Identification of Fecal Escherichia colifrom Humans and Animals by Ribotyping

ABSTRACT Fecal pollution of water resources is an environmental problem of increasing importance. Identification of individual host sources of fecal Escherichia coli, such as humans, pets, production animals, and wild animals, is prerequisite to formulation of remediation plans. Ribotyping has been used to distinguish fecalE. coli of human origin from pooled fecal E. coli isolates of nonhuman origin. We have extended application of this technique to distinguishing fecal E. coli ribotype patterns from human and seven individual nonhuman hosts. Classification accuracy was best when the analysis was limited to three host sources. Application of this technique to identification of host sources of fecal coliforms in water could assist in formulation of pollution reduction plans.

[1]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[2]  T. C. Daniel,et al.  Runoff Water Quality from Poultry Litter-Treated Pasture and Forest Sites , 2000 .

[3]  Katharine G. Field,et al.  A PCR Assay To Discriminate Human and Ruminant Feces on the Basis of Host Differences in Bacteroides-Prevotella Genes Encoding 16S rRNA , 2000, Applied and Environmental Microbiology.

[4]  F. Grimont,et al.  Universal ribotyping method using a chemically labelled oligonucleotide probe mixture. , 1997, Research in microbiology.

[5]  Kenneth M. Portier,et al.  Discriminant Analysis of Ribotype Profiles of Escherichia coli for Differentiating Human and Nonhuman Sources of Fecal Pollution , 1999, Applied and Environmental Microbiology.

[6]  Awwa,et al.  Standard Methods for the examination of water and wastewater , 1999 .

[7]  B A Wiggins,et al.  Discriminant analysis of antibiotic resistance patterns in fecal streptococci, a method to differentiate human and animal sources of fecal pollution in natural waters , 1996, Applied and environmental microbiology.

[8]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[9]  C. Hart,et al.  Genotype Analysis of Escherichia coliStrains Isolated from Children and Chickens Living in Close Contact , 1999, Applied and Environmental Microbiology.

[10]  F Jones,et al.  Marine waters contaminated with domestic sewage: nonenteric illnesses associated with bather exposure in the United Kingdom. , 1996, American journal of public health.

[11]  U. Schibler,et al.  Changes in size and secondary structure of the ribosomal transcription unit during vertebrate evolution. , 1975, Journal of molecular biology.

[12]  Michael J. Sadowsky,et al.  Use of Repetitive DNA Sequences and the PCR To DifferentiateEscherichia coli Isolates from Human and Animal Sources , 2000, Applied and Environmental Microbiology.

[13]  R. B. Reneau,et al.  Determining Sources of Fecal Pollution in a Rural Virginia Watershed with Antibiotic Resistance Patterns in Fecal Streptococci , 1999, Applied and Environmental Microbiology.

[14]  V. Harwood,et al.  Classification of Antibiotic Resistance Patterns of Indicator Bacteria by Discriminant Analysis: Use in Predicting the Source of Fecal Contamination in Subtropical Waters , 2000, Applied and Environmental Microbiology.

[15]  R. A. Conway,et al.  Use of Antibiotic Resistance Analysis To Identify Nonpoint Sources of Fecal Pollution , 1999, Applied and Environmental Microbiology.

[16]  E. A. Robertson,et al.  Computer analysis of Staphylococcus aureus phage typing data from 1957 to 1975, citing epidemiological trends and natural evolution within the phage typing system , 1980, Applied and environmental microbiology.

[17]  K. Portier,et al.  Association of multiple-antibiotic-resistance profiles with point and nonpoint sources of Escherichia coli in Apalachicola Bay , 1997, Applied and environmental microbiology.

[18]  Geldreich Ee,et al.  Concepts of fecal streptococci in stream pollution. , 1969 .

[19]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.