Quantitative enumeration of acanthamoeba for evaluation of cyst inactivation in contact lens care solutions.

A simple, quantitative plate assay has been developed for use in the enumeration of Acanthamoeba. The technique uses an agarose overlay and low-nutrient medium to support the growth of amoebae on a bacterial lawn. The authors found that in this assay, individual trophozoites or dormant cysts will cause plaques to form in an Enterobacter aerogenes lawn. With the assay, it is possible to quantitatively assess the effects of various disinfectant compounds on the viability of Acanthamoeba. The authors used this assay to enumerate Acanthamoeba cyst viability after chemical disinfection in contact lens care solutions. The inactivation data indicated major differences among the four test solutions evaluated.

[1]  C. Anger,et al.  A quantitative bacterial plaque assay for the enumeration of viable Acanthamoeba cells. , 1991, Reviews of infectious diseases.

[2]  M. B. Moore,et al.  The effect of currently available contact lens disinfection systems on Acanthamoeba castellanii and Acanthamoeba polyphaga. , 1990, Ophthalmology.

[3]  G. Visvesvara,et al.  Viability of Acanthamoeba cysts in ophthalmic solutions , 1989, Applied and environmental microbiology.

[4]  D. Bruckner,et al.  Microbial contamination of contact lens care systems. , 1987, American journal of ophthalmology.

[5]  D. Meisler,et al.  Susceptibility of Acanthamoeba to soft contact lens disinfection systems. , 1986, Investigative ophthalmology & visual science.

[6]  M. B. Moore,et al.  Acanthamoeba keratitis associated with soft contact lenses. , 1985, American journal of ophthalmology.

[7]  R. L. Font,et al.  Acanthamoeba keratitis possibly acquired from a hot tub. , 1984, Archives of ophthalmology.

[8]  G. Dubes,et al.  Cloning, titration, and differentiation of Acanthamoeba sp. by plating. , 1962, The Journal of parasitology.

[9]  R. J. Neff.  Purification, axenic cultivation, and description of a soil amoeba, Acanthamoeba sp , 1957 .

[10]  M. Sussman,et al.  Cultivation and synchronous morphogenesis of Dictyostelium under controlled experimental conditions. , 1987, Methods in cell biology.

[11]  R. J. Neff,et al.  Chapter 4 Induction of Synchronous Encystment (Differentiation) in Acanthamoeba sp. , 1964 .