Dioxin detection based on immunoassay using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD).

An enzyme-linked immunosorbent assay (ELISA) using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD) is presented. This method is based on a competitive reaction between OCDD and OCDD-HRP (horseradish peroxidase) conjugate against the antibody, whereby OCDD-HRP is detected colorimetrically at 450 nm. The detection limit of OCDD was 0.78 pg mL(-1). Optimizing the reaction conditions of the assay, cross reactivities of some dioxins against the antibody are discussed.

[1]  P. Vercellini,et al.  Seveso Women's Health Study: a study of the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on reproductive health. , 2000, Chemosphere.

[2]  T. Matsuo,et al.  Polychlorinated dibenzo-p-dioxins and dibenzofurans in sediment, soil, fish, shellfish and crab samples from Tokyo Bay area, Japan. , 2000, Chemosphere.

[3]  X. Zhou,et al.  High sensitivity microgravimetric biosensor for qualitative and quantitative diagnostic detection of polychlorinated dibenzo-p-dioxins. , 2001, The Analyst.

[4]  W Farland,et al.  Dioxins: WHO's tolerable daily intake (TDI) revisited. , 2000, Chemosphere.

[5]  Michael G. Ikonomou,et al.  Differentiation of polychlorinated dibenzo‐p‐dioxin and dibenzofuran sources in coastal British Columbia, Canada , 1999 .

[6]  S Marklund,et al.  The influence of level and chlorine source on the formation of mono- to octa-chlorinated dibenzo-p-dioxins, dibenzofurans and coplanar polychlorinated biphenyls during combustion of an artificial municipal waste. , 2001, Chemosphere.

[7]  R O Harrison,et al.  An immunoassay for TEQ screening of dioxin/furan samples: current status of assay and applications development. , 1997, Chemosphere.

[8]  B D Hammock,et al.  Development of a highly sensitive enzyme-linked immunosorbent assay based on polyclonal antibodies for the detection of polychlorinated dibenzo-p-dioxins. , 1998, Analytical chemistry.

[9]  D. Schrenk.  Impact of dioxin-type induction of drug-metabolizing enzymes on the metabolism of endo- and xenobiotics. , 1998, Biochemical pharmacology.

[10]  K. Kuroda,et al.  Peroxidase catalyzed co-polymerization of pentachlorophenol and a potential humic precursor , 2000 .