Minchen Wu

发表

Directed modification of the Aspergillus usamii β-mannanase to improve its substrate affinity by in silico design and site-directed mutagenesis

Jianfang Li, C. Tang, Minchen Wu, 2014, Journal of Industrial Microbiology & Biotechnology.

Improving the thermostability of a mesophilic family 10 xylanase, AuXyn10A, from Aspergillus usamii by in silico design

Zhongbiao Tan, Jing Wu, Jianfang Li, 2014, Journal of Industrial Microbiology & Biotechnology.

Determinants for the improved thermostability of a mesophilic family 11 xylanase predicted by computational methods

Yan-jun Yang, Jianfang Li, Minchen Wu, 2014, Biotechnology for Biofuels.

Engineering hyperthermostability into a mesophilic family 11 xylanase from Aspergillus oryzae by in silico design of N‐terminus substitution

Jianfang Li, Minchen Wu, Shujuan Gao, 2013, Biotechnology and bioengineering.

Highly regio- and enantio-selective hydrolysis of two racemic epoxides by GmEH3, a novel epoxide hydrolase from Glycine max.

Minchen Wu, Xiuxiu Zhu, Chuang Li, 2020, International journal of biological macromolecules.

Significant improvement in catalytic activity and enantioselectivity of a Phaseolus vulgaris epoxide hydrolase, PvEH3, towards ortho-cresyl glycidyl ether based on the semi-rational design

Minchen Wu, Chuang Li, Jun Zhao, 2020, Scientific Reports.

Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design

Jianfang Li, Minchen Wu, D. Hu, 2017, AMB Express.

Contribution of Disulfide Bridges to the Thermostability of a Type A Feruloyl Esterase from Aspergillus usamii

Jianfang Li, Minchen Wu, D. Hu, 2015, PloS one.

Engineering the thermostability of a xylanase from Aspergillus oryzae by an enhancement of the interactions between the N-terminus extension and the β-sheet A2 of the enzyme

Jing Wu, C. Tang, Junqing Wang, 2013, Biotechnology Letters.

Improved temperature characteristics of an Aspergillus oryzae GHF11 xylanase, by in silico design and site-directed mutagenesis

Jianfang Li, Minchen Wu, D. Hu, 2016, Biotechnology and Bioprocess Engineering.

[Effect of N-terminal disulfide bridge on thermostability of family 11 xylanases].

C. Tang, Junqing Wang, S. Gao, 2012, Sheng wu gong cheng xue bao = Chinese journal of biotechnology.

Cloning and optimized expression of a neutral endoglucanase gene (ncel5A) from Volvariella volvacea WX32 in Pichia pastoris.

Jianfang Li, C. Tang, Hongling Shi, 2011, Journal of bioscience and bioengineering.

Cloning and sequence analysis of a novel xylanase gene, Auxyn10A, from Aspergillus usamii

C. Tang, Minchen Wu, Junqing Wang, 2011, Biotechnology Letters.

Stereoselective Hydrolysis of Epoxides by reVrEH3, a Novel Vigna radiata Epoxide Hydrolase with High Enantioselectivity or High and Complementary Regioselectivity.

C. Tang, Minchen Wu, D. Hu, 2017, Journal of agricultural and food chemistry.

Cloning and bioinformatics analysis of an endoglucanase gene (Aucel12A) from Aspergillus usamii and its functional expression in Pichia pastoris

Jianfang Li, C. Tang, Hongling Shi, 2012, Journal of Industrial Microbiology & Biotechnology.

Enhanced acetoin production by Bacillus amyloliquefaciens through improved acetoin tolerance

Qiuling Luo, Jing Wu, Minchen Wu, 2014 .

A Unique Mono- and Diacylglycerol Lipase from Penicillium cyclopium: Heterologous Expression, Biochemical Characterization and Molecular Basis for Its Substrate Selectivity

Zhongbiao Tan, Jing Wu, Jianfang Li, 2014, PloS one.

Enhancing the Thermostability of a Cold-Active Lipase from Penicillium cyclopium by In Silico Design of a Disulfide Bridge

Zhongbiao Tan, Jianfang Li, Minchen Wu, 2014, Applied Biochemistry and Biotechnology.

Determination of amino acids and dipeptides is correlated significantly with optimum temperatures of microbial lipases

Jianfang Li, C. Tang, Hong-Ling Shi, 2013, Annals of Microbiology.

Improvement in the thermostability of a type A feruloyl esterase, AuFaeA, from Aspergillus usamii by iterative saturation mutagenesis

Jianfang Li, Minchen Wu, D. Hu, 2015, Applied Microbiology and Biotechnology.

Enhanced Anti-Tumor (Anti-Proliferation) Activity of Recombinant Human Interleukin-29 (IL-29) Mutants Using Site-Directed Mutagenesis Method

Wei Chen, Minchen Wu, Liyun Li, 2015, Applied Biochemistry and Biotechnology.

Near-perfect kinetic resolution of o-methylphenyl glycidyl ether by RpEH, a novel epoxide hydrolase from Rhodotorula paludigena JNU001 with high stereoselectivity

Minchen Wu, D. Hu, Bochun Hu, 2020, Applied Microbiology and Biotechnology.

Fusing a Carbohydrate-Binding Module into the Aspergillus usamii β-Mannanase to Improve Its Thermostability and Cellulose-Binding Capacity by In Silico Design

Jianfang Li, C. Tang, Minchen Wu, 2013, PloS one.

Enantioconvergent hydrolysis of racemic styrene oxide at high concentration by a pair of novel epoxide hydrolases into (R)-phenyl-1,2-ethanediol

L. Ding, Jianfang Li, Minchen Wu, 2017, Biotechnology Letters.

Characterization of a robust glucose 1-dehydrogenase, SyGDH, and its application in NADPH regeneration for the asymmetric reduction of haloketone by a carbonyl reductase in organic solvent/buffer system

Jianfang Li, Minchen Wu, D. Hu, 2020 .

Directed modification of l-LcLDH1, an l-lactate dehydrogenase from Lactobacillus casei, to improve its specific activity and catalytic efficiency towards phenylpyruvic acid.

Jianfang Li, Minchen Wu, Fengjiao Yuan, 2018, Journal of biotechnology.

Synthesis and biological evaluation of novel bis-aromatic amides as novel PTP1B inhibitors.

F. Nan, Jia Li, Bainian Feng, 2014, Bioorganic & medicinal chemistry letters.

Identification of binding peptides of the ADAM15 disintegrin domain using phage display

Lei Feng, J. Lei, Jian-Chang Jin, 2009, Journal of Biosciences.

Improvement in the catalytic performance of a phenylpyruvate reductase from Lactobacillus plantarum by site-directed and saturation mutagenesis based on the computer-aided design

D. Zhang, Minchen Wu, D. Hu, 2021, 3 Biotech.

Remarkable improvement in the regiocomplementarity of a Glycine max epoxide hydrolase by reshaping its substrate-binding pocket for the enantioconvergent preparation of (R)-hexane-1,2-diol

Minchen Wu, Xiuxiu Zhu, You-Yi Liu, 2021, Molecular Catalysis.

Structure-guided improvement in the enantioselectivity of an Aspergillus usamii epoxide hydrolase for the gram-scale kinetic resolution of ortho-trifluoromethyl styrene oxide.

D. Zhang, Minchen Wu, D. Hu, 2021, Enzyme and microbial technology.

Manipulating the regioselectivity of a Solanum lycopersicum epoxide hydrolase for the enantioconvergent synthesis of enantiopure alkane- and alkene-1,2-diols

D. Zhang, Minchen Wu, D. Hu, 2020 .

Substantially improving the enantioconvergence of PvEH1, a Phaseolus vulgaris epoxide hydrolase, towards m-chlorostyrene oxide by laboratory evolution

D. Hu, Bochun Hu, Chuang Li, 2019, Microbial Cell Factories.

Manipulating regioselectivity of an epoxide hydrolase for single enzymatic synthesis of (R)-1,2-diols from racemic epoxides.

Minchen Wu, F. Xue, D. Hu, 2020, Chemical communications.

Improvement in the activity and enantioconvergency of PvEH3, an epoxide hydrolase from Phaseolus vulgaris, for p-chlorostyrene oxide by site-saturation mutagenesis

Jianfang Li, Bochun Hu, Chuang Li, 2018, Catalysis Communications.

Asymmetric hydrolysis of styrene oxide by PvEH2, a novel Phaseolus vulgaris epoxide hydrolase with extremely high enantioselectivity and regioselectivity

Jianfang Li, Minchen Wu, D. Hu, 2017 .

Directed modification of a novel epoxide hydrolase from Phaseolus vulgaris to improve its enantioconvergence towards styrene epoxides

Jianfang Li, Minchen Wu, D. Hu, 2016 .

Multiple site-directed mutagenesis of a Phaseolus vulgaris epoxide hydrolase to improve its catalytic performance towards p-chlorostyrene oxide based on the computer-aided re-design.

D. Hu, Bochun Hu, Chuang Li, 2019, International Journal of Biological Macromolecules.

Structure-Guided Regulation in the Enantioselectivity of an Epoxide Hydrolase to Produce Enantiomeric Monosubstituted Epoxides and Vicinal Diols via Kinetic Resolution.

Dong Zhang, D. Hu, Bochun Hu, 2022, Organic letters.

Near-perfect kinetic resolution of racemic p-chlorostyrene oxide by SlEH1, a novel epoxide hydrolase from Solanum lycopersicum with extremely high enantioselectivity.

Minchen Wu, D. Hu, Bochun Hu, 2019, International journal of biological macromolecules.

Expression of a novel epoxide hydrolase of Aspergillus usamii E001 in Escherichia coli and its performance in resolution of racemic styrene oxide

Tao Yu, C. Tang, Minchen Wu, 2015, Journal of Industrial Microbiology & Biotechnology.

Improving the activity and enantioselectivity of PvEH1, a Phaseolus vulgaris epoxide hydrolase, for o-methylphenyl glycidyl ether by multiple site-directed mutagenesis on the basis of rational design

Minchen Wu, D. Hu, Chuang Li, 2019, Molecular Catalysis.

Engineering a family 27 carbohydrate-binding module into an Aspergillus usamii β-mannanase to perfect its enzymatic properties.

Jianfang Li, Minchen Wu, Chunjuan Wang, 2017, Journal of bioscience and bioengineering.

High-level heterologous expression of an alkaline lipase gene from Penicillium cyclopium PG37 in Pichia pastoris

Zhongbiao Tan, Jianfang Li, C. Tang, 2011 .

Nearly perfect kinetic resolution of racemic o-nitrostyrene oxide by AuEH2, a microsomal epoxide hydrolase from Aspergillus usamii, with high enantio- and regio-selectivity.

D. Zhang, Minchen Wu, D. Hu, 2020, International journal of biological macromolecules.

Enhanced thermostability of a mesophilic xylanase by N-terminal replacement designed by molecular dynamics simulation.

Jianfang Li, C. Tang, Minchen Wu, 2013, Journal of the science of food and agriculture.

Greatly enhancing the enantioselectivity of PvEH2, a Phaseolus vulgaris epoxide hydrolase, towards racemic 1,2-epoxyhexane via replacing its partial cap-loop.

Minchen Wu, D. Hu, Bochun Hu, 2020, International journal of biological macromolecules.

Correction: A Unique Mono- and Diacylglycerol Lipase from Penicillium cyclopium: Heterologous Expression, Biochemical Characterization and Molecular Basis for Its Substrate Selectivity

Zhongbiao Tan, Jing Wu, Jianfang Li, 2014, PLoS ONE.

Reduction of m-chlorophenacyl chloride coupled with regeneration of NADPH by recombinant Escherichia coli cells co-expressing both carbonyl reductase and glucose 1-dehydrogenase

Yujie Cai, Tao Yu, Jianfang Li, 2015, Annals of Microbiology.

Cloning, expression of a feruloyl esterase from Aspergillus usamii E001 and its applicability in generating ferulic acid from wheat bran

C. Tang, Minchen Wu, Junqing Wang, 2013, Journal of Industrial Microbiology & Biotechnology.

Cloning and functional expression of an acidophilic β-mannanase gene (Anman5A) from Aspergillus niger LW-1 in Pichia pastoris.

Jianfang Li, C. Tang, Minchen Wu, 2012, Journal of agricultural and food chemistry.

Cloning and bioinformatics analysis of a novel acidophilic β-mannanase gene, Auman5A, from Aspergillus usamii YL-01-78

Jiangfan Li, C. Tang, Hongling Shi, 2011 .

Substituting Both the N-Terminal and “Cord” Regions of a Xylanase from Aspergillus oryzae to Improve Its Temperature Characteristics

Jianfang Li, Xueqing Li, Chuang Li, 2018, Applied Biochemistry and Biotechnology.

Expression and Characterization of Hyperthermotolerant Xylanases, SyXyn11P and SyXyn11E, in Pichia pastoris and Escherichia coli

Jianfang Li, Minchen Wu, Peng Zhang, 2014, Applied Biochemistry and Biotechnology.