Anticancer Activity of MUC-30 in Breast Cancer Cells

Introduction. Currently, a number of semi-synthetic of natural plant compounds have been developed to improve its effectiveness for treatment of cancers. In the present study, we investigated the anti-proliferative activity and apoptosis induction of MUC-30 in human breast cancer cells (MCF-7). MUC-30, a semi- synthetic analog of lignan, isolated from Phyllanthus taxodiifolius Beille (Krai Hang Nak). Methods. MCF-7 cells were treated with various concentrations of MUC-30 (0.01-10 µM) for 24-72 h and cell viability was evaluated by MTT assay. The anti-proliferative effect of the compound to MCF-7 cells via apoptosis pathway was determined by using DAPI assay, flow cytometry analysis, and the protein expression of PARP-1. Results. MUC-30 potently inhibited the proliferation of MCF-7 cells in a concentration- and time-related manners. Its IC50 (concentration that inhibits 50% of cell growth) was approximately 0.23 ± 0.07 µM at 72 h. The compound induced morphological changes of nucleus, caused an accumulation of apoptotic cell in subG0 phase, and induced the expression of cleaved PARP-1. Conclusion. MUC-30 is a promising potent anti-proliferative agent for MCF-7 cells by inducing apoptosis. The result of this study is important for further development of this compound as a new therapeutic agent for cancer treatment. J Physiol Biomed Sci. 2012; 25(1): 19-22

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