HUMAN MAMMARY CELL CULTURES

Reduction mammoplasty tissue was used to obtain short-term cultures of human epithelial cell populations. Digestion of tissue with collagenase and hyaluronidase resulted in cell clusters (organoids) resembling ductal and alveolar structures; these could be separated by filtration from the stromal components. Epithelial outgrowth from these organoids was greatly enhanced by the addition of conditioned medium from other human epithelial and myoepithelial cell lines. Additionally, the mammary epithelial growth was stimulated by insulin, hydrocortisone, epidermal growth factor, and steroid hormones. With this enriched nutritional environment, active cell division could be maintained for 1 to 3 months and cells could be serially subcultured 1 to 4 times.

[1]  E. Gaffney,et al.  Origin, concentration and structural features of human mammary gland cells cultured from breast secretions , 1976, Cell and Tissue Research.

[2]  P. Furmanski,et al.  Growth of normal and malignant human mammary epithelial cells in culture. , 1979, Journal of the National Cancer Institute.

[3]  H. Smith.  In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. , 1979, Journal of the National Cancer Institute.

[4]  E. L. Springer,et al.  Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. , 1979, Cancer research.

[5]  M. Lippman,et al.  Growth of a human breast cancer cell line in serum-free hormone-supplemented medium. , 1978, Cancer research.

[6]  S. Nandi,et al.  Primary culture of rat mammary epithelial cells. II. Cytotoxic effect and metabolism of 7,12-dimethylbenz[a]anthracene and N-nitroso-N-methylurea. , 1978, Journal of the National Cancer Institute.

[7]  R. Armstrong,et al.  Cocultivation of human primary breast carcinomas and embryonic mesenchyme resulting in growth and maintenance of tumor cells. , 1978, Cancer research.

[8]  J. Taylor‐Papadimitriou,et al.  Growth requirements of human mammary epithelial cells in culture , 1977, International journal of cancer.

[9]  H. Smith,et al.  Cytologic evaluation of breast fluid in the detection of breast disease. , 1977, Journal of the National Cancer Institute.

[10]  P. Rudland,et al.  Comparison of the effects of hormones on DNA synthesis in cell cultures of nonneoplastic and neoplastic mammary epithelium from rats. , 1977, Cancer research.

[11]  E. L. Springer,et al.  Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs578T) and the diploid myoepithelial (Hs578Bst) cell lines. , 1977, Journal of the National Cancer Institute.

[12]  J. Taylor‐Papadimitriou,et al.  Results on a pilot study of cultures of human lacteal secretions and benign and malignant breast tumors. , 1977, Clinical oncology.

[13]  R. Ceriani,et al.  Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[14]  J. Taylor‐Papadimitriou,et al.  Response to epidermal growth factors of cultured human mammary epithelial cells from benign tumours , 1976, Nature.

[15]  R. Burke,et al.  Cultures of normal human mammary cells. , 1976, Cell differentiation.

[16]  E. L. Springer,et al.  Epithelial cell cultures from normal and cancerous human tissues. , 1976, Journal of the National Cancer Institute.

[17]  W. Nelson-Rees,et al.  The biology of human cells in tissue culture. I. Characterization of cells derived from osteogenic sarcomas , 1976, International journal of cancer.

[18]  E. Lasfargues.  New Approaches to the Cultivation of Human Breast Carcinomas , 1975 .

[19]  J. Whitescarver.  PROBLEMS OF IN VITRO CULTURE OF HUMAN MAMMARY TUMOR CELLS , 1974 .

[20]  S. Nandi,et al.  Influence of seeding density on multicellular organization and nuclear events in cultures of normal and neoplastic mouse mammary epithelium. , 1974, Journal of the National Cancer Institute.

[21]  G. Buehring.  Culture of human mammary epithelial cells: keeping abreast with a new method. , 1972, Journal of the National Cancer Institute.