Induction of Creatine Phosphokinase in Cultures of Chick Skeletal Myoblasts without Concomitant Cell Fusion

The induction of the enzyme creatine phosphokinase (CPK) in cultures of chick breast muscle myoblasts has been distinguished from the process of fusion of myoblasts resulting in the formation of multinucleated myotubes. Primary cultures of myoblasts grown in the presence of phospholipase C, BUdR or EGTA, all of which prevent cell fusion, contain amounts of CPK similar to the level in untreated cultures. Both the brain and muscle isozymes are present in all cultures. We conclude that the induction of CPK is not dependent upon the formation of multinucleated myotubes.

[1]  B. Paterson,et al.  APPEARANCE OF ACETYLCHOLINE RECEPTOR IN DIFFERENTIATING CULTURES OF EMBRYONIC CHICK BREAST MUSCLE , 1973, The Journal of cell biology.

[2]  J. Trotter,et al.  Inhibition of cellular differentiation by phospholipase C. I. Effects of the enzyme on myogenesis and chondrogenesis in vitro. , 1973 .

[3]  G. Fischbach,et al.  The distribution of acetylcholine sensitivity over uninnervated and innervated muscle fibers grown in cell culture. , 1973, Developmental biology.

[4]  W. Loomis,et al.  Temperature-sensitive variants of an established myoblast line. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[5]  T. Easton,et al.  Muscle differentiation in cell culture. Effects of nucleoside inhibitors and Rous sarcoma virus. , 1972, The Journal of biological chemistry.

[6]  E. Ozawa.  The role of calcium ion in avian myogenesis in vitro. , 1972, The Biological bulletin.

[7]  R. Strohman,et al.  Myosin synthesis in cultures of differentiating chicken embryo skeletal muscle. , 1972, Developmental biology.

[8]  H. Weintraub,et al.  The cell cycle, cell lineages, and cell differentiation. , 1972, Current topics in developmental biology.

[9]  J. Rash,et al.  Development of acetylcholine sensitivity during myogenesis. , 1971, Developmental biology.

[10]  G. Yagil,et al.  Alterations of enzymatic activities during muscle differentiation in vitro. , 1971, Developmental biology.

[11]  H. Holtzer,et al.  INHIBITION OF MYOBLAST FUSION AFTER ONE ROUND OF DNA SYNTHESIS IN 5-BROMODEOXYURIDINE , 1970, The Journal of cell biology.

[12]  H. Eppenberger,et al.  [143] Creatine kinase (chicken skeletal and heart muscle; rabbit brain) , 1970 .

[13]  J. R. Coleman,et al.  Muscle differentiation and macromolecular synthesis , 1968, Journal of cellular physiology.

[14]  W. Rutter,et al.  Regulation of specific protein synthesis in cytodifferentiation , 1968, Journal of cellular physiology.

[15]  B. Strehler,et al.  Kinetics of accumulation of creatine phosphokinase activity in developing embryonic skeletal muscle in vivo and in monolayer culture. , 1963, Experimental cell research.

[16]  H. Holtzer,et al.  AN ANALYSIS OF MYOGENESIS BY THE USE OF FLUORESCENT ANTIMYOSIN , 1957, The Journal of biophysical and biochemical cytology.

[17]  R. Dulbecco,et al.  PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES , 1954, The Journal of experimental medicine.

[18]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.