A molecular epidemiological study of rabies epizootics in kudu (Tragelaphus strepsiceros) in Namibia.

BACKGROUND A panel of 37 rabies virus isolates were collected and studied, originating mainly from the northern and central regions of Namibia, between 1980 and 2003. RESULTS These virus isolates demonstrated a high degree of genetic similarity with respect to a 400 bp region of the nucleoprotein gene, with the virus isolates originating from kudu antelope (n = 10) sharing 97.2-100% similarity with jackal isolates, and 97-100% similarity with those isolated from domestic dogs. Phylogenetic analysis suggested that these viruses were all of the canid rabies biotype of southern Africa. The viruses from kudu were closely associated with jackal isolates (n = 6), bat-eared fox isolates (n = 2) and domestic dog isolates (n = 2) at the genetic level and identical at the amino acid level, irrespective of the year of isolation. CONCLUSION These data suggest that jackal and kudu may form part of the same epidemiological cycle of rabies in Namibian wildlife, and might demonstrate the close-relationship between rabies virus strains that circulate within Namibia and those that circulate between Namibia and its neighbouring countries such as Botswana and South Africa.

[1]  C. Rupprecht,et al.  Mongoose rabies in southern Africa: a re-evaluation based on molecular epidemiology. , 2005, Virus research.

[2]  N. Johnson,et al.  Molecular epidemiology of rabies in Botswana: a comparison between antibody typing and nucleotide sequence phylogeny. , 2004, Veterinary microbiology.

[3]  Edward C Holmes,et al.  Phylogenetic analysis reveals a low rate of homologous recombination in negative-sense RNA viruses. , 2003, The Journal of general virology.

[4]  L. Nel,et al.  Molecular epidemiology of canid rabies in Zimbabwe and South Africa. , 2003, Virus research.

[5]  N. Johnson,et al.  Phylogenetic comparison of the genus Lyssavirus using distal coding sequences of the glycoprotein and nucleoprotein genes , 2002, Archives of Virology.

[6]  T. Carpenter,et al.  Temporal patterns of domestic and wildlife rabies in central Namibia stock-ranching area, 1986-1996. , 2000, Preventive veterinary medicine.

[7]  L. McElhinney,et al.  Heminested PCR assay for detection of six genotypes of rabies and rabies-related viruses , 1997, Journal of clinical microbiology.

[8]  K. Strimmer,et al.  Quartet Puzzling: A Quartet Maximum-Likelihood Method for Reconstructing Tree Topologies , 1996 .

[9]  Roderic D. M. Page,et al.  TreeView: an application to display phylogenetic trees on personal computers , 1996, Comput. Appl. Biosci..

[10]  N. Tordo,et al.  Genetic polymorphism in the rabies virus nucleoprotein gene. , 1995, Virology.

[11]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[12]  J. Nel.  The bat-eared fox: a prime candidate for rabies vector? , 1993, The Onderstepoort journal of veterinary research.

[13]  R. Swanepoel,et al.  Rabies in southern Africa. , 1993, The Onderstepoort journal of veterinary research.

[14]  O. Hübschle.  Rabies in the kudu antelope (Tragelaphus strepsiceros). , 1988, Reviews of infectious diseases.

[15]  B. Barnard,et al.  Non-bite transmission of rabies in kudu (Tragelaphus strepsiceros). , 1982, The Onderstepoort journal of veterinary research.

[16]  R. Hassel.  Incidence of rabies in kudu in South West Africa Namibia , 1982 .

[17]  B. Barnard.  The role played by wildlife in the epizootiology of rabies in South Africa and South-West Africa. , 1979, The Onderstepoort journal of veterinary research.

[18]  R. P. Bell.  A Review of the Present Position , 1920, The Hospital.

[19]  A. King,et al.  Europe as a source of rabies for the rest of the world. , 2004 .

[20]  L. Nel,et al.  Molecular epidemiology of rabies virus in South Africa: evidence for two distinct virus groups. , 1995, The Journal of general virology.

[21]  H. Berry.  Surveillance and control of anthrax and rabies in wild herbivores and carnivores in Namibia. , 1993, Revue scientifique et technique.

[22]  G. Thomson,et al.  Canid and viverrid rabies viruses in South Africa. , 1993, The Onderstepoort journal of veterinary research.

[23]  B. Barnard,et al.  Rabies in kudus (Tragelaphus strepsiceros) in South West Africa/Namibia. , 1981, Journal of the South African Veterinary Association.

[24]  L. V. Maltitz.  Rabies in the Northern Districts of South West Africa. , 1950 .

[25]  P. S. Snyman.  The study and control of the vectors of rabies in South Africa , 1940 .