Determination of Arsenobetaine in Manufactured Seafood Products by Liquid Chromatography, Microwave-assisted Oxidation and Hydride Generation Atomic Absorption Spectrometry

A study was carried out to develop and optimize a method for determining arsenobetaine (AB) in seafood products by coupling HPLC, microwave-assisted oxidation and HGAAS. Conditions were established for the extraction and instrumental determination of AB in popular seafood products. The analytical features of the method were as follows: the detection limit was 0.68–27.20 ng g -1 As (fresh mass), the relative standard deviation ranged from 0.4 to 6%, and the recovery was 104±5%. The analysis of DORM-1 (Dogfish Muscle, National Research Council of Canada, Certified Reference Material) provided an AB value of 16.5±0.6 µg g -1 As (dry mass), in agreement with results obtained by other workers using HPLC–ICP-MS. The proposed procedure was used to analyse canned seafood products purchased at local retail market outlets. The contents of total As represented by AB ranged from 5 to 75%. The lowest percentages of AB corresponded, in general, to the bivalve group.

[1]  D. Vélez,et al.  Optimization of the extraction and determination of monomethylarsonic and dimethylarsinic acids in seafood products by coupling liquid chromatography with hydride generation atomic absorption spectrometry , 1996 .

[2]  D. Vélez,et al.  Percentages of Total Arsenic Represented by Arsenobetaine Levels of Manufactured Seafood Products , 1995 .

[3]  M. Gómez,et al.  Evaluation of high-performance liquid chromatography for the separation and determination of arsenic species by on-line high-performance liquid chromatographic-hydride generation-atomic absorption spectrometry. , 1995, Journal of chromatography. B, Biomedical applications.

[4]  C. Cámara,et al.  Determination of toxic and non-toxic arsenic species in urine by microwave assisted mineralization and hydride generation atomic absorption spectrometry , 1995 .

[5]  E. Larsen.  Speciation of dimethylarsinyl-riboside derivatives (arsenosugars) in marine reference materials by HPLC-ICP-MS , 1995 .

[6]  D. Vélez,et al.  Optimization of the extraction, clean-up and determination of arsenobetaine in manufactured seafood products by coupling liquid chromatography with inductively coupled plasma atomic emission spectrometry , 1995 .

[7]  R. Rubio,et al.  Arsenic speciation in marine biological materials by LC-UV-HG-ICP/OES , 1995 .

[8]  F. Petrucci,et al.  On-line speciation of arsenical compounds in fish and mussel extracts by HPLC-ICP-MS , 1994, Environmental science and pollution research international.

[9]  M. Vahter.  What are the chemical forms of arsenic in urine, and what can they tell us about exposure? , 1994, Clinical chemistry.

[10]  X. Le,et al.  Speciation of arsenic compounds by HPLC with hydride generation atomic absorption spectrometry and inductively coupled plasma mass spectrometry detection. , 1994, Talanta.

[11]  C. Cámara,et al.  On-line microwave oxidation for the determination of organoarsenic compounds by high-performance liquid chromatography–hydride generation atomic absorption spectrometry , 1994 .

[12]  L. Ebdon,et al.  Determination of arsenic species in fish by directly coupled high-performance liquid chromatography-inductively coupled plasma mass spectrometry , 1994 .

[13]  A. Howard,et al.  Coupled photooxidation-hydride AAS detector for the HPLC of arsenic compounds , 1993 .

[14]  M. Gómez,et al.  Determination of six arsenic species by high-performance liquid chromatography — hydride generation — atomic absorption spectrometry with on-line thermo-oxidation , 1993 .

[15]  S. Hansen,et al.  Arsenic speciation in seafood samples with emphasis on minor constituents: an investigation using high-performance liquid chromatography with detection by inductively coupled plasma mass spectrometry , 1993 .

[16]  M. Morita,et al.  Characterization of organic arsenic compounds in bivalves , 1992 .

[17]  K. Reimer,et al.  Decomposition of organoarsenic compounds by using a microwave oven and subsequent determination by flow injection‐hydride generation‐atomic absorption spectrometry , 1992 .

[18]  M. L. Cervera,et al.  Determination of arsenic in dry ashed seafood products by hydride generation atomic absorption spectrometry and a critical comparative study with platform furnace Zeeman-effect atomic absorption spectrometry and inductively coupled plasma atomic emission spectrometry , 1992 .

[19]  M. Morita,et al.  Determination of arsenic species in biological and environmental samples (Technical Report) , 1992 .

[20]  D. Kalman,et al.  On-line photo-oxidation for the determination of organoarsenic compounds by atomic-absorption spectrometry with continuous arsine generation. , 1991, Talanta.

[21]  D. Phillips.  Arsenic in aquatic organisms: a review, emphasizing chemical speciation , 1990 .

[22]  M. Morita,et al.  Exchange of comments on identification and quantitation of arsenic species in a dogfish muscle reference material for trace elements , 1989 .

[23]  D. Beauchemin,et al.  Identification and quantitation of arsenic species in a dogfish muscle reference material for trace elements. , 1988, Analytical chemistry.

[24]  T. Kaise,et al.  Determination of inorganic arsenic and organic arsenic compounds in marine organisms by hydride generation/cold trap/gas chromatography— mass spectrometry , 1988 .