Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces

The motion of DNA chain under electric field when it is in transient contact with the solid surfaces in aqueous solution was studied by single molecule fluorescence microscopy at the total internal reflection geometry (TIRFM). In situ observation discovered that single λ-DNA chains driven by electric field made transient contact with the solid surface and made hitting–sliding–leaving-like motion along the surface. By varying the surface chemistry, from the negative-charged silanol group-rich surface to positive-charged amino group-rich surface, as well as hydrophobic surfaces, the dependence of DNA mobility on the surface–DNA interaction was studied. The results show that a dependence of the mobility of DNA on the surface polarity with respect to DNA itself. The study on different surfaces rich of silanol, amide, amino, and methyl groups show a sequence of DNA mobility of silanol > amide > amino. The mobility of DNA on methyl terminated surface was found to be similar to that on amino surface. © 2009 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 47: 2541–2546, 2009

[1]  P. Renaud,et al.  Transport phenomena in nanofluidics , 2008 .

[2]  E. Yeung,et al.  Single DNA molecules as probes for interrogating silica surfaces after various chemical treatments. , 2007, Analytica chimica acta.

[3]  Jiang Zhao,et al.  Guided alignment and positioning of single DNA molecules by a structured contact line on a block copolymer surface. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[4]  E. Yeung,et al.  Motion of single DNA molecules at a liquid-solid interface as revealed by variable-angle evanescent-field microscopy. , 2005, The journal of physical chemistry. B.

[5]  S. Granick,et al.  Polymer lateral diffusion at the solid-liquid interface. , 2004, Journal of the American Chemical Society.

[6]  M. Malmsten.  Biopolymers at Interfaces, Second Edition , 2003 .

[7]  S. Granick,et al.  Perspective: Kinetic and mechanical properties of adsorbed polymer layers , 2002, The European physical journal. E, Soft matter.

[8]  Chia-Fu Chou,et al.  Electrodeless dielectrophoresis of single- and double-stranded DNA. , 2002, Biophysical journal.

[9]  Dilip Gersappe,et al.  Modeling the dynamics of DNA electrophoresis on a flat surface , 2002, Electrophoresis.

[10]  E. Gratton,et al.  Surface Diffusion of Poly(ethylene glycol) , 2002 .

[11]  P. D. Patel,et al.  Electrophoresis of DNA Adsorbed to a Cationic Supported Bilayer , 2001 .

[12]  E. Yeung,et al.  Real-time dynamics of single-DNA molecules undergoing adsorption and desorption at liquid-solid interfaces. , 2001, Analytical chemistry.

[13]  K. Shin,et al.  DNA electrophoresis on a flat surface. , 2000, Physical review letters.

[14]  C. Desruisseaux,et al.  Theory of DNA electrophoresis: A look at some current challenges , 2000, Electrophoresis.

[15]  B. Maier,et al.  DNA on fluid membranes : A model polymer in two dimensions , 2000 .

[16]  M. Akashi,et al.  A Novel Fabrication of Ultrathin Poly(vinylamine) Films with a Molecularly Smooth Surface , 1999 .

[17]  Joachim O. Rädler,et al.  CONFORMATION AND SELF-DIFFUSION OF SINGLE DNA MOLECULES CONFINED TO TWO DIMENSIONS , 1999 .

[18]  Hua Zhang,et al.  Force titration of amino group-terminated self-assembled monolayers using chemical force microscopy , 1998 .

[19]  S Povey,et al.  Dynamic molecular combing: stretching the whole human genome for high-resolution studies. , 1997, Science.

[20]  H. Yamaoka,et al.  EXACT EVALUATION OF CHARACTERISTIC PROTONATION OF POLY(VINYLAMINE) IN AQUEOUS SOLUTION , 1996 .

[21]  A Bensimon,et al.  Alignment and sensitive detection of DNA by a moving interface. , 1994, Science.

[22]  S. Granick,et al.  Formation and Characterization of a Highly Ordered and Well- Anchored Alkylsilane Monolayer on Mica by Self -Assembly , 1991 .

[23]  R. Wu,et al.  Nucleotide sequence analysis of DNA. II. Complete nucleotide sequence of the cohesive ends of bacteriophage lambda DNA. , 1971, Journal of molecular biology.

[24]  A. Katchalsky,et al.  SECTION II: Polybase properties of polyvinylamine , 1957 .