Comparison of structural characteristics and immunoregulatory activities of polysaccharides from four natural plants

ABSTRACT Polysaccharides have various bioactivities including anti-tumour, antioxidant and immunoregulation, which are correlated with their structural characteristics. Here, four polysaccharides from Glycyrrhiza uralensis (GUPS), Pleurotus ferulae (PFPS), Triticum aestivum (TAPS) and Oryza sativa (OSPS) were purified to compare their structural characteristics and immunoregulatory activities. The data showed that TAPS, OSPS, GUPS and PFPS contained different monosaccharide components and had distinctly structural characteristics. All the four polysaccharides promoted the maturation of dendritic cells (DCs) through TLR4 with different activities. GUPS and PFPS also reduced the phagocytosis of DCs but not for TAPS and OSPS. Importantly, GUPS and PFPS enhanced the Th1 skewing immune response in mice without inflammation. TAPS and OSPS only showed weak immunostimulatory activity in vivo. The immunostimulatory activities of the four polysaccharides were ranked as PFPS = GUPS > TAPS > OSPS.

[1]  Chunjie Wu,et al.  Ultrasonic extraction, structural characterization, and bioactivities of nonstarch polysaccharides from red yeast rice , 2020, Biotechnology and applied biochemistry.

[2]  Jinyao Li,et al.  The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis , 2020, PeerJ.

[3]  Xinguo Su,et al.  Gynostemma pentaphyllum polysaccharide prevents the growth of h22 ascites tumour by enhancing immunity rather than cytotoxicity in mice , 2020 .

[4]  T. Ying,et al.  Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis , 2020, Biomolecules.

[5]  A. Lovegrove,et al.  Comparative prebiotic activity of mixtures of cereal grain polysaccharides , 2019, AMB Express.

[6]  Narpinder Singh,et al.  Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology. , 2019, Carbohydrate polymers.

[7]  Gang Liu,et al.  Suppression of NLRP3 and NF-κB signaling pathways by α-Cyperone via activating SIRT1 contributes to attenuation of LPS-induced acute lung injury in mice. , 2019, International immunopharmacology.

[8]  Jinyao Li,et al.  Pleurotus ferulae polysaccharides improve the antitumor efficacy of therapeutic human papillomavirus dendritic cell-based vaccine , 2019, Human vaccines & immunotherapeutics.

[9]  Zhenguang Liu,et al.  Mechanism of Lycium barbarum polysaccharides liposomes on activating murine dendritic cells. , 2019, Carbohydrate polymers.

[10]  Huahua Yu,et al.  Hydroxypropyltrimethyl ammonium chloride chitosan activates RAW 264.7 macrophages through the MAPK and JAK-STAT signaling pathways. , 2019, Carbohydrate polymers.

[11]  Jinyao Li,et al.  Chemical composition, antioxidant and antitumor activities of sub-fractions of wild and cultivated Pleurotus ferulae ethanol extracts , 2018, PeerJ.

[12]  Qiuhong Wang,et al.  Structural characterization and immunomodulatory activity of a pectic polysaccharide (CALB-4) from Fructus aurantii. , 2018, International journal of biological macromolecules.

[13]  Afiya John,et al.  The structure changes of water-soluble polysaccharides in papaya during ripening. , 2018, International journal of biological macromolecules.

[14]  Ying Wu,et al.  Structure characterization of two functional polysaccharides from Polygonum multiflorum and its immunomodulatory. , 2018, International journal of biological macromolecules.

[15]  Chongde Sun,et al.  Characteristics and immune-enhancing activity of pectic polysaccharides from sweet cherry (Prunus avium). , 2018, Food chemistry.

[16]  G. Mao,et al.  Purification, characterization and immunomodulatory activity of a novel polysaccharide from Grifola frondosa. , 2018, International journal of biological macromolecules.

[17]  A. Bouraoui,et al.  Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities. , 2018, Carbohydrate polymers.

[18]  L. Cai,et al.  Structural characterization, antioxidant and hepatoprotective activities of polysaccharides from Sophorae tonkinensis Radix. , 2018, Carbohydrate polymers.

[19]  Yu Yang,et al.  Characterization, antioxidant and hypoglycemic activities of degraded polysaccharides from blackcurrant (Ribes nigrum L.) fruits. , 2018, Food chemistry.

[20]  Yue Yu,et al.  Biological activities and pharmaceutical applications of polysaccharide from natural resources: A review. , 2018, Carbohydrate polymers.

[21]  Z. Dong,et al.  Structure characterization of two novel polysaccharides from Colocasia esculenta (taro) and a comparative study of their immunomodulatory activities , 2018 .

[22]  Xiaozhen Tang,et al.  Structural characterization and antioxidant activity of polysaccharide from ginger. , 2018, International journal of biological macromolecules.

[23]  Hamideh Molaei,et al.  Structural features of a new water-soluble polysaccharide from the gum exudates of Amygdalus scoparia Spach (Zedo gum). , 2018, Carbohydrate polymers.

[24]  Chong Qi,et al.  Immune-enhancing effects of a polysaccharide PRG1-1 from Russula griseocarnosa on RAW264.7 macrophage cells via the MAPK and NF-κB signalling pathways , 2018 .

[25]  R. Linhardt,et al.  Extraction and characterization of RG-I enriched pectic polysaccharides from mandarin citrus peel , 2017, Food Hydrocolloids.

[26]  Yu Yang,et al.  Sulfated modification of the polysaccharides from blackcurrant and their antioxidant and α-amylase inhibitory activities. , 2017, International journal of biological macromolecules.

[27]  Juan Yu,et al.  Characterization of Se-enriched Pleurotus ostreatus polysaccharides and their antioxidant effects in vitro. , 2017, International journal of biological macromolecules.

[28]  Junhui Wang,et al.  Structural elucidation of a pectin-type polysaccharide from Hovenia dulcis peduncles and its proliferative activity on RAW264.7 cells. , 2017, International journal of biological macromolecules.

[29]  Yuanliang Hu,et al.  Optimization of Glycyrrhiza polysaccharide liposome by response surface methodology and its immune activities. , 2017, International journal of biological macromolecules.

[30]  C. Gomes,et al.  Dendritic cell-based immunotherapy: a basic review and recent advances , 2017, Immunologic Research.

[31]  Xi-chen Zhang,et al.  Docosahexaenoic acid attenuates LPS-stimulated inflammatory response by regulating the PPARγ/NF-κB pathways in primary bovine mammary epithelial cells. , 2017, Research in veterinary science.

[32]  T. Ying,et al.  Purification, characterization and bioactivities of polysaccharides from Pleurotus ferulae. , 2017, Food & function.

[33]  S. You,et al.  Structural effects of sulfated polysaccharides from Codium fragile on NK cell activation and cytotoxicity. , 2017, International journal of biological macromolecules.

[34]  Jianhua Yang,et al.  Glycyrrhiza uralensis water extract enhances dendritic cell maturation and antitumor efficacy of HPV dendritic cell-based vaccine , 2017, Scientific Reports.

[35]  Q. Hu,et al.  Preparation, characterization, and anti-inflammatory effect of the chelate of Flammulina velutipes polysaccharide with Zn , 2017 .

[36]  L. Yang,et al.  Effect of Glycyrrhiza uralensis Fisch polysaccharide on growth performance and immunologic function in mice in Ural City, Xinjiang. , 2016, Asian Pacific journal of tropical medicine.

[37]  H. Kuang,et al.  Anti-diabetic polysaccharides from natural sources: A review. , 2016, Carbohydrate polymers.

[38]  Juping Yu,et al.  Structure characterization, chemical and enzymatic degradation, and chain conformation of an acidic polysaccharide from Lycium barbarum L. , 2016, Carbohydrate polymers.

[39]  Fan Zhang,et al.  EPSAH, an exopolysaccharide from Aphanothece halophytica GR02, improves both cellular and humoral immunity as a novel polysaccharide adjuvant. , 2016, Chinese journal of natural medicines.

[40]  Jinyao Li,et al.  The combination of Pleurotus ferulae water extract and CpG-ODN enhances the immune responses and antitumor efficacy of HPV peptides pulsed dendritic cell-based vaccine. , 2016, Vaccine.

[41]  Jin-jian Lu,et al.  A Systematic Review of the Anticancer Properties of Compounds Isolated from Licorice (Gancao) , 2015, Planta Medica.

[42]  M. Coimbra,et al.  Structure-function relationships of immunostimulatory polysaccharides: A review. , 2015, Carbohydrate polymers.

[43]  Jinyao Li,et al.  Pleurotus ferulae water extract enhances the maturation and function of murine bone marrow-derived dendritic cells through TLR4 signaling pathway. , 2015, Vaccine.

[44]  Jingkun Yan,et al.  Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides. , 2014, Carbohydrate polymers.

[45]  Zhanyong Wang,et al.  Antioxidant and immunological activity in vitro of polysaccharides from Phellinus nigricans mycelia. , 2014, International journal of biological macromolecules.

[46]  C. Soccol,et al.  Agaricus brasiliensis mycelium and its polysaccharide modulate the parameters of innate and adaptive immunity , 2013 .

[47]  Guangli Yu,et al.  Carrageenan from Solieria chordalis (Gigartinales): Structural analysis and immunological activities of the low molecular weight fractions , 2010 .

[48]  Xiu‐ping Dong,et al.  Purification and bioactivity of a sulphated polysaccharide conjugate from viscera of abalone Haliotis discus hannai Ino , 2010 .

[49]  Hongxiang Sun,et al.  Novel polysaccharide adjuvant from the roots of Actinidia eriantha with dual Th1 and Th2 potentiating activity. , 2009, Vaccine.

[50]  Hossein Hosseinzadeh,et al.  Review of Pharmacological Effects of Glycyrrhiza sp. and its Bioactive Compounds , 2008, Phytotherapy research : PTR.

[51]  M. Quinn,et al.  Botanical polysaccharides: macrophage immunomodulation and therapeutic potential. , 2006, International immunopharmacology.

[52]  Zhien Li,et al.  In vivo antitumor and immunomodulation activities of different molecular weight lambda-carrageenans from Chondrus ocellatus. , 2004, Pharmacological research.

[53]  L. Ryan,et al.  The cytokine stimulating activity of (1-->3)-beta-D-glucans is dependent on the triple helix conformation. , 2000, Carbohydrate research.

[54]  T. Lee,et al.  Consequence of the antioxidant activities and tyrosinase inhibitory effects of various extracts from the fruiting bodies of Pleurotus ferulae. , 2012, Saudi journal of biological sciences.

[55]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .