A fast MEMS scanning photoacoustic microscopy system and its application in glioma study

We present a water-proof Microelectromechanical systems (MEMS) based scanning optical resolution Photoacoustic Microscopy (OR-PAM) system and its application in glioma tumor mouse model study. The presented OR-PAM system has high optical resolution (~3 μm) and high scanning speed (up to 50 kHz A-scan rate), which is ideal for cerebral vascular imaging. In this study, the mice with glioma tumor are treated with vascular disrupting agent (VDA). OR-PAM system is utilized to image the cerebral with the whole skull intact before and after the injection of VDA. By image registration, the response of every single blood vessel can be traced. This will provide us deeper understanding of the drug effect.

[1]  Kijoon Lee,et al.  Deep tissue flowmetry based on diffuse speckle contrast analysis. , 2013, Optics letters.

[2]  Yun Wu,et al.  Review on photoacoustic imaging of the brain using nanoprobes , 2016, Neurophotonics.

[3]  Lihong V Wang,et al.  Photoacoustic microscopy and computed tomography: from bench to bedside. , 2014, Annual review of biomedical engineering.

[4]  Anwar R. Padhani,et al.  Perfusion MRI in the early clinical development of antivascular drugs: decorations or decision making tools? , 2010, European Journal of Nuclear Medicine and Molecular Imaging.

[5]  Chulhong Kim,et al.  In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach , 2017, Scientific Reports.

[6]  M. Seshadri,et al.  MRI-Based Characterization of Vascular Disruption by 5,6-Dimethylxanthenone-Acetic Acid in Gliomas , 2009, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[7]  Chulhong Kim,et al.  A PDMS-Based 2-Axis Waterproof Scanner for Photoacoustic Microscopy , 2015, Sensors.

[8]  R R Alfano,et al.  Spectral Polarization Imaging of Human Prostate Cancer Tissue Using a Near-infrared Receptor-targeted Contrast Agent , 2005, Technology in cancer research & treatment.

[9]  Jun Zou,et al.  Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror. , 2012, Journal of biomedical optics.

[10]  Kijoon Lee,et al.  Diffuse correlation spectroscopy with a fast Fourier transform-based software autocorrelator , 2012, Journal of biomedical optics.

[11]  Jin Young Kim,et al.  Fast optical-resolution photoacoustic microscopy using a 2-axis water-proofing MEMS scanner , 2015, Scientific Reports.

[12]  Lihong V. Wang,et al.  A practical guide to photoacoustic tomography in the life sciences , 2016, Nature Methods.

[13]  Lihong V. Wang,et al.  High-speed label-free functional photoacoustic microscopy of mouse brain in action , 2015, Nature Methods.

[14]  R. Jenkins,et al.  Genetics of adult glioma. , 2012, Cancer genetics.

[15]  Vasilis Ntziachristos,et al.  Multispectral optoacoustic and MRI coregistration for molecular imaging of orthotopic model of human glioblastoma , 2016, Journal of biophotonics.

[16]  K. Soo,et al.  Hemodynamic monitoring of Chlorin e6-mediated photodynamic therapy using diffuse optical measurements. , 2014, Journal of photochemistry and photobiology. B, Biology.

[17]  Chulhong Kim,et al.  Looking Deeper: Multimodal and contrast-enhanced photoacoustic imaging offer a clearer view within tissues for more accurate diagnosis. , 2015, IEEE Pulse.

[18]  Yang Pu,et al.  Synthesis of dye conjugates to visualize the cancer cells using fluorescence microscopy. , 2014, Applied optics.

[19]  Lihong V. Wang,et al.  Optical-resolution photoacoustic microscopy: auscultation of biological systems at the cellular level. , 2013, Biophysical journal.

[20]  F. Lee,et al.  Effects of combretastatin A4 phosphate on endothelial cell morphology in vitro and relationship to tumour vascular targeting activity in vivo. , 2001, Anticancer research.

[21]  Kijoon Lee,et al.  Optical methods for blood perfusion measurement--theoretical comparison among four different modalities. , 2015, Journal of the Optical Society of America. A, Optics, image science, and vision.

[22]  Chris Jun Hui Ho,et al.  Noninvasive real-time characterization of non-melanoma skin cancers with handheld optoacoustic probes , 2017, Photoacoustics.

[23]  Junjie Yao,et al.  Photoacoustic microscopy , 2013, Laser & photonics reviews.

[24]  M. Olivo,et al.  Photoacoustic microscopy for evaluating combretastatin A4 phosphate induced vascular disruption in orthotopic glioma , 2018, Journal of biophotonics.

[25]  Jin Young Kim,et al.  Handheld Photoacoustic Microscopy Probe , 2017, Scientific Reports.