John Löfblom

发表

Combining phage and staphylococcal surface display for generation of ErbB3-specific Affibody molecules.

Fredrik Y Frejd, John Löfblom, Martin Nilsson, 2011, Protein engineering, design & selection : PEDS.

Targeting HER3 using mono- and bispecific antibodies or alternative scaffolds

Fredrik Y Frejd, S. Ståhl, J. Löfblom, 2016, mAbs.

Affibody Molecules Intended for Receptor-Mediated Transcytosis via the Transferrin Receptor

S. Ståhl, J. Löfblom, H. Lindberg, 2023, Pharmaceuticals.

An Affibody Molecule Is Actively Transported into the Cerebrospinal Fluid via Binding to the Transferrin Receptor

H. Tegel, S. Ståhl, J. Löfblom, 2020, International journal of molecular sciences.

Comparative Evaluation of Two DARPin Variants: Effect of Affinity, Size, and Label on Tumor Targeting Properties.

A. Schulga, J. Löfblom, V. Tolmachev, 2019, Molecular pharmaceutics.

Identification of proteins that specifically recognize and bind protofibrillar aggregates of amyloid-β

T. Härd, E. Wahlberg, M. Sandgren, 2017, Scientific Reports.

Bacterial display in combinatorial protein engineering

John Löfblom, 2011, Biotechnology journal.

Staphylococcal surface display in combinatorial protein engineering and epitope mapping of antibodies.

M. Uhlén, Johan Rockberg, S. Ståhl, 2010, Recent patents on biotechnology.

Dissecting the Structural Organization of Multiprotein Amyloid Aggregates Using a Bottom-Up Approach

H. Zetterberg, J. Löfblom, Himanshu Chaudhary, 2020, ACS chemical neuroscience.

Preclinical Evaluation of [68Ga]Ga-DFO-ZEGFR:2377: A Promising Affibody-Based Probe for Noninvasive PET Imaging of EGFR Expression in Tumors

J. Löfblom, V. Tolmachev, A. Orlova, 2018, Cells.

Influence of composition of cysteine-containing peptide-based chelators on biodistribution of 99mTc-labeled anti-EGFR affibody molecules

J. Löfblom, V. Tolmachev, A. Orlova, 2018, Amino Acids.

Cyclic versus Noncyclic Chelating Scaffold for 89Zr-Labeled ZEGFR:2377 Affibody Bioconjugates Targeting Epidermal Growth Factor Receptor Overexpression

J. Löfblom, V. Tolmachev, A. Orlova, 2017, Molecular pharmaceutics.

The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule

S. Ståhl, J. Löfblom, V. Tolmachev, 2017, Scientific Reports.

Feasibility of imaging of epidermal growth factor receptor expression with ZEGFR:2377 affibody molecule labeled with 99mTc using a peptide-based cysteine-containing chelator

S. Ståhl, J. Löfblom, V. Tolmachev, 2016, International journal of oncology.

PET imaging of epidermal growth factor receptor expression in tumours using 89Zr-labelled ZEGFR:2377 affibody molecules

S. Ståhl, J. Löfblom, V. Tolmachev, 2016, International journal of oncology.

A truncated and dimeric format of an Affibody library on bacteria enables FACS‐mediated isolation of amyloid‐beta aggregation inhibitors with subnanomolar affinity

T. Härd, S. Ståhl, J. Löfblom, 2015, Biotechnology journal.

Affibody molecules: Engineered proteins for therapeutic, diagnostic and biotechnological applications

Fredrik Y Frejd, J. Carlsson, S. Ståhl, 2010, FEBS letters.

Epitope mapping of antibodies using bacterial surface display

M. Uhlén, Johan Rockberg, S. Ståhl, 2008, Nature Methods.

Optimization of HER3 expression imaging using affibody molecules: Influence of chelator for labeling with indium-111

S. Ståhl, J. Löfblom, V. Tolmachev, 2019, Scientific Reports.

Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21(DE3).

D. Drew, M. Strous, Anna Hjelm, 2012, Journal of molecular biology.

Robust Expression of the Human Neonatal Fc Receptor in a Truncated Soluble Form and as a Full-Length Membrane-Bound Protein in Fusion with eGFP

M. Uhlén, J. Löfblom, T. Gräslund, 2013, PloS one.

Comparison of tumor-targeting properties of directly and indirectly radioiodinated designed ankyrin repeat protein (DARPin) G3 variants for molecular imaging of HER2

J. Löfblom, V. Tolmachev, A. Orlova, 2019, International journal of oncology.

Affinity proteins and their generation

S. Ståhl, J. Löfblom, N. Kronqvist, 2013 .

Non-immunoglobulin based protein scaffolds.

Fredrik Y Frejd, S. Ståhl, F. Frejd, 2011, Current opinion in biotechnology.

Parallel Immunizations of Rabbits Using the Same Antigen Yield Antibodies with Similar, but Not Identical, Epitopes

Björn Forsström, M. Uhlén, Johan Rockberg, 2012, PloS one.

Affibody-Mediated Sequestration of Amyloid β Demonstrates Preventive Efficacy in a Transgenic Alzheimer’s Disease Mouse Model

Fredrik Y Frejd, T. Wisniewski, T. Härd, 2019, Front. Aging Neurosci..

Engineering of a bispecific affibody molecule towards HER2 and HER3 by addition of an albumin‐binding domain allows for affinity purification and in vivo half‐life extension

Fredrik Y Frejd, S. Ståhl, J. Löfblom, 2014, Biotechnology journal.

Engineering of Bispecific Affinity Proteins with High Affinity for ERBB2 and Adaptable Binding to Albumin

S. Hober, J. Nilvebrant, Mikael Åstrand, 2014, PloS one.

Affibody Molecules in Biotechnological and Medical Applications.

Fredrik Y Frejd, P. Nygren, S. Ståhl, 2017, Trends in biotechnology.

HER3 PET Imaging: 68Ga-Labeled Affibody Molecules Provide Superior HER3 Contrast to 89Zr-Labeled Antibody and Antibody-Fragment-Based Tracers

S. Ståhl, J. Löfblom, V. Tolmachev, 2021, Cancers.

Inhibiting HER3-Mediated Tumor Cell Growth with Affibody Molecules Engineered to Low Picomolar Affinity by Position-Directed Error-Prone PCR-Like Diversification

Fredrik Y Frejd, I. Höidén-Guthenberg, S. Ståhl, 2013, PloS one.

Insights from engineering the Affibody-Fc interaction with a computational-experimental method

Daniel F. A. R. Dourado, U. H. Danielson, O. Nordesjö, 2017, Protein engineering, design & selection : PEDS.

Exploring epitopes of antibodies toward the human tryptophanyl-tRNA synthetase.

M. Uhlén, Johan Rockberg, S. Ståhl, 2010, New biotechnology.

Affibody-mediated PET imaging of HER3 expression in malignant tumours

S. Ståhl, J. Löfblom, V. Tolmachev, 2015, Scientific Reports.

Comparative evaluation of 111In-labeled NOTA‑conjugated affibody molecules for visualization of HER3 expression in malignant tumors.

S. Ståhl, J. Löfblom, V. Tolmachev, 2015, Oncology reports.

Simultaneous targeting of two ligand-binding sites on VEGFR2 using biparatopic Affibody molecules results in dramatically improved affinity

Fredrik Y Frejd, E. Gunneriusson, S. Ståhl, 2014, Scientific Reports.

Imaging of HER3-expressing xenografts in mice using a 99mTc(CO)3-HEHEHE-ZHER3:08699 affibody molecule

S. Ståhl, J. Löfblom, V. Tolmachev, 2014, European Journal of Nuclear Medicine and Molecular Imaging.

Evaluation of Staphylococcal Cell Surface Display and Flow Cytometry for Postselectional Characterization of Affinity Proteins in Combinatorial Protein Engineering Applications

H. Wernérus, S. Ståhl, J. Sandberg, 2007, Applied and Environmental Microbiology.

Selection of an optimal cysteine-containing peptide-based chelator for labeling of affibody molecules with (188)Re.

S. Ståhl, J. Löfblom, V. Tolmachev, 2014, European journal of medicinal chemistry.

Development and characterization of small bispecific albumin-binding domains with high affinity for ErbB3

S. Hober, J. Nilvebrant, Mikael Åstrand, 2013, Cellular and Molecular Life Sciences.

Influence of Molecular Design on the Targeting Properties of ABD-Fused Mono- and Bi-Valent Anti-HER3 Affibody Therapeutic Constructs

S. Ståhl, J. Löfblom, V. Tolmachev, 2018, Cells.

Comparative Evaluation of Affibody Molecules for Radionuclide Imaging of in Vivo Expression of Carbonic Anhydrase IX.

J. Löfblom, V. Tolmachev, F. Frejd, 2016, Molecular pharmaceutics.

Staphylococcus carnosus: from starter culture to protein engineering platform

S. Ståhl, J. Löfblom, F. Götz, 2017, Applied Microbiology and Biotechnology.

Radionuclide imaging of VEGFR2 in glioma vasculature using biparatopic affibody conjugate: proof-of-principle in a murine model

L. Claesson‐Welsh, S. Ståhl, J. Löfblom, 2018, Theranostics.

A protease substrate profiling method that links site‐specific proteolysis with antibiotic resistance

P. Samuelson, Lisa Sandersjöö, G. Kostallas, 2014, Biotechnology journal.

Surface display of a single-domain antibody library on Gram-positive bacteria

S. Muyldermans, U. Wernery, S. Ståhl, 2012, Cellular and Molecular Life Sciences.

Optimization of electroporation‐mediated transformation: Staphylococcus carnosus as model organism

M. Uhlén, H. Wernérus, S. Ståhl, 2007, Journal of applied microbiology.

Comparative evaluation of affibody- and antibody fragments-based CAIX imaging probes in mice bearing renal cell carcinoma xenografts

J. Bussink, J. Löfblom, V. Tolmachev, 2019, Scientific Reports.

In vitro Blood-Brain barrier model based on recombinant spider silk protein nanomembranes for evaluation of transcytosis capability of biomolecules.

M. Hedhammar, J. Löfblom, L. Hjelm, 2023, Biochemical and biophysical research communications.

Alternative Scaffolds as Bispecific Antibody Mimetics

J. Löfblom, F. Frejd, John Löfblom, 2011 .

Preclinical evaluation of Affibody molecule for PET imaging of human pancreatic islets derived from stem cells

O. Eriksson, S. Vasylovska, P. Cheung, 2023, EJNMMI research.

Increasing thermal stability and improving biodistribution of VEGFR2-binding affibody molecules by a combination of in silico and directed evolution approaches

J. Löfblom, A. Orlova, Rezan Güler, 2020, Scientific Reports.

VEGFR2-Specific Ligands Based on Affibody Molecules Demonstrate Agonistic Effects when Tetrameric in the Soluble Form or Immobilized via Spider Silk.

M. Hedhammar, J. Löfblom, Naresh Thatikonda, 2019, ACS biomaterials science & engineering.

Novel affinity binders for neutralization of vascular endothelial growth factor (VEGF) signaling

S. Ståhl, Filippa Fleetwood, E. Gordon, 2016, Cellular and Molecular Life Sciences.

An anti-sortilin affibody-peptide fusion inhibits sortilin-mediated progranulin degradation

J. Nilvebrant, H. Lindberg, John Löfblom, 2024, Frontiers in immunology.

A new prodrug form of Affibody molecules (pro-Affibody) is selectively activated by cancer-associated proteases

J. Löfblom, A. Jonsson, Lisa Sandersjöö, 2014, Cellular and Molecular Life Sciences.

Evaluating the Therapeutic Efficacy of Mono- and Bivalent Affibody-Based Fusion Proteins Targeting HER3 in a Pancreatic Cancer Xenograft Model

P. Jonasson, S. Ståhl, J. Löfblom, 2020, Pharmaceutics.

Transferrin Receptor Binding BBB-Shuttle Facilitates Brain Delivery of Anti-Aβ-Affibodies

S. Ståhl, J. Löfblom, H. Lindberg, 2022, Pharmaceutical Research.

Improved contrast of affibody-mediated imaging of HER3 expression in mouse xenograft model through co-injection of a trivalent affibody for in vivo blocking of hepatic uptake

S. Ståhl, J. Löfblom, V. Tolmachev, 2019, Scientific Reports.

Affibody‑mediated imaging of EGFR expression in prostate cancer using radiocobalt‑labeled DOTA‑ZEGFR:2377.

S. Ståhl, J. Löfblom, V. Tolmachev, 2018, Oncology reports.

Evaluation of the Therapeutic Potential of a HER3-Binding Affibody Construct TAM-HER3 in Comparison with a Monoclonal Antibody, Seribantumab.

S. Ståhl, J. Löfblom, V. Tolmachev, 2018, Molecular pharmaceutics.

Evaluation of a radiocobalt-labelled affibody molecule for imaging of human epidermal growth factor receptor 3 expression.

S. Ståhl, J. Löfblom, V. Tolmachev, 2017, International journal of oncology.

In vivo evaluation of a novel format of a bivalent HER3-targeting and albumin-binding therapeutic affibody construct

S. Ståhl, J. Löfblom, V. Tolmachev, 2017, Scientific Reports.

188Re-ZHER2:V2, a Promising Affibody-Based Targeting Agent Against HER2-Expressing Tumors: Preclinical Assessment

M. Lubberink, S. Strand, S. Ståhl, 2014, The Journal of Nuclear Medicine.

Agent Against HER2-Expressing Tumors:

M. Lubberink, S. Strand, J. Löfblom, 2014 .

Benefit of Later-Time-Point PET Imaging of HER3 Expression Using Optimized Radiocobalt-Labeled Affibody Molecules

S. Ståhl, J. Löfblom, V. Tolmachev, 2020, International journal of molecular sciences.

Increase in negative charge of 68Ga/chelator complex reduces unspecific hepatic uptake but does not improve imaging properties of HER3-targeting affibody molecules

S. Ståhl, J. Löfblom, V. Tolmachev, 2019, Scientific Reports.

Molecular Design of HER3-Targeting Affibody Molecules: Influence of Chelator and Presence of HEHEHE-Tag on Biodistribution of 68Ga-Labeled Tracers

S. Ståhl, J. Löfblom, V. Tolmachev, 2019, International journal of molecular sciences.

Staphylococcal display for combinatorial protein engineering of a head‐to‐tail affibody dimer binding the Alzheimer amyloid‐β peptide

T. Härd, A. Johansson, S. Ståhl, 2013, Biotechnology journal.

Abstracts of the 33rd International Austrian Winter Symposium

M. Knopp, J. Karp, M. Schwaiger, 2018, EJNMMI Research.

An engineered affibody molecule with pH-dependent binding to FcRn mediates extended circulatory half-life of a fusion protein

M. Uhlén, L. Abrahmsén, C. Ekblad, 2014, Proceedings of the National Academy of Sciences.

Cellular Effects of HER3-Specific Affibody Molecules

I. Höidén-Guthenberg, S. Ståhl, J. Löfblom, 2012, PloS one.

Targeting Tumor Cells Overexpressing the Human Epidermal Growth Factor Receptor 3 with Potent Drug Conjugates Based on Affibody Molecules

S. Ståhl, J. Löfblom, A. Orlova, 2022, Biomedicines.

Influence of Residualizing Properties of the Radiolabel on Radionuclide Molecular Imaging of HER3 Using Affibody Molecules

S. Ståhl, J. Löfblom, V. Tolmachev, 2020, International journal of molecular sciences.

Noninvasive PET Detection of CD69-Positive Immune Cells Before Signs of Clinical Disease in Inflammatory Arthritis

O. Eriksson, B. Mitran, G. Hulsart-Billström, 2023, The Journal of Nuclear Medicine.