Zebrafish paraxial protocadherin is a downstream target of spadetail involved in morphogenesis of gastrula mesoderm.

Zebrafish paraxial protocadherin (papc) encodes a transmembrane cell adhesion molecule (PAPC) expressed in trunk mesoderm undergoing morphogenesis. Microinjection studies with a dominant-negative secreted construct suggest that papc is required for proper dorsal convergence movements during gastrulation. Genetic studies show that papc is a close downstream target of spadetail, gene encoding a transcription factor required for mesodermal morphogenetic movements. Further, we show that the floating head homeobox gene is required in axial mesoderm to repress the expression of both spadetail and papc, promoting notochord and blocking differentiation of paraxial mesoderm. The PAPC structural cell-surface protein may provide a link between regulatory transcription factors and the actual cell biological behaviors that execute morphogenesis during gastrulation.

[1]  C. Kimmel,et al.  Molecular identification of spadetail: regulation of zebrafish trunk and tail mesoderm formation by T-box genes. , 1998, Development.

[2]  R. Ho,et al.  Characterization of the zebrafish tbx16 gene and evolution of the vertebrate T-box family , 1998, Development Genes and Evolution.

[3]  Amy S. Espeseth,et al.  NF-protocadherin, a novel member of the cadherin superfamily, is required for Xenopus ectodermal differentiation , 1998, Current Biology.

[4]  Virginia E. Papaioannou,et al.  Three neural tubes in mouse embryos with mutations in the T-box gene Tbx6 , 1998, Nature.

[5]  O. Pourquié,et al.  Avian hairy Gene Expression Identifies a Molecular Clock Linked to Vertebrate Segmentation and Somitogenesis , 1997, Cell.

[6]  Y. Yan,et al.  Genetic interactions in zebrafish midline development. , 1997, Developmental biology.

[7]  M. Horb,et al.  A vegetally localized T-box transcription factor in Xenopus eggs specifies mesoderm and endoderm and is essential for embryonic mesoderm formation. , 1997, Development.

[8]  D. Wettstein,et al.  The Notch ligand, X-Delta-2, mediates segmentation of the paraxial mesoderm in Xenopus embryos. , 1997, Development.

[9]  D. Grunwald,et al.  tbx6, a Brachyury-related gene expressed by ventral mesendodermal precursors in the zebrafish embryo. , 1997, Developmental biology.

[10]  M. L. King,et al.  Xenopus VegT RNA is localized to the vegetal cortex during oogenesis and encodes a novel T-box transcription factor involved in mesodermal patterning. , 1996, Development.

[11]  J. Gurdon,et al.  The Xenopus T-box gene, Antipodean, encodes a vegetally localised maternal mRNA and can trigger mesoderm formation. , 1996, Development.

[12]  P. Lemaire,et al.  The vertebrate organizer: structure and molecules. , 1996, Trends in genetics : TIG.

[13]  C. Nüsslein-Volhard,et al.  Mutations affecting morphogenesis during gastrulation and tail formation in the zebrafish, Danio rerio. , 1996, Development.

[14]  C. Nüsslein-Volhard,et al.  Mutations affecting the formation of the notochord in the zebrafish, Danio rerio. , 1996, Development.

[15]  M. Kirschner,et al.  Expression cloning of a Xenopus T-related gene (Xombi) involved in mesodermal patterning and blastopore lip formation. , 1996, Development.

[16]  B. Gumbiner,et al.  Lateral dimerization is required for the homophilic binding activity of C-cadherin , 1996, The Journal of cell biology.

[17]  Yi-Lin Yan,et al.  Double fluorescent in situ hybridization to zebrafish embryos. , 1996, Trends in genetics : TIG.

[18]  T. Bouwmeester,et al.  Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer , 1996, Nature.

[19]  C. Kimmel,et al.  Specification of cell fates at the dorsal margin of the zebrafish gastrula. , 1996, Development.

[20]  J. Campos-Ortega,et al.  Expression domains of a zebrafish homologue of the Drosophila pair-rule gene hairy correspond to primordia of alternating somites. , 1996, Development.

[21]  Shintaro T. Suzuki Structural and functional diversity of cadherin superfamily: Are new members of cadherin superfamily involved in signal transduction pathway? , 1996, Journal of cellular biochemistry.

[22]  E. Plow,et al.  The Cytoplasmic Domain of , 1996, The Journal of Biological Chemistry.

[23]  A. Fainsod,et al.  Overexpression of the homeobox gene Xnot-2 leads to notochord formation in Xenopus. , 1996, Developmental biology.

[24]  B. Gumbiner,et al.  Cell Adhesion: The Molecular Basis of Tissue Architecture and Morphogenesis , 1996, Cell.

[25]  J. Postlethwait,et al.  Cell-autonomous shift from axial to paraxial mesodermal development in zebrafish floating head mutants. , 1995, Development.

[26]  J. Postlethwait,et al.  A homeobox gene essential for zebrafish notochord development , 1995, Nature.

[27]  C. Kimmel,et al.  Stages of embryonic development of the zebrafish , 1995, Developmental dynamics : an official publication of the American Association of Anatomists.

[28]  C. Nüsslein-Volhard,et al.  no tail (ntl) is the zebrafish homologue of the mouse T (Brachyury) gene. , 1994, Development.

[29]  E M De Robertis,et al.  Expression of zebrafish goosecoid and no tail gene products in wild-type and mutant no tail embryos. , 1994, Development.

[30]  H. Okamoto,et al.  Developmental regulation of Islet‐1 mRNA expression during neuronal differentiation in embryonic zebrafish , 1994, Developmental dynamics : an official publication of the American Association of Anatomists.

[31]  R. Ho,et al.  Induction of muscle pioneers and floor plate is distinguished by the zebrafish no tail mutation , 1993, Cell.

[32]  R. Heimark,et al.  Protocadherins: a large family of cadherin‐related molecules in central nervous system. , 1993, The EMBO journal.

[33]  R. Beddington,et al.  Chimeric analysis of T (Brachyury) gene function. , 1993, Development.

[34]  D. Grunwald,et al.  Lithium perturbation and goosecoid expression identify a dorsal specification pathway in the pregastrula zebrafish. , 1993, Development.

[35]  Carmen R. Domingo,et al.  Pintallavis, a gene expressed in the organizer and midline cells of frog embryos: involvement in the development of the neural axis. , 1992 .

[36]  R. Ho,et al.  Cell-autonomous action of zebrafish spt-1 mutation in specific mesodermal precursors , 1990, Nature.

[37]  R. Kemler,et al.  The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species. , 1989, The EMBO journal.

[38]  C. Kimmel,et al.  A mutation that changes cell movement and cell fate in the zebrafish embryo , 1989, Nature.

[39]  S. Meier Development of the chick embryo mesoblast: morphogenesis of the prechordal plate and cranial segments. , 1981, Developmental biology.

[40]  S. Meier Development of the chick embryo mesoblast. Formation of the embryonic axis and establishment of the metameric pattern. , 1979, Developmental biology.

[41]  J. Gerhart,et al.  Formation and function of Spemann's organizer. , 1997, Annual review of cell and developmental biology.

[42]  M. Allende,et al.  Developmental regulation of zebrafish MyoD in wild-type, no tail and spadetail embryos. , 1996, Development.

[43]  M. Takeichi Morphogenetic roles of classic cadherins. , 1995, Current opinion in cell biology.

[44]  Solnica Mutations Affecting Cell Fates and Cellular Rearrangements during Gastrulation in Zebrafish Mutations Affecting Cell Fates and Cellular Rearrangements during Gastrulation in Zebrafish , 2022 .

[45]  M. Westerfield,et al.  ‡ Author for correspondence , 2022 .