Efficient method for functionalization of carbon nanotubes by lysine and improved antimicrobial activity and water-dispersion

Abstract Here, multi-walled carbon nanotubes (CNT) were first functionalized with lysine under microwave irradiation. The water-dispersed CNT were obtained by diazonium-assisted functionalization. Formation of lysine on CNTs surface was confirmed by FTIR, TGA, Raman and TEM techniques. Then, by minimal inhibitory concentration (MIC), antimicrobial activity of functionalized and pristine CNT was studied on three Gram-negative bacteria as well as three Gram-positive bacteria. The MIC results show that functionalized CNT with lysine were more effective than pristine CNT against all studied bacteria. This simple and efficient mechanism could be used to CNT functionalization with molecules containing primary amine groups.

[1]  Hongwei Zhou,et al.  Efficient direct water dispersion of multi-walled carbon nanotubes by functionalization with lysine , 2007 .

[2]  S. V. Grachev,et al.  Interaction of Gram-negative bacteria with cationic proteins: Dependence on the surface characteristics of the bacterial cell , 2009, International journal of general medicine.

[3]  T. Ganz,et al.  Granulysin, a T Cell Product, Kills Bacteria by Altering Membrane Permeability1 , 2000, The Journal of Immunology.

[4]  Facundo Ruiz,et al.  Antibacterial effect of silver nanoparticles against Streptococcus mutans , 2009 .

[5]  T. Nyokong,et al.  Characterization of amine-functionalized single-walled carbon nanotube-low symmetry phthalocyanine conjugates , 2010 .

[6]  Cher Ming Tan,et al.  Antibacterial action of dispersed single-walled carbon nanotubes on Escherichia coli and Bacillus subtilis investigated by atomic force microscopy. , 2010, Nanoscale.

[7]  Ned S Wingreen,et al.  Cell shape and cell-wall organization in Gram-negative bacteria , 2008, Proceedings of the National Academy of Sciences.

[8]  Yu-hua Chen,et al.  Characterization of antimicrobial peptides isolated from the skin of the Chinese frog, Rana dybowskii. , 2009, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[9]  M. Prato,et al.  Carbon nanotubes and microwaves: interactions, responses, and applications. , 2009, ACS nano.

[10]  X. Qi,et al.  Multi‐walled carbon nanotubes/epilson‐polylysine nanocomposite with enhanced antibacterial activity , 2011, Letters in applied microbiology.

[11]  Menachem Elimelech,et al.  Antibacterial effects of carbon nanotubes: size does matter! , 2008, Langmuir : the ACS journal of surfaces and colloids.

[12]  Menachem Elimelech,et al.  Single-walled carbon nanotubes exhibit strong antimicrobial activity. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[13]  P. Gasda,et al.  Functionalization of Single-Walled Carbon Nanotubes with 1,4-Benzenediamine Using a Diazonium Reaction , 2008 .

[14]  Seoktae Kang,et al.  Antimicrobial biomaterials based on carbon nanotubes dispersed in poly(lactic-co-glycolic acid). , 2010, Nanoscale.

[15]  Saeed Zeinali Heris,et al.  One-pot, efficient functionalization of multi-walled carbon nanotubes with diamines by microwave method , 2011 .

[16]  R. Mohan,et al.  An efficient growth of silver and copper nanoparticles on multiwalled carbon nanotube with enhanced antimicrobial activity. , 2011, Journal of biomedical materials research. Part B, Applied biomaterials.