Rapid extraction and high-performance liquid chromatography of chlorophylls and carotenoids from marine phytoplankton

Abstract An improved technique for analysis of photosynthetic pigments from phytoplankton is described. High efficiency extraction, based on sonication in methanol and centrifugal filtration, removes the cell debris from the initial pigment extract within 2 min of starting disruption. The pigments are separated by reversed-phase high-performance liquid chromatography, using a linear gradient from 90% acetonitrile to ethyl acetate. Carotenes, xanthophylls, chlorophylls and their degradation products are separated with greater resolution than has been previously reported in a single separation. The elution order is presented for 44 pigments from representatives of the Bacilliarophyceae, Dinophyceae, Prymnesiophyceae, Prasinophyceae, Chlorophyceae, Cryptophyceae, Rhodophyceae and Cynophyceae.

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