Micro patterning of cell and protein non-adhesive plasma polymerized coatings for biochip applications.

Micro scale patterning of bioactive surfaces is desirable for numerous biochip applications. Polyethyleneoxide-like (PEO-like) coating with non-fouling functionality has been deposited using low frequency AC plasma polymerization. The non-fouling properties of the coating were tested with human cells (HeLa) and fluorescence labeled proteins (isothiocyanate-labeled bovine serum albumin, i.e. FITC-BSA). The PEO-like coatings were fabricated by plasma polymerization of 12-crown-4 (ppCrown) with plasma polymerized hexene (ppHexene) as adhesion layer. The coatings were micro patterned using conventional cleanroom photolithography and lift-off. Single cell arrays showed sharp contrast in cell adhesion between the untreated glass surface and the ppCrown layer. Similarly, proteins adsorbed selectively to untreated glass but not to ppCrown. The simplicity of the lift-off technique and the sturdiness and versatility of the plasma-polymerized coatings, make this technology highly suitable for bio-MEMS and biochip applications, where patterned high contrast non-fouling surfaces are needed.

[1]  K E Healy,et al.  Spatial distribution of mammalian cells dictated by material surface chemistry , 1994, Biotechnology and bioengineering.

[2]  George M. Whitesides,et al.  Patterning Self-Assembled Monolayers: Applications in Materials Science , 1994 .

[3]  Daniel I. C. Wang,et al.  Engineering cell shape and function. , 1994, Science.

[4]  Sheyu Guo,et al.  DC plasma polymerization of hexamethyldisiloxane , 1997 .

[5]  D E Ingber,et al.  Using microcontact printing to pattern the attachment of mammalian cells to self-assembled monolayers of alkanethiolates on transparent films of gold and silver. , 1997, Experimental cell research.

[6]  C. S. Chen,et al.  Geometric control of cell life and death. , 1997, Science.

[7]  P. Rouxhet,et al.  Adhesion of mammalian cells to polymer surfaces: from physical chemistry of surfaces to selective adhesion on defined patterns. , 1998, Biomaterials.

[8]  Conrad D. James,et al.  Patterned Protein Layers on Solid Substrates by Thin Stamp Microcontact Printing , 1998 .

[9]  Milan Mrksich,et al.  Micropatterned Surfaces for Control of Cell Shape, Position, and Function , 1998, Biotechnology progress.

[10]  S. Shirley,et al.  Biological Application of Microstructures , 1998 .

[11]  Gregory T. A. Kovacs,et al.  Cell-based sensor microelectrode array characterized by imaging x-ray photoelectron spectroscopy, scanning electron microscopy, impedance measurements, and extracellular recordings , 1998 .

[12]  Frances S. Ligler,et al.  An Array Immunosensor for Simultaneous Detection of Clinical Analytes , 1999 .

[13]  K E Healy,et al.  Surfaces designed to control the projected area and shape of individual cells. , 1999, Journal of biomechanical engineering.

[14]  Hiroyuki Fujita,et al.  A Silicon Shadow Mask with Unlimited Patterns and a Mechanical Alignment Structure by Al-Delay Masking Process , 2000 .

[15]  Timmons,et al.  Non-fouling surfaces produced by gas phase pulsed plasma polymerization of an ultra low molecular weight ethylene oxide containing monomer. , 2000, Colloids and surfaces. B, Biointerfaces.

[16]  H. Fujita,et al.  A silicon shadow mask for deposition on isolated areas , 2000 .

[17]  D. D. Denton,et al.  Micromachining of non-fouling coatings for bio-MEMS applications , 2001 .

[18]  Andrew J. Griggs,et al.  Pulsed Plasma Polymerization of Cyclic Ethers: Production of Biologically Nonfouling Surfaces , 2001 .

[19]  T. W. Schneider,et al.  Surface Patterning and Adhesion of Neuroblastoma X Glioma (NG108-15) Cells , 2001 .

[20]  Louis Tiefenauer,et al.  Photolithographic generation of protein micropatterns for neuron culture applications. , 2002, Biomaterials.

[21]  Todd C. McDevitt,et al.  Micro-Scale Cell Patterning on Nonfouling Plasma Polymerized Tetraglyme Coatings by Protein Microcontact Printing , 2002 .

[22]  S. J. Kwoun,et al.  The study of a cell-based TSM piezoelectric sensor , 2002, Proceedings of the 2002 IEEE International Frequency Control Symposium and PDA Exhibition (Cat. No.02CH37234).

[23]  Jonathan M Cooper,et al.  Micromachined nanocalorimetric sensor for ultra-low-volume cell-based assays. , 2002, Analytical chemistry.

[24]  M. Morra,et al.  Cell Adhesion Micropatterning by Plasma Treatment of Alginate Coated Surfaces , 2002 .

[25]  Mandana Veiseh,et al.  Highly Selective Protein Patterning on Gold−Silicon Substrates for Biosensor Applications , 2002 .

[26]  Byung-Gee Kim,et al.  Protein patterning on silicon-based surface using background hydrophobic thin film. , 2003, Biosensors & bioelectronics.

[27]  F. Dickert,et al.  Nano- and micro-structuring of sensor materials—from molecule to cell detection , 2003 .

[28]  T. H. Rider,et al.  A B Cell-Based Sensor for Rapid Identification of Pathogens , 2003, Science.

[29]  Yong-Kweon Kim,et al.  Protein patterning by virtual mask photolithography using a micromirror array , 2003 .

[30]  P. Favia,et al.  Novel plasma processes for biomaterials: micro-scale patterning of biomedical polymers , 2003 .

[31]  Wei He,et al.  Lithography application of a novel photoresist for patterning of cells. , 2004, Biomaterials.

[32]  Jacques Jonsmann,et al.  Plasma-polymerized coatings for bio-MEMS applications , 2004 .

[33]  Ali Khademhosseini,et al.  A simple soft lithographic route to fabrication of poly(ethylene glycol) microstructures for protein and cell patterning. , 2004, Biomaterials.