Comparative study of dot enzyme immunoassay (Typhidot-M) and Widal test in the diagnosis of typhoid fever

We compared the sensitivity and specificity of Typhidot-M and Widal test with blood culture (gold standard) for diagnosing typhoid fever in 105 children aged 1–15 years admitted with clinical suspicion of typhoid fever. Of the 105 cases, blood culture was positive for S.typhi in 41 (39%) children, Widal test was positive in 48 (45.7%) and Typhidot-M was positive in 78 (74.3%) cases. Sensitivity and specificity of Typhidot-M was 92.6% and 37.5% while sensitivity and specificity of Widal test was 34.1% and 42.8%, respectively. In children with fever of less than 7 days duration, Typhidot-M was positive in 97%, compared to 24.2% by Widal test. Typhidot-M is a simple and sensitive test for early diagnosis of typhoid fever in children.

[1]  J. Wain,et al.  Value of a Single-Tube Widal Test in Diagnosis of Typhoid Fever in Vietnam , 1999, Journal of Clinical Microbiology.

[2]  Hal B. Jenson,et al.  Nelson Textbook of Pediatrics , 1965 .

[3]  C. A. Hunter.  Text Book of Microbiology , 1947 .

[4]  L. Olopoenia,et al.  Widal agglutination test − 100 years later: still plagued by controversy , 2000, Postgraduate medical journal.

[5]  N. T. Chinh,et al.  Evaluation of Rapid Diagnostic Tests for Typhoid Fever , 2004, Journal of Clinical Microbiology.

[6]  A. Talati,et al.  Multidrug-Resistant Typhoid Fever , 2000, Tropical doctor.

[7]  M. Jesudason,et al.  Prospective evaluation of a rapid diagnostic test Typhidot for typhoid fever. , 2006, The Indian journal of medical research.

[8]  V. Gopalakrishnan,et al.  Typhoid fever in Kuala Lumpur and a comparative evaluation of two commercial diagnostic kits for the detection of antibodies to Salmonella typhi. , 2002, Singapore medical journal.

[9]  Z. Bhutta,et al.  Rapid serologic diagnosis of pediatric typhoid fever in an endemic area: a prospective comparative evaluation of two dot-enzyme immunoassays and the Widal test. , 1999, The American journal of tropical medicine and hygiene.

[10]  P. Ndumbe,et al.  The increase in occurrence of typhoid fever in Cameroon: overdiagnosis due to misuse of the Widal test? , 2002, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[11]  Shalini,et al.  Multidrug — resistant typhoid fever , 2007, Indian journal of pediatrics.

[12]  M. Jesudason,et al.  Typhidot test to detect IgG & IgM antibodies in typhoid fever. , 2002, The Indian journal of medical research.

[13]  Jacqueline L Deen,et al.  Evaluation of new-generation serologic tests for the diagnosis of typhoid fever: data from a community-based surveillance in Calcutta, India. , 2006, Diagnostic microbiology and infectious disease.

[14]  M. Levine,et al.  Vaccination against typhoid fever: present status. , 1994, Bulletin of the World Health Organization.