A. Skerra

发表

An engineered IN-1 F(ab) fragment with improved affinity for the Nogo-A axonal growth inhibitor permits immunochemical detection and shows enhanced neutralizing activity.

M. Schwab, C. Bandtlow, M. Fiedler, 2002, Protein engineering.

Immobilization of histidine-tagged proteins on gold surfaces using chelator thioalkanes.

M. Liley, H. Vogel, A. Skerra, 1999, Biosensors & bioelectronics.

Regeneration of Auditory Nerve Following Complete Sectioning and Intrathecal Application of the IN-1 Antibody

A. Gharabaghi, M. Schwab, M. Samii, 2002, Acta Neurochirurgica.

Glutathione S‐transferase can be used as a C‐terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli

T. Tudyka, A. Skerra, 1997, Protein science : a publication of the Protein Society.

Bacterial expression of immunoglobulin fragments.

A. Skerra, 1993, Current opinion in immunology.

Neutrophil Gelatinase-Associated Lipocalin Expresses Antimicrobial Activity by Interfering with L -Norepinephrine-Mediated Bacterial Iron Acquisition (cid:1)

M. Miethke, A. Skerra, 2010 .

Efficient secretory production of proline/alanine/serine (PAS) biopolymers in Corynebacterium glutamicum yielding a monodisperse biological alternative to polyethylene glycol (PEG)

Y. Matsuda, A. Skerra, L. Friedrich, 2022, Microbial Cell Factories.

Intracellular expression of a cloned antibody fragment interferes with hepatitis B virus surface antigen secretion.

J. Wands, A. Skerra, J. zu Putlitz, 1999, Biochemical and biophysical research communications.

A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody Fab fragments.

A. Skerra, 1994, Gene.

Filter screening of antibody Fab fragments secreted from individual bacterial colonies: specific detection of antigen binding with a two-membrane system.

G. Winter, M. Dreher, A. Skerra, 1991, Analytical biochemistry.

Engineering of antibodies with a known three-dimensional structure.

R. Glockshuber, A. Plückthun, I. Pfitzinger, 1987, Cold Spring Harbor symposia on quantitative biology.

The Functional Expression of Antibody Fv Fragments in Ischhuchia coli: Improved Vectors and a Generally Applicable Purification Technique

A. Plückthun, I. Pfitzinger, A. Skerra, 1991, Bio/Technology.

Assembly of a functional immunoglobulin Fv fragment in Escherichia coli.

A. Plückthun, A. Skerra, 1988, Science.

Structural insights into the antigenicity of myelin oligodendrocyte glycoprotein

R. Huber, U. Jacob, H. Zander, 2003, Proceedings of the National Academy of Sciences of the United States of America.

Rapid expression cloning of human immunoglobulin Fab fragments for the analysis of antigen specificity of B cell lymphomas and anti-idiotype lymphoma vaccination.

A. Mackensen, A. Lindemann, H. Veelken, 1999, Journal of immunological methods.

Structural features of the McPC603 Fab fragment not defined in the X‐ray structure

R. Glockshuber, A. Plückthun, A. Skerra, 1990, FEBS letters.

The periplasmic E. coli chaperone Skp is a trimer in solution: biophysical and preliminary crystallographic characterization

K. Hadian, A. Skerra, M. Fogarasi, 2004, Biological chemistry.

Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity.

A Skerra, A. Skerra, 1992, Nucleic acids research.

Single-step purification of a bacterially expressed antibody Fv fragment by immobilized metal affinity chromatography in the presence of betaine.

L. Essen, A. Skerra, 1993, Journal of chromatography. A.

Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli.

A. Skerra, 1994, Gene.

The rational construction of an antibody against cystatin: lessons from the crystal structure of an artificial Fab fragment.

A. Skerra, W. Schiweck, 1997, Journal of molecular biology.

Sequence analysis and bacterial production of the anti‐c‐myc antibody 9E10: the VH domain has an extended CDR‐H3 and exhibits unusual solubility

A. Skerra, W. Schiweck, B. Buxbaum, 1997, FEBS letters.

Engineered Fv Fragments as a Tool for the One-Step Purification of Integral Multisubunit Membrane Protein Complexes

H. Michel, C. Ostermeier, B. Ludwig, 1995, Bio/Technology.

Regeneration of Lesioned Corticospinal Tract Fibers in the Adult Rat Induced by a Recombinant, Humanized IN-1 Antibody Fragment

M. Schwab, M. Fiedler, C. Brösamle, 2000, The Journal of Neuroscience.

Use of an albumin-binding domain for the selective immobilisation of recombinant capture antibody fragments on ELISA plates.

A. Skerra, T. König, 1998, Journal of immunological methods.

Expression of functional antibody Fv and Fab fragments in Escherichia coli.

A. Plückthun, A Plückthun, A Skerra, 1989, Methods in enzymology.

Fermenter production of an artificial fab fragment, rationally designed for the antigen cystatin, and its optimized crystallization through constant domain shuffling

A. Skerra, W. Schiweck, 1995, Proteins.

Secretion and in vivo folding of the Fab fragment of the antibody McPC603 in Escherichia coli: influence of disulphides and cis-prolines.

A. Plückthun, A. Skerra, 1991, Protein engineering.

Colony assays for antibody fragments expressed in bacteria.

C. Milstein, E. Gherardi, M. Dreher, 1991, Journal of immunological methods.

Retrograde Regulation of Growth-Associated Gene Expression in Adult Rat Purkinje Cells by Myelin-Associated Neurite Growth Inhibitory Proteins

P. Strata, F. Rossi, M. Schwab, 1998, The Journal of Neuroscience.

The Escherichia coli-derived Fab fragment of the IgM/kappa antibody IN-1 recognizes and neutralizes myelin-associated inhibitors of neurite growth.

M. Schwab, C. Bandtlow, H. Tai, 1996, European journal of biochemistry.

A novel method for the N-terminal modification of native proteins.

F. Schmidtchen, A. Skerra, C. Seitz, 2004, Bioconjugate chemistry.

Investigation of a tetracycline-regulated phage display system.

W. Höhne, G. Zahn, A. Skerra, 1999, Protein engineering.

Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold.

A. Skerra, G. Beste, F. S. Schmidt, 1999, Proceedings of the National Academy of Sciences of the United States of America.

Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification.

S. Voss, A. Skerra, A. Skerra, 1997, Protein engineering.

One-step affinity purification of bacterially produced proteins by means of the "Strep tag" and immobilized recombinant core streptavidin.

A. Skerra, T G Schmidt, A Skerra, 1994, Journal of chromatography. A.

Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin.

R. Frank, A. Skerra, T G Schmidt, 1996, Journal of molecular biology.

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment.

A. Skerra, T G Schmidt, A Skerra, 1993, Protein engineering.

Properties of FV and Fab fragments of the antibody McPC603 expressed in E. coli.

R. Glockshuber, A. Plückthun, A. Skerra, 1990, Behring Institute Mitteilungen.

Analysis and prediction of VH/VL packing in antibodies.

Andrew C. R. Martin, K R Abhinandan, Andrew C R Martin, 2010, Protein engineering, design & selection : PEDS.