J. Cremata

发表

Structural characterization of N-linked oligosaccharides on monoclonal antibody Nimotuzumab through process development.

P. Rudd, D. Harvey, L. Calvo, 2012, Biologicals : journal of the International Association of Biological Standardization.

Variation in N-linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast Pichia pastoris.

R. Montesino, J. Cremata, R. García, 1998, Protein expression and purification.

Efficient expression and secretion of recombinant alpha amylase in Pichia pastoris using two different signal sequences

R. Montesino, J. Cremata, E. Paifer, 1994, Yeast.

An integral approach towards a practical application for a plant-made monoclonal antibody in vaccine purification.

C. Borroto, M. Pujol, M. Ayala, 2005, Vaccine.

Characterization of protein glycoforms with N‐linked neutral and phosphorylated oligosaccharides: studies on the glycosylation of endoglucanase 1 (Cel7B) from Trichoderma reesei

J. Ståhlberg, K. Benkestock, R. Montesino, 2001, Biotechnology and applied biochemistry.

The N-glycosylation of classical swine fever virus E2 glycoprotein extracellular domain expressed in the milk of goat.

R. Dwek, P. Rudd, D. Harvey, 2010, Archives of biochemistry and biophysics.

Monosialylated biantennary N-glycoforms containing GalNAc-GlcNAc antennae predominate when human EPO is expressed in goat milk.

R. Dwek, P. Rudd, D. Harvey, 2008, Archives of biochemistry and biophysics.

The goat mammary glandular epithelial (GMGE) cell line promotes polyfucosylation and N,N'-diacetyllactosediaminylation of N-glycans linked to recombinant human erythropoietin.

R. Dwek, P. Rudd, D. Harvey, 2007, Archives of biochemistry and biophysics.

Analytical strategies to investigate plant N-glycan profiles in the context of plant-made pharmaceuticals.

R. Dwek, P. Lerouge, P. Rudd, 2006, Current opinion in structural biology.

Plant N-glycan profiling of minute amounts of material.

R. Dwek, P. Lerouge, A. Triguero, 2008, Analytical biochemistry.

Chemical and enzymatic N-glycan release comparison for N-glycan profiling of monoclonal antibodies expressed in plants.

R. Dwek, P. Lerouge, A. Triguero, 2010, Analytical biochemistry.

Influence of culture conditions on the N‐glycosylation of a monoclonal antibody specific for recombinant hepatitis B surface antigen

R. Montesino, J. Cremata, Gleysin Cabrera, 2005, Biotechnology and applied biochemistry.

A NEW POLYSACCHARIDE ISOLATED FROM HIGH VISCOSITY FINAL MOLASSES STRUCTURAL STUDY

L., R. Orozco, J. Cremata, 2007 .

Dextranase (alpha-1,6 glucan-6-glucanohydrolase) from Penicillium minioluteum expressed in Pichia pastoris: two host cells with minor differences in N-glycosylation.

T. Takao, Y. Shimonishi, L. Betancourt, 2001, FEMS yeast research.

Cellobiose quinone oxidoreductase from the white rot fungus Phanerochaete chrysosporium is produced by intracellular proteolysis of cellobiose dehydrogenase.

G. Pettersson, G. Henriksson, I. Szabó, 2002, Biochimica et biophysica acta.

Rapid and selective production of hybridoma cells secreting novel monoclonal antibodies based on short-term immunization

C. Borroto, M. Pujol, J. Cremata, 2006 .

Production of plantibodies in Nicotiana plants.

M. Pujol, M. Ayala, J. Gavilondo, 2009, Methods in molecular biology.

Purification and characterization of an invertase from Candida utilis: comparison with natural and recombinant yeast invertases.

L. Rodríguez, J. Diaz, J. Cremata, 1997, Journal of biotechnology.

Isolation, purification and characterization of hyaluronan from human umbilical cord residues

J. Kennedy, C. Pérez, J. Cremata, 2005 .

High expression level of recombinant human erythropoietin in the milk of non-transgenic goats.

P. A. Zamora, J. Cremata, M. P. Rodríguez, 2006, Journal of biotechnology.

High level expression of the B. microplus Bm86 antigen in the yeast Pichia pastoris forming highly immunogenic particles for cattle.

M. Penichet, R. Bringas, J. Fuente, 1994, Journal of biotechnology.

The cattle tick antigen, Bm95, expressed in Pichia pastoris contains short chains of N- and O-glycans.

T. Pons, A. Triguero, V. Huerta, 2004, Archives of biochemistry and biophysics.

Biochemical characterization of the recombinant Boophilus microplus Bm86 antigen expressed by transformed Pichia pastoris cells

V. Besada, V. Falcón, R. Montesino, 1996, Biotechnology and applied biochemistry.

On the isolation of immunostimulatory active acemannan from Aloe barbadensis

G. Gerwig, J. Cremata, Y. Támbara, 2012 .

Rapid and sensitive anthrone-sulfuric acid assay in microplate format to quantify carbohydrate in biopharmaceutical products: method development and validation.

Julio C. Sánchez, A. Leyva, J. Cremata, 2008, Biologicals : journal of the International Association of Biological Standardization.

Electron impact mass spectra of per-O-acetyl derivatives of 4′-substituted glycopyranosylphenylamines

A. Nuñez, J. Cremata, R. León, 1988 .

Physicochemical and biological characterization of 1E10 Anti-Idiotype vaccine

V. Besada, Y. Machado, M. Himly, 2011, BMC biotechnology.

Relationships between the N‐glycan structures and biological activities of recombinant human erythropoietins produced using different culture conditions and purification procedures

R. Wait, Robin Wait, J. Cremata, 2003, British journal of haematology.

Differential in vitro and in vivo glycosylation of human erythropoietin expressed in adenovirally transduced mouse mammary epithelial cells.

J. Cremata, M. P. Rodríguez, J. Toledo, 2005, Biochimica et biophysica acta.

Characterization of monosaccharide mixtures by low-energy electron impact mass spectrometry

A. Nuñez, J. Cremata, E. Díaz, 1989 .

Differential N-glycosylation of a monoclonal antibody expressed in tobacco leaves with and without endoplasmic reticulum retention signal apparently induces similar in vivo stability in mice.

M. Wormald, A. Triguero, Meilyn Rodríguez, 2011, Plant biotechnology journal.

Knowing the glycosylation machinery of mammary gland cells : Potential and drawbacks for the expression of proteins of pharmaceutical interest

R. Montesino, J. Cremata, M. P. Rodríguez, 2009 .

Expression and characterization of an anti‐(hepatitis B surface antigen) glycosylated mouse antibody in transgenic tobacco (Nicotiana tabacum) plants and its use in the immunopurification of its target antigen

M. Ayala, J. Gavilondo, R. Páez, 2003, Biotechnology and applied biochemistry.

Cloning and characterization of a cDNA encoding a cellobiose dehydrogenase from the white rot fungus Phanerochaete chrysosporium

C. Divne, J. Ståhlberg, G. Pettersson, 1995, FEBS letters.

New procedure for the production of biopharmaceutical proteins in the milk of non-transgenic animals

R. Montesino, J. Cremata, M. P. Rodríguez, 2005 .

Highly protective E2-CSFV vaccine candidate produced in the mammary gland of adenoviral transduced goats.

P. Alfonso, L. Ganges, R. Montesino, 2008, Journal of biotechnology.

Cloning of the Penicillium minioluteum gene encoding dextranase and its expression in Pichia pastoris

T. Pons, J. Cremata, J. Delgado, 1996, Yeast.

N-glycosylation pattern of E2 glycoprotein from classical swine fever virus.

R. Dwek, P. Rudd, D. Harvey, 2009, Journal of proteome research.

Rapid and sensitive anthrone e sulfuric acid assay in microplate format to quantify carbohydrate in biopharmaceutical products : Method development and validation

Julio C. Sánchez, A. Leyva, J. Cremata, 2022 .

Characterization of the oligosaccharides assembled on the Pichia pastoris-expressed recombinant aspartic protease.

M. Nimtz, V. Falcón, R. Montesino, 1999, Glycobiology.

Invertase secretion in Hansenula polymorpha under the AOX1 promoter from Pichia pastoris

L. Rodríguez, J. Cremata, L. Herrera, 1996, Yeast.

Glycan Engineering in Transgenic Plants

P. Lerouge, M. Bardor, J. Cremata, 2010 .

Plant-derived mouse IgG monoclonal antibody fused to KDEL endoplasmic reticulum-retention signal is N-glycosylated homogeneously throughout the plant with mostly high-mannose-type N-glycans.

A. Triguero, J. Cremata, Gleysin Cabrera, 2005, Plant biotechnology journal.

Two-dimensional mapping of 8-amine-1,3,6-naphthalene trisulfonic acid derivatives of N-linked neutral and sialyloligosaccharides.

R. Montesino, J. Cremata, O. Quintero, 1998, Analytical biochemistry.

Glycosylation profiling of heterologous proteins.

R. Montesino, J. Cremata, R. García, 1998, Methods in molecular biology.