AIM
Microbiota dysbiosis in inflammatory bowel disease (IBD) have been widely reported. Gut microbiota connect diet to metabolism by producing small molecules through diverse metabolic pathways. However, little is known about the correlation among metabolic activity, microbiota composition, and dietary components in IBD.
METHODS
We investigated the dietary preferences of patients in an IBD cohort (including ulcerative colitis (UC) and Crohn's disease (CD) patients) and analyzed their gut microbiota using 16S rRNA gene sequencing and metagenomic analysis of fecal and biopsy samples. We then examined the metabolite profiles of the samples using gas and liquid chromatography-mass spectrometry analyses.
RESULTS
Daily intakes of folic acid, niacin, vitamin C, vitamin D, calcium, and selenium differed significantly between IBD and healthy controls. Detection of metabolites indicated that the decrease of long-chain fatty acids (arachidic acid, and oleic acid et al.), medium-chain fatty acids (sebacic acid and isocaproic acid), and bile acid in IBD patients. Compared with healthy people, 21 microbial species (such as Sulfolobus acidocaldarius, and Clostridium clostridioforme CAG132 et al.) were identified to correlate to diet and metabolites in UC group, and the number is 37 in CD group (such as Bacteroides fragilis, and Fusobacterium nucleatum et al.). Bacteroides fragilis, which was an example, enriched in IBD patients and associated with multi-nutrients, and 21 metabolites including 25-hydroxyvitamin D3 and taurolithocholic acid.
CONCLUSIONS
This study provides a network to identify key micronutrients, microbiota components and metabolites that contribute to IBD. This article is protected by copyright. All rights reserved.