.

Purification and characterization of the CK2alpha'-based holoenzyme, an isozyme of CK2alpha: a comparative analysis.

O. Issinger, B. Boldyreff, B. Olsen, 2006, Protein expression and purification.

Pentameric assembly of glycine receptor intracellular domains provides insights into gephyrin clustering

N. Havarushka, G. Schwarz, Alfredo Cabrera-Orefice, 2022, bioRxiv.

Crystal structure of cis‐biphenyl‐2,3‐dihydrodiol‐2,3‐dehydrogenase from a PCB degrader at 2.0 Å resolution

D. Schomburg, K. Timmis, H. Hecht, 1998, Protein science : a publication of the Protein Society.

High pH-value crystal structure of emodin in complex with the catalytic subunit of protein kinase CK2

O. Issinger, K. Niefind, J. Raaf, 2008 .

A novel esterase subfamily with α/β‐hydrolase fold suggested by structures of two bacterial enzymes homologous to l‐homoserine O‐acetyl transferases

J. Altenbuchner, H. Watzlawick, K. Niefind, 2016, FEBS letters.

Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor.

M. Gütschow, A. Abell, William Tieu, 2018, Acta crystallographica. Section F, Structural biology communications.

The structure of L-hydantoinase from Arthobacter aurescens leads to an understanding of dihydropyrimidinase substrate and enantio specificity.

D. Schomburg, C. Syldatk, M. Siemann, 2002, Biochemistry.

1.6 angstroms structure of an NAD+-dependent quinate dehydrogenase from Corynebacterium glutamicum.

D. Schomburg, K. Niefind, J. Schoepe, 2008, Acta crystallographica. Section D, Biological crystallography.

Cloning, expression, purification and preliminary crystallographic characterization of a shikimate dehydrogenase from Corynebacterium glutamicum.

D. Schomburg, K. Niefind, J. Schoepe, 2006, Acta crystallographica. Section F, Structural biology and crystallization communications.

Biochemical characterization of the recombinant human Drosophila homologues Timekeeper and Andante involved in the Drosophila circadian oscillator

O. Issinger, B. Boldyreff, K. Niefind, 2005, Molecular and Cellular Biochemistry.

Evidence for aggregation of protein kinase CK2 in the cell: a novel strategy for studying CK2 holoenzyme interaction by BRET2

O. Issinger, B. Guerra, M. Vrecl, 2014, Molecular and Cellular Biochemistry.

Crystallization and preliminary crystallographic analysis of cgHle, a homoserine acetyltransferase homologue, from Corynebacterium glutamicum.

J. Altenbuchner, H. Watzlawick, K. Niefind, 2009, Acta crystallographica. Section F, Structural biology and crystallization communications.

Improved protein-crystal identification by using 2,2,2-trichloroethanol as a fluorescence enhancer.

U. Baumann, K. Hofmann, K. Niefind, 2018, Acta crystallographica. Section F, Structural biology communications.

Unexpected Binding Mode of a Potent Indeno[1,2-b]indole-Type Inhibitor of Protein Kinase CK2 Revealed by Complex Structures with the Catalytic Subunit CK2α and Its Paralog CK2α′

J. Jose, K. Niefind, D. Lindenblatt, 2017, Pharmaceuticals.

Evidence for aggregation of protein kinase CK2 in the cell: a novel strategy for studying CK2 holoenzyme interaction by BRET2

O. Issinger, B. Guerra, M. Vrecl, 2014, Molecular and Cellular Biochemistry.

O. Issinger, Karsten Klopffleisch, K. Niefind, 2012, Acta crystallographica. Section D, Biological crystallography.

Interaction between CK2α and CK2β, the subunits of protein kinase CK2: thermodynamic contributions of key residues on the CK2α surface.

O. Issinger, D. Litchfield, Karsten Klopffleisch, 2011, Biochemistry.

Discovery and Exploration of Protein Kinase CK2 Binding Sites Using CK2α′Cys336Ser as an Exquisite Crystallographic Tool

K. Niefind, A. Uri, Kaido Viht, 2023, Kinases and Phosphatases.