Simple proteomic checks for detecting noncoding RNA

Proper validation can accelerate sequence‐based discovery of proteins and protein‐coding genes. Databases currently contain a backlog of experimentally unverified gene models and tentative assignments of observed transcripts to coding or noncoding RNA. We present and apply a general principle, founded on base composition and the genetic code and validated here by bulk 2‐D gels, that can improve the reliability of such classifications and of the algorithms or pipelines that lead to them.

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