J. Guisán

Manuel Fuentes, Roberto Fernandez-Lafuente, R. Fernández-Lafuente, 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Immobilization–stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus

F. Cava, R. Fernández-Lafuente, J. Rocha-Martín, 2008, Applied Microbiology and Biotechnology.

Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method.

R. Fernández-Lafuente, C. Rosell, J. Guisan, 1993, Enzyme and microbial technology.

Development of a high efficient biocatalyst by oriented covalent immobilization of a novel recombinant 2'-N-deoxyribosyltransferase from Lactobacillus animalis.

F. López‐Gallego, J. Guisán, J. Trelles, 2018, Journal of biotechnology.

Immobilization of Proteins on Highly Activated Glyoxyl Supports: Dramatic Increase of the Enzyme Stability via Multipoint Immobilization on Pre-existing Carriers

F. López‐Gallego, J. Rocha-Martín, G. Fernandez-Lorente, 2015 .

Stabilization of enzymes by multipoint covalent immobilization on supports activated with glyoxyl groups.

F. López‐Gallego, J. Rocha-Martín, G. Fernandez-Lorente, 2013, Methods in molecular biology.

Improvement of fungal arabinofuranosidase thermal stability by reversible immobilization

M. Polizeli, A. Damasio, F. Segato, 2012 .

Heterogeneous Enzyme Kinetics

R. Fernández-Lafuente, A. Illanes, L. Wilson, 2008 .

Improved purification and enzymatic properties of a mixture of Sticholysin I and II: isotoxins with hemolytic and phospholipase A(2) activities from the sea anemone Stichodactyla helianthus.

M. E. Lanio, C. Álvarez, J. Guisán, 2014, Protein expression and purification.

Production of Hesperetin Using a Covalently Multipoint Immobilized Diglycosidase from Acremonium sp. DSM24697

J. Breccia, F. López‐Gallego, Lucrecia Piñuel, 2013, Journal of Molecular Microbiology and Biotechnology.

Derepression and repression of the histidine operon: role of the feedback site of the first enzyme

A. Ballesteros, J. Guisán, A. R. Tébar, 1975, Journal of bacteriology.

Production of new nanobiocatalysts via immobilization of lipase B from C. antarctica on polyurethane nanosupports for application on food and pharmaceutical industries.

D. de Oliveira, E. A. Manoel, A. Valério, 2020, International journal of biological macromolecules.

Synthesis and modification of polyurethane for immobilization of Thermomyces lanuginosus (TLL) lipase for ethanolysis of fish oil in solvent free system

D. Oliveira, A. Valério, C. Sayer, 2015 .

Intense PEGylation of Enzyme Surfaces: Relevant Stabilizing Effects.

G. Fernandez-Lorente, M. Romero-Fernández, J. Guisán, 2016, Methods in enzymology.

Selective Ethanolysis of Fish Oil Catalyzed by Immobilized Lipases

G. Fernandez-Lorente, J. Guisán, S. Moreno-Perez, 2014 .

Immobilization and biochemical properties of a β-xylosidase activated by glucose/xylose from Aspergillus niger USP-67 with transxylosylation activity

J. A. Jorge, M. Polizeli, C. Mateo, 2013 .

Influence of different immobilization techniques to improve the enantioselectivity of lipase from Geotrichum candidum applied on the resolution of mandelic acid

G. Fernandez-Lorente, B. Pessela, W. G. de Morais Júnior, 2018, Molecular Catalysis.

Two step ethanolysis: A simple and efficient way to improve the enzymatic biodiesel synthesis catalyzed by an immobilized–stabilized lipase from Thermomyces lanuginosus

Roberto Fernandez-Lafuente, R. Rodrigues, R. Fernández-Lafuente, 2010 .

Thermus thermophilus as a Cell Factory for the Production of a Thermophilic Mn-Dependent Catalase Which Fails To Be Synthesized in an Active Form in Escherichia coli

F. Cava, R. Fernández-Lafuente, L. Betancor, 2004, Applied and Environmental Microbiology.

Stabilization of the quaternary structure of a hexameric alpha-galactosidase from Thermus sp. T2 by immobilization and post-immobilization techniques

R. Fernández-Lafuente, A. Carrascosa, C. Mateo, 2008 .

Production of a Thermoresistant Alpha-galactosidase from Thermus sp. Strain T2 for Food Processing

J. García, R. Torres, R. Fernández-Lafuente, 2007 .

Sugarcane Bagasse Saccharification by Enzymatic Hydrolysis Using Endocellulase and β-glucosidase Immobilized on Different Supports

N. Caetano, Wilson G. Morais Júnior, T. F. Pacheco, 2021, Catalysts.

Cloning, expression and immobilization of glutamate racemase from Lactobacillus fermenti for the production of D-glutamate from L-glutamate

J. García, M. Mansur, E. García-Calvo, 2004, Biotechnology Letters.

Genetic Modification of the Penicillin G Acylase Surface To Improve Its Reversible Immobilization on Ionic Exchangers

J. García, B. Galán, R. Fernández-Lafuente, 2006, Applied and Environmental Microbiology.

Multi-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Stabilization of Industrial Enzymes.

F. López‐Gallego, J. Rocha-Martín, G. Fernandez-Lorente, 2020, Methods in molecular biology.

Reversible immobilization of a hexameric α-galactosidase from Thermus sp. strain T2 on polymeric ionic exchangers

R. Fernández-Lafuente, A. Carrascosa, C. Mateo, 2008 .

Overproduction of Thermus sp. Strain T2 β-Galactosidase in Escherichia coli and Preparation by Using Tailor-Made Metal Chelate Supports

J. García, R. Fernández-Lafuente, A. Carrascosa, 2003, Applied and Environmental Microbiology.

Improving Properties of a Novel β-Galactosidase from Lactobacillus plantarum by Covalent Immobilization

J. Curiel, B. de Las Rivas, R. Muñoz, 2015, Molecules.

Critical Role of Different Immobilized Biocatalysts of a Given Lipase in the Selective Ethanolysis of Sardine Oil.

Sonia Moreno-Perez, F. J. Señoráns, P. Luna, 2017, Journal of agricultural and food chemistry.

The Science of Enzyme Immobilization.

F. López‐Gallego, J. Rocha-Martín, G. Fernandez-Lorente, 2020, Methods in molecular biology.

The coimmobilization of d-amino acid oxidase and catalase enables the quantitative transformation of d-amino acids (d-phenylalanine) into α-keto acids (phenylpyruvic acid)

Roberto Fernandez-Lafuente, R. Fernández-Lafuente, V. Rodrı́guez, 1998 .

Preparation of immobilized sepharose-micrococcal nuclease derivatives: activity and stability

A. Ballesteros, J. Guisán, 1979 .

Reversible and Strong Immobilization of Proteins by Ionic Exchange on Supports Coated with Sulfate‐Dextran

Olga Abian, Manuel Fuentes, Rodrigo Torres, 2004, Biotechnology progress.

Structural and Functional Stabilization of L‐Asparaginase via Multisubunit Immobilization onto Highly Activated Supports

R. Fernández-Lafuente, V. Balcão, F. Malcata, 2001, Biotechnology progress.

Optimization of theoretical maximal quantity of cells to immobilize on solid supports in the rational design of immobilized derivatives strategy

J. Guisán, Alberto del Monte-Martínez, Irina Salgado-Bernal, 2021, World Journal of Microbiology and Biotechnology.

Stabilizing effect of penicillin G sulfoxide, a competitive inhibitor of penicillin G acylase: its practical applications.

R. Fernández-Lafuente, G. Álvaro, R. M. Blanco, 1991, Enzyme and microbial technology.

Tips for the functionalization of nanoparticles with antibodies.

P. Batalla, J. M. de la Fuente, V. Grazú, 2013, Methods in molecular biology.

Peptide Synthesis in Organic-Aqueous Media Catalysed by α-Chymotrypsin Immobilised Over Different Supports

A. Alcántara, M. H. Gil, J. Guisán, 1992 .

High stabilization of immobilized Rhizomucor miehei lipase by additional coating with hydrophilic crosslinked polymers: Poly-allylamine/Aldehyde–dextran

J. Rocha-Martín, G. Fernandez-Lorente, J. Guisán, 2020 .

Modulation of the catalytic properties of multimeric β-galactosidase from E. coli by using different immobilization protocols

Manuel Fuentes, Roberto Fernandez-Lafuente, R. Fernández-Lafuente, 2007 .

New opportunities for immobilization of enzymes.

J. Guisán, 2013, Methods in molecular biology.

Oriented immobilization of antibodies onto sensing platforms - A critical review.

J. Rocha-Martín, J. Guisán, Shipeng Gao, 2021, Analytica chimica acta.

Partial Purification of Crude Lipase Extract from Yarrowia lipolytica: Precipitation, Aqueous Two-Phase Systems (ATPS), and Immobilization Methods

B. Ribeiro, V. Melo, J. Guisán, 2023, Cleaner Chemical Engineering.

Interaction of the antitumor drug 9-aminoacridine with guanidinobenzoatase studied by spectroscopic methods: a possible tumor marker probe based on the fluorescence exciplex emission.

G. Rivas, S. Sánchez‐Cortés, J. García-Ramos, 2000, Biochemistry.

Immobilization and Stabilization of a Cyclodextrin Glycosyltransferase by Covalent Attachment on Highly Activated Glyoxyl‐Agarose Supports

R. Fernández-Lafuente, L. Wilson, C. Mateo, 2006, Biotechnology progress.

Immobilization–stabilization of glucoamylase: Chemical modification of the enzyme surface followed by covalent attachment on highly activated glyoxyl-agarose supports

G. Zanin, L. Betancor, M. F. Vieira, 2011 .

Stabilization of Glycosylated β-Glucosidase by Intramolecular Crosslinking Between Oxidized Glycosidic Chains and Lysine Residues

C. Farinas, P. Tardioli, G. Fernandez-Lorente, 2020, Applied Biochemistry and Biotechnology.

Immobilization of Enzymes on Supports Activated with Glutaraldehyde: A Very Simple Immobilization Protocol.

L. Betancor, F. López‐Gallego, J. Guisán, 2020, Methods in molecular biology.

Immobilization and stabilization of commercial β-1,4-endoxylanase Depol™ 333MDP by multipoint covalent attachment for xylan hydrolysis: Production of prebiotics (xylo-oligosaccharides)

J. Rocha-Martín, G. Fernandez-Lorente, M. Romero-Fernández, 2018 .

Synthesis of ascorbyl oleate by transesterification of olive oil with ascorbic acid in polar organic media catalyzed by immobilized lipases.

G. Fernandez-Lorente, J. Guisán, M. Filice, 2013, Chemistry and physics of lipids.

A Novel Halophilic Lipase, LipBL, Showing High Efficiency in the Production of Eicosapentaenoic Acid (EPA)

A. Ventosa, M. T. García, G. Fernandez-Lorente, 2011, PloS one.

Isolation and Screening of Filamentous Fungi Producing Extracellular Lipase with Potential in Biodiesel Production

B. Pessela, A. F. Júnior, J. Guisán, 2015 .

β-Glucosidase immobilized and stabilized on agarose matrix functionalized with distinct reactive groups

G. Zanin, M. F. Vieira, P. Tardioli, 2011 .

Covalent immobilization of antibodies on finally inert support surfaces through their surface regions having the highest densities in carboxyl groups.

R. Fernández-Lafuente, P. Batalla, C. Mateo, 2008, Biomacromolecules.

Optimization of theoretical maximal quantity of cells to immobilize on solid supports in the rational design of immobilized derivatives strategy

J. Guisán, Alberto del Monte-Martínez, Irina Salgado-Bernal, 2021, World journal of microbiology & biotechnology.

Degradation and denaturation of stable enzymes

R. Fernández-Lafuente, D. Cowan, R. Daniel, 1998 .

PRODUCTION OF XYLO-OLIGOSACCHARIDES 1 BY IMMOBILIZED-STABILIZED DERIVATIVES OF 2 ENDO-XYLANASE FROM Streptomyces halstedii 3 4

R. Monti, A. I. Ruiz-Matute, M. Díaz, 2012 .

Enzyme reaction engineering: design of peptide synthesis by stabilized trypsin.

G. Álvaro, R. M. Blanco, J. Guisán, 1991, Enzyme and microbial technology.

Immobilization-stabilization of proteases as a tool to improve the industrial design of peptide synthesis.

R. Fernández-Lafuente, C. Rosell, G. Álvaro, 1991, Biomedica biochimica acta.

Improving the Industrial Production of 6‐APA: Enzymatic Hydrolysis of Penicillin G in the Presence of Organic Solvents

O. Abián, R. Fernández-Lafuente, G. Fernandez-Lorente, 2003, Biotechnology progress.

IMMOBILIZATION-STABILIZATION OF PENICILLIN G ACYLASE FROM E. COLI

G. Álvaro, R. M. Blanco, J. Guisán, 1990 .

Lipase-Catalyzed Regioselective One-Step Synthesis of Penta-O-acetyl-3-hydroxylactal

R. Vanna, J. Palomo, J. Guisán, 2009 .

Simple Purification of Immunoglobulins from Whey Proteins Concentrate

R. Torres, R. Fernández-Lafuente, P. Batalla, 2006, Biotechnology progress.

Evaluation of Different Glutaryl Acylase Mutants to Improve the Hydolysis of Cephalosporin C in the Absence of Hydrogen Peroxide

Wim J. Quax, Carlos R. Reis, Fernando López-Gallego, 2008 .

Engineering the D-amino acid oxidase from Trigonopsis variabilis to facilitate its overproduction in Escherichia coli and its downstream processing by tailor-made metal chelate supports

J. García, J. Barredo, B. Díez, 1999 .

Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices.

B. Nidetzky, A. Hidalgo, Juan M. Bolívar, 2018, ACS applied materials & interfaces.

Enzyme Surface Glycosylation in the Solid Phase: Improved Activity and Selectivity of Candida Antarctica Lipase B

O. Abián, M. Gutarra, D. Freire, 2011 .

Hydrolysis of nucleic acids by sepharose-micrococcal endonuclease

A. Ballesteros, J. Guisán, 1981 .

Engineering the Substrate Specificity of a Thermophilic Penicillin Acylase from Thermus thermophilus

Leticia L. Torres, F. López‐Gallego, A. Marina, 2012, Applied and Environmental Microbiology.

Thermus thermophilus Thermophilic Penicillin Acylase from Engineering the Substrate Specificity of a

Leticia L. Torres, F. López‐Gallego, A. Marina, 2013 .

Ethyl esters production catalyzed by immobilized lipases is influenced by n-hexane and ter-amyl alcohol as organic solvents

D. A. Bocchini, E. Gomes, G. Fernandez-Lorente, 2020, Bioprocess and Biosystems Engineering.

Heterofunctional supports for the one-step purification, immobilization and stabilization of large multimeric enzymes: Amino-glyoxyl versus amino-epoxy supports

A. Carrascosa, C. Mateo, B. Pessela, 2010 .

Reversible Immobilization of Glutaryl Acylase on Sepabeads Coated with Polyethyleneimine

R. Fernández-Lafuente, N. Alonso-Morales, L. Betancor, 2008, Biotechnology progress.

Co-Immobilization and Co-Localization of Multi-Enzyme Systems on Porous Materials.

F. López‐Gallego, J. Rocha-Martín, G. Fernandez-Lorente, 2020, Methods in molecular biology.

New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD+-recycling enzyme

F. López‐Gallego, J. Rocha-Martín, A. Hidalgo, 2011, BMC biotechnology.

Immobilization of the acylase from Escherichia coli on glyoxyl-agarose gives efficient catalyst for the synthesis of cephalosporins.

R. Fernández-Lafuente, A. Alcántara, L. Toma, 2008, Enzyme and microbial technology.

Hydrolysis and oxidation of racemic esters into prochiral ketones catalyzed by a consortium of immobilized enzymes

E. Favela-Torres, F. López‐Gallego, J. Rocha-Martín, 2016 .

Co-localization of oxidase and catalase inside a porous support to improve the elimination of hydrogen peroxide: Oxidation of biogenic amines by amino oxidase from Pisum sativum.

J. Rocha-Martín, G. Fernandez-Lorente, J. Guisán, 2018, Enzyme and microbial technology.

Enzymatic synthesis of amoxicillin: avoiding limitations of the mechanistic approach for reaction kinetics.

Ruy Sousa, Roberto Fernandez-Lafuente, R. Fernández-Lafuente, 2002, Biotechnology and bioengineering.

Determination of protein‐protein interactions through aldehyde‐dextran intermolecular cross‐linking

Olga Abian, Manuel Fuentes, Aurelio Hidalgo, 2004, Proteomics.

Purification, Immobilization, Stabilization and Characterization of Commercial Extract with Î²-galactosidase Activity

A. Carrascosa, C. Mateo, B. Pessela, 2013 .

Agarose-chymotrypsin as a catalyst for peptide and amino acid ester synthesis in organic media

P. Halling, J. Guisan, R. M. Blanco, 1989, Biotechnology Letters.

Microbial Enzyme: Applications in Industry and in Bioremediation

Claudiu T. Supuran, C. Supuran, Alane Beatriz Vermelho, 2012, Enzyme research.

Glutaraldehyde-mediated protein immobilization.

L. Betancor, F. López‐Gallego, J. Guisán, 2013, Methods in molecular biology.

Oriented Attachment of Recombinant Proteins to Agarose-Coated Magnetic Nanoparticles by Means of a β-Trefoil Lectin Domain.

M. Morales, Y. Luengo, J. Mancheño, 2016, Bioconjugate chemistry.

Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads®

O. Abián, C. Ortiz, R. Torres, 2006 .

Glutaraldehyde in Protein Immobilization

R. Fernández-Lafuente, N. Alonso-Morales, L. Betancor, 2006 .

Different derivatives of a lipase display different regioselectivity in the monohydrolysis of per-O-acetylated 1-O-substituted-β-galactopyranosides

R. Fernández-Lafuente, D. Rodrigues, J. Palomo, 2009 .

Purification, immobilization, and characterization of a specific lipase from Staphylococcus warneri EX17 by enzyme fractionating via adsorption on different hydrophobic supports

R. Rodrigues, R. Fernández-Lafuente, B. de Las Rivas, 2011, Biotechnology progress.

Recent Advances in the Industrial Enzymatic Synthesis of Semi-Synthetic βLactam Antibiotics

O. Abián, R. Torres, R. Fernández-Lafuente, 2005 .

Chemoenzymatic one-pot synthesis of cefazolin from cephalosporin C in fully aqueous medium, involving three consecutive biotransformations catalyzed by D-aminoacid oxidase, glutaryl acylase and penicillin G acylase

R. Fernández-Lafuente, M. Pregnolato, J. Guisán, 1997 .

Immobilization of a recombinant endo-1,5-arabinanase secreted by Aspergillus nidulans strain A773

M. Polizeli, A. Damasio, F. Segato, 2012 .

Immobilization-stabilization of alpha-chymotrypsin by covalent attachment to aldehyde-agarose gels.

C. Rosell, Á. Bastida, J. Guisán, 1991, Biotechnology and Bioengineering.

Partial purification and immobilization/stabilization on highly activated glyoxyl-agarose supports of different proteases from flavourzyme.

R. Fernández-Lafuente, M. Alaiz, C. Mateo, 2007, Journal of agricultural and food chemistry.

Mixed ion exchange supports as useful ion exchangers for protein purification: purification of penicillin G acylase from Escherichia coli.

R. Fernández-Lafuente, J. Hermoso, P. Batalla, 2007, Biomacromolecules.

Immobilization of Yarrowia lipolytica Lipase—a Comparison of Stability of Physical Adsorption and Covalent Attachment Techniques

A. G. Cunha, J. Bevilaqua, D. Freire, 2008, Applied biochemistry and biotechnology.

Immobilisation and stabilisation of β-galactosidase from Kluyveromyces lactis using a glyoxyl support

C. Bernal, M. Marciello, G. Fernandez-Lorente, 2013 .

Biocatalyst engineering of Thermomyces Lanuginosus lipase adsorbed on hydrophobic supports: Modulation of enzyme properties for ethanolysis of oil in solvent-free systems.

C. Farinas, J. Rocha-Martín, P. Tardioli, 2019, Journal of biotechnology.

Covalent immobilization-stabilization of β-1,4-endoxylanases from Trichoderma reesei: Production of xylooligosaccharides

M. F. Vieira, J. Rocha-Martín, M. Romero-Fernández, 2018 .

Production of xylo-oligosaccharides by immobilized-stabilized derivatives of endo-xylanase from Streptomyces halstedii

R. Monti, A. I. Ruiz-Matute, G. Fernandez-Lorente, 2013 .

Reversible Immobilization of Glucoamylase by Ionic Adsorption on Sepabeads Coated with Polyethyleneimine

C. Ortiz, R. Torres, R. Fernández-Lafuente, 2004, Biotechnology progress (Print).

A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports

Bastida, R. Fernández-Lafuente, P. Sabuquillo, 1998, Biotechnology and bioengineering.

Immobilization of peroxidase glycoprotein on gold electrodes modified with mixed epoxy-boronic Acid monolayers.

Marisela Vélez, Lo Gorton, Irina Gazaryan, 2002, Journal of the American Chemical Society.

Glycosylation of polyphenolic compounds: Design of a self-sufficient biocatalyst by co-immobilization of a glycosyltransferase, a sucrose synthase and the cofactor UDP.

J. Rocha-Martín, M. Romero-Fernández, J. Guisán, 2023, International journal of biological macromolecules.

Stabilization of an Amylase from Neurospora crassa by Immobilization on Highly Activated Supports

R. Monti, R. Fernández-Lafuente, B. Pessela, 2008 .

Enzymatic transesterification in a solvent-free system: synthesis of sn-2 docosahexaenoyl monoacylglycerol

J. Rocha-Martín, P. Luna, G. Fernandez-Lorente, 2018 .

Synthesis of sn-2 docosahexaenoyl monoacylglycerol by mild enzymatic transesterification of docosahexaenoic acid ethyl ester and glycerol in a solvent-free system

P. Luna, G. Fernandez-Lorente, J. Guisán, 2016 .

FOOD SCIENCE & TECHNOLOGY | SHORT COMMUNICATION Synthesis of sn-2 docosahexaenoyl monoacylglycerol by mild enzymatic transesterification of docosahexaenoic acid ethyl ester and glycerol in a solvent-free system

P. Luna, G. Fernandez-Lorente, J. Guisán, 2016 .