Structure of the porcine LH- and FSH-releasing hormone. I. The proposed amino acid sequence.

Summary The complete amino acid sequence of porcine LH- and FSH- releasing hormone has been provisionally determined by the use on a micro-scale of the combined Edman-dansyl procedure coupled with the selective tritiation method for C-terminal analysis. These procedures were used directly on the digestion products of LH-RH with chymotrypsin and thermolysin, without separation of the fragments. Additional data were provided by high resolution mass spectral fragmentation of LH-RH. On the basis of these results, we propose the following decapeptide sequence for LH-RH: (pyro)Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.

[1]  A. Schally,et al.  Isolation and properties of the FSH and LH-releasing hormone. , 1971, Biochemical and biophysical research communications.

[2]  H. Matsubara,et al.  High recovery of tryptophan from acid hydrolysates of proteins. , 1969, Biochemical and biophysical research communications.

[3]  C. Gros,et al.  Study of the dansylation reaction of amino acids, peptides and proteins. , 2005, European journal of biochemistry.

[4]  B. Hartley.  Strategy and tactics in protein chemistry. , 1970, The Biochemical journal.

[5]  W. White,et al.  Evidence for peptide nature of LH and FSH-releasing hormones. , 1971, Biochemical and biophysical research communications.

[6]  H. Matsuo,et al.  A novel method for the determination of C-terminal amino acid in polypeptides by selective tritium labelling. , 1966, Biochemical and biophysical research communications.

[7]  S Sawano,et al.  Hypothalamic neurohormones regulating anterior pituitary function. , 1968, Recent progress in hormone research.