Different Expression of E-Cadherin by Two Cutaneous γ/δ TcR+ T-Cell Subsets, Vγ5- and Vγ5+ γ/δ TcR+ T cells

Recently we have demonstrated that, besides Vγ5 + γ/δ TcR + T cells (Vγ5 + γ/δ T cells), Vγ5 - γ/δslash; TcR + T cells (Vγ5 + γ/δ T cells) are also present in murine skin. In the present study, to characterize the functional differences between these two different cutaneous γ/δ T cells we examined the expression pattern of E-cadherin and its two integrins. After co-culturing of Ly-5 + epidermal cells and migrating cells from organ-cultured murine skin with cutaneous stromal cells, we could expand Vγ5 + γ/δ T cells and Vγ5 - γ/δ T cells, respectively. Flow cytometry demonstrated that cultured Vγ5 + γ/δ T cells expressed E-cadherin, but Vγ5 - γ/δ T cells did not. This difference in E-cadherin expression was also observed in freshly isolated Vγ 5+ and Vγ5 - γ/δ T cells. On the other hand, both Vγ5 + and Vγ5 - γ/δ T cells expressed the a chain of the vitronectin receptor, but did not express the α4 integrin. Of these two cutaneous &/δ T cells, only Vγ5 + γ/δ T cells adhered to murine keratinocyte cell line, PAM 212 cells. Unexpectedly, however, the adhesion of E-cadherin-expressing Vγ5 + γ/δ T cells to PAM 212 cells was not inhibited by anti-E-cadherin antibody, which effectively abrogated the adhesion of Langerhans cells to PAM 212 cells. These distinct phenotypic and functional characteristics in the subsets of cutaneous γ/δ T cells may suggest that they reside in different locations in the skin to play different functional roles in skin immunophysiology.