Optimization of biogas production from wheat straw stillage in UASB reactor

In the present study, thermophilic anaerobic digestion of wheat straw stillage was investigated. Methane potential of stillage was determined in batch experiments at two different substrate concentrations. Results showed that higher methane yields of 324 ml/g-(volatile solids) VSadded were obtained at stillage concentrations of 12.8 g-VS/L than at 25.6 g-VS/l. Continuous anaerobic digestion of stillage was performed in an up-flow anaerobic sludge blanket (UASB) reactor at 55 °C with 2 days hydraulic retention time. Results showed that both substrate concentration and organic loading rate (OLR) influenced process performance and methane yields. Maximum methane yield of 155 ml CH4/g-COD was obtained at stillage mixtures with water of 25% (v/v) in the feed and at an OLR of 17.1 g-COD/(l.d). Soluble chemical oxygen demand (SCOD) removal at this OLR was 76% (w/w). Increase in OLR to 41.2 g-COD/(l.d) and/or stillage concentration in the feed to 33-50% (v/v) resulted in low methane yields or complete process failure. The results showed that thermophilic anaerobic digestion of wheat straw stillage alone for methane production is feasible in UASB reactor at an OLR of 17.1 g-COD/(l.d) and at substrate concentration of 25% in the feed. The produced methane could improve the process energy and economics of a bioethanol plant and also enable to utilize the stillage in a sustainable manner.

[1]  A. Guwy,et al.  Defining the biomethane potential (BMP) of solid organic wastes and energy crops: a proposed protocol for batch assays. , 2009, Water science and technology : a journal of the International Association on Water Pollution Research.

[2]  Yue-Qin Tang,et al.  Anaerobic treatment performance and microbial population of thermophilic upflow anaerobic filter reactor treating awamori distillery wastewater. , 2007, Journal of bioscience and bioengineering.

[3]  Jan Larsen,et al.  The IBUS Process – Lignocellulosic Bioethanol Close to a Commercial Reality , 2008 .

[4]  A. Wilkie,et al.  Stillage characterization and anaerobic treatment of ethanol stillage from conventional and cellulosic feedstocks , 2000 .

[5]  Anton Friedl,et al.  Analysis and decrease of the energy demand of bioethanol-production by process integration , 2007 .

[6]  B. Ahring,et al.  Effects of lipids on thermophilic anaerobic digestion and reduction of lipid inhibition upon addition of bentonite , 1990, Applied Microbiology and Biotechnology.

[7]  J. Lester,et al.  The Behaviour of Chlorinated Organics during Activated Sludge Treatment and Anaerobic Digestion , 1988 .

[8]  Małgorzata Krzywonos,et al.  Utilization and biodegradation of starch stillage (distillery wastewater) , 2009 .

[9]  A. Noyola,et al.  Availability of micronutrients during anaerobic digestion of molasses stellage using an Upflow Anaerobic Sludge Blanket (UASB) reactor. , 1993 .

[10]  Zonglian She,et al.  Performance evaluation of a mesophilic (37 °C) upflow anaerobic sludge blanket reactor in treating distiller's grains wastewater , 2007 .

[11]  M H Tielbaard EXPERIENCE WITH TREATMENT OF CANE VINASSE BY UASB REACTORS , 1992 .

[12]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[13]  B. Ahring,et al.  Purification of bioethanol effluent in an UASB reactor system with simultaneous biogas formation , 2003, Biotechnology and bioengineering.

[14]  S. Sung,et al.  Ammonia inhibition on thermophilic aceticlastic methanogens. , 2002, Water science and technology : a journal of the International Association on Water Pollution Research.

[15]  E. Arikawa,et al.  The Treatment of Liquor Wastewater Containing High-Strength Suspended Solids by Membrane Bioreactor System , 1992 .

[16]  Hang-sik Shin,et al.  Anaerobic Digestion of Distillery Wastewater in a Two-Phase UASB System , 1992 .

[17]  D. Ranade,et al.  Evaluation of the use of sodium molybdate to inhibit sulphate reduction during anaerobic digestion of distillery waste , 1999 .

[18]  Anne Belinda Thomsen,et al.  Hydrothermal treatment of wheat straw at pilot plant scale using a three-step reactor system aiming at high hemicellulose recovery, high cellulose digestibility and low lignin hydrolysis. , 2008, Bioresource technology.

[19]  B. Ahring,et al.  Potential inhibitors from wet oxidation of wheat straw and their effect on growth and ethanol production by Thermoanaerobacter mathranii , 2001, Applied Microbiology and Biotechnology.

[20]  G. Lettinga,et al.  The methanogenic toxicity of wastewater lignins and lignin related compounds. , 2007 .

[21]  G. Lettinga,et al.  Overview of the Anaerobic Toxicity Caused by Organic Forest Industry Wastewater Pollutants , 1994 .

[22]  P. Kaparaju,et al.  Bioethanol, biohydrogen and biogas production from wheat straw in a biorefinery concept. , 2009, Bioresource technology.

[23]  A. V. Haandel,et al.  Profitability Increase of Alcohol Distilleries by the Rational Use of Byproducts , 1994 .

[24]  M. Wentzel,et al.  Treatment of grain distillation wastewaters in an upflow anaerobic sludge bed (UASB) system , 2001 .

[25]  Igor Bodík,et al.  Anaerobic treatment of wheat stillage , 2003 .