Virginijus Siksnys

发表

A pipeline for characterization of novel Cas9 orthologs.

Tautvydas Karvelis, Joshua K Young, Virginijus Siksnys, 2019, Methods in enzymology.

Investigating and Modelling the Target Recognition Dynamics of the CRISPR-Cas Surveillance Complex Cascade

Virginijus Siksnys, M. Rutkauskas, Tomas Sinkūnas, 2018 .

A novel mechanism for the scission of double-stranded DNA : BfiI cuts both 3 0 – 5 0 and 5 0 – 3 0 strands by rotating a single active site

Virginijus Siksnys, Stephen E. Halford, S. Halford, 2009 .

The metal-independent type IIs restriction enzyme BfiI is a dimer that binds two DNA sites but has only one catalytic centre.

Virginijus Siksnys, S. Halford, V. Šikšnys, 2003, Journal of molecular biology.

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

Rolf Backofen, Philippe Horvath, Rodolphe Barrangou, 2019, Nature Reviews Microbiology.

CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement

Stéphane Deschamps, Joshua K Young, Virginijus Siksnys, 2019, Scientific Reports.

Type III CRISPR-Cas Immunity: Major Differences Brushed Aside.

Česlovas Venclovas, Gintautas Tamulaitis, Virginijus Siksnys, 2017, Trends in microbiology.

Programmable RNA shredding by the type III-A CRISPR-Cas system of Streptococcus thermophilus.

Ceslovas Venclovas, Philippe Horvath, Gintautas Tamulaitis, 2014, Molecular cell.

Cas3 is a single‐stranded DNA nuclease and ATP‐dependent helicase in the CRISPR/Cas immune system

Philippe Horvath, Rodolphe Barrangou, Virginijus Siksnys, 2011, The EMBO journal.

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes

Tautvydas Karvelis, Virginijus Siksnys, Ralf Seidel, 2014, Proceedings of the National Academy of Sciences.

crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus

Philippe Horvath, Rodolphe Barrangou, Tautvydas Karvelis, 2013, RNA biology.

Site-specific DNA transesterification catalyzed by a restriction enzyme

Virginijus Siksnys, S. Halford, V. Šikšnys, 2007, Proceedings of the National Academy of Sciences.

Targeted gene editing by transfection of in vitro reconstituted Streptococcus thermophilus Cas9 nuclease complex

Tautvydas Karvelis, Virginijus Siksnys, Elena Maksimova, 2015, RNA biology.

Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements

Tautvydas Karvelis, Virginijus Siksnys, Joshua Young, 2015, Genome Biology.

A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems

Česlovas Venclovas, Gintautas Tamulaitis, Virginijus Siksnys, 2017, Science.

Generation of the BfiI restriction endonuclease from the fusion of a DNA recognition domain to a non-specific nuclease from the phospholipase D superfamily.

Virginijus Siksnys, S. Halford, V. Šikšnys, 2004, Journal of molecular biology.

Spatiotemporal Control of Type III-A CRISPR-Cas Immunity: Coupling DNA Degradation with the Target RNA Recognition.

Česlovas Venclovas, Gintautas Tamulaitis, Virginijus Siksnys, 2016, Molecular cell.

Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific nuclease.

Robert Huber, Manfred Roessle, Virginijus Siksnys, 2005, Proceedings of the National Academy of Sciences of the United States of America.

How the BfiI restriction enzyme uses one active site to cut two DNA strands

Virginijus Siksnys, Stephen E. Halford, S. Halford, 2003, Proceedings of the National Academy of Sciences of the United States of America.

Targeted RNA knockdown by crRNA guided Csm in zebrafish

Gintautas Tamulaitis, Virginijus Siksnys, Matthias Bochtler, 2017, bioRxiv.

The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli

Philippe Horvath, Rodolphe Barrangou, Virginijus Siksnys, 2011, Nucleic acids research.

A novel mechanism for the scission of double-stranded DNA: BfiI cuts both 3′–5′ and 5′–3′ strands by rotating a single active site

Virginijus Siksnys, Stephen E. Halford, S. Halford, 2010, Nucleic acids research.

Methods for decoding Cas9 protospacer adjacent motif (PAM) sequences: A brief overview.

Tautvydas Karvelis, Virginijus Siksnys, G. Gasiunas, 2017, Methods.

Catalytic activity control of restriction endonuclease--triplex forming oligonucleotide conjugates.

Virginijus Siksnys, V. Šikšnys, G. Sasnauskas, 2012, Bioconjugate chemistry.