T. Takita

发表

Optimization of reaction condition of recombinase polymerase amplification to detect SARS-CoV-2 DNA and RNA using a statistical method

E. Arikawa, S. Fujiwara, K. Yasukawa, 2021, Biochemical and Biophysical Research Communications.

Substrate-induced conformational changes of the truncated catalytic domain of Geobacillus stearothermophilus lysyl-tRNA synthetase as examined by fluorescence.

K. Inouye, T. Takita, Yoshihiro Saruwatari, 2008, Biochimica et biophysica acta.

Extracellular production of recombinant thermolysin expressed in Escherichia coli, and its purification and enzymatic characterization.

K. Inouye, Masayuki Kusano, Y. Hashida, 2006, Protein expression and purification.

Solvent engineering studies on recombinase polymerase amplification.

C. O’Sullivan, S. Fujiwara, K. Yasukawa, 2020, Journal of bioscience and bioengineering.

Improvement of Moloney murine leukemia virus reverse transcriptase thermostability by introducing a disulfide bridge in the ribonuclease H region.

Kei Iida, K. Yasukawa, K. Kojima, 2021, Protein engineering, design & selection : PEDS.

Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system

K. Yasukawa, K. Kojima, Masaaki Ito, 2017, Bioscience, biotechnology, and biochemistry.

Biodegradation of polychlorinated dibenzo-p-dioxins by recombinant yeast expressing rat CYP1A subfamily.

K. Inouye, T. Sakaki, Raku Shinkyo, 2002, Archives of biochemistry and biophysics.

Sequential hydroxylation of vitamin D2 by a genetically engineered CYP105A1.

K. Yasuda, K. Yasukawa, S. Ikushiro, 2016, Biochemical and biophysical research communications.

Effects of neutral salts and pH on the activity and stability of human RNase H2

K. Yasukawa, K. Kojima, R. Crouch, 2017, Journal of biochemistry.

Crystal structure of Grimontia hollisae collagenase provides insights into its novel substrate specificity toward collagen

B. Mikami, K. Yasukawa, S. Hattori, 2022, The Journal of biological chemistry.

Increase in the thermostability of GH11 xylanase XynJ from Bacillus sp. strain 41M-1 using site saturation mutagenesis.

B. Mikami, R. Yatsunami, Satoshi Nakamura, 2019, Enzyme and microbial technology.

Metabolism of polychlorinated dibenzo-p-dioxins (PCDDs) by human cytochrome P450-dependent monooxygenase systems.

K. Inouye, T. Sakaki, Raku Shinkyo, 2002, Journal of agricultural and food chemistry.

Lysyl-tRNA Synthetase of Bacillus stearothermophilus Molecular Cloning and Expression of the Gene

K. Inouye, B. Tonomura, K. Soda, 2000, Bioscience, biotechnology, and biochemistry.

Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase.

H. Okano, S. Fujiwara, K. Yasukawa, 2017, Enzyme and microbial technology.

Lysyl-tRNA synthetase from Bacillus stearothermophilus. Formation and isolation of an enzyme-lysyladenylate complex and its analogue.

T. Sukata, K. Inouye, B. Tonomura, 1997, Journal of biochemistry.

Insight into the mechanism of thermostabilization of GH10 xylanase from Bacillus sp. strain TAR-1 by the mutation of S92 to E.

B. Mikami, R. Yatsunami, Satoshi Nakamura, 2021, Bioscience, biotechnology, and biochemistry.

Modified uvsY by N-terminal hexahistidine tag addition enhances efficiency of recombinase polymerase amplification to detect SARS-CoV-2 DNA

S. Fujiwara, K. Yasukawa, Wakao Fukuda, 2022, Molecular biology reports.

Characterization of six recombinant human RNase H2 bearing Aicardi-Goutiéres syndrome causing mutations.

K. Yasukawa, K. Kojima, R. Crouch, 2019, Journal of biochemistry.

Generation of 2,3,7,8-TCDD-metabolizing enzyme by modifying rat CYP1A1 through site-directed mutagenesis.

K. Inouye, T. Sakaki, Raku Shinkyo, 2003, Biochemical and biophysical research communications.

Deodorization of soybean proteins by enzymatic and physicochemical treatments.

K. Inouye, T. Takita, Misa Shiihara, 2002, Journal of Agricultural and Food Chemistry.

Next-generation sequencing-based analysis of reverse transcriptase fidelity.

Kei Iida, H. Okano, S. Fujiwara, 2017, Biochemical and biophysical research communications.

Kinetic analysis of inhibition of α-glucosidase by leaf powder from Morus australis and its component iminosugars

K. Yasukawa, Toshiyuki Kimura, K. Kojima, 2020, Bioscience, biotechnology, and biochemistry.

Inhibition of α-amylase and α-glucosidase by Morus australis fruit extract and its components iminosugar, anthocyanin, and glucose.

Toshiyuki Kimura, K. Yasukawa, T. Takita, 2022, Journal of food science.

Protein engineering of CYP105s for their industrial uses.

K. Yasuda, H. Sugimoto, K. Yasukawa, 2018, Biochimica et biophysica acta. Proteins and proteomics.

Production of an active form of vitamin D2 by genetically engineered CYP105A1.

K. Yasuda, H. Sugimoto, K. Yasukawa, 2017, Biochemical and biophysical research communications.

Metabolism of non-steroidal anti-inflammatory drugs (NSAIDs) by Streptomyces griseolus CYP105A1 and its variants.

K. Yasuda, K. Yasukawa, S. Ikushiro, 2022, Drug metabolism and pharmacokinetics.

Kinetic Analysis of the Digestion of Bovine Type I Collagen Telopeptides with Porcine Pepsin.

K. Yasukawa, S. Hattori, Keisuke Tanaka, 2016, Journal of food science.

Insight into the collagen-degrading activity of a serine protease in the latex of Ficus carica cultivar Masui Dauphine.

K. Yasukawa, K. Kojima, Toru Takahashi, 2021, Bioscience, biotechnology, and biochemistry.

The roles of histidine and tyrosine residues in the active site of collagenase in Grimontia hollisae.

K. Yasukawa, S. Hattori, Keisuke Tanaka, 2020, Journal of biochemistry.

Comparative studies on the activities of collagenases from Grimontia hollisae and Clostridium hystoliticum in the hydrolysis of synthetic substrates

K. Yasukawa, S. Hattori, Keisuke Tanaka, 2018, Journal of biochemistry.

Development of immersion vaccine for bacterial cold-water disease in ayu Plecoglossus altivelis

K. Yasukawa, S. Hattori, Keisuke Tanaka, 2017, Bioscience, biotechnology, and biochemistry.

Increase in the thermostability of Bacillus sp. strain TAR-1 xylanase using a site saturation mutagenesis library

R. Yatsunami, Satoshi Nakamura, K. Yasukawa, 2018, Bioscience, biotechnology, and biochemistry.

Two crystal structures of lysyl-tRNA synthetase from Bacillus stearothermophilus in complex with lysyladenylate-like compounds: insights into the irreversible formation of the enzyme-bound adenylate of L-lysine hydroxamate.

B. Mikami, K. Inouye, K. Yasukawa, 2009, Journal of biochemistry.

Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability.

K. Inouye, Masashi Minoda, K. Yasukawa, 2008, Biochimica et biophysica acta.

Recombinase polymerase amplification using novel thermostable strand-displacing DNA polymerases from Aeribacillus pallidus and Geobacillus zalihae.

S. Fujiwara, K. Yasukawa, Wakao Fukuda, 2023, Journal of bioscience and bioengineering.

Modified uvsY by N-terminal hexahistidine tag addition enhances efficiency of recombinase polymerase amplification to detect SARS-CoV-2 DNA

S. Fujiwara, K. Yasukawa, Wakao Fukuda, 2021, Molecular Biology Reports.

High sensitive one-step RT-PCR using MMLV reverse transcriptase, DNA polymerase with reverse transcriptase activity, and DNA/RNA helicase.

H. Okano, S. Fujiwara, K. Yasukawa, 2017, Biochemical and biophysical research communications.

Comparison of sensitivity and rapidness of PCR, recombinase polymerase amplification, and RNA-specific amplification for detection of Rice yellow mottle virus

K. Yasukawa, K. Kojima, Kevin Maafu Juma, 2021, Journal of Biological Macromolecules.

Construction and characterization of ribonuclease H2 knockout NIH3T3 cells

K. Yasukawa, K. Kojima, T. Takita, 2018, Journal of biochemistry.

The mutation of Thr315 to Asn of GH10 xylanase XynR increases the alkaliphily but decreases the alkaline resistance.

R. Yatsunami, Satoshi Nakamura, K. Yasukawa, 2021, Bioscience, biotechnology, and biochemistry.

Lysyl-tRNA synthetase from Bacillus stearothermophilus. Purification, and fluorometric and kinetic analysis of the binding of substrates, L-lysine and ATP.

K. Inouye, B. Tonomura, H. Ito, 1996, Journal of biochemistry.

Lysyl-tRNA synthetase from Bacillus stearothermophilus: the Trp314 residue is shielded in a non-polar environment and is responsible for the fluorescence changes observed in the amino acid activation reaction.

K. Inouye, B. Tonomura, T. Takita, 2003, Journal of molecular biology.

Transition State Stabilization by the N-terminal Anticodon-binding Domain of Lysyl-tRNA Synthetase* 210

K. Inouye, Kuniyo Inouye, T. Takita, 2002, The Journal of Biological Chemistry.

Further increase in thermostability of Moloney murine leukemia virus reverse transcriptase by mutational combination

K. Yasukawa, K. Kojima, C. Leucht, 2017, Protein engineering, design & selection : PEDS.

Construction and characterization of ribonuclease H2 C subunit-knockout NIH3T3 cells.

K. Yasukawa, T. Takita, Mako Kandabashi, 2023, Bioscience, biotechnology, and biochemistry.

Val143 of human ribonuclease H2 is not critical for, but plays a role in determining catalytic activity and substrate specificity

K. Yasukawa, K. Kojima, R. Crouch, 2020, PloS one.

Analysis of ribonucleotide content in the genomic DNA of ribonuclease H2 A subunit (RH2A)-knockout NIH3T3 cells after transient expression of wild-type RH2A or RH2A variants with an Aicardi-Goutières syndrome-causing mutation.

K. Yasukawa, T. Takita, Mako Kandabashi, 2022, Journal of Biochemistry (Tokyo).

Accurate fidelity analysis of the reverse transcriptase by a modified next-generation sequencing.

H. Okano, S. Fujiwara, K. Yasukawa, 2018, Enzyme and microbial technology.

High sensitive RNA detection by one-step RT-PCR using the genetically engineered variant of DNA polymerase with reverse transcriptase activity from hyperthermophilies.

H. Okano, S. Fujiwara, K. Yasukawa, 2017, Journal of bioscience and bioengineering.

Insights into the catalytic mechanism of Grimontia hollisae collagenase through structural and mutational analyses

Keisuke Tanaka, T. Takita, K. Mizutani, 2023, FEBS letters.

Adenosine triphosphate induces amorphous aggregation of amyloid β by increasing Aβ dynamics

K. Yoshimune, T. Komoriya, T. Takita, 2024, Scientific Reports.

Achieving unprecedented stability in lyophilized recombinase polymerase amplification with thermostable pyruvate kinase from Thermotoga maritima.

I. Yanagihara, Kevin Maafu Juma, T. Takita, 2024, Journal of bioscience and bioengineering.

Use of human Caco-2 cells and HPAE-PAD for α-glucosidase assay.

K. Yasukawa, T. Takita, Haruna Yano, 2024, Journal of pharmacological and toxicological methods.