Technologies supporting analytical cytology: clinical, research and drug discovery applications

The tools and techniques developed for analytical cytology have become invaluable in expanding the development of cancer screening programs and biomarker discovery for personalized medicine. Detecting cellular, molecular, and functional changes of diseased tissue as defined by quantitative analytical methodologies has enhanced the field of medical diagnostics and prognostics. The focus of this review is to outline applications and recent technical advances in flow cytometry, laser scanning cytometry, image cytometry, and quantitative image analysis, as they pertain to clinical, research, and drug discovery applications. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

[1]  Peter Sonderegger,et al.  Automated quantification of synapses by fluorescence microscopy , 2012, Journal of Neuroscience Methods.

[2]  H. Moch,et al.  Flow cytometry as an accurate tool to complement fine needle aspiration cytology in the diagnosis of low grade malignant lymphomas , 2011, Cytopathology : official journal of the British Society for Clinical Cytology.

[3]  C. MacAulay,et al.  Squamous cell carcinoma and precursor lesions: diagnosis and screening in a technical era. , 2011, Periodontology 2000.

[4]  C. R. Leemans,et al.  Prognostic value of DNA ploidy status in patients with oral leukoplakia. , 2011, Oral oncology.

[5]  X. Zhao,et al.  Optimizing antibody panels for efficient and cost‐effective flow cytometric diagnosis of acute leukemia , 2011, Cytometry. Part B, Clinical cytometry.

[6]  S. Sundaram,et al.  Morphology to morphometry in cytological evaluation of thyroid lesions , 2011, Journal of cytology.

[7]  C. Gerharz,et al.  Equivocal cytology in lung cancer diagnosis , 2011, Cancer cytopathology.

[8]  P. Speight,et al.  High incidences of DNA ploidy abnormalities in tongue squamous cell carcinoma of young patients: an international collaborative study , 2011, Histopathology.

[9]  P. Lansdorp,et al.  Analysis of repetitive DNA in chromosomes by flow cytometry , 2011, Nature Methods.

[10]  C. Earle,et al.  Timeliness and quality of diagnostic care for medicare recipients with chronic lymphocytic leukemia , 2011, Cancer.

[11]  S. Lam,et al.  Incremental Value of Pulmonary Function and Sputum DNA Image Cytometry in Lung Cancer Risk Prediction , 2011, Cancer Prevention Research.

[12]  V. Leite,et al.  High prevalence of RAS mutations in RET-negative sporadic medullary thyroid carcinomas. , 2011, The Journal of clinical endocrinology and metabolism.

[13]  A. Mittag,et al.  Introduction A: recent advances in cytometry instrumentation, probes, and methods--review. , 2011, Methods in cell biology.

[14]  Stefan Biesterfeld,et al.  Feulgen staining remains the gold standard for precise DNA image cytometry. , 2011, Anticancer research.

[15]  T Smitha,et al.  Morphometry of the basal cell layer of oral leukoplakia and oral squamous cell carcinoma using computer-aided image analysis , 2011, Journal of oral and maxillofacial pathology : JOMFP.

[16]  L. De Mattos-Arruda,et al.  Circulating tumor cells in metastatic breast cancer: the need for a standardized approach. , 2011, Annals of oncology : official journal of the European Society for Medical Oncology.

[17]  S. Natarajan,et al.  Prediction of lymph node metastases by preoperative nuclear morphometry in oral squamous cell carcinoma: a comparative image analysis study. , 2010, Indian journal of cancer.

[18]  D. Katsaros,et al.  Functional role and prognostic significance of CD157 in ovarian carcinoma. , 2010, Journal of the National Cancer Institute.

[19]  M. Papotti,et al.  Utility of Flow Cytometry Immunophenotyping in Fine-needle Aspirate Cytologic Diagnosis of Non-Hodgkin Lymphoma: A Series of 252 Cases and Review of the Literature , 2010, Applied immunohistochemistry & molecular morphology : AIMM.

[20]  Christopher R Shea,et al.  Benign versus malignant parakeratosis: a nuclear morphometry study , 2010, Modern Pathology.

[21]  Rui Cui,et al.  Detection of circulating tumor cells in breast cancer patients utilizing multiparameter flow cytometry and assessment of the prognosis of patients in different CTCs levels , 2010, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[22]  N. Thomas.  Review Article: High-Content Screening: A Decade of Evolution , 2010, Journal of biomolecular screening.

[23]  G. Marti,et al.  Commentary: Comparison of current flow cytometry methods for monoclonal B cell lymphocytosis detection , 2010, Cytometry. Part B, Clinical cytometry.

[24]  Neil E Caporaso,et al.  B-cell clones as early markers for chronic lymphocytic leukemia. , 2009, The New England journal of medicine.

[25]  Ali Bashashati,et al.  A Survey of Flow Cytometry Data Analysis Methods , 2009, Adv. Bioinformatics.

[26]  H. Tanke,et al.  Rapid flow cytometric method for measuring senescence associated β‐galactosidase activity in human fibroblasts , 2009, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[27]  M. Schiffman,et al.  Screening and prevention methods for cervical cancer. , 2009, JAMA.

[28]  Marc Arbyn,et al.  Comparison of liquid-based cytology with conventional cytology for detection of cervical cancer precursors: a randomized controlled trial. , 2009, JAMA.

[29]  Fuzhou Wang,et al.  DNA Ploidy Cytometry Testing for Cervical Cancer Screening in China (DNACIC Trial): a Prospective Randomized, Controlled Trial , 2009, Clinical Cancer Research.

[30]  F. Preffer,et al.  Advances in complex multiparameter flow cytometry technology: Applications in stem cell research , 2009, Cytometry. Part B, Clinical cytometry.

[31]  G. Hortobagyi,et al.  Circulating tumor cells and [18F]fluorodeoxyglucose positron emission tomography/computed tomography for outcome prediction in metastatic breast cancer. , 2009, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[32]  A. A. Bell,et al.  Toward a multimodal cell analysis of brush biopsies for the early detection of oral cancer , 2009, Cancer.

[33]  J. Mesirov,et al.  Automated high-dimensional flow cytometric data analysis , 2009, Proceedings of the National Academy of Sciences.

[34]  Anju Thomas,et al.  Flow cytometry can diagnose classical hodgkin lymphoma in lymph nodes with high sensitivity and specificity. , 2009, American journal of clinical pathology.

[35]  Jonathan M Irish,et al.  Single-cell profiling identifies aberrant STAT5 activation in myeloid malignancies with specific clinical and biologic correlates. , 2008, Cancer cell.

[36]  T. Shanafelt,et al.  MBL or CLL: which classification best categorizes the clinical course of patients with an absolute lymphocyte count >or= 5 x 10(9) L(-1) but a B-cell lymphocyte count <5 x 10(9) L(-1)? , 2008, Leukemia research.

[37]  C. Clavel,et al.  Contribution of DNA ploidy image cytometry to the management of ASC cervical lesions , 2008, Cancer.

[38]  S. Richards,et al.  Monoclonal B-cell lymphocytosis and chronic lymphocytic leukemia. , 2008, The New England journal of medicine.

[39]  K. McGrath,et al.  Multispectral imaging of hematopoietic cells: where flow meets morphology. , 2008, Journal of immunological methods.

[40]  H. Sugimura,et al.  Human Sgo1 downregulation leads to chromosomal instability in colorectal cancer , 2008, Gut.

[41]  R. Montironi,et al.  Laser capture microdissection in the genomic and proteomic era: targeting the genetic basis of cancer. , 2008, International journal of clinical and experimental pathology.

[42]  Marc Arbyn,et al.  Liquid Compared With Conventional Cervical Cytology: A Systematic Review and Meta-analysis , 2008, Obstetrics and gynecology.

[43]  G Janossy,et al.  Affordable CD4 T‐cell enumeration for resource‐limited regions: A status report for 2008 , 2008, Cytometry. Part B, Clinical cytometry.

[44]  G. Marti,et al.  Overview of monoclonal B‐cell lymphocytosis , 2007, British journal of haematology.

[45]  Daniel M Reinders,et al.  Detection of Lung Cancer by Automated Sputum Cytometry , 2007, Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer.

[46]  M. Harnett,et al.  Laser scanning cytometry: understanding the immune system in situ , 2007, Nature Reviews Immunology.

[47]  Martial Guillaud,et al.  Human papillomavirus‐related cellular changes measured by cytometric analysis of DNA ploidy and chromatin texture , 2007, Cytometry. Part B, Clinical cytometry.

[48]  William E. Ortyn,et al.  Cellular image analysis and imaging by flow cytometry. , 2007, Clinics in laboratory medicine.

[49]  Vladislav V Verkhusha,et al.  New lasers for flow cytometry: filling the gaps , 2007, Nature Methods.

[50]  M. Bangerter,et al.  Fine Needle Aspiration Cytology and Flow Cytometry in the Diagnosis and Subclassification of Non-Hodgkin’s Lymphoma Based on the World Health Organization Classification , 2007, Acta Cytologica.

[51]  S. Thibodeau,et al.  Higher Frequency of Diploidy in Young-Onset Microsatellite-Stable Colorectal Cancer , 2007, Clinical Cancer Research.

[52]  Fern S Miller,et al.  Implementation of the ThinPrep® imaging system in a high‐volume metropolitan laboratory , 2007, Diagnostic cytopathology.

[53]  J. Byrd,et al.  International standardized approach for flow cytometric residual disease monitoring in chronic lymphocytic leukaemia , 2007, Leukemia.

[54]  Richard L Lozano,et al.  Comparison of computer-assisted and manual screening of cervical cytology. , 2007, Gynecologic oncology.

[55]  J. Lillard,et al.  The ImageStream System: a key step to a new era in imaging. , 2007, Folia histochemica et cytobiologica.

[56]  P. Lansdorp,et al.  Flow cytometry and FISH to measure the average length of telomeres (flow FISH) , 2006, Nature Protocols.

[57]  R. White,et al.  Flow cytometry after bromodeoxyuridine labeling to measure S and G2+M phase durations plus doubling times in vitro and in vivo , 2006, Nature Protocols.

[58]  E. Petricoin,et al.  Laser Capture Microdissection , 1996, Science.

[59]  Wei Zhang,et al.  Biomarker analysis on breast ductal lavage cells in women with and without breast cancer , 2006, International journal of cancer.

[60]  Martial Guillaud,et al.  DNA ploidy compared with human papilloma virus testing (Hybrid Capture II) and conventional cervical cytology as a primary screening test for cervical high‐grade lesions and cancer in 1555 patients with biopsy confirmation , 2006, Cancer.

[61]  G. T. Budd,et al.  Circulating Tumor Cells at Each Follow-up Time Point during Therapy of Metastatic Breast Cancer Patients Predict Progression-Free and Overall Survival , 2006, Clinical Cancer Research.

[62]  E S Costa,et al.  A new automated flow cytometry data analysis approach for the diagnostic screening of neoplastic B-cell disorders in peripheral blood samples with absolute lymphocytosis , 2006, Leukemia.

[63]  A. Mittag,et al.  Iterative restaining as a pivotal tool for n‐color immunophenotyping by slide‐based cytometry , 2006, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[64]  Steven W Graves,et al.  Ultrasonic particle‐concentration for sheathless focusing of particles for analysis in a flow cytometer , 2006, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[65]  T. Furuya,et al.  Genomic alterations detected by comparative genomic hybridization in primary lung adenocarcinomas with special reference to the relationship with DNA ploidy. , 2005, Oncology reports.

[66]  Martial Guillaud,et al.  Subvisual chromatin changes in cervical epithelium measured by texture image analysis and correlated with HPV. , 2005, Gynecologic oncology.

[67]  Beverly Packard,et al.  DPSS yellow‐green 561‐nm lasers for improved fluorochrome detection by flow cytometry , 2005, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[68]  Branko Palcic,et al.  Malignancy associated changes in epithelial cells of buccal mucosa: a potential cancer detection test. , 2005, Analytical and quantitative cytology and histology.

[69]  Paolo Ghia,et al.  Diagnostic criteria for monoclonal B‐cell lymphocytosis , 2005, British journal of haematology.

[70]  K. Jöckel,et al.  Predictive value of image cytometry for diagnosis of lung cancer in heavy smokers , 2005, European Respiratory Journal.

[71]  B. Dziura,et al.  Assisted primary screening using the automated ThinPrep Imaging System. , 2005, American journal of clinical pathology.

[72]  Martial Guillaud,et al.  Nuclear morphometry as a biomarker for bronchial intraepithelial neoplasia: Correlation with genetic damage and cancer development , 2005, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[73]  S. Lam,et al.  A Randomized Phase IIb Trial of Pulmicort Turbuhaler (Budesonide) in People with Dysplasia of the Bronchial Epithelium , 2004, Clinical Cancer Research.

[74]  S. Kawauchi,et al.  Two subtypes of mucinous colorectal carcinoma characterized by laser scanning cytometry and comparative genomic hybridization. , 2004, International journal of oncology.

[75]  P. Chattopadhyay,et al.  Seventeen-colour flow cytometry: unravelling the immune system , 2004, Nature Reviews Immunology.

[76]  Jonathan M Irish,et al.  Single Cell Profiling of Potentiated Phospho-Protein Networks in Cancer Cells , 2004, Cell.

[77]  Martial Guillaud,et al.  Exploratory analysis of quantitative histopathology of cervical intraepithelial neoplasia: Objectivity, reproducibility, malignancy‐associated changes, and human papillomavirus , 2004, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[78]  F. Hirsch,et al.  Chemoprevention of lung cancer--from biology to clinical reality. , 2004, Annals of oncology : official journal of the European Society for Medical Oncology.

[79]  W. Telford,et al.  Multiparametric analysis of apoptosis by flow and image cytometry. , 2004, Methods in molecular biology.

[80]  Thomas Würflinger,et al.  Robust automatic coregistration, segmentation, and classification of cell nuclei in multimodal cytopathological microscopic images. , 2004, Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society.

[81]  Peter O. Krutzik,et al.  Intracellular phospho‐protein staining techniques for flow cytometry: Monitoring single cell signaling events , 2003, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[82]  K. Syrjänen,et al.  Applicability of liquid-based cytology to the assessment of DNA content in cervical lesions using static cytometry. , 2003, Analytical and quantitative cytology and histology.

[83]  M. Schilham,et al.  Minimal residual disease prior to stem cell transplant for childhood acute lymphoblastic leukaemia , 2003, British journal of haematology.

[84]  M. Raffeld,et al.  Minimal Residual Disease Detection in Hairy Cell Leukemia , 2003 .

[85]  M. Raffeld,et al.  Minimal residual disease detection in hairy cell leukemia. Comparison of flow cytometric immunophenotyping with clonal analysis using consensus primer polymerase chain reaction for the heavy chain gene. , 2003, American journal of clinical pathology.

[86]  Ralph Weissleder,et al.  Magnetic relaxation switches capable of sensing molecular interactions , 2002, Nature Biotechnology.

[87]  S. Lam,et al.  A randomized phase IIb trial of anethole dithiolethione in smokers with bronchial dysplasia. , 2002, Journal of the National Cancer Institute.

[88]  Calum MacAulay,et al.  Increase of sensitivity of sputum cytology using high-resolution image cytometry: field study results. , 2002, Cytometry.

[89]  Z. Grossman,et al.  HIV preferentially infects HIV-specific CD4+ T cells , 2002, Nature.

[90]  David C Wilbur,et al.  The Primary Screening Clinical Trials of the TriPath AutoPap® System , 2002, Epidemiology.

[91]  Garry P. Nolan,et al.  Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry , 2002, Nature Biotechnology.

[92]  B. Bain,et al.  Revised guideline on immunophenotyping in acute leukaemias and chronic lymphoproliferative disorders. , 2002, Clinical and laboratory haematology.

[93]  Danielle Roman,et al.  Laser technologies in toxicopathology. , 2002, Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie.

[94]  M. Roederer,et al.  Probability binning comparison: a metric for quantitating univariate distribution differences. , 2001, Cytometry.

[95]  M Roederer,et al.  Frequency difference gating: a multivariate method for identifying subsets that differ between samples. , 2001, Cytometry.

[96]  G. Morgan,et al.  Quantitation of minimal disease levels in chronic lymphocytic leukemia using a sensitive flow cytometric assay improves the prediction of outcome and can be used to optimize therapy. , 2001, Blood.

[97]  Rafael Núñez,et al.  DNA measurement and cell cycle analysis by flow cytometry. , 2001, Current issues in molecular biology.

[98]  P. V. van Diest,et al.  Nuclear cytometric changes in breast carcinogenesis , 2001, The Journal of pathology.

[99]  G. Haroske,et al.  Fourth Updated ESACP Consensus Report on Diagnostic DNA Image Cytometry , 2001, Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology.

[100]  A. Böcking,et al.  Cytologic and DNA-Cytometric Early Diagnosis of Oral Cancer , 2001, Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology.

[101]  M. Melamed,et al.  A brief history of flow cytometry and sorting. , 2001, Methods in cell biology.

[102]  L. Mango,et al.  Quality Control of Cervical Cytology in High-Risk Women , 2000, Acta Cytologica.

[103]  Z. Darżynkiewicz,et al.  The Schrödinger's cat quandary in cell biology: integration of live cell functional assays with measurements of fixed cells in analysis of apoptosis. , 1999, Experimental cell research.

[104]  M. Melamed,et al.  Laser-scanning cytometry: A new instrumentation with many applications. , 1999, Experimental cell research.

[105]  M. Melamed,et al.  Analysis of apoptosis by laser scanning cytometry. , 1999, Cytometry.

[106]  E. Weir,et al.  A limited antibody panel can distinguish B-precursor acute lymphoblastic leukemia from normal B precursors with four color flow cytometry: implications for residual disease detection , 1999, Leukemia.

[107]  B. Dutrillaux,et al.  Fluorescence‐based analysis of DNA ploidy and cell proliferation within fine‐needle samplings of breast tumors , 1998, Cancer.

[108]  M. Bobadilla,et al.  Flow cytometry as a valuable tool in toxicology , 1998 .

[109]  A Coldman,et al.  Localization of bronchial intraepithelial neoplastic lesions by fluorescence bronchoscopy. , 1998, Chest.

[110]  W Suter,et al.  Evaluation of a new procedure for the flow cytometric analysis of in vitro, chemically induced micronuclei in V79 cells , 1998, Environmental and molecular mutagenesis.

[111]  Jennings Cd,et al.  Recent Advances in Flow Cytometry: Application to the Diagnosis of Hematologic Malignancy , 1997 .

[112]  F. Behm,et al.  U.S.-Canadian Consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: selection of antibody combinations. , 1997, Cytometry.

[113]  B Palcic,et al.  The use of an automated image cytometer for screening and quantitative assessment of cervical lesions in the British Columbia Cervical Smear Screening Programme , 1997, Cytopathology : official journal of the British Society for Clinical Cytology.

[114]  B Palcic,et al.  Sputum screening by quantitative microscopy: a reexamination of a portion of the National Cancer Institute Cooperative Early Lung Cancer Study. , 1997, Mayo Clinic proceedings.

[115]  Evaluation of the PAPNET system for prescreening triage of cervicovaginal smears. , 1997, Acta cytologica.

[116]  L. Suter,et al.  Three-parameter flow cytometric analysis of rat spermatogenesis. , 1997, Cytometry.

[117]  J. Walloch,et al.  Multiparameter immunophenotypic analysis of fine needle aspiration biopsies and other hematologic specimens by laser scanning cytometry. , 1997, Acta cytologica.

[118]  L. Kamentsky,et al.  Slide-based laser scanning cytometry. , 1997, Acta cytologica.

[119]  L. Liotta,et al.  Laser Capture Microdissection , 1996, Science.

[120]  M. Melamed,et al.  Laser scanning cytometer (LSC) analysis of fraction of labelled mitoses (FLM) , 1996, Cell proliferation.

[121]  G. Birdsong.  Automated screening of cervical cytology specimens. , 1996, Human pathology.

[122]  C. Cohen.  Image cytometric analysis in pathology. , 1996, Human pathology.

[123]  K. Sasaki,et al.  Intracellular localization of cyclin B1 during the cell cycle in glioma cells. , 1996, Cytometry.

[124]  M. Melamed,et al.  Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color. , 1996, Experimental cell research.

[125]  X. M. Sun,et al.  Discrimination of apoptotic thymocytes by forward light scatter. , 1995, Cytometry.

[126]  M. Melamed,et al.  Single-step procedure for labeling DNA strand breaks with fluorescein- or BODIPY-conjugated deoxynucleotides: detection of apoptosis and bromodeoxyuridine incorporation. , 1995, Cytometry.

[127]  L. Mango.  Computer-assisted cervical cancer screening using neural networks. , 1994, Cancer Letters.

[128]  R. Bray.  Chapter 7 Flow Cytometry Crossmatching for Solid Organ Transplantation , 1994 .

[129]  L Mariuzzi,et al.  Cytometric evidence that cervical intraepithelial neoplasia I and II are dysplasias rather than true neoplasias. An image analysis study of factors involved in the progression of cervical lesions. , 1992, Analytical and quantitative cytology and histology.

[130]  P H Bartels,et al.  Karyometric features in nuclei near colonic adenocarcinoma. Statistical analysis. , 1991, Analytical and quantitative cytology and histology.

[131]  M. Pahlplatz,et al.  Cytophotometric analysis of corresponding cytological and histological cervical intraepithelial neoplasia grade III specimens. , 1991, Cytometry.

[132]  L. Kamentsky,et al.  Microscope-based multiparameter laser scanning cytometer yielding data comparable to flow cytometry data. , 1991, Cytometry.

[133]  A. Zetterberg,et al.  Ploidy level and tumor progression in prostatic carcinoma. , 1991, Acta oncologica.

[134]  B. Stenkvist,et al.  Cytological and DNA characteristics of hyperplasia/inflammation and cancer of the prostate. , 1990, European journal of cancer.

[135]  M. Pahlplatz,et al.  Cytophotometric analysis of cervical intraepithelial neoplasia grade III, with and without synchronous invasive squamous cell carcinoma. , 1990, Cytometry.

[136]  P H Bartels,et al.  Karyometric marker features in normal-appearing glands adjacent to human colonic adenocarcinoma. , 1990, Cancer research.

[137]  D. Hedley.  Flow cytometry using paraffin-embedded tissue: five years on. , 1989, Cytometry.

[138]  M. Pahlplatz,et al.  DNA ploidy patterns in cervical intraepithelial neoplasia grade III, with and without synchronous invasive squamous cell carcinoma: Measurements in nuclei isolated from paraffin‐embedded tissue , 1988, Cancer.

[139]  P. Zimmerman,et al.  PLOIDY AS A PROGNOSTIC DETERMINANT IN SURGICALLY TREATED LUNG CANCER , 1987, The Lancet.

[140]  K Okamoto,et al.  Automated cytologic screening system (CYBEST model 4): an integrated image cytometry system. , 1987, Applied optics.

[141]  B. Stenkvist,et al.  Automatic analysis of Papanicolaou smears by digital image processing. , 1987, Gynecologic oncology.

[142]  C. Cornelisse,et al.  DNA ploidy and survival in breast cancer patients. , 1987, Cytometry.

[143]  W. Auffermann,et al.  DNA-cytometric diagnosis of prospective malignancy in borderline lesions of the uterine cervix. , 1986, Acta cytologica.

[144]  D. Longo,et al.  Response to 2'-deoxycoformycin after failure of interferon-alpha in nonsplenectomized patients with hairy cell leukemia. , 1986, Blood.

[145]  K. Foon,et al.  Immunologic classification of leukemia and lymphoma. , 1986, Blood.

[146]  K. Preston.  High-resolution image analysis. , 1986, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[147]  W. Auffermann,et al.  Early detection of precancerous lesions in dysplasias of the lung by rapid DNA image cytometry. , 1985, Analytical and quantitative cytology and histology.

[148]  I. Taylor,et al.  Application of DNA flow cytometry to paraffin-embedded archival material for the study of aneuploidy and its clinical significance. , 1985, Cytometry.

[149]  J. Rothfeld,et al.  Quantitative immunocytochemistry of hypothalamic and pituitary hormones: validation of an automated, computerized image analysis system. , 1985, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[150]  I W Taylor,et al.  Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. , 1983, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[151]  P. Kjellstrand.  Mechanisms of the Feulgen acid hydrolysis , 1980, Journal of microscopy.

[152]  P. Bartels,et al.  Computer-based diagnostic analysis of cells in the urinary sediment. , 1980, The Journal of urology.

[153]  H. Gratzner,et al.  An immunocytochemical approach to cell kinetics automation. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[154]  P. Bartels,et al.  Ultrafast laser scanner microscope. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[155]  Godfried T. Toussaint,et al.  Classification of atypical cells in the automatic cytoscreening for cervical cancer , 1979, Pattern Recognit..

[156]  J. Holmquist,et al.  Computer analysis of cervical cells. Automatic feature extraction and classification. , 1978, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[157]  P. Bartels,et al.  Diagnostic cytologic sample profiles in patients with bladder cancer using TICAS system. , 1978, Acta cytologica.

[158]  B Stenkvist,et al.  A software system to record and analyze digitized cell images. , 1977, Computer programs in biomedicine.

[159]  Y. Imasato,et al.  Fundamental study of automatic cyto-screening for uterine cancer. III. New system of automated apparatus (CYBEST) utilizing the pattern recognition method. , 1977, Acta cytologica.

[160]  J. Holmquist,et al.  Analysis of grey-level histograms as a method of classifying cells from the uterine cervix. , 1976, Computers in biology and medicine.

[161]  B. Stenkvist,et al.  Classification of cells in vaginal smears with television microscopy , 1976, Virchows Archiv. B, Cell pathology.

[162]  J H Tucker.  CERVISCAN: an image analysis system for experiments in automatic cervical smear prescreening. , 1976, Computers and biomedical research, an international journal.

[163]  B Stenkvist,et al.  SCANCANS--an interactive scanning cell analysis system. , 1976, Computer programs in biomedicine.

[164]  R G Sweet,et al.  Fluorescence Activated Cell Sorting , 2020, Definitions.

[165]  J. Taylor,et al.  Computer discrimination between benign and malignant urothelial cells. , 1975, Acta cytologica.

[166]  J. Taylor,et al.  Development and evaluation of automatic nucleus finding routines: thresholding of cervical cytology images. , 1975, Acta cytologica.

[167]  P H Bartels,et al.  Computer discrimination of T and B lymphocytes. , 1975, Acta cytologica.

[168]  S. Newsom,et al.  LOCAL CHEMOTHERAPY FOR PSEUDOMONAS LUNG ABSCESS , 1974 .

[169]  D. K. Green,et al.  THE DESIGN, OPERATION AND EVALUATION OF A HIGH SPEED AUTOMATIC METAPHASE FINDER , 1974, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[170]  P. Bartels,et al.  PERFORMANCE TESTING FOR AUTOMATED PRESCREENING DEVICES IN CERVICAL CYTOLOGY , 1974, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[171]  D. K. Green.  Machine to find metaphase cells. , 1974, Experimental cell research.

[172]  J. Rowiński,et al.  Malignancy associated changes (MAC) in cells of buccal smears detected by means of objective image analysis. , 1974, Acta cytologica.

[173]  J. Taylor,et al.  Cervical cytology: descriptive statistics for nuclei of normal and atypical cell types. , 1973, Acta cytologica.

[174]  J. Taylor,et al.  Computer recognition of cell nuclei from the uterine cervix. , 1973, Acta cytologica.

[175]  P. Bartels,et al.  Evaluation of the Papanicolaou stain for computer assisted cellular pattern recognition. , 1973, Acta Cytologica.

[176]  M. Ingram,et al.  Automatic analysis of blood cells. , 1970, Scientific American.

[177]  W. Bonner,et al.  Cell Sorting: Automated Separation of Mammalian Cells as a Function of Intracellular Fluorescence , 1969, Science.

[178]  P. F. Mullaney,et al.  Cell Microfluorometry: A Method for Rapid Fluorescence Measurement , 1969, Science.

[179]  S. Rosenberg,et al.  Automatic Identification and Measurement of Cells by Computer , 1969, Science.

[180]  W. Dittrich,et al.  Notizen: Impulsfluorometrie bei Einzelzellen in Suspensionen , 1969 .

[181]  Nieburgs He,et al.  Recent progress in the interpretation of malignancy associated changes (MAC). , 1968 .

[182]  McMaster Gw.  A measurement of the pattern of normal and malignant cervical cells. , 1968 .

[183]  C. P. Heanley,et al.  Cesar: cervical smear analyser and reader , 1967, Journal of clinical pathology.

[184]  M L Mendelsohn,et al.  THE ANALYSIS OF CELL IMAGES * , 1966, Annals of the New York Academy of Sciences.

[185]  L. Kamentsky,et al.  Spectrophotometer: New Instrument for Ultrarapid Cell Analysis , 1965, Science.

[186]  E. G. Diacumakos,et al.  EXFOLIATED CELL STUDIES AND THE CYTOANALYZER * , 1962, Annals of the New York Academy of Sciences.

[187]  W J HORVATH,et al.  A study of the quantitative characteristics of exfoliated cells from the female genital tract. I. Measurement methods and results , 1961, Cancer.

[188]  W. Tolles,et al.  A study of the quantitative characteristics of exfoliated cells from the female genital tract. II. Suitability of quantitative cytological measurements for automatic prescreening , 1961, Cancer.

[189]  R C MELLORS,et al.  A micro-fluorometric scanner for the differential detection of cells; application of exfoliative cytology. , 1951, Science.

[190]  O. C. Gruner,et al.  A study of the changes met with in the leucocytes in certain cases of malignant disease , 1915 .