v-src inhibits differentiation via an extracellular intermediate(s)

Murine 3T3L1 preadipocytes transformed by avian sarcoma virus were unable to differentiate in response to insulin or dexamethasone plus 1-methyl-3-isobutylxanthine, both potent inducers of differentiation of the nontransformed 3T3L1 parental line. Conditioned medium from transformed cells contained a relatively heat-stable factor(s) which inhibited the differentiation of untransformed parental 3T3L1 cells but did not induce any changes in their morphology. A protease-sensitive mitogen was also detected in the medium. The relationship between the two activities remains to be elucidated.

[1]  T. Graf,et al.  Autocrine growth induced by src-related oncogenes in transformed chicken myeloid cells , 1984, Cell.

[2]  D. Twardzik,et al.  Neuroblastoma cells produce transforming growth factors during exponential growth in a defined hormone-free medium. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[3]  R. Ross,et al.  Production of platelet-derived growth factor-like molecules and reduced expression of platelet-derived growth factor receptors accompany transformation by a wide spectrum of agents. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[4]  L. Cantley,et al.  Evidence that the Rous sarcoma virus transforming gene product phosphorylates phosphatidylinositol and diacylglycerol. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[5]  D. Boettiger,et al.  Effect of src infection on long-term marrow cultures: Increased self-renewal of hemopoietic progenitor cells without leukemia , 1984, Cell.

[6]  S. Kawai.  Transformation of rat cells by fusion-infection with Rous sarcoma virus , 1980, Journal of virology.

[7]  J. Greenberger,et al.  Murine sarcoma viruses block corticosteroid-induced differentiation of bone marrow preadipocytes associated with long-term in vitro hemopoiesis. , 1979, Virology.

[8]  H. Green,et al.  Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells. , 1979, The Journal of biological chemistry.

[9]  O. Rosen,et al.  Development of hormone receptors and hormonal responsiveness in vitro. Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells. , 1978, The Journal of biological chemistry.

[10]  R. Erikson,et al.  Protein kinase activity associated with the avian sarcoma virus src gene product. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[11]  J. Brugge,et al.  Identification of a transformation-specific antigen induced by an avian sarcoma virus , 1977, Nature.

[12]  H. Green,et al.  Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells , 1976, Cell.

[13]  H. Green,et al.  An established preadipose cell line and its differentiation in culture II. Factors affecting the adipose conversion , 1975, Cell.

[14]  H. Green,et al.  Sublines of mouse 3T3 cells that accumulate lipid , 1974 .

[15]  R. Eisenman,et al.  Synthesis of avian RNA tumor virus structural proteins. , 1975, Cold Spring Harbor symposia on quantitative biology.