Pyrosequencing analysis of eukaryotic and bacterial communities in faucet biofilms.

In order to understand the microbial communities in drinking water biofilms, both eukaryotic and bacterial communities in three faucet biofilms were characterized by 454 pyrosequencing and quantitative PCR approaches. Microbial assemblages of the biofilms were dominated by bacteria, with Sphingomonadales, Rhizobiales, and Burkholderiales comprising the major bacterial populations. Although about 2 years of biofilm development occurred, the microbial community at site WSW still demonstrates the characteristics of a young biofilm community, e.g. low biomass, abundant aggregating bacteria (Blastomonas spp. and Acidovorax spp.) etc. Hartmannella of amoebae was the dominant eukaryotic predator in the biofilms, and correlated closely with biofilm bacterial biomass. Nonetheless, there was no obvious association of pathogens with amoebae in the faucet biofilms. In contrast, residual chlorine seems to be a dominant factor impacting the abundance of Legionella and Mycobacterium, two primary potential opportunistic pathogens detected in all faucet biofilms.

[1]  F. Portaels,et al.  Mycobacteria in drinking water distribution systems: ecology and significance for human health. , 2005, FEMS microbiology reviews.

[2]  R. Knight,et al.  A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses , 2009, The ISME Journal.

[3]  N. High,et al.  Coaggregation between Aquatic Bacteria Is Mediated by Specific-Growth-Phase-Dependent Lectin-Saccharide Interactions , 2000, Applied and Environmental Microbiology.

[4]  D. Raoult,et al.  Survival of Environmental Mycobacteria in Acanthamoeba polyphaga , 2006, Applied and Environmental Microbiology.

[5]  D. Stahl,et al.  Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens , 1994, Applied and environmental microbiology.

[6]  G. Ozolins,et al.  WHO guidelines for drinking-water quality. , 1984, WHO chronicle.

[7]  K. Goto,et al.  Identification of yellow-pigmented bacteria isolated from hospital tap water in Japan and their chlorine resistance. , 2007, Biocontrol science.

[8]  M. Jung,et al.  Comparative Analysis of Korean Human Gut Microbiota by Barcoded Pyrosequencing , 2011, PloS one.

[9]  D. van der Kooij,et al.  Relationships between Free-Living Protozoa, Cultivable Legionella spp., and Water Quality Characteristics in Three Drinking Water Supplies in the Caribbean , 2011, Applied and Environmental Microbiology.

[10]  K. Jakobsen,et al.  Freshwater Perkinsea and marine-freshwater colonizations revealed by pyrosequencing and phylogeny of environmental rDNA , 2010, The ISME Journal.

[11]  P. Servais,et al.  Bacterial dynamics in the drinking water distribution system of Brussels. , 2001, Water research.

[12]  S. Percival,et al.  Potable water and biofilms: A review of the public health implications , 1999 .

[13]  S. Goodison,et al.  16S ribosomal DNA amplification for phylogenetic study , 1991, Journal of bacteriology.

[14]  M. W. Kuiper,et al.  Quantitative Detection of the Free-Living Amoeba Hartmannella vermiformis in Surface Water by Using Real-Time PCR , 2006, Applied and Environmental Microbiology.

[15]  M. Vieira,et al.  Incorporation of natural uncultivable Legionella pneumophila into potable water biofilms provides a protective niche against chlorination stress , 2009, Biofouling.

[16]  Min Yang,et al.  Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems. , 2012, Canadian journal of microbiology.

[17]  M. Vieira,et al.  Intergeneric Coaggregation among Drinking Water Bacteria: Evidence of a Role for Acinetobacter calcoaceticus as a Bridging Bacterium , 2007, Applied and Environmental Microbiology.

[18]  Leah M. Feazel,et al.  Opportunistic pathogens enriched in showerhead biofilms , 2009, Proceedings of the National Academy of Sciences.

[19]  John M. Regan,et al.  Ammonia- and Nitrite-Oxidizing Bacterial Communities in a Pilot-Scale Chloraminated Drinking Water Distribution System , 2002, Applied and Environmental Microbiology.

[20]  Andrew J. McBain,et al.  Shear Rate Moderates Community Diversity in Freshwater Biofilms , 2004, Applied and Environmental Microbiology.

[21]  M. Meckes,et al.  Phylogenetic diversity of drinking water bacteria in a distribution system simulator , 2004, Journal of applied microbiology.

[22]  J. Parry,et al.  The effect of substratum roughness and river flow rate on the development of a freshwater biofilm community , 1998 .

[23]  S. Molin,et al.  Long-Term Succession of Structure and Diversity of a Biofilm Formed in a Model Drinking Water Distribution System , 2003, Applied and Environmental Microbiology.

[24]  Karsten Pedersen,et al.  Biofilm development on stainless steel and PVC surfaces in drinking water , 1990 .

[25]  W. Ludwig,et al.  SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB , 2007, Nucleic acids research.

[26]  Lutgarde Raskin,et al.  Microbial ecology of drinking water distribution systems. , 2006, Current opinion in biotechnology.

[27]  D. White,et al.  The genus Sphingomonas: physiology and ecology. , 1996, Current opinion in biotechnology.

[28]  M. W. Kuiper,et al.  Occurrence of Legionella pneumophila and Hartmannella vermiformis in fresh water environments and their interactions in biofilms , 2006 .

[29]  M. W. Kuiper,et al.  Intracellular Proliferation of Legionella pneumophila in Hartmannella vermiformis in Aquatic Biofilms Grown on Plasticized Polyvinyl Chloride , 2004, Applied and Environmental Microbiology.

[30]  P. Marsh,et al.  Extended Survival and Persistence of Campylobacterspp. in Water and Aquatic Biofilms and Their Detection by Immunofluorescent-Antibody and -rRNA Staining , 1998, Applied and Environmental Microbiology.

[31]  M. Höfle,et al.  Composition and Dynamics of Bacterial Communities of a Drinking Water Supply System as Assessed by RNA- and DNA-Based 16S rRNA Gene Fingerprinting , 2006, Applied and Environmental Microbiology.

[32]  U. Szewzyk,et al.  In situ probing reveals Aquabacterium commune as a widespread and highly abundant bacterial species in drinking water biofilms , 2000 .

[33]  Mark W. LeChevallier,et al.  Pyrosequencing Analysis of Bacterial Biofilm Communities in Water Meters of a Drinking Water Distribution System , 2010, Applied and Environmental Microbiology.

[34]  J. S. Santo Domingo,et al.  Identification of active bacterial communities in a model drinking water biofilm system using 16S rRNA-based clone libraries. , 2006, FEMS microbiology letters.

[35]  J. Bae,et al.  Investigation of archaeal and bacterial diversity in fermented seafood using barcoded pyrosequencing , 2010, The ISME Journal.

[36]  D. Lane.  16S/23S rRNA sequencing , 1991 .

[37]  Ramon G. Lee,et al.  Bacterial nutrients in drinking water , 1991, Applied and environmental microbiology.

[38]  Dong Li,et al.  Characterization of Bacterial Community Structure in a Drinking Water Distribution System during an Occurrence of Red Water , 2010, Applied and Environmental Microbiology.

[39]  Jorge W Santo Domingo,et al.  Identification of bacterial populations in drinking water using 16S rRNA-based sequence analyses. , 2010, Water research.

[40]  H. Smidt,et al.  Free-Living Protozoa in Two Unchlorinated Drinking Water Supplies, Identified by Phylogenic Analysis of 18S rRNA Gene Sequences , 2009, Applied and Environmental Microbiology.

[41]  J. Wingender,et al.  Metagenome Survey of Biofilms in Drinking-Water Networks , 2003, Applied and Environmental Microbiology.

[42]  S. Molin,et al.  Identification of Bacteria in Biofilm and Bulk Water Samples from a Nonchlorinated Model Drinking Water Distribution System: Detection of a Large Nitrite-Oxidizing Population Associated with Nitrospira spp , 2005, Applied and Environmental Microbiology.

[43]  M. Pachiadaki,et al.  Changes of the bacterial assemblages throughout an urban drinking water distribution system , 2010, Environmental monitoring and assessment.

[44]  Tong Zhang,et al.  Pathogenic bacteria in sewage treatment plants as revealed by 454 pyrosequencing. , 2011, Environmental science & technology.

[45]  T. Cavalier-smith,et al.  Helkesimastix marina n. sp. (Cercozoa: Sainouroidea superfam. n.) a gliding zooflagellate of novel ultrastructure and unusual ciliary behaviour. , 2009, Protist.

[46]  P. Borella,et al.  Ecological behaviour of three serogroups of Legionella pneumophila within a model plumbing system , 2011, Biofouling.

[47]  P. Martikainen,et al.  Occurrence of nitrifiers and diversity of ammonia-oxidizing bacteria in developing drinking water biofilms. , 2004, Water research.

[48]  J. Regan,et al.  Diversity of nitrifying bacteria in full-scale chloraminated distribution systems. , 2003, Water research.

[49]  Martin Hartmann,et al.  Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities , 2009, Applied and Environmental Microbiology.

[50]  Dong-geun Lee,et al.  Influence of pipe materials and VBNC cells on culturable bacteria in a chlorinated drinking water model system. , 2007, Journal of microbiology and biotechnology.

[51]  P. Laurent,et al.  Biofilm responses to ageing and to a high phosphate load in a bench-scale drinking water system. , 2003, Water research.