The characterization of structural variations within a crystal field.

The technique of correlation averaging is refined by the use of multivariate statistical analysis and classification. The refined method can deal with the presence of structural variations within a crystal field. A low-dose image of a crotoxin crystal embedded in ice is used to demonstrate that crystallographic structural parameters characterizing the different areas of such a crystal with varying structure can be extracted rigorously and reproducibly.

[1]  E K Fram,et al.  Reconstruction of imperfectly ordered zinc-induced tubulin sheets using cross-correlation and real space averaging. , 1981, Ultramicroscopy.

[2]  W. O. Saxton,et al.  The correlation averaging of a regularly arranged bacterial cell envelope protein , 1982, Journal of microscopy.

[3]  J Frank,et al.  Multivariate statistical analysis of ribosome electron micrographs. L and R lateral views of the 40 S subunit from HeLa cells. , 1982, Journal of molecular biology.

[4]  R. Henderson,et al.  Three-dimensional structure determination by electron microscopy of two-dimensional crystals. , 1982, Progress in biophysics and molecular biology.

[5]  J Frank,et al.  Reconstitution of molecule images analysed by correspondence analysis: A tool for structural interpretation , 1986, Journal of Microscopy.

[6]  R. Henderson,et al.  Molecular structure determination by electron microscopy of unstained crystalline specimens. , 1975, Journal of molecular biology.

[7]  J Frank,et al.  The structure of the stalk surface layer of a brine pond microorganism: correlation averaging applied to a double layered lattice structure , 1985, Journal of microscopy.

[8]  J. Frank,et al.  Structure of the Channels in the Outer Mitochondrial Membrane: Electron Microscopic Studies of the Periodic Arrays Induced by Phospholipase a(2) Treatment of the Neurospora membrane. , 1986, Biophysical journal.

[9]  Marin van Heel,et al.  Multivariate statistical classification of noisy images (randomly oriented biological macromolecules) , 1984 .

[10]  J Frank,et al.  Classification of images of biomolecular assemblies: a study of ribosomes and ribosomal subunits of Escherichia coli , 1988, Journal of microscopy.

[11]  J. Lepault,et al.  Structure of purple membrane from halobacterium halobium: recording, measurement and evaluation of electron micrographs at 3.5 Å resolution , 1986 .

[12]  J Frank,et al.  Reconstruction of glutamine synthetase using computer averaging. , 1978, Ultramicroscopy.

[13]  Joachim Frank,et al.  Use of multivariate statistics in analysing the images of biological macromolecules , 1981 .

[14]  W. Chiu,et al.  Electron imaging of crotoxin complex thin crystal at 3.5 A. , 1984, Journal of molecular biology.

[15]  W. O. Saxton,et al.  Three-dimensional reconstruction of imperfect two-dimensional crystals. , 1984, Ultramicroscopy.

[16]  W. Chiu,et al.  Low dose electron microscopy of the crotoxin complex thin crystal. , 1983, Journal of molecular biology.

[17]  J Frank,et al.  Motif detection in quantum noise-limited electron micrographs by cross-correlation. , 1977, Ultramicroscopy.

[18]  W. Baumeister,et al.  High resolution shadowing of freeze-dried bacterial photosynthetic membranes: multivariate statistical analysis and surface relief reconstruction , 1985 .