N. Nakashima

发表

Conditional gene silencing of multiple genes with antisense RNAs and generation of a mutator strain of Escherichia coli

Tomohiro Tamura, Nobutaka Nakashima, N. Nakashima, 2009, Nucleic acids research.

Genetic and biochemical characterization of the dioxygenase involved in lateral dioxygenation of dibenzofuran from Rhodococcus opacus strain SAO101

Y. Kamagata, W. Kitagawa, N. Kimura, 2006, Applied Microbiology and Biotechnology.

Actinomycetes as host cells for production of recombinant proteins

N. Nakashima, T. Tamura, Y. Mitani, 2005, Microbial cell factories.

Comparative Genome Analysis of Three Komagataeibacter Strains Used for Practical Production of Nata-de-Coco

K. Kimura, N. Nakashima, Koji Ishiya, 2022, Frontiers in Microbiology.

Production of acetoin from hydrothermally pretreated oil mesocarp fiber using metabolically engineered Escherichia coli in a bioreactor system.

M. Hassan, Hironaga Akita, S. Fujimoto, 2017, Bioresource technology.

Cell-free protein synthesis using cell extract of Pseudomonas fluorescens and CspA promoter.

N. Nakashima, T. Tamura, 2004, Biochemical and biophysical research communications.

Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential

Hironaga Akita, T. Hoshino, Mohd Zulkhairi Mohd Yusoff, 2016, SpringerPlus.

Scaffold size-dependent effect on the enhanced uptake of antibiotics and other compounds by Escherichia coli and Pseudomonas aeruginosa

Shotaro Ayukawa, Y. Yasutake, N. Nakashima, 2022, Scientific reports.

Isolation and Characterization of a Rolling-Circle-Type Plasmid from Rhodococcus erythropolis and Application of the Plasmid to Multiple-Recombinant-Protein Expression

N. Nakashima, T. Tamura, 2004, Applied and Environmental Microbiology.

MazF activation causes ACA sequence-independent and selective alterations in RNA levels in Escherichia coli

H. Kondo, Shotaro Ayukawa, Yuya Nakamura, 2019, Archives of Microbiology.

Identification of a methanol-inducible promoter from Rhodococcus erythropolis PR4 and its use as an expression vector.

Hea-Yeon Yun, N. Tamura, N. Nakashima, 2012, Journal of bioscience and bioengineering.

MazF activation causes ACA sequence-independent and selective alterations in RNA levels in Escherichia coli

H. Kondo, Shotaro Ayukawa, Yuya Nakamura, 2019, Archives of Microbiology.

Isolation and characterization of the Rhodococcus opacus thiostrepton-inducible genes tipAL and tipAS: application for recombinant protein expression in Rhodococcus.

N. Tamura, N. Nakashima, T. Tamura, 2004, FEMS microbiology letters.

A novel system for expressing recombinant proteins over a wide temperature range from 4 to 35°C

N. Nakashima, T. Tamura, 2004 .

Molecular cloning and characterization of two YGL039w genes encoding broad specificity NADPH-dependent aldehyde reductases from Kluyveromyces marxianus strain DMB1.

Masahiro Watanabe, Hironaga Akita, T. Hoshino, 2015, FEMS microbiology letters.

Characterization of the Kluyveromyces marxianus strain DMB1 YGL157w gene product as a broad specificity NADPH-dependent aldehyde reductase

Masahiro Watanabe, Hironaga Akita, T. Hoshino, 2015, AMB Express.

Concurrent Growth Rate and Transcript Analyses Reveal Essential Gene Stringency in Escherichia coli

L. Good, J. Stach, S. Goh, 2009, PloS one.

Bacterial Cellular Engineering by Genome Editing and Gene Silencing

K. Miyazaki, N. Nakashima, 2014, International journal of molecular sciences.

Comparative Genome Analysis Reveals Accumulation of Single-Nucleotide Repeats in Pathogenic Escherichia Lineages

N. Nakashima, Koji Ishiya, 2022, Current issues in molecular biology.

Production of d-lactate using a pyruvate-producing Escherichia coli strain

Hironaga Akita, T. Hoshino, N. Nakashima, 2017, Bioscience, biotechnology, and biochemistry.

Pyruvate production using engineered Escherichia coli

Tamotsu Hoshino, Hironaga Akita, T. Hoshino, 2016, AMB Express.

A Vector Library for Silencing Central Carbon Metabolism Genes with Antisense RNAs in Escherichia coli

Katsunori Yoshikawa, Satoshi Ohno, Hiroshi Shimizu, 2013, Applied and Environmental Microbiology.

Pyruvate production using engineered Escherichia coli

Hironaga Akita, T. Hoshino, N. Nakashima, 2016, AMB Express.

Establishment of a multi-species biofilm model and metatranscriptomic analysis of biofilm and planktonic cell communities

K. Kurokawa, Nozomi Yamamoto, Hiroshi Mori, 2016, Applied Microbiology and Biotechnology.

Scaffold size-dependent effect on the enhanced uptake of antibiotics and other compounds by Escherichia coli and Pseudomonas aeruginosa

Shotaro Ayukawa, Y. Yasutake, N. Nakashima, 2022, Scientific Reports.

Identification and characterization of Burkholderia multivorans CCA53

Hironaga Akita, T. Hoshino, N. Nakashima, 2017, BMC Research Notes.

Counterselection method based on conditional silencing of antitoxin genes in Escherichia coli.

K. Miyazaki, N. Nakashima, M. Tsukuda, 2015, Journal of bioscience and bioengineering.

Instructions for use Title Conditional gene silencing of multiple genes with antisense RNAs and generation of a mutator strain of Escherichia coli

N. Nakashima, T. Tamura, 2018 .

Paired termini stabilize antisense RNAs and enhance conditional gene silencing in Escherichia coli

Tomohiro Tamura, L. Good, Nobutaka Nakashima, 2006, Nucleic acids research.

Bacterial production of isobutanol without expensive reagents

Hironaga Akita, T. Hoshino, N. Nakashima, 2014, Applied Microbiology and Biotechnology.

Advances in the Development of Genetic Tools for the Genus Rhodococcus

K. Sallam, N. Nakashima, T. Tamura, 2006 .

Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin.

Tamotsu Hoshino, Hironaga Akita, T. Hoshino, 2014, Metabolic engineering.

Identification and characterization of Burkholderia multivorans CCA53

Hironaga Akita, M. Yusoff, T. Hoshino, 2017, BMC Research Notes.

Characterization of the Kluyveromyces marxianus strain DMB1 YGL157w gene product as a broad specificity NADPH-dependent aldehyde reductase

Masahiro Watanabe, Hironaga Akita, T. Hoshino, 2015, AMB Express.

A novel system for expressing recombinant proteins over a wide temperature range from 4 to 35 degrees C.

N. Nakashima, T. Tamura, 2004, Biotechnology and bioengineering.

A new carbon catabolite repression mutation of Escherichia coli, mlc∗, and its use for producing isobutanol.

N. Nakashima, T. Tamura, 2012, Journal of bioscience and bioengineering.

Genome Sequence of Rhodococcus erythropolis Type Strain JCM 3201

S. Aburatani, W. Kitagawa, Keitaro Yoshida, 2019, Microbiology Resource Announcements.

Actinomycetes as host cells for production of recombinant proteins

N. Nakashima, T. Tamura, Y. Mitani, 2005, Microbial cell factories.

Gene silencing in Escherichia coli using antisense RNAs expressed from doxycycline‐inducible vectors

N. Nakashima, T. Tamura, 2013, Letters in applied microbiology.

A Vector Library for Silencing Central Carbon Metabolism Genes with Antisense RNAs in Escherichia coli

H. Shimizu, S. Ohno, N. Nakashima, 2013, Applied and Environmental Microbiology.

Identification of a methanol-inducible promoter from Rhodococcus erythropolis PR4 and its use as an expression vector.

N. Tamura, N. Nakashima, T. Tamura, 2012, Journal of bioscience and bioengineering.