Manfred T Reetz

发表

Solid‐Phase Gene Synthesis for Mutant Library Construction: The Future of Directed Evolution?

Zhoutong Sun, Aitao Li, Manfred T Reetz, 2018, Chembiochem : a European journal of chemical biology.

The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes.

Ge Qu, Zhoutong Sun, Aitao Li, 2020, Angewandte Chemie.

Directed Evolution of Proteins Based on Mutational Scanning.

Manfred T Reetz, Carlos G Acevedo-Rocha, Matteo Ferla, 2018, Methods in molecular biology.

A machine learning approach for reliable prediction of amino acid interactions and its application in the directed evolution of enantioselective enzymes

Frédéric Cadet, Nicolas Fontaine, Iyanar Vetrivel, 2018, Scientific Reports.

Methodology Development in Directed Evolution: Exploring Options when Applying Triple‐Code Saturation Mutagenesis

Beibei Liu, Ge Qu, Zhoutong Sun, 2018, Chembiochem : a European journal of chemical biology.

Quantum mechanical/molecular mechanical study on the enantioselectivity of the enzymatic Baeyer-Villiger reaction of 4-hydroxycyclohexanone.

Walter Thiel, Manfred T Reetz, Iakov Polyak, 2013, The journal of physical chemistry. B.

Super-High-Throughput Screening of Enantioselective Catalysts by Using Capillary Array Electrophoresis.

Detlev Belder, Manfred T Reetz, M. Reetz, 2000, Angewandte Chemie.

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

Manfred T Reetz, M. Reetz, J. D. Carballeira, 2007, Nature Protocols.

Designing new Baeyer-Villiger monooxygenases using restricted CASTing.

Manfred T Reetz, Christopher M. Clouthier, M. Reetz, 2006, The Journal of organic chemistry.

Laboratory evolution of enantiocomplementary Candida antarctica lipase B mutants with broad substrate scope.

Manfred T Reetz, Pankaj Soni, M. Reetz, 2013, Journal of the American Chemical Society.

Laboratory evolution of stereoselective enzymes: a prolific source of catalysts for asymmetric reactions.

Manfred T Reetz, M. Reetz, 2011, Angewandte Chemie.

A screening system for enantioselective enzymes based on differential cell growth.

Manfred T Reetz, M. Reetz, Carsten J Rüggeberg, 2002, Chemical communications.

Protein engineering of stereoselective Baeyer-Villiger monooxygenases.

Manfred T Reetz, M. Reetz, Loreto P. Parra, 2012, Chemistry.

Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications.

Manfred T Reetz, M. Reetz, 2004, Proceedings of the National Academy of Sciences of the United States of America.

Enhancing the efficiency of directed evolution in focused enzyme libraries by the adaptive substituent reordering algorithm.

Manfred T Reetz, Joaquin Sanchis, Herschel Rabitz, 2012, Chemistry.

Expanding the range of substrate acceptance of enzymes: combinatorial active-site saturation test.

Andreas Vogel, Manfred T Reetz, Marco Bocola, 2005, Angewandte Chemie.

Constructing and Analyzing the Fitness Landscape of an Experimental Evolutionary Process

Manfred T Reetz, Joaquin Sanchis, M. Reetz, 2008, Chembiochem : a European journal of chemical biology.

Addressing the Numbers Problem in Directed Evolution

Manfred T Reetz, M. Reetz, Daniel Kahakeaw, 2008, Chembiochem : a European journal of chemical biology.

Assembly of Designed Oligonucleotides as an Efficient Method for Gene Recombination: A New Tool in Directed Evolution

Manfred T Reetz, M. Reetz, Dongxing Zha, 2003, Chembiochem : a European journal of chemical biology.

Manipulating the stereoselectivity of limonene epoxide hydrolase by directed evolution based on iterative saturation mutagenesis.

Manfred T Reetz, M. Reetz, Huabao Zheng, 2010, Journal of the American Chemical Society.

Speeding up directed evolution: Combining the advantages of solid-phase combinatorial gene synthesis with statistically guided reduction of screening effort.

Manfred T Reetz, Carlos G Acevedo-Rocha, M. Reetz, 2015, ACS synthetic biology.

Regio- and stereoselectivity of P450-catalysed hydroxylation of steroids controlled by laboratory evolution

Manfred T Reetz, M. Reetz, S. Kille, 2011, Nature Chemistry.

Directed evolution of stereoselective enzymes based on genetic selection as opposed to screening systems.

Manfred T Reetz, Carlos G Acevedo-Rocha, M. Reetz, 2014, Journal of biotechnology.

Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability.

Andreas Vogel, Manfred T Reetz, M. Reetz, 2006, Angewandte Chemie.

Beating Bias in the Directed Evolution of Proteins: Combining High‐Fidelity on‐Chip Solid‐Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction

Tony Cox, Zhoutong Sun, Aitao Li, 2018, Chembiochem : a European journal of chemical biology.

Directed evolution of hybrid enzymes: Evolving enantioselectivity of an achiral Rh-complex anchored to a protein.

Manfred T Reetz, M. Reetz, J. Peyralans, 2006, Chemical communications.

Knowledge-guided laboratory evolution of protein thermolability.

Manfred T Reetz, Pankaj Soni, M. Reetz, 2009, Biotechnology and bioengineering.

Directed Evolution by Using Iterative Saturation Mutagenesis Based on Multiresidue Sites

Manfred T Reetz, M. Reetz, Loreto P. Parra, 2013, Chembiochem : a European journal of chemical biology.

Tuning a p450 enzyme for methane oxidation.

Manfred T Reetz, M. Reetz, Felipe E. Zilly, 2011, Angewandte Chemie.

The importance of additive and non-additive mutational effects in protein engineering.

Manfred T Reetz, M. Reetz, 2013, Angewandte Chemie.

Directed evolution of enantioselective enzymes: an unconventional approach to asymmetric catalysis in organic chemistry.

Manfred T Reetz, M. Reetz, 2009, The Journal of organic chemistry.

Enhancing the enantioselectivity of an epoxide hydrolase by directed evolution.

Yvonne Genzel, Manfred T Reetz, Marco Bocola, 2004, Organic letters.

A practical high-throughput screening system for enantioselectivity by using FTIR spectroscopy.

Matthias Boese, Manfred T Reetz, M. Boese, 2003, Chemistry.

Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space.

Manfred T Reetz, Marco Bocola, M. Reetz, 2006, Angewandte Chemie.

A genetic selection system for evolving enantioselectivity of enzymes.

Manfred T Reetz, Pankaj Soni, M. Reetz, 2008, Chemical communications.

Single-cell high-throughput screening to identify enantioselective hydrolytic enzymes.

Andreas Vogel, Karl-Erich Jaeger, Susanne Wilhelm, 2008, Angewandte Chemie.

Biocatalysis: Chiral cascades.

Manfred T Reetz, M. Reetz, Jian-bo Wang, 2015, Nature chemistry.

Shedding light on the efficacy of laboratory evolution based on iterative saturation mutagenesis.

Manfred T Reetz, Joaquin Sanchis, M. Reetz, 2009, Molecular bioSystems.

Iterative saturation mutagenesis: a powerful approach to engineer proteins by systematically simulating Darwinian evolution.

Manfred T Reetz, Carlos G Acevedo-Rocha, M. Reetz, 2014, Methods in molecular biology.

Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions.

Manfred T Reetz, M. Reetz, Sheng Wu, 2008, Chemical communications.

Time-Resolved IR-Thermographic Detection and Screening of Enantioselectivity in Catalytic Reactions.

Manfred T Reetz, Arnold Holzwarth, M. Reetz, 1998, Angewandte Chemie.

New methods for the high-throughput screening of enantioselective catalysts and biocatalysts.

Manfred T Reetz, M. Reetz, 2002, Angewandte Chemie.

Enantioselective catalysis and analysis on a chip.

Detlev Belder, Manfred T Reetz, M. Reetz, 2006, Angewandte Chemie.

A Method for High-Throughput Screening of Enantioselective Catalysts.

Manfred T Reetz, M. Reetz, Detlef Stöckigt, 1999, Angewandte Chemie.

Manipulating the Expression Rate and Enantioselectivity of an Epoxide Hydrolase by Using Directed Evolution

Manfred T Reetz, M. Reetz, Huabao Zheng, 2011, Chembiochem : a European journal of chemical biology.

Learning from Directed Evolution: Further Lessons from Theoretical Investigations into Cooperative Mutations in Lipase Enantioselectivity

Walter Thiel, Andreas Vogel, Karl-Erich Jaeger, 2007, Chembiochem : a European journal of chemical biology.

Reshaping an enzyme binding pocket for enhanced and inverted stereoselectivity: use of smallest amino acid alphabets in directed evolution.

Zhoutong Sun, Manfred T Reetz, Richard Lonsdale, 2015, Angewandte Chemie.

Expanding the substrate scope of enzymes: combining mutations obtained by CASTing.

Andreas Vogel, Manfred T Reetz, M. Reetz, 2006, Chemistry.

What are the Limitations of Enzymes in Synthetic Organic Chemistry?

Manfred T Reetz, M. Reetz, 2016, Chemical record.

Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity: rigorous comparison with traditional methods.

Yosephine Gumulya, Manfred T Reetz, Marco Bocola, 2010, Journal of the American Chemical Society.

Creation of an amino acid network of structurally coupled residues in the directed evolution of a thermostable enzyme.

Manfred T Reetz, Joaquin Sanchis, Pankaj Soni, 2009, Angewandte Chemie.

Whole-Cell-Catalyzed Multiple Regio- and Stereoselective Functionalizations in Cascade Reactions Enabled by Directed Evolution.

Zhoutong Sun, Aitao Li, Manfred T Reetz, 2016, Angewandte Chemie.

Reducing codon redundancy and screening effort of combinatorial protein libraries created by saturation mutagenesis.

Manfred T Reetz, Carlos G Acevedo-Rocha, D. Opperman, 2013, ACS synthetic biology.

Assembly of Designed Oligonucleotides: a useful tool in synthetic biology for creating high-quality combinatorial DNA libraries.

Manfred T Reetz, Carlos G Acevedo-Rocha, M. Reetz, 2014, Methods in molecular biology.

Artificial metalloenzymes as catalysts in stereoselective Diels-Alder reactions.

Manfred T Reetz, M. Reetz, 2012, Chemical record.

An overview of high-throughput screening systems for enantioselective enzymatic transformations.

Manfred T Reetz, M. Reetz, 2003, Methods in molecular biology.

Many Pathways in Laboratory Evolution Can Lead to Improved Enzymes: How to Escape from Local Minima

Yosephine Gumulya, Manfred T Reetz, Joaquin Sanchis, 2012, Chembiochem : a European journal of chemical biology.

Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method.

Yosephine Gumulya, Manfred T Reetz, Pankaj Soni, 2010, Chemical communications.

Quantum mechanical/molecular mechanical study on the mechanism of the enzymatic Baeyer-Villiger reaction.

Walter Thiel, Manfred T Reetz, Iakov Polyak, 2012, Journal of the American Chemical Society.

Directed evolution of an enantioselective epoxide hydrolase: uncovering the source of enantioselectivity at each evolutionary stage.

Manfred T Reetz, Marco Bocola, Joaquin Sanchis, 2009, Journal of the American Chemical Society.