Separation of bacteria with imprinted polymeric films.

Separation of compounds out of complex mixtures is a key issue that has been solved for small molecules by chromatography. However, general methods for the separation of large bio-particles, such as cells, are still challenging. We demonstrate integration of imprinted polymeric films (IPF) into a microfluidic chip, which preferentially capture cells matching an imprint template, and separate strains of cyanobacteria with 80-90% efficiency, despite a minimal difference in morphology and fluorescence, demonstrating its general nature. It is currently thought that the imprinting process, conducted while the polymer cures, transfers chemical information of the cell's external structure to the substrate. Capture specificity and separation can be further enhanced by orienting the imprints parallel to the flow vector and tuning the pH to a lower range.

[1]  Richard N Zare,et al.  Microfluidic purification and analysis of hematopoietic stem cells from bone marrow. , 2011, Lab on a chip.

[2]  James Noble,et al.  The rational development of molecularly imprinted polymer-based sensors for protein detection. , 2011, Chemical Society reviews.

[3]  M. Möder,et al.  Synthesis of a molecularly imprinted polymer and its application for microextraction by packed sorbent for the determination of fluoroquinolone related compounds in water. , 2011, Analytica chimica acta.

[4]  Eric W. Hall,et al.  Particle sorting using a porous membrane in a microfluidic device. , 2011, Lab on a chip.

[5]  K. Haupt,et al.  Molecularly imprinted polymers: synthetic receptors in bioanalysis , 2010, Analytical and bioanalytical chemistry.

[6]  Peter A Lieberzeit,et al.  Imprinted sol-gel materials for monitoring degradation products in automotive oils by shear transverse wave. , 2010, Analytica chimica acta.

[7]  R. Schirhagl,et al.  Antibodies and their replicae in microfluidic sensor systems-labelfree quality assessment in food chemistry and medicine , 2010 .

[8]  Peter A Lieberzeit,et al.  Comparing biomimetic and biological receptors for insulin sensing. , 2010, Chemical communications.

[9]  S. Piletsky,et al.  Catalytic molecularly imprinted polymer membranes: development of the biomimetic sensor for phenols detection. , 2010, Analytica chimica acta.

[10]  Peter A Lieberzeit,et al.  Detection of viruses with molecularly imprinted polymers integrated on a microfluidic biochip using contact-less dielectric microsensors. , 2009, Lab on a chip.

[11]  Nicholas A Peppas,et al.  Mimicking Biological Delivery Through Feedback-Controlled Drug Release Systems Based on Molecular Imprinting. , 2009, AIChE journal. American Institute of Chemical Engineers.

[12]  Franz L Dickert,et al.  Sensing picornaviruses using molecular imprinting techniques on a quartz crystal microbalance. , 2009, Analytical chemistry.

[13]  W. Nelson,et al.  Germ Warfare in a Microbial Mat Community: CRISPRs Provide Insights into the Co-Evolution of Host and Viral Genomes , 2009, PloS one.

[14]  Nicholas A. Peppas,et al.  Molecularly imprinted polymers with specific recognition for macromolecules and proteins , 2008 .

[15]  B. Mizaikoff,et al.  Correlated theoretical, spectroscopic and X-ray crystallographic studies of a non-covalent molecularly imprinted polymerisation system. , 2007, The Analyst.

[16]  B. Mizaikoff,et al.  Investigating the mechanisms of 17β-estradiol imprinting by computational prediction and spectroscopic analysis , 2007, Analytical and bioanalytical chemistry.

[17]  Shuichi Takayama,et al.  Leakage-free bonding of porous membranes into layered microfluidic array systems. , 2007, Analytical chemistry.

[18]  J. Sturm,et al.  Deterministic hydrodynamics: Taking blood apart , 2006, Proceedings of the National Academy of Sciences.

[19]  U. Kolb,et al.  Soluble single-molecule nanogels of controlled structure as a matrix for efficient artificial enzymes. , 2006, Angewandte Chemie.

[20]  K. Markides,et al.  A hybrid poly(dimethylsiloxane) microsystem for on-chip whole blood filtration optimized for steroid screening , 2006, Biomedical microdevices.

[21]  R. J. Umpleby,et al.  Characterization of the imprint effect and the influence of imprinting conditions on affinity, capacity, and heterogeneity in molecularly imprinted polymers using the Freundlich isotherm-affinity distribution analysis. , 2004, Analytical chemistry.

[22]  Sergey A. Piletsky,et al.  Electrochemical Sensors Based on Molecularly Imprinted Polymers , 2002 .

[23]  E. Hoiczyk,et al.  Cyanobacterial Cell Walls: News from an Unusual Prokaryotic Envelope , 2000, Journal of bacteriology.

[24]  F. Dickert,et al.  Artificial receptor layers for detecting chemical and biological threats , 2010 .

[25]  C. M. Barbosa,et al.  POLÍMEROS COM CAPACIDADE DE RECONHECIMENTO MOLECULAR NO CONTROLO DA LIBERTAÇÃO DE FÁRMACOS. PARTE 1: SÍNTESE E CARACTERIZAÇÃO , 2009 .

[26]  F. Dickert,et al.  Synthetic receptors as sensor coatings for molecules and living cells. , 2001, The Analyst.

[27]  A. Klibanov,et al.  Production of abiotic receptors by molecular imprinting of proteins. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[28]  B. Sellergren,et al.  Molecular imprinting of amino acid derivatives in macroporous polymers , 1985 .

[29]  A. Faessler.  X‐Ray Spectroscopic Investigation of Chemical Bonding in Solids , 1972 .