I. Pikuleva

发表

Isolevuglandins and Mitochondrial Enzymes in the Retina

W. Liao, I. Turko, R. G. Salomon, 2011, The Journal of Biological Chemistry.

Brain Acetyl-CoA Production and Phosphorylation of Cytoskeletal Proteins Are Targets of CYP46A1 Activity Modulation and Altered Sterol Flux

I. Pikuleva, Natalia Mast, A. Petrov, 2021, Neurotherapeutics.

N,N-Dimethyl-3β-hydroxycholenamide Reduces Retinal Cholesterol via Partial Inhibition of Retinal Cholesterol Biosynthesis Rather Than its Liver X Receptor Transcriptional Activity

I. Pikuleva, Natalia Mast, Nicole El-Darzi, 2018, Front. Pharmacol..

Pathways of cholesterol homeostasis in mouse retina responsive to dietary and pharmacologic treatments[S]

I. Pikuleva, Wenchao Zheng, Natalia Mast, 2015, Journal of Lipid Research.

Steroid and protein ligand binding to cytochrome P450 46A1 as assessed by hydrogen-deuterium exchange and mass spectrometry.

N. Dodder, W. Liao, I. Turko, 2009, Biochemistry.

Characterizations of Hamster Retina as a Model for Studies of Retinal Cholesterol Homeostasis

Joseph Vance, I. Pikuleva, Natalia Mast, 2021, Biology.

Selective chemical modification of cytochrome P-450SCC lysine residues. Identification of lysines involved in the interaction with adrenodoxin.

S. Usanov, I. Pikuleva, V. Chashchin, 1989, Biochimica et biophysica acta.

Cytochrome P450s and cholesterol homeostasis.

I. Pikuleva, 2006, Pharmacology & therapeutics.

Changes in the levels of cerebral and extracerebral sterols in the brain of patients with Alzheimer's disease Published, JLR Papers in Press, October 1, 2003. DOI 10.1194/jlr.M300320-JLR200

T. Bayer, B. Winblad, N. Bogdanovic, 2004, Journal of Lipid Research.

Cholesterol binding to cytochrome P450 7A1, a key enzyme in bile acid biosynthesis.

J. Peterson, I. Pikuleva, S. Graham, 2005, Biochemistry.

Broad substrate specificity of human cytochrome P450 46A1 which initiates cholesterol degradation in the brain.

I. Pikuleva, Natalia Mast, M. Shou, 2003, Biochemistry.

A simple and rapid method to measure cholesterol binding to P450s and other proteins Published, JLR Papers in Press, April 16, 2005. DOI 10.1194/jlr.D500008-JLR200

I. Pikuleva, Natalia Mast, 2005, Journal of Lipid Research.

Cytochromes P450 in synthesis of steroid hormones, bile acids, vitamin D3 and cholesterol.

I. Pikuleva, M. Waterman, 1999, Molecular aspects of medicine.

Transcriptional and post-translational changes in the brain of mice deficient in cholesterol removal mediated by cytochrome P450 46A1 (CYP46A1)

I. Pikuleva, Natalia Mast, Kyle W. Anderson, 2017, PloS one.

Low-Dose Anti-HIV Drug Efavirenz Mitigates Retinal Vascular Lesions in a Mouse Model of Alzheimer’s Disease

I. Pikuleva, Natalia Mast, Nicole El-Darzi, 2022, Frontiers in Pharmacology.

Pretreatment with Pyridoxamine Mitigates Isolevuglandin-associated Retinal Effects in Mice Exposed to Bright Light*

H. Brunengraber, I. Turko, R. G. Salomon, 2013, The Journal of Biological Chemistry.

Quantification of cholesterol-metabolizing P450s CYP27A1 and CYP46A1 in neural tissues reveals a lack of enzyme-product correlations in human retina but not human brain.

Suber S. Huang, N. Dodder, W. Liao, 2011, Journal of proteome research.

Retinal Vascular Abnormalities and Microglia Activation in Mice with Deficiency in Cytochrome P450 46A1-Mediated Cholesterol Removal.

M. Grant, J. Busik, G. Trichonas, 2019, The American journal of pathology.

Conversion of 7-ketocholesterol to oxysterol metabolites by recombinant CYP27A1 and retinal pigment epithelial cells[S]

W. Liao, I. Turko, G. Heo, 2011, Journal of Lipid Research.

Cholesterol in mouse retina originates primarily from in situ de novo biosynthesis

H. Brunengraber, I. Björkhem, I. Pikuleva, 2016, Journal of Lipid Research.

Cholestenoic Acid is an important elimination product of cholesterol in the retina: comparison of retinal cholesterol metabolism with that in the brain.

Suber S. Huang, I. Pikuleva, Natalia Mast, 2011, Investigative ophthalmology & visual science.

Retinal Hypercholesterolemia Triggers Cholesterol Accumulation and Esterification in Photoreceptor Cells*

I. Pikuleva, Natalia Mast, A. Saadane, 2016, The Journal of Biological Chemistry.

In Silico and Intuitive Predictions of CYP46A1 Inhibition by Marketed Drugs with Subsequent Enzyme Crystallization in Complex with Fluvoxamine

C. Stout, J. Wiseman, I. Pikuleva, 2012, Molecular Pharmacology.

Retinal and nonocular abnormalities in Cyp27a1(-/-)Cyp46a1(-/-) mice with dysfunctional metabolism of cholesterol.

Suber S. Huang, T. Kern, N. Peachey, 2014, The American journal of pathology.

Structural Basis for Three-step Sequential Catalysis by the Cholesterol Side Chain Cleavage Enzyme CYP11A1*

K. Palczewski, C. Stout, A. Annalora, 2010, The Journal of Biological Chemistry.

Putative F–G loop is involved in association with the membrane in P450scc (P450 11A1)

I. Pikuleva, 2004, Molecular and Cellular Endocrinology.

An Additional Electrostatic Interaction between Adrenodoxin and P450c27 (CYP27A1) Results in Tighter Binding Than between Adrenodoxin and P450scc (CYP11A1)*

M. Waterman, I. Pikuleva, C. Cao, 1999, The Journal of Biological Chemistry.

Chemical modification of adrenocortical cytochrome P-450scc with tetranitromethane.

S. Usanov, I. Pikuleva, A. A. Akhrem, 1984, Biochimica et biophysica acta.

Increased Acetylcholine Levels and Other Brain Effects in 5XFAD Mice after Treatment with 8,14-Dihydroxy Metabolite of Efavirenz

I. Pikuleva, Natalia Mast, Yong Li, 2022, International journal of molecular sciences.

In Vitro Activation of Cytochrome P450 46A1 (CYP46A1) by Efavirenz-Related Compounds

F. Guengerich, I. Pikuleva, Natalia Mast, 2019, Journal of medicinal chemistry.

1 In Vitro Cytochrome P 450 46 A 1 ( CYP 46 A 1 ) Activation by Neuroactive Compounds

Kevin M. Johnson, F. Guengerich, I. Pikuleva, 2017 .

Pharmacologic Stimulation of Cytochrome P450 46A1 and Cerebral Cholesterol Turnover in Mice*

J. Wiseman, I. Pikuleva, Natalia Mast, 2013, The Journal of Biological Chemistry.

Stimulation of cerebral cholesterol turnover 1 Pharmacologic stimulation of cytochrome P 450 46 A 1 and cerebral cholesterol turnover in mice

J. Wiseman, I. Pikuleva, Natalia Mast, 2013 .

Marked variability in hepatic expression of cytochromes CYP7A1 and CYP27A1 as compared to cerebral CYP46A1. Lessons from a dietary study with omega 3 fatty acids in hamsters.

G. Ansari, W. Zaman, T. Wood, 2010, Biochimica et biophysica acta.

[Localization of tetranitromethane-modified tyrosine residues in the polypeptide chain of cholesterol-hydroxylating cytochrome P-450].

S. Usanov, I. Pikuleva, A. A. Akhrem, 1987, Bioorganicheskaia khimiia.

Studies of distant members of the P450 superfamily (P450scc and P450c27) by random chimeragenesis.

M. Waterman, I. Björkhem, I. Pikuleva, 1996, Archives of biochemistry and biophysics.

Inhibition and stimulation of activity of purified recombinant CYP11A1 by therapeutic agents

I. Pikuleva, Natalia Mast, M. Linger, 2013, Molecular and Cellular Endocrinology.

Cholesterol hydroperoxides as substrates for cholesterol-metabolizing cytochrome P450 enzymes and alternative sources of 25-hydroxycholesterol and other oxysterols.

J. V. van Lier, I. Pikuleva, Natalia Mast, 2015, Angewandte Chemie.

Active-site topology of bovine cholesterol side-chain cleavage cytochrome P450 (P450scc) and evidence for interaction of tyrosine 94 with the side chain of cholesterol.

P. Ortiz de Montellano, M. Waterman, N. Kagawa, 1995, Archives of biochemistry and biophysics.

7α-hydroxy-3-oxo-4-cholestenoic acid in cerebrospinal fluid reflects the integrity of the blood-brain barrier

P. Svenningsson, M. Bjerke, A. Wallin, 2014, Journal of Lipid Research.

Mechanism of Accumulation of Cholesterol and Cholestanol in Tendons and the Role of Sterol 27-hydroxylase (CYP27A1)

I. Björkhem, U. Diczfalusy, T. Movin, 2002, Arteriosclerosis, thrombosis, and vascular biology.

Synthesis and pharmacokinetic study of a 11C-labeled cholesterol 24-hydroxylase inhibitor using 'in-loop' [11C]CO2 fixation method.

Steven H. Liang, L. Josephson, Yihan Shao, 2020, Bioorganic & medicinal chemistry letters.

The antifungal drug voriconazole is an efficient inhibitor of brain cholesterol 24S-hydroxylase in vitro and in vivo

D. Lütjohann, I. Björkhem, O. Beck, 2010, Journal of Lipid Research.

Oxysterols in the circulation of patients with the Smith-Lemli-Opitz syndrome: abnormal levels of 24S- and 27-hydroxycholesterol.

D. Lütjohann, I. Björkhem, U. Diczfalusy, 2001, Journal of lipid research.

Novel sterols synthesized via the CYP27A1 metabolic pathway.

I. Pikuleva, N. Javitt, 2003, Archives of biochemistry and biophysics.

Assessment of cholesterol homeostasis in the living human brain

Steven H. Liang, Derek H. Oakley, A. Levey, 2022, Science Translational Medicine.

Distinct binding of cholesterol and 5β-cholestane-3α,7α,12α-triol to cytochrome P450 27A1 : Evidence from modeling and site-directed mutagenesis studies

J. Halpert, Hong Liu, J. Peterson, 2006 .

Putative helix F contributes to regioselectivity of hydroxylation in mitochondrial cytochrome P450 27A1.

I. Björkhem, I. Pikuleva, A. Puchkaev, 2001, Biochemistry.

Mass spectrometry detection of isolevuglandin adduction to specific protein residues.

C. Charvet, I. Pikuleva, 2015, Methods in molecular biology.

Metabolism of 4 beta -hydroxycholesterol in humans.

I. Björkhem, U. Diczfalusy, E. Ellis, 2002, The Journal of biological chemistry.

On the importance of albumin binding for the flux of 7α-hydroxy-3-oxo-4-cholestenoic acid in the brain

I. Björkhem, E. Edström, I. Pikuleva, 2017, Journal of Lipid Research.

Chemical modification of rabbit IgG with N-dansylaziridine. Investigation of the properties of dansylated antibodies.

I. Turko, I. Pikuleva, V. Chashchin, 1991, Molecular immunology.

CYP46A1 activation by low-dose efavirenz enhances brain cholesterol metabolism in subjects with early Alzheimer’s disease

C. Tatsuoka, A. Lerner, A. Pieper, 2022, Alzheimer's Research & Therapy.

Escherichia coli flavodoxin sepharose as an affinity resin for cytochromes P450 and use to identify a putative cytochrome P450c17/3beta-hydroxysteroid dehydrogenase interaction.

M. Waterman, N. Kagawa, C. M. Jenkins, 1997, Archives of biochemistry and biophysics.

Human cytochromes P450

P. Beaune, M. Waterman, R. Estabrook, 1999 .

Antifungal Azoles: Structural Insights into Undesired Tight Binding to Cholesterol-Metabolizing CYP46A1

C. Stout, I. Pikuleva, Wenchao Zheng, 2013, Molecular Pharmacology.

Binding of a Cyano- and Fluoro-containing Drug Bicalutamide to Cytochrome P450 46A1

C. Stout, I. Pikuleva, Wenchao Zheng, 2013, The Journal of Biological Chemistry.

Bicalutamide-CYP 46 A 1 interactions 1 Binding of a Cyano-and Fluoro-Containing Drug Bicalutamide to Cytochrome P 450 46 A 1 : Unusual Features and Spectral Response *

C. Stout, I. Pikuleva, Wenchao Zheng, 2013 .

Crystal structures of substrate-bound and substrate-free cytochrome P450 46A1, the principal cholesterol hydroxylase in the brain

Eric F. Johnson, C. Stout, I. Pikuleva, 2008, Proceedings of the National Academy of Sciences.

Cholesterol Hydroxylating Cytochrome P450 46A1: From Mechanisms of Action to Clinical Applications

N. Cartier, I. Pikuleva, 2021, Frontiers in Aging Neuroscience.

Genetically engineered bacterial cells and applications.

M. Waterman, C. M. Jenkins, I. Pikuleva, 1995, Toxicology letters.

CYP46A1 Activation by Efavirenz Leads to Behavioral Improvement without Significant Changes in Amyloid Plaque Load in the Brain of 5XFAD Mice

I. Pikuleva, Natalia Mast, A. Petrov, 2019, Neurotherapeutics.

Functional reconstitution of cytochrome P-450scc with hemin activated with Woodward's reagent K. Formation of a hemeprotein cross-link.

I. Pikuleva, V. Chashchin, A. Lapko, 1992, The Journal of biological chemistry.

Effects of a Disrupted Blood-Brain Barrier on Cholesterol Homeostasis in the Brain*

C. Betsholtz, W. Griffiths, Yuqin Wang, 2014, The Journal of Biological Chemistry.

The tertiary structure of full-length bovine adrenodoxin suggests functional dimers.

M. Waterman, Y. Kim, M R Waterman, 2000, Archives of biochemistry and biophysics.

CYP46A1-dependent and independent effects of efavirenz treatment

I. Pikuleva, Natalia Mast, A. Petrov, 2020, Brain communications.

Marketed Drugs Can Inhibit Cytochrome P450 27A1, a Potential New Target for Breast Cancer Adjuvant Therapy

I. Pikuleva, Natalia Mast, Joseph B Lin, 2015, Molecular Pharmacology.

Spatial Distribution of the Pathways of Cholesterol Homeostasis in Human Retina

Suber S. Huang, C. Curcio, C. Charvet, 2012, PloS one.

In vitro cytochrome P450 46A1 (CYP46A1) activation by neuroactive compounds

Kevin M. Johnson, F. Guengerich, I. Pikuleva, 2017, The Journal of Biological Chemistry.

Cholesterol 24-Hydroxylation by CYP46A1: Benefits of Modulation for Brain Diseases

I. Pikuleva, A. Petrov, 2019, Neurotherapeutics.

Cholesterol-metabolizing cytochromes P450: implications for cholesterol lowering

I. Pikuleva, 2008, Expert opinion on drug metabolism & toxicology.

Mechanisms that minimize retinal impact of apolipoprotein E absence[S]

J. Dunaief, I. Pikuleva, Natalia Mast, 2018, Journal of Lipid Research.

The Interplay between Retinal Pathways of Cholesterol Output and Its Effects on Mouse Retina

I. Pikuleva, Natalia Mast, A. Petrov, 2019, Biomolecules.

Metabolism of 4β-Hydroxycholesterol in Humans*

I. Björkhem, U. Diczfalusy, E. Ellis, 2002, The Journal of Biological Chemistry.

The key genes, phosphoproteins, processes, and pathways affected by efavirenz‐activated CYP46A1 in the amyloid‐decreasing paradigm of efavirenz treatment

I. Pikuleva, Natalia Mast, A. Petrov, 2019, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

Studies of ApoD−/− and ApoD−/−ApoE−/− mice uncover the APOD significance for retinal metabolism, function, and status of chorioretinal blood vessels

I. Pikuleva, Natalia Mast, A. Petrov, 2020, Cellular and Molecular Life Sciences.

Misdiagnosis of CTX due to propofol: The interference of total intravenous propofol anaesthesia with bile acid profiling

J. Jans, I. Pikuleva, M. van der Ham, 2020, Journal of inherited metabolic disease.

Structural and Biochemical Characterization of Mycobacterium tuberculosis CYP142

A. Munro, D. Leys, C. Levy, 2010, The Journal of Biological Chemistry.

The Structure of Mycobacterium tuberculosis CYP125

A. Munro, D. Leys, C. Levy, 2009, The Journal of Biological Chemistry.

Structural and biochemical characterization of Mycobacterium tuberculosis CYP142: Evidence for multiple cholesterol 27-hydroxylase activities in a human pathogen

A. Munro, D. Leys, C. Levy, 2010 .

CYP46A1 Activation by Efavirenz Leads to Behavioral Improvement without Significant Changes in Amyloid Plaque Load in the Brain of 5XFAD Mice

I. Pikuleva, Natalia Mast, A. Petrov, 2019, Neurotherapeutics.

Marked accumulation of 27-hydroxycholesterol in the brains of Alzheimer's patients with the Swedish APP 670/671 mutation

B. Winblad, A. Nordberg, I. Björkhem, 2011, Journal of Lipid Research.

CHOLESTEROL-METABOLIZING CYTOCHROMES P450

I. Pikuleva, 2006, Drug Metabolism and Disposition.

Expression of human cytochrome P450 46A1 in Escherichia coli: effects of N- and C-terminal modifications.

I. Pikuleva, Natalia Mast, K. Nakayama, 2004, Archives of biochemistry and biophysics.

Cholesterol-metabolizing enzyme cytochrome P450 46A1 as a pharmacologic target for Alzheimer's disease

G. Landreth, I. Pikuleva, Natalia Mast, 2017, Neuropharmacology.

Cholesterol in the retina: The best is yet to come

C. Curcio, I. Pikuleva, 2014, Progress in Retinal and Eye Research.

Cytochromes P450: Roles in Diseases*

M. Waterman, I. Pikuleva, 2013, The Journal of Biological Chemistry.

Localization of sterols and oxysterols in mouse brain reveals distinct spatial cholesterol metabolism

D. Skibinski, M. Clench, W. Griffiths, 2020, Proceedings of the National Academy of Sciences.

Imaging Sterols and Oxysterols in Mouse Brain Reveals Distinct Spatial Cholesterol Metabolism

D. Skibinski, M. Clench, W. Griffiths, 2018 .

Imaging Oxysterols in Mouse Brain by On-Tissue Derivatisation – Robotic Liquid Micro-Extraction Surface Analysis – Liquid Chromatography Mass Spectrometry

M. Clench, W. Griffiths, Yuqin Wang, 2018, bioRxiv.

Histone H3 Ser57 and Thr58 phosphorylation in the brain of 5XFAD mice

I. Turko, I. Pikuleva, Natalia Mast, 2015, FEBS open bio.

2-Hydroxypropyl-β-cyclodextrin mitigates pathological changes in a mouse model of retinal cholesterol dyshomeostasis

J. Busik, I. Pikuleva, Natalia Mast, 2022, Journal of Lipid Research.

2-Hydroxypropyl-β-cyclodextrin mitigates pathological changes in a mouse model of retinal cholesterol dyshomeostasis

J. Busik, I. Pikuleva, Natalia Mast, 2022, Journal of lipid research.

2‐Hydroxypropyl‐β‐cyclodextrin reduces retinal cholesterol in wild‐type and Cyp27a1−/−Cyp46a1−/− mice with deficiency in the oxysterol production

I. Pikuleva, Natalia Mast, A. Petrov, 2020, British journal of pharmacology.

Mass spectrometry quantification of PICALM and AP180 in human frontal cortex and neural retina.

I. Turko, I. Pikuleva, Junjun Chen, 2013, Analytical biochemistry.

Quantification of Histone Deacetylase Isoforms in Human Frontal Cortex, Human Retina, and Mouse Brain

I. Turko, I. Pikuleva, Natalia Mast, 2015, PloS one.

Retinal Cholesterol Content Is Reduced in Simvastatin-Treated Mice Due to Inhibited Local Biosynthesis Albeit Increased Uptake of Serum Cholesterol

I. Pikuleva, Natalia Mast, I. Bederman, 2018, Drug Metabolism and Disposition.

Abnormal vascularization in mouse retina with dysregulated retinal cholesterol homeostasis.

Suber S. Huang, N. Peachey, C. Charvet, 2012, The Journal of clinical investigation.

Targeting cytochrome P450 46A1 and brain cholesterol 24-hydroxylation to treat neurodegenerative diseases.

I. Pikuleva, 2021, Exploration of neuroprotective therapy.

Activities of Recombinant Human Cytochrome P450c27 (CYP27) Which Produce Intermediates of Alternative Bile Acid Biosynthetic Pathways*

M. Waterman, I. Björkhem, I. Pikuleva, 1998, The Journal of Biological Chemistry.

Novel route for elimination of brain oxysterols across the blood-brain barrier: conversion into 7α-hydroxy-3-oxo-4-cholestenoic acid Published, JLR Papers in Press, January 24, 2007.

Wolfgang Sattler, Lena Ekström, Steve Meaney, 2007, Journal of Lipid Research.

Brain sterol flux mediated by cytochrome P450 46A1 affects membrane properties and membrane-dependent processes

I. Pikuleva, Natalia Mast, A. Petrov, 2020, Brain communications.

Membrane-Protein Interactions Contribute to Efficient 27-Hydroxylation of Cholesterol by Mitochondrial Cytochrome P450 27A1*

Werner Braun, Catherine H Schein, C. Schein, 2002, The Journal of Biological Chemistry.

Structural Basis of Drug Binding to CYP46A1, an Enzyme That Controls Cholesterol Turnover in the Brain*

C. Stout, C. Charvet, I. Pikuleva, 2010, The Journal of Biological Chemistry.

Features of the retinal environment which affect the activities and product profile of cholesterol-metabolizing cytochromes P450 CYP27A1 and CYP11A1.

W. Liao, I. Turko, G. Heo, 2012, Archives of biochemistry and biophysics.

Use of complementary cation and anion heavy-atom salt derivatives to solve the structure of cytochrome P450 46A1

Eric F. Johnson, C. Stout, I. Pikuleva, 2008, Acta Crystallographica Section D: Biological Crystallography.

Phospholipids modify substrate binding and enzyme activity of human cytochrome P450 27A1 Published, JLR Papers in Press, September 1, 2004. DOI 10.1194/jlr.M400300-JLR200

G. Ansari, I. Pikuleva, U. Andersson, 2004, Journal of Lipid Research.

Membrane Binding and Substrate Access Merge in Cytochrome P450 7A1, a Key Enzyme in Degradation of Cholesterol*

I. Pikuleva, K. Nakayama, Andrei Puchkaev, 2001, The Journal of Biological Chemistry.

Cytochrome P450 27A1 Deficiency and Regional Differences in Brain Sterol Metabolism Cause Preferential Cholestanol Accumulation in the Cerebellum*

C. Tatsuoka, I. Turko, I. Pikuleva, 2017, The Journal of Biological Chemistry.

Cholesterol 24-Hydroxylation by CYP46A1: Benefits of Modulation for Brain Diseases

I. Pikuleva, A. Petrov, 2019, Neurotherapeutics.

Metabolism of a novel side chain modified Delta8(14)-15-ketosterol, a potential cholesterol lowering drug: 28-hydroxylation by CYP27A1.

I. Björkhem, I. Pikuleva, A. Misharin, 2008, Biochimica et biophysica acta.

Expression, purification, and enzymatic properties of recombinant human cytochrome P450c27 (CYP27).

M. Waterman, I. Björkhem, I. Pikuleva, 1997, Archives of biochemistry and biophysics.

Quantitative characterizations of the cholesterol-related pathways in the retina and brain of hamsters

I. Pikuleva, Natalia Mast, Nicole El-Darzi, 2023, Journal of lipid research.

The normalizing effects of the CYP46A1 activator efavirenz on retinal sterol levels and risk factors for glaucoma in Apoj−/− mice

D. Rhee, I. Pikuleva, Natalia Mast, 2023, Cellular and Molecular Life Sciences.

Cholesterol crystal formation is a unifying pathogenic mechanism in the development of diabetic retinopathy

D. Agrawal, M. Grant, Yvonne Adu-Agyeiwaah, 2023, Diabetologia.

Drugs and Scaffold That Inhibit Cytochrome P450 27A1 In Vitro and In Vivo

I. Pikuleva, Natalia Mast, Morrie Lam, 2018, Molecular Pharmacology.

Drugs and Scaffold that Inhibit Cytochrome P450 27A1 (CYP27A1) in Vitro and in Vivo

I. Pikuleva, Natalia Mast, Morrie Lam, 2017 .

Expression of eukaryotic cytochromes P450 in E. coli.

M. Waterman, C. M. Jenkins, I. Pikuleva, 1998, Methods in molecular biology.

Sample prefractionation for mass spectrometry quantification of low-abundance membrane proteins.

I. Turko, G. Heo, I. Pikuleva, 2012, Analytical chemistry.

Optimizing the conditions of a multiple reaction monitoring assay for membrane proteins: quantification of cytochrome P450 11A1 and adrenodoxin reductase in bovine adrenal cortex and retina.

N. Dodder, W. Liao, I. Turko, 2010, Analytical chemistry.

Distinct binding of cholesterol and 5beta-cholestane-3alpha,7alpha,12alpha-triol to cytochrome P450 27A1: evidence from modeling and site-directed mutagenesis studies.

J. Halpert, Hong Liu, J. Peterson, 2006, Biochemistry.

Imaging Sterols and Oxysterols in Mouse Brain Reveals Distinct Spatial Cholesterol Metabolism

D. Skibinski, M. Clench, Mark Baumert, 2019 .

Imaging Oxysterols in Mouse Brain by On-Tissue Derivatisation – Robotic Micro-Liquid-Extraction Surface Analysis – Liquid Chromatography Mass Spectrometry

M. Clench, Mark Baumert, O. Howell, 2019 .

Cholesterol in the retina: The best is yet to come

C. Curcio, I. Pikuleva, 2014, Progress in Retinal and Eye Research.

Features of the retinal environment which affect the activities and product profile of cholesterol-metabolizing cytochromes P450 CYP27A1 and CYP11A1.

W. Liao, I. Turko, G. Heo, 2012, Archives of biochemistry and biophysics.

Mapping of the Allosteric Site in Cholesterol Hydroxylase CYP46A1 for Efavirenz, a Drug That Stimulates Enzyme Activity*

J. W. Hudgens, I. Turko, I. Pikuleva, 2016, The Journal of Biological Chemistry.

Combined use of mass spectrometry and heterologous expression for identification of membrane-interacting peptides in cytochrome P450 46A1 and NADPH-cytochrome P450 oxidoreductase.

W. Liao, I. Turko, I. Pikuleva, 2009, Archives of biochemistry and biophysics.

Studies of Membrane Topology of Mitochondrial Cholesterol Hydroxylases CYPs 27A1 and 11A1

W. Liao, I. Turko, I. Pikuleva, 2008, Lipids.

Posttranslational modification by an isolevuglandin diminishes activity of the mitochondrial cytochrome P450 27A1[S]

R. G. Salomon, C. Charvet, James M. Laird, 2013, Journal of Lipid Research.

Isolevuglandins and Mitochondrial Enzymes in the Retina

W. Liao, I. Turko, R. G. Salomon, 2011, The Journal of Biological Chemistry.

Unbiased Insights into the Multiplicity of the CYP46A1 Brain Effects in 5XFAD Mice Treated with Low Dose Efavirenz

I. Pikuleva, Natalia Mast, Makaya Butts, 2024, Journal of Lipid Research.

Challenges and Opportunities in P450 Research on the Eye

I. Pikuleva, 2023, Drug Metabolism and Disposition.

APOB100 transgenic mice exemplify how the systemic circulation content may affect the retina without altering retinal cholesterol input

I. Pikuleva, Natalia Mast, Nicole El-Darzi, 2024, Cellular and molecular life sciences : CMLS.