K. Ruan

发表

Relationship of the Topological Distances and Activities between mPGES-1 and COX-2 versus COX-1: Implications of the Different Post-Translational Endoplasmic Reticulum Organizations of COX-1 and COX-2.

Hironari Akasaka, S. So, K. Ruan, 2015, Biochemistry.

Aspirin and salicylate bind to immunoglobulin heavy chain binding protein (BiP) and inhibit its ATPase activity in human fibroblasts

Kenneth K. Wu, M. Saunders, Wu-guo Deng, 2001, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

A strategy using NMR peptide structures of thromboxane A2 receptor as templates to construct ligand-recognition pocket of prostacyclin receptor

K. Ruan, Cheng-Huai Ruan, Jaixin Wu, 2005, BMC Biochemistry.

The Protective Effects of Up-Regulating Prostacyclin Biosynthesis on Neuron Survival in Hippocampus

C. Haile, Hironari Akasaka, K. Ruan, 2020, Journal of Neuroimmune Pharmacology.

Ligand-specific conformation determines agonist activation and antagonist blockade in purified human thromboxane A2 receptor.

K. Ruan, Jiaxin Wu, Vanessa Cervantes, 2009, Biochemistry.

Characterization of the prostaglandin H2 mimic: binding to the purified human thromboxane A2 receptor in solution.

K. Ruan, Cori S. Wijaya, Jiaxin Wu, 2008, Archives of biochemistry and biophysics.

A simple, quick, and high-yield preparation of the human thromboxane A2 receptor in full size for structural studies.

K. Ruan, Jiaxin Wu, Vanessa Cervantes, 2008, Biochemistry.

Enhanced prostacyclin synthesis in endothelial cells by retrovirus-mediated transfer of prostaglandin H synthase cDNA.

L. Wang, K. Wu, K. Ruan, 1993, The Journal of clinical investigation.

Prostacyclin therapy for pulmonary arterial hypertension.

R. Dixon, J. Willerson, K. Ruan, 2010, Texas Heart Institute journal.

Establishing novel prostacyclin-synthesizing cells with therapeutic potential against heart diseases.

S. So, K. Ruan, Cheng-Huai Ruan, 2013, International journal of cardiology.

Endothelial-Like Progenitor Cells Engineered to Produce Prostacyclin Rescue Monocrotaline-Induced Pulmonary Arterial Hypertension and Provide Right Ventricle Benefits

R. Dixon, J. Willerson, P. Vanderslice, 2013, Circulation.

The key residue within the second extracellular loop of human EP3 involved in selectively turning down PGE2- and retaining PGE1-mediated signaling in live cells.

Hironari Akasaka, K. Ruan, Qing-Lan Ling, 2017, Archives of biochemistry and biophysics.

Involvement of non‐conserved residues important for PGE2 binding to the constrained EP3 eLP2 using NMR and site‐directed mutagenesis

A. Chillar, S. So, K. Ruan, 2008, FEBS letters.

Prostacyclin is an autocrine regulator in the contraction of oviductal smooth muscle.

J. Goldsby, K. Ruan, Gangxiong Huang, 2002, Human reproduction.

Examination of prostaglandin H synthase-1 topology in the endoplasmic reticulum membrane.

K. Ruan, R. Kulmacz, Y. Ren, 1997, Advances in experimental medicine and biology.

Highly sensitive fluorimetric enzyme immunoassay for prostaglandin H synthase solubilized from cultured cells.

K. Wu, K. Ruan, R. Kulmacz, 1993, Journal of immunological methods.

Inducible COX-2 dominates over COX-1 in prostacyclin biosynthesis: mechanisms of COX-2 inhibitor risk to heart disease.

S. So, K. Ruan, Cheng-Huai Ruan, 2011, Life sciences.

The N-terminal membrane anchor domain of the membrane-bound prostacyclin synthase involved in the substrate presentation of the coupling reaction with cyclooxygenase.

H. Deng, S. So, K. Ruan, 2005, Archives of biochemistry and biophysics.

An active triple‐catalytic hybrid enzyme engineered by linking cyclo‐oxygenase isoform‐1 to prostacyclin synthase that can constantly biosynthesize prostacyclin, the vascular protector

S. So, K. Ruan, Cori S. Wijaya, 2008, The FEBS journal.

Engineered endothelial progenitor cells that overexpress prostacyclin protect vascular cells

R. Dixon, J. Willerson, Yutao Xi, 2012, Journal of cellular physiology.

Novel mechanism of the vascular protector prostacyclin: regulating microRNA expression.

A. Chillar, S. So, K. Ruan, 2011, Biochemistry.

An agonist sensitive, quick and simple cell-based signaling assay for determination of ligands mimicking prostaglandin E2 or E1 activity through subtype EP1 receptor: Suitable for high throughput screening

A. Chillar, K. Ruan, Parastoo Karimi, 2011, BMC complementary and alternative medicine.

More useful maleimide compounds for the conjugation of Fab' to horseradish peroxidase through thiol groups in the hinge.

E. Ishikawa, M. Imagawa, S. Hashida, 1984, Journal of applied biochemistry.

Primary structure of human thromboxane synthase determined from the cDNA sequence.

L. Wang, K. Wu, K. Ruan, 1992, The Journal of biological chemistry.

Advance in understanding the biosynthesis of prostacyclin and thromboxane A2 in the endoplasmic reticulum membrane via the cyclooxygenase pathway.

K. Ruan, 2004, Mini reviews in medicinal chemistry.

Identification of the residues in the helix F/G loop important to catalytic function of membrane-bound prostacyclin synthase.

H. Deng, S. So, K. Ruan, 2003, Biochemistry.

How can we target oxidative stress in the treatment of neurodegenerative diseases?

Hironari Akasaka, K. Ruan, 2016, Future medicinal chemistry.

Acute changes in colonic PGE2 levels as a biomarker of efficacy after treatment of the Pirc (F344/NTac-Apc am1137) rat with celecoxib

Li Li, Taijun Yin, Song Gao, 2019, Inflammation Research.

A Small Scale Preparation of Affinity-Purified Rabbit Fab'-Horseradish Peroxidase Conjugate for Enzyme Immunoassay

E. Ishikawa, S. Hashida, K. Ruan, 1984 .

Reversing thromboxane A2 receptor activity from calcium to cAMP signaling by shifting Gαq to Gαs covalently linked to the receptor

A. Inoue, K. Ruan, Anna Xu, 2022, Biochemical Engineering Journal.

Solution structure of the second extracellular loop of human thromboxane A2 receptor.

J. Wu, S. So, K. Ruan, 2001, Biochemistry.

Topology of prostaglandin H synthase-1 in the endoplasmic reticulum membrane.

K. Ruan, R. Kulmacz, D. Loose-Mitchell, 1995, Archives of biochemistry and biophysics.

Prunella stica inhibits the proliferation but not the prostaglandin production of Ishikawa cells.

K. Ruan, Jaou-Chen Huang, Cheng-Huai Ruan, 2006, Life sciences.

A Small Scale Sandwich Enzyme Immunoassay for Macromolecular Antigens Using B-D-Galactosidase from Escherichia Coli and Horseradish Peroxidase as Labels

Koichiro Tanaka, Y. Niitsu, E. Ishikawa, 1987 .

Small Scale Sandwich Enzyme Immunoassay for Human Chorionic Gonadotropin Using a New Micro-Transferable Plastic Bead as Solid Phase

K. Ruan, D. Ke, 1988, Annals of clinical biochemistry.

High resolution nuclear magnetic resonance spectroscopy-guided mutagenesis for characterization of membrane-bound proteins : Experimental designs and applications 1

K. Ruan, 2014 .

High resolution nuclear magnetic resonance spectroscopy‐guided mutagenesis for characterization of membrane‐bound proteins: Experimental designs and applications

K. Ruan, 2004 .

Role of Val509 in Time-dependent Inhibition of Human Prostaglandin H Synthase-2 Cyclooxygenase Activity by Isoform-selective Agents*

Ke-He Ruan, Qiupeng Guo, Lee-Ho Wang, 1996, The Journal of Biological Chemistry.

Identification of the two-phase mechanism of arachidonic acid regulating inflammatory prostaglandin E2 biosynthesis by targeting COX-2 and mPGES-1.

Hironari Akasaka, K. Ruan, 2016, Archives of biochemistry and biophysics.

Prostacyclin-producing human mesenchymal cells target H19 lncRNA to augment endogenous progenitor function in hindlimb ischaemia

R. Dixon, Yuxiao Deng, J. Willerson, 2016, Nature Communications.

Characterization of the molecular mechanisms of the coupling between intracellular loops of prostacyclin receptor with the C-terminal domain of the Galphas protein in human coronary artery smooth muscle cells.

H. Jüppner, M. Bastepe, Lihai Zhang, 2006, Archives of biochemistry and biophysics.

Physical and Functional Antagonism between Tumor Suppressor Protein p53 and Fortilin, an Anti-apoptotic Protein*

H. Doan, Zhihe Liu, Jui-Yoa Chang, 2011, The Journal of Biological Chemistry.

An Improved Method for the Conjugation of FAB' to B-D-Galactosidase from Escherichia coli

E. Ishikawa, M. Imagawa, S. Hashida, 1984 .

Profile of the regions on the alpha-chain of human acetylcholine receptor recognized by autoantibodies in myasthenia gravis.

M. Atassi, T. Ashizawa, K. Jinnai, 1992, Molecular immunology.

A new strategy, SuperEnzyme gene therapy in penile rehabilitation.

O. Westney, Jiuhong Yuan, K. Ruan, 2009, The journal of sexual medicine.

Structural and functional characterization of the first intracellular loop of human thromboxane A2 receptor.

Shui-Ping So, Jiaxin Wu, Gangxiong Huang, 2004, Archives of biochemistry and biophysics.

Fusion of the β2‐adrenergic receptor with either Gαs or βarrestin‐2 produces constitutive signaling by each pathway and induces gain‐of‐function in BEAS‐2B cells

B. McConnell, R. Bond, A. Reyes-Alcaraz, 2022, FASEB bioAdvances.

COX-2-10aa-PGIS gene therapy improves erectile function in rats after cavernous nerve injury.

Yutian Dai, Jiuhong Yuan, K. Ruan, 2013, The journal of sexual medicine.

Determination of Insulin in Human Saliva Using a More Sensitive Sandwich Enzyme Immunoassay

K. Ruan, D. Ni, Zhixiong Xie, 1988 .

From the design to the clinical application of thromboxane modulators.

Bernard Pirotte, Pierre Drion, D. Praticò, 2006, Current pharmaceutical design.

Creating a mouse model resistant to induced ischemic stroke and cardiovascular damage

Hironari Akasaka, S. So, K. Ruan, 2018, Scientific Reports.

New developments on thromboxane and prostacyclin modulators part I: thromboxane modulators.

P. Kolh, A. Casini, B. Lambermont, 2004, Current medicinal chemistry.

Discovery and Characterization of Dual Inhibitors of MDM2 and NFAT1 for Pancreatic Cancer Therapy.

Ruiwen Zhang, Jianwei Zhou, S. Velu, 2018, Cancer research.

Purification and characterization of a cyclooxygenase‐2 and angiogenesis suppressing factor produced by human fibroblasts

D. Gilroy, Ying-hua Zhu, Kenneth K. Wu, 2002, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

Evidence of the residues involved in ligand recognition in the second extracellular loop of the prostacyclin receptor characterized by high resolution 2D NMR techniques.

Shui-Ping So, Jiaxin Wu, Ke-He Ruan, 2003, Archives of biochemistry and biophysics.

Epitope-specific suppression of antibody response in experimental autoimmune myasthenia gravis by a monomethoxypolyethylene glycol conjugate of a myasthenogenic synthetic peptide.

M. Oshima, M. Atassi, T. Ashizawa, 1992, Proceedings of the National Academy of Sciences of the United States of America.

The short-neurotoxin-binding regions on the alpha-chain of human and Torpedo californica acetylcholine receptors.

M. Atassi, B. Stiles, K. Ruan, 1991, The Biochemical journal.

Engineering 'Enzymelink' for screening lead compounds to inhibit mPGES-1 while maintaining prostacyclin synthase activity.

N. Tang, K. Ruan, Diana T Ruan, 2021, Future medicinal chemistry.

Recent advances of cannabidiol studies in medicinal chemistry, pharmacology and therapeutics.

K. Ruan, Cheng-Huai Ruan, Karishma Thakkar, 2021, Future medicinal chemistry.

Solution structure and topology of the N-terminal membrane anchor domain of a microsomal cytochrome P450: prostaglandin I2 synthase.

W. Zheng, S. So, K. Ruan, 2002, The Biochemical journal.

A Highly Sensitive Enzyme Immunoassay of Anti-Insulin Antibodies in Guinea Pig Serum

E. Ishikawa, T. Kohno, K. Ruan, 1986 .

Identification of Residues Important for Ligand Binding of Thromboxane A2 Receptor in the Second Extracellular Loop Using the NMR Experiment-guided Mutagenesis Approach*

Dawei Li, Shui-Ping So, Jiaxin Wu, 2003, The Journal of Biological Chemistry.

Determination of the membrane contact residues and solution structure of the helix F/G loop of prostaglandin I2 synthase.

S. So, K. Ruan, Jiaxin Wu, 2003, Archives of biochemistry and biophysics.

Structural and functional analysis of the C-terminus of Galphaq in complex with the human thromboxane A2 receptor provides evidence of constitutive activity.

A. Chillar, K. Ruan, Jiaxin Wu, 2010, Biochemistry.

A profile of the residues in the second extracellular loop that are critical for ligand recognition of human prostacyclin receptor

F. Ni, S. So, K. Ruan, 2008, The FEBS journal.

NMR structure of the thromboxane A2 receptor ligand recognition pocket.

S. So, K. Ruan, Jiaxin Wu, 2004, European journal of biochemistry.

Acute changes in colonic PGE2 levels as a biomarker of efficacy after treatment of the Pirc (F344/NTac-Apc am1137) rat with celecoxib

Li Li, Taijun Yin, Song Gao, 2019, Inflammation Research.

IDENTIFICATION OF THE TWO-PHASE MECHANISM OF ENDOGENOUS OMEGA-6 FATTY ACID, ARACHIDONIC ACID, REGULATING VASCULAR INFLAMMATION BY TARGETING CYCLOOXYGENASE-2 AND MICROSOMAL PROSTAGLANDIN E2 SYNTHASE-1

Hironari Akasaka, K. Ruan, 2016 .

Characterization of the secondary structure and membrane interaction of the putative membrane anchor domains of prostaglandin I2 synthase and cytochrome P450 2C1.

K. Wu, K. Ruan, Y. Lin, 1998, Archives of biochemistry and biophysics.

Identification of Tumorigenesis from the Specific Coupling of Cyclooxygenase-2 with Microsomal Prostaglandin E2 Synthase-1 in vivo

N. Tang, A. Chillar, S. So, 2014 .

Latest progress in the development of cyclooxygenase-2 pathway inhibitors targeting microsomal prostaglandin E2 synthase-1.

K. Ruan, Renzhong Lu, Krista LaBorde, 2022, Future medicinal chemistry.

Design, synthesis and characterization of lead compounds as anti-inflammatory drugs targeting mPGES-1 via enzymelink screening.

K. Ruan, Renzhong Lu, Hironori Akasaka, 2023, Future Medicinal Chemistry.

Engineering of a novel hybrid enzyme: an anti-inflammatory drug target with triple catalytic activities directly converting arachidonic acid into the inflammatory prostaglandin E2.

S. So, K. Ruan, Vanessa Cervantes, 2009, Protein engineering, design & selection : PEDS.

Engineering of a protein with cyclooxygenase and prostacyclin synthase activities that converts arachidonic acid to prostacyclin.

H. Deng, S. So, K. Ruan, 2006, Biochemistry.

Current Advances of Microsomal Prostaglandin E Synthase-1 as a Target in Inflammation and Cancer

Hironari Akasaka, K. Ruan, Hawwa Mubaraka Abdul-Malik, 2013 .

Solution structure and topology of the N-terminal membrane anchor domain of a microsomal cytochrome P 450 : prostaglandin I 2 synthase

L. Dawei, W. Zheng, K. Ruan, 2022 .

A More Sensitive and Less Time-Consuming Sandwich Enzyme Immunoassay for Insulin in Human Serum with Less Serum Interference

S. Yoshitake, E. Ishikawa, S. Hashida, 1986, Annals of clinical biochemistry.

A micro-scale affinity-purification of Fab'-horseradish peroxidase conjugates and its use for sandwich enzyme immunoassay of insulin in human serum.

S. Yoshitake, E. Ishikawa, S. Hashida, 1985, Clinica chimica acta; international journal of clinical chemistry.

Recent advances and near future of insulin production and therapy.

K. Ruan, B. King, D. Allen, 2019, Future medicinal chemistry.

Identification of Thromboxane A2 Synthase Active Site Residues by Molecular Modeling-guided Site-directed Mutagenesis*

K. Wu, K. Ruan, R. Kulmacz, 1996, The Journal of Biological Chemistry.

A profile of NSAID-targeted arachidonic acid metabolisms in human embryonic stem cells (hESCs): implication of the negative effects of NSAIDs on heart tissue regeneration.

Y. Geng, H. Shelat, A. Chillar, 2011, International journal of cardiology.

Amino-terminal topology of thromboxane synthase in the endoplasmic reticulum.

L. Wang, K. Wu, K. Ruan, 1993, The Journal of biological chemistry.

Solution structure of a common substrate mimetic of cyclooxygenase-downstream synthases bound to an engineered thromboxane A2 synthase using a high-resolution NMR technique.

K. Ruan, Jiaxin Wu, Lee‐Ho Wang, 2005, Archives of biochemistry and biophysics.

Substrate access channel topology in membrane-bound prostacyclin synthase.

H. Deng, S. So, K. Ruan, 2002, The Biochemical journal.

Identification of the Substrate Interaction Site in the N-terminal Membrane Anchor Segment of Thromboxane A2Synthase by Determination of Its Substrate Analog Conformational Changes Using High Resolution NMR Technique*

S. So, K. Ruan, Dawei Li, 2000, The Journal of Biological Chemistry.

Autoimmune recognition profile of the alpha chain of human acetylcholine receptor in myasthenia gravis.

M. Oshima, M. Atassi, T. Ashizawa, 1991, Advances in experimental medicine and biology.

How can we target oxidative stress in the treatment of neurodegenerative diseases?

Hironari Akasaka, K. Ruan, 2016, Future medicinal chemistry.

Large-scale expression, purification, and characterization of an engineered prostacyclin-synthesizing enzyme with therapeutic potential.

S. So, K. Ruan, Vanessa Cervantes, 2008, Archives of biochemistry and biophysics.

A novel single‐chain enzyme complex with chain reaction properties rapidly producing thromboxane A2 and exhibiting powerful anti‐bleeding functions

S. So, K. Ruan, Qing-Lan Ling, 2019, Journal of cellular and molecular medicine.

Elevated prostacyclin biosynthesis in mice impacts memory and anxiety-like behavior

J. Eriksen, Casey Berridge, Hironari Akasaka, 2014, Behavioural Brain Research.

Discovery and Characterization of Dual Inhibitors of MDM2 and NFAT1 for Pancreatic Cancer Therapy.

Ruiwen Zhang, Jianwei Zhou, S. Velu, 2018, Cancer research.

Solution structure of the third extracellular loop of human thromboxane A2 receptor.

K. Ruan, Jiaxin Wu, Shuiping So, 2003, Archives of biochemistry and biophysics.

A strategy using NMR peptide structures of thromboxane A2 receptor as templates to construct ligand-recognition pocket of prostacyclin receptor

K. Ruan, Cheng-Huai Ruan, Jaixin Wu, 2005, BMC Biochemistry.

Identification of the two-phase mechanism of arachidonic acid regulating inflammatory prostaglandin E2 biosynthesis by targeting COX-2 and mPGES-1.

Hironari Akasaka, K. Ruan, 2016, Archives of biochemistry and biophysics.

How can we address the controversies surrounding the use of NSAIDS in neurodegeneration?

K. Ruan, Qing-Lan Ling, Emma Murdoch, 2016, Future medicinal chemistry.

Assembling NMR structures for the intracellular loops of the human thromboxane A2 receptor: implication of the G protein-coupling pocket.

K. Ruan, Jiaxin Wu, Mary S. Feng, 2008, Archives of biochemistry and biophysics.