E. Mihara

发表

Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System.

J. Takagi, T. Nogi, N. Yasui, 2017, Methods in molecular biology.

A rapid screening method for cell lines producing singly-tagged recombinant proteins using the "TARGET tag" system.

J. Takagi, I. Fujii, E. Mihara, 2010, Journal of proteomics.

Structure and biological function of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase

Junichi Takagi, Hirofumi Ueda, Emiko Mihara, 2016, Scientific Reports.

Crystal structure of autotaxin and insight into GPCR activation by lipid mediators

H. Nishimasu, O. Nureki, J. Takagi, 2011, Nature Structural &Molecular Biology.

Crystal structure of Enpp1, an extracellular glycoprotein involved in bone mineralization and insulin signaling

H. Nishimasu, O. Nureki, J. Takagi, 2012, Proceedings of the National Academy of Sciences.

Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin

J. Takagi, Toshiro Sato, Hideki Yamamoto, 2016, eLife.

Functional role of the Frizzled linker domain in the Wnt signaling pathway

Chanhee Kang, E. Mihara, I. Bang, 2022, Communications Biology.

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6.

H. Nishimasu, O. Nureki, J. Takagi, 2014, Acta crystallographica. Section F, Structural biology communications.

Hybrid in vitro/in silico analysis of low‐affinity protein–protein interactions that regulate signal transduction by Sema6D

T. Yamane, M. Ikeguchi, S. Akashi, 2022, Protein science : a publication of the Protein Society.

Mathematical modeling and analysis of the pathway network consisting of symmetrical complexes with N monomers , like the activation of MMP 2

M. Horikoshi, M. Feinberg, Y. Goshima, 2018 .

Anti–USAG-1 therapy for tooth regeneration through enhanced BMP signaling

Y. Tabata, R. Uozumi, K. Bessho, 2021, Science Advances.

Macrocyclic peptides that inhibit Wnt signalling via interaction with Wnt3a

J. Takagi, H. Suga, T. Passioura, 2020, RSC chemical biology.

Lasso-grafting of macrocyclic peptide pharmacophores yields multi-functional proteins

J. Takagi, Kunio Matsumoto, H. Suga, 2020, Nature Communications.

Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V

J. Takagi, N. Taniguchi, Yasuhiko Kizuka, 2018, Nature Communications.

Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain

K. Akashi, K. Takenaka, H. Kishima, 2022, Science Translational Medicine.

Conformational Freedom of the LRP6 Ectodomain Is Regulated by N-glycosylation and the Binding of the Wnt Antagonist Dkk1.

J. Takagi, N. Miyazaki, K. Iwasaki, 2017, Cell reports.

Structural basis for semaphorin signalling through the plexin receptor

J. Takagi, Y. Goshima, S. Uchiyama, 2010, Nature.

De novo peptide grafting to a self-assembling nanocapsule yields a hepatocyte growth factor receptor agonist

D. Hilvert, Kunio Matsumoto, H. Suga, 2021, iScience.

Crystal structure of a mammalian Wnt–frizzled complex

J. Takagi, E. Mihara, T. Arimori, 2019, Nature Structural & Molecular Biology.

An anti-peptide monoclonal antibody recognizing the tobacco etch virus protease-cleavage sequence and its application to a tandem tagging system.

Katsu Takahashi, J. Takagi, Y. Kato, 2018, Protein expression and purification.

Mechanistic insights into ectodomain shedding: susceptibility of CADM1 adhesion molecule is determined by alternative splicing and O-glycosylation

A. Yoshimura, J. Takagi, Keiichi Homma, 2017, Scientific Reports.

Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions

Hiroki Sato, D. Voon, H. Suga, 2022, Nature Biomedical Engineering.

Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2

J. Takagi, Y. Matsuura, D. Motooka, 2021, Nature Communications.

Crystal structure of α5β1 integrin ectodomain: Atomic details of the fibronectin receptor

Y. Sugita, J. Takagi, T. Nogi, 2012, The Journal of cell biology.

Structural basis for amyloidogenic peptide recognition by sorLA

J. Takagi, Koichi Kato, M. Yagi-Utsumi, 2015, Nature Structural &Molecular Biology.

Structural mechanism of laminin recognition by integrin

K. Sekiguchi, C. Cabañas, J. Takagi, 2021, Nature Communications.

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1.

H. Nishimasu, O. Nureki, J. Takagi, 2012, Acta crystallographica. Section F, Structural biology and crystallization communications.

Crystal structure of a mammalian Wnt–frizzled complex

J. Takagi, E. Mihara, T. Arimori, 2019, Nature Structural & Molecular Biology.

Lasso-grafting of macrocyclic peptide pharmacophores yields multi-functional proteins

H. Suga, Yizhen Yin, E. Mihara, 2021, Nature Communications.

Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation.

Junichi Takagi, Emiko Mihara, J. Takagi, 2010, Journal of immunological methods.

Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin

J. Takagi, Toshiro Sato, Hideki Yamamoto, 2016, eLife.