Calculation and Simulation of Temperature Control in Real-time PCR Instrument

In this paper,we designed a model of the PCR reaction chamber by ourselves and calculated the temperature of the reaction chamber and the sample.On the basis of the temperature calculation,we simulated and analyzed the temperature field's dynamic distribution of the reaction system to find that the simulative result and the calculating result match each other.This offers an academic foundation to develop a self-design real-time PCR instrument.