论文信息 - Direct measurement of the adhesive strength of biofilms in pipes by micromanipulation

Direct measurement of the adhesive strength of biofilms in pipes by micromanipulation

A novel micromanipulation technique has been developed to measure directly the adhesive strength of biofilms formed in pipe flows. A T-shaped probe was specially designed to pull Pseudomonas fluorescens biofilms away from the inner surface of a pipe to which they were attached. The adhesive strength between biofilms and the substratum was defined as the work required to remove the biofilms per unit area from the substratum. Results showed that the adhesive strength increased with the fluid velocity in which the biofilms were grown, with a typical value of 0.05∼0.2 J/m2. © Rapid Science Ltd. 1998

[1] M. V. van Loosdrecht,et al. Adhesion and biofilm development on suspended carriers in airlift reactors: hydrodynamic conditions versus surface characteristics. , 1997, Biotechnology and bioengineering.

[2] H. Schlichting. Boundary Layer Theory , 1955 .

[3] J. Costerton,et al. The involvement of cell-to-cell signals in the development of a bacterial biofilm. , 1998, Science.

[4] M. Fletcher,et al. Effects of substratum wettability and molecular topography on the initial adhesion of bacteria to chemically defined substrata a b , 1997 .

[5] S. Kjelleberg,et al. Mechanisms of Adhesion , 1984 .

[6] Michael A. Ferenczi,et al. A micromanipulation technique with a theoretical cell model for determining mechanical properties of single mammalian cells , 1992 .

[7] Hideki Harada,et al. A novel concept for evaluation of biofilm adhesion strength by applying tensile force and shear force , 1996 .