Nitrogen efficiency of wheat: Genotypic and environmental variation and prospects for improvement

Abstract Winter wheat ( Triticum aestivum L.) was grown for 4 years in multi-factorial field trials at Rothamsted, southern England. Thirty nine elite commercial cultivars (primarily short-straw) were grown including those released in the UK over a 25-year period, a selection of continental varieties, and three older, tall varieties. Varieties spanned the quality spectrum from ‘bread’ to ‘feed’. The crops were given ammonium nitrate at five rates in the range 0–350 kg-N/ha as a 3-way split. The aim was to quantify the genotypic variation in total nitrogen uptake by grain and straw (total-Nup), and in nitrogen utilization efficiency for grain yield (grain yield per unit of N taken up) (grain-NutE). Depending on treatment, grain yield ranged from 2.1 to 11.8 t/ha (85% DM), grain %N from 1.1% to 2.8% (in DM), total-Nup from 31 to 264 kg-N/ha, and grain-NutE from 27 to 77 kg-DM/kg-N. There were significant varietal differences in total N-uptake and grain-NutE both between ‘tall’ and ‘short’ varieties and within ‘short’ varieties. The best short varieties took up 31–38 kg/ha more N than the worst, and grain-NutE was 24–42% better, depending on N-rate. Up to 77% of the variation in grain-NutE was accounted for by yield. All interactions between the factors ‘Variety’, ‘Year’, and ‘N-rate’ were highly significant, but only ‘Year × N-rate’ made an important contribution to the variation. There was a near-functional inverse relationship between grain-NutE and grain %N; high-quality wheat (high grain %N) can be expected to have a low grain-NutE. The four key variables determining N-efficiency in a wheat crop – grain yield, grain %N, total N-uptake and nitrogen harvest index (NHI) – are ultimately constrained by the law of conservation of matter. Improving grain-NutE for fixed total-Nup and NHI can only be achieved at the expense of grain %N. To improve grain-NutE and maintain grain %N requires a simultaneous increase in NHI and grain starch yield which may be difficult to achieve in practice. The law of conservation of matter ultimately sets a limit on the physiological and agronomic processes that determine crop N requirements. A high yield of high-quality grain (high grain %N) requires a high input and uptake of nitrogen.